• Tuberculosis and Respiratory Diseases
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• v.46 no.2
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• pp.185-194
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• 1999

Background: NF-$\kappa$B is a characteristic transcriptional factor whose functional activity is determined by post-translational modification of protein and subsequent change of subcellular localization. The involvement of the NF-$\kappa$B family of the transcription factors in the control of such vital cellular functions as immune response, acute phase reaction, replication of certain viruses and development and differentiation of cells has been clearly documented in many previous studies. Several recent observations have suggested that the NF-$\kappa$B might also be involved in the carcinogenesis of some hematological and solid tumors. Investigating the possibility that members of the NF-$\kappa$B family participate in the molecular control of malignant cell transformation could provide invaluable information on both molecular pathogenesis and cancer-related gene therapy. Method: To determine the expression patterns and functional roles of NF-$\kappa$B family transcription factors in human lung cancer cell lines NCI-H792, NCI-H709, NCI-H226 and NCI-H157 were analysed by western blot, using their respective antibodies. The nuclear and the cytoplasmic fraction of protein extract of these cell lines were subsequently obtained and NF-$\kappa$B expression in each fraction was again determined by western blot analysis. The type of NF-$\kappa$B complex present in the cells was determined by immunoprecipitation. To detect the binding ability of cell-line nuclear extracts to the KB consensus oligonucleotide, electrophoretic mobility shift assay(EMSA) was performed. Results: In the cultured human lung cancer cell lines tested, transcription factors of the NF-$\kappa$B family, namely the p50 and p65 subunit were expressed and localized in the nuclear fraction of the cellular extract by western blot analysis and immunocytochemistry. Immunoprecipitation assay showed that in the cell, the p50 and p65 subunits made NF-$\kappa$B complex. Finally it was shown by Electrophoretic Mobility Shift Assay(EMSA) that nuclear extracts of lung cancer cell lines are able to bind to NF-$\kappa$B consensus DNA sequences. Conclusion: These data suggest that in human lung cancer cell lines the NF-$\kappa$B p50/p65 complex might be activated. and strengthen the hypothesis that NF-$\kappa$B family transcription factors might be involved in the carcinogenesis of human lung cancer.

• Asian Journal of Turfgrass Science
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• v.18 no.3
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• pp.129-139
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• 2004

This study conducted to find out the best way to prolong green period of Zoysiagrass and Cool-season grass from 1999 to 2001 in Korea. Treatments were control, vinyl, black shade net, green shade net, black shade net+vinyl, green shade net+vinyl, vinyl+black shade net, and vinyl+green shade net for Zoysiagrass. For cool-season grass, treatments were control, non-punched vinyl and punched vinyl. Data on temperature, light intensity, humidity, leaf color and chlorophyll were collected. Plot size was 2 by 2 and the experiment was conducted using completely randomized design with three replication. Zoysiagrass Green period of Zoysiagrass depended on temperature and light intensity than humidity. Effective method of prolongation of green period in Zoysiagrass was to covered it with vinyl during early October to early November, and then using vinyl+black shade net from end of November. Cool-season grass Effective method of prolongation of green period in Cool-season grass was by covering it with vinyl during mid-November to mid-December, and then using vinyl+black shade net from end of December.

• Korean Journal of Poultry Science
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• v.39 no.2
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• pp.133-141
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• 2012

This study was carried out to investigate the performance of laying period of two-way crossbred of Korean native chickens. A total of four hundred eighty female 2-crossbred chicks that restored strains and were aboriginal at National Institute of Animal Science. Treatments were four crossbreds (4 replications/crossbred, 30 birds/replication) as A) C strain ${\times}$ S strain, B) C strain ${\times}$ H strain, C) R strain ${\times}$ S strain, and D) L strain ${\times}$ H strain, respectively. There were no significant difference on age, egg weight, and body weight at first egg among crossbreds (P>0.05). Body weight of D crossbred was lower compared to other crossbreds at the age of 24, 32, 40 and 60 week (P<0.05). Feed intake of A crossbred was highest among crossbreds (P<0.05). Weekly egg weight of A crossbred was the highest among crossbreds all laying period except 48~52 and 60~64 weeks. Hen-house egg production of A and B crossbreds was higher than that of C and D crossbreds (P<0.05). Hen-day egg production decreased after at the age of 36 weeks. Weekly egg production of A and B crossbreds was higher compared to C and D crossbreds (P<0.05). These results suggested the basic data on the record of laying period of 2-crossbreed Korean Native Chickens.

• Korean Journal of Poultry Science
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• v.39 no.3
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• pp.177-182
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• 2012

This work was carried out to investigate the performance of growing period of two-way crossed of Korean native chickens parental stocks. A total of four hundred eighty female 2-crossbred chicks were used in this study and they were from National Institute of Animal Science. Groups were four crossbreds (4 replications/crossbred, 30 birds/replication) as A) C strain ${\times}$ Y strain, B) C strain ${\times}$ L strain, C) C strain ${\times}$ G strain and D) C strain ${\times}$ W strain. Body weight of A crossbred was the highest at the age of 8 week (P<0.05) and that of D strain was the lowest for growing period (P<0.05). Body weights of A and B crossbreds were higher than those of C and D crossbreds at the 12 and 16 weeks (P<0.05). Weekly body weights of A and B crossbreds were higher than C and D crossbreds (P<0.05), and weekly body weight of B crossbred was higher compared to other crossbreds at 0~20 weeks old. Weekly feed intake of D crossbred was the lowest among all crossbreds at 0~12, 0~16 and 0~20 weeks old (P<0.05). Weekly feed conversion ratio of C crossbred was the highest among crossbreds (P<0.05). These results can give the basic information for growth related data in 2-way crossbreed Korean Native Chickens, which can be used for the parental stocks for the laying-type of Korean native commercial chickens.

• Korean Journal of Environmental Agriculture
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• v.27 no.4
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• pp.435-440
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• 2008

Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. The major causative agents of bovine mastitis include Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae), Staphylococcus aureus (S. aureus). These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Recently, many cases of mastitis are derived from environmental contamination of micro-organisms, which important factors for the spread of this disease in farm. Ultraviolet irradiation (UV) has been used as disinfection for waste and water in clinical and industrial facilities. Moreover the UV irradiation has been used as useful bactericidal agents to remove bacterial biofilms in environmental condition. In this study, we determined the bacterial replication in different percentage of water content (PWC) in sterilized saw dust and feces complexes from farm, and results showed that slightly decreased growth pattern of E. coli and S. agalactiae but increased growth pattern of S. aureus in various PWC (200, 400 and 600%) until 144 h incubation. In the bacteriocidal effect of UV irradiation to bacteria in saw dust and feces complex, the results showed that bacteriocidal effect was depended on the UV irradiation time, irradiation distance and PWC. Especially the antibacterial activity of UV irratiation is stronger in low PWC (50%), long time irradiation (50 sec), and short distance (5 cm) than other condition of this study. Furthermore UV irradiation with stirring showed increased the bactericidal effect compared without stirring. These results suggested that bovine mastitis causing agents may survive long time in environmental condition especially saw dust and feces complexes in farm and can cause a various disease including mastitis. Moreover, these data can be used as basis for application and development of UV disinfection to control of bovine mastitis from environmental contaminated bacteria in dairy farm.

• Korean Journal of Poultry Science
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• v.39 no.3
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• pp.245-252
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• 2012

This study was carried out to investigate the performance of laying period of two-crossbred of Korean native chickens for producing laying hens. A total of four hundred eighty female 2-crossbred chicks that restored strains and were aboriginal at National Institute of Animal Science. There were four crossbreds (4 replications/crossbred, 30 birds/replication) as A) C strain ${\times}$ Y strain, B) C strain ${\times}$ L strain, C) C strain ${\times}$ G strain, and D) C strain ${\times}$ W strain, respectively. There were no significant difference on age at first egg among crossbreds (P>0.05). Egg weight and body weight of B crossbred at first egg was higher than other crossbreds (P<0.05). Body weight of B crossbred was the highest 20 to 72 weeks (P<0.05), and C and D crossbreds were lower compared to A and B crossbreds (P<0.05). Average feed intake of B crossbred was the highest among crossbreds (P<0.05), and that of A crossbred was higher compared to C and D crossbreds (P<0.05). Weekly feed intake of four crossbreds decreased from 50 weeks. Weekly egg weight of B crossbred was the highest and that of D crossbred was the lowest among crossbreds (P<0.05). Feed conversion ratio of A and B crossbreds was lower than that of C and D crossbreds. Hen-house egg production of C crossbred was the highest among crossbreds until 26 weeks old (P<0.05), but there was no significant difference among crossbreds from at the age of 26 weeks (P>0.05). Hen-day egg production decreased after at the age of 38 weeks. Weekly egg production of A and B crossbreds was higher compared to C and D crossbreds at the age of 68~72 weeks (P<0.05). These results suggested the basic data on the record of laying period of 2-crossbreed Korean Native Chickens for producing laying hens.

• Korean Journal of Poultry Science
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• v.39 no.2
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• pp.143-149
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• 2012

This work was carried out to investigate the performance of growing period of large-type Korean native ducks. A total of four hundred twenty female and male duck chicks generated from A and B strains of ducks. Groups were four crossbreds (3 replications/crossbred, 35 birds/replication) as $2{\times}2$ factors (2 strains, A and B; 2 genders, female and male). On the body weight, male of B strains was the highest among 4 treatments after the age of 4 week (P<0.05). Body weight of B strain was higher than that of A strain at all period (P<0.05). Body weight of male was higher compared to that of female after the age of 4 week (P<0.05). On the body weight gain, male of B strain was the highest and female of A strain was the lowest among 4 treatments at 0~8, 0~12, 0~16 and 0~20 weeks (P<0.05). Body weight gain of male was higher than that of female after the age of 4 week (P<0.05). On the feed intake, male of B strain was the highest among 4 treatments from the age of 0 week and to the age of 4, 12, 16 and 20 week (P<0.05). Feed intake of male was higher than that of female at 0~12, 0~16 and 0~20 weeks (P<0.05). On the feed conversion ratio, male of B strain was the highest among other treatments 0~4 weeks and female of A strain was at 0~8 and 0~12 weeks (P<0.05). Feed intake of female was higher compared to that of female at 0~8 weeks and male was high at 0~20 weeks. These results provided the basic data on the record of growing phase of large-type Korean native ducks.

• Korean Journal of Poultry Science
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• v.47 no.3
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• pp.159-167
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• 2020

This study investigated the effect of different light sources in the poultry house on performance, meat quality, and blood composition of finishing broilers. Two hundred and forty male broilers (1-day-old, 42.2±0.1 g) were divided into three groups and subjected to different light source treatments (incandescent, LED, and fluorescent lamps) from 3 weeks of age (four replications/treatment, 20 birds/replication). After breeding for 6 weeks, the carcass yield and meat quality of broilers with similar body weight (BW; 3.4±0.07 kg) were investigated, and blood components were analyzed. Corn-soybean meal-based feed was provided as starter (CP 22.5%, ME 3,020 kcal/kg), early (CP 18.5%, ME 3,050 kcal/kg), and finishing (CP 18%, ME 3,100 kcal/kg). Performance, carcass yield, meat quality, and blood components were evaluated. BW, BW gain, feed intake, and feed conversion ratio did not show any significant differences among treatments. There was no significant difference on live weight and carcass yield among treatments. There was no significant difference on meat color, shear force, and water holding capacity; however, cooking loss at 17.2% was the highest in the LED treatment (P<0.05). There was no significant difference on blood components except for glucose (blood biochemistry component) among treatments. Glucose was 234.5 mg/dL, 256.9 mg/dL, and 250.1 mg/dL in the three treatments, respectively, with a significant difference between incandescent and LED treatments (P<0.05). These results are used useful as basic data for investigating the effect of lighting in broilers production.

• Korean Journal of Poultry Science
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• v.39 no.4
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• pp.305-310
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• 2012

This work was carried out to investigate performance and carcass yield of large-type broilers at different stocking densities. Treatments were T1 (9.1 birds/$m^2$), T2 (10.3 birds/$m^2$) and T3 (11.5 birds/$m^2$) by the stocking density. Four hundred eight 1-day-old Arbor Acre broiler chicks were used for six weeks (starter, 0~1 wks; earlier, 1~3 wks; finisher, 3~6 wks) and divided into 3 treatments (4 replications/treatment, 30, 34 or 38 birds/replication). Research indexes were rearing viability ratio, body weight, body weight gain, feed intake, feed conversion ratio, production efficiency factor and carcass ratio. Rearing viability ratio (%) was 89% or more for all treatments and there was no significant difference on weekly rearing viability ratio (%). Body weight of T2 was the greatest and that of T3 was the lowest at 1 weeks old (P<0.05). Body weight gain of T2 was the greatest and that of T3 was the lowest at 0~1 weeks old (P<0.05). However, body weight gain of T3 was the greatest and that of T1 was the lowest at 1~2 weeks old (P<0.05). Body weight gain of T2 was the greatest as 3,031 g among treatments at 0~6 weeks old (P<0.05). Feed intakes of T1, T2 and T3 were 1,417 g, 1,265 g and 1,355 g, respectively, and that of T1 was the greatest among treatments (P<0.05). There was no significant difference on body weight, body weight gain and feed intake. Feed conversion ratio of T1 was the greatest among treatments at 1~2 wks, 3~4 wks and 0~6 wks old (P<0.05). Production efficiency factors of T1, T2 and T3 were 363.5, 388.3 and 358.3, respectively, and there was no significant difference among treatments. Wing meat ratio of T1 was the higher compared to other treatments at the age of 4 wk (P<0.05). There was no significant difference on carcass ratio and partial meat ratio among treatments. Neck meat ratio of T2 was the lowest among treatments (P<0.05). This result may provide the standard data of different stocking densities for large-type broiler and the further research is needed.