• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 2002년도 제27회 정기총회, 제40회 학술발표회
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• pp.20-20
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• 2002

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 2001년도 제25회 정기총회 프로그램 제39회 학술발표회 및 특별강연 초록(한국초지학회)
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• pp.89.1-89
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• 2001

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 2003년도 제28회 정기총회, 제41회 학술발표회.심포지엄
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• pp.168-169
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• 2003

• Asian-Australasian Journal of Animal Sciences
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• 제16권1호
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• pp.83-87
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• 2003

The study evaluates the enzymatic dry matter (DM) degradability and water holding capacity of leaf and stem fractions of orchardgrass (Dactylis glomerata L.) at different growth stages with or without the presence of surfactant Tween 80. While Tween 80 significantly (p<0.05) increased water and enzyme holding capacities in the leaf blades fraction, less was observed in the fraction of leaf sheath and stem of orchardgrass. The enzyme holding capacity in the leaves was also altered more than that for water holding capacity. This resulted in the increased rate and extent of enzymatic hydrolysis of the leaf blade fractions at two growth stages, whereas little was with leaf sheath and stem fractions. It was also observed that at 0.005% concentrations of Tween 80 the enzymatic DM degradability of young leaf blades was higher (p<0.05) by 20-30% compared to that of the control, as well as for water and enzyme holding capacity. For matured leaf blades the DM degradability were increased with over 0.01% concentrations of the surfactant, but the increase was less than leaf blades of young orchardgrass. This result suggests the possibility of using the surfactant Tween 80 to improve forage digestibility in the rumen.

• 한국초지조사료학회지
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• 제8권3호
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• pp.104-109
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• 1988

A field experiment was conducted in order to investigate the seasonal changes of leaf growth and related characteristics in three cultivars of orchardgrass; Potomac, Kay and Sumas. The results were summarized as follows: 1. Leaf elongation was increased in a nearly linear phase during first and third cutting stages. It was increased slowly in early 10 days to 15 days after cutting and increased rapidly thereafter during the rest cutting stages. In cultivars, Potomac was showed hlgher leaf elongation than other cultivars during all cutting stages. There was no difference of leaf width within cutting stages, but the leaf width of fall regrowth was narrow. Sumas had relatively short and wide leaves. 2. Leaf dry weight and leaf area in first cutting stage were larger than others. Leaf area was increased rapidly from 15 days after cutting and leaf $we$ was increased rapidly from 20 days over all cutting stages. The increase in leaf area and dry weight were slow down after 30 days. 3. Number of epidermal cells was increased rapidly after cutting and the rate of increase was slow down after 30 days. In a cross section of leaf tissue, the part of mesophyll was occupied with about 60% of total area and larger area than other tissues. Leaf tissue had a large vacancy at early growth period after harvest and was filled gradually with mesophyll. This result was related to the increase of leaf dry matter.

• 한국초지조사료학회지
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• 제21권1호
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• pp.21-26
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• 2001

환경 스트레스에 의해 야기되는 활성 산소종에 의한 피해에 내성을 가지는 목초의 개발을 위하여 오차드그래스의 배반 조직 유래의 캘러스에 배추유래의 cytosolic glutathione reductase 유전자(BcGRl)를 Agrobucterium tumefaciens EHA101을 매개로 형질전환시켰다. Hygromcin으로 선발된 캘러스로부터 재분화된 식물체는 야생형과 비교하여 형태적으로 차이를 나타내지 않았다. PCR 및 Southern blot 분석을 통하여 형질전환 식물체의 염색체 내에 BcGRl 유전자가 integration 되었음을 확인하였다. 오차드그래스의 잎으로부터 total RNA를 분리하여 Northern blot 분석을 실시한 결과, 도입된 유전자가 형짙전환 식물체 내에서 지속적으로 발현된다는 것을 확인하였다.

• 한국초지조사료학회지
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• 제20권4호
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• pp.259-264
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• 2000

오차드그래스의 완숙 종자배양에서 배지 내에 첨가되는 copper의 농도가 캘러스 형성과 식물체 재분화율에 미치는 영향을 조사하였다. 캘러스의 유도는 $2\;mg/{\ell}$의 2,4-D와 $0.1-100\;{\mu}mol$의 copper sulfate를 첨가한 MS 배지를 이용하였으며, 식물체 재분화는 $1\;mg/{\ell}$의 NAA, $5\;mg/{\ell}$의 kinetin 및 $0.1-100\;{\mu}mol$의 copper sulfate가 첨가된 N6 배지를 이용하였다.그 결과, copper의 첨가는 캘러스 형성능력에는 유의성있는 차이를 나타내지 않았으나, copper가 첨가된 배지에서 생성된 캘러스에서 백색이면서 조직이 치밀한 즉, 식물체 재분화에 적합한 캘러스의 비율이 높게 나타났다. 식물체 재분화율은 copper가 첨가된 배지에서 생성된 캘러스가 첨가되지 않은 배지에서 생성된 캘러스보다 높게 나타났으며, 특히 $70\;{\mu}mol$의 copper가 첨가된 백지에서 생성된 캘러스가 대구조에 비해 2.5배 이상 증가된 가장 높은 재분화 능력을 나타내었다. 또한, 캘러스당 재분화된 식물체의 수는 $70\;{\mu}mol$의 copper가 첨가된 배지에서 생성된 캘러스에서 대조구에 비해 2.6배 이상 증가된 것으로 나타났다.

• 한국초지조사료학회지
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• 제14권3호
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• pp.215-222
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• 1994

This experiment was conducted to find out the response of orchardgrass (Dactylis glorneruta L.) varieties to grazing at the condition of monocultures and mixed pastures on the field of Livestock Experiment Station at Suwon from Sep. 1989 to Oct. 1991. The varieties of orchardgrass used in this experiment were Potomac, Hallmark and Syn. No. 2 as a early maturing type, and Lidacta, Rancho and Syn. No. 10 as a late maturing type. The results obtained were as follows: 1. Herbage utilization of mixture with Hallmark variety(T2) was the highest as 78.2% and that with Rancho variety(T5) was the lowest as 70.2%. but there was no difference(P>0.05). Mixtures with early maturing varieties(T1, T2 and T3) were higher as 77.4% than those with late varieties(T4, T5 and T6) as 72.9% in herbage utilization. In monocultures Rancho variety(T5) was the highest as 68.6% and Lidacta variety(T4) was the lowest as 58.8%, and mixtures with early maturing varieties(T1, T2 and T3) as 66.6% were higher than those of late(T1, 72 and 73) as 64.6%. 2. The content of crude protein of 6 mixed pastures ranged from 19.7% to 22.8%, and those of ether extracts, crude fiber, crude ash and nitrogen free extract were 6.6 to 7.5%, 24.9 to 26.2'76, 1 1.5 to 1 1.9% and 33.6-35.4 8, respectively, But there were not different significantly(P>0.05). In monocultures, significant difference by variety was observed in crude protein content(P<0.05). 3. In botanical composition, there was just a little different among 6 mixtures, having 90% or more in grass percentage. The percentages of each orchardgrass varieties were decreased, but those of perennial ryegrasses were increased as grazing sesson was progressed in all treatment.

• 한국초지조사료학회지
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• 제14권3호
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• pp.206-214
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• 1994

This experiment was conducted to find out the response of orchardgrass (Dactylis glomrrata L.) varieties to grazing at the condition of monocultures and mixed pastures on the field of Livestock Experiment Station at Suwon from Sep. 1989 to Oct. 1991. The varieties of orchardgrass used in this experiment were Potomac, Hallmark and Syn. No. 2 as a early maturing type, and Lidacta, Rancho and Syn. No. 10 as a late maturing type. The results obtained were as follows: I. Heading date of early maturing varieties among orchardgrass like as Potomac, Hallmark and Syn. 2 ranged from May 9 to May 14. Otherwise those of late varieties(Lidacta Rancho and Syn. 10) were between May 21 to May 25. Leaf types of early varieties were shown erect, those of late varieties were erect or semi-erect. 2. In dry matter production per ha, mixture with Rancho variety(T5) was the highest as 7.818 kg and mixture with Hallmark(T2) was the lowest as 7,013 kg. But there was not significantly different(P>0.05) among mixtures. While in monocultures, Syn. 10 variety was the highest(6.626 kglha) and Lidacta was Iowest(5,775 kg). But no difference was found(P>0.05). 3. In mean grass height after grazing, mixtures with early maturing types were higher as 9.8 cm than that of late (8.8cm), and early types were higher as 10.5cm than that of late(9.4cm) in monocultures. But there was no significant difference both mixtures and monocultures.

• Asian-Australasian Journal of Animal Sciences
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• 제21권5호
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• pp.657-662
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• 2008

To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, the recombinant DgHSP17.2 gene was introduced into orchardgrass plants using the Agrobacterium-mediated transformation method and expressed constitutively under the control of the CaMV 35S promoter. The results of genomic DNA PCR and Southern analysis showed a DNA band and hybridization signal on agarose gel and X-ray film in transgenic orchardgrass plants harboring the recombinant DgHSP17.2 gene, but a DNA band and hybridization signal were not observed in the wild type and empty vector control plants. The same result was also obtained in RT-PCR and Southern blot analysis, and these transgenic orchardgrass plants did not show any morphological aberration both in the culture bottle and soil mixture. When leaf discs cut from transgenic orchardgrass plants with recombinant DgHsp17.2 gene were exposed to lethal temperature (heat treatment at $60^{\circ}C$ for 50 min), 60-80% of the leaf discs showed only damage symptoms, but non-transgenic leaf discs showed a lethal condition. These results indicate that the DgHsp17.2 gene may act as a protector from heat stress in plants.