Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Acquisition of Thermotolerance in Transgenic Orchardgrass Plants with DgHSP17.2 Gene

  • Kim, Ki-Yong (National Institute of Animal Science) ;
  • Jang, Yo-Soon (Korea Ocean Research and Development Institute) ;
  • Cha, Joon-Yung (Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Son, Daeyoung (Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Choi, Gi Jun (National Institute of Animal Science) ;
  • Seo, Sung (National Institute of Animal Science) ;
  • Lee, Sang Jin (National Institute of Animal Science)
  • Received : 2006.12.14
  • Accepted : 2007.12.11
  • Published : 2008.05.01
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Abstract

To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, the recombinant DgHSP17.2 gene was introduced into orchardgrass plants using the Agrobacterium-mediated transformation method and expressed constitutively under the control of the CaMV 35S promoter. The results of genomic DNA PCR and Southern analysis showed a DNA band and hybridization signal on agarose gel and X-ray film in transgenic orchardgrass plants harboring the recombinant DgHSP17.2 gene, but a DNA band and hybridization signal were not observed in the wild type and empty vector control plants. The same result was also obtained in RT-PCR and Southern blot analysis, and these transgenic orchardgrass plants did not show any morphological aberration both in the culture bottle and soil mixture. When leaf discs cut from transgenic orchardgrass plants with recombinant DgHsp17.2 gene were exposed to lethal temperature (heat treatment at $60^{\circ}C$ for 50 min), 60-80% of the leaf discs showed only damage symptoms, but non-transgenic leaf discs showed a lethal condition. These results indicate that the DgHsp17.2 gene may act as a protector from heat stress in plants.

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References

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