• Title/Summary/Keyword: DS/S

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Electron Microscopic Visualization of Dendrolimus spectabilis Midgut Cells infected by D. spectabilis Nuclear Polyhedrosis Virus (Dendrolimus spectabilis Nuclear Polyhedrosis Virus에 감염된 솔나방유충 중장세포의 전자현미경적 관찰)

  • Lee, Hyung-Hoan;Lee, Keun-Kwang;Lee, Min-Woong
    • Applied Microscopy
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    • v.21 no.1
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    • pp.21-26
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    • 1991
  • Midgut cells of the dead Dendrolimus spectabilis larvae by infection of D. spectabilis nuclear polyhedrosis virus (DsNPV) were observed by transmission electron microscopy. DsNPV replicated in the nucleus of the infected midgut cells and the virogenic stroma of DsNPV appeared in the nucleus, from which nucleocapsids were formed. Also the formation of polyhedral inclusion bodies were observed in the nucleus.

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A performance analysis of the discrete time DS/CDMA system based on the code phase difference (코드 위상차에 따른 이산 시간 CDMA 시스템의 성능 분석)

  • 안병양
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.35S no.5
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    • pp.11-16
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    • 1998
  • DS/CDMA systems for the high speed communication require high code rats. In discrete time CDMA receivers, the performance degradation, caused by the phase difference between transmission code and reference code, increase the sampling frquency of the receiver. This increment of the sampling frequency makes hard to implement high speed CDMA systems. This paper analyzes the SIR(signal to interference Ratio) performance of the discrete time DS/CDMA system, based on the code phase difference. The results of this paper may be useful to study a low-sampling CDMA receiver.

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Influencing Factors on the Dietary supplements Consumption among Children in Korea (우리나라 어린이 및 청소년의 식이보충제 섭취에 영향을 미치는 요인)

  • Lee, Jee-Yeon;Kim, Do-Hee;Lee, Yoon-Na;Koh, Eun-Mi;Jang, Young-Soo;Lee, Hye-Young;Jang, Young-Ae;Kim, Cho-Il
    • Korean Journal of Community Nutrition
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    • v.16 no.6
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    • pp.740-750
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    • 2011
  • With a recent increase in dietary supplements (DS) consumption among children in Korea, this study was performed to examine the influencing factors on children's DS consumption. A nationwide survey was conducted employing 3 representative samples of children for summer & fall of 2008 and spring of 2009 by stratified multistage sampling of 120 survey sites per season based on the 2005 census population. Approximately 30 households from each survey site were screened for residing children of 0-19 years and about 1,700 households remained as eligible samples per season. Trained dietitians visited households to perform face-to-face interview to children and/or parents regarding DS consumption including health functional foods (HFF), vitamins/minerals (V/M) supplements and other food supplements during 1 month prior to interview. Out of 5,328 children responded, 18.7% reported DS consumption. Consumption rate was higher in boys (19.9% vs. 17.3% in girls, P < 0.05) and youngsters (22.8% compared to 15.0% in adolescents, P < 0.001). Children from higher income family (P < 0.001), those living in apartments (P < 0.001), those residing in metropolitan area (P < 0.001), and those of mothers with higher education (P < 0.001) were more likely to take DS. Also, mother's employment status and occupation were significantly associated with children's DS consumption. The most popular DS was HFF (72.1%), which was consumed more in children of higher income family. It is revealed that socioeconomic factors affect children's DS consumption significantly. Also it is necessary to estimate children's V/M intake from DS and foods together especially because there are tolerable upper limits set for V/M for safety purposes.

Characterization of Rice black-streaked dwarf virus in Maize (옥수수에 발생하는 벼검은줄오갈병의 유전자 비교)

  • Lee, Bong-Choon;Yoon, Young-Nam;Hong, Sung-Jun;Hong, Yeon-Kyu;Hwang, Jae-Bok;Song, Sek-Bo;Kang, Hwang-Won;Lee, Key-Woon
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.223-225
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    • 2008
  • This study was carried out to identify the Rice black-streaked dwarf virus that infected maize plants collected from Gochang-gun in Jeollabukdo in 2005. The genomic dsRNA from infected plants was extracted and the genome pattern was analyzed by polyacrylamide gel electrophoresis. Results of the electrophoresis revealed the already known to-segment genome and the difference of mobility was confirmed in isolates by collected areas. The RBSDV was identified from the result of RT-PCR using the template of extracted dsRNA and specific primer. The results of S10 cloned to pGEM-T vector and the conducted in sequence analysis consisted of 1,801nt and 559aa. This was of the same size as the RBSDV S10 identified in rice, and the change was confirmed in 18 base and displayed homology of 99%.

Evaluation of High Temperature Tensile Properties in GTD-111DS (GTD-111DS 소재의 고온 인장 특성 평가)

  • Park H.S.;Kim H.I.;Lee Y.M.;Seok C.S.;Kim M.Y.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.1358-1362
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    • 2005
  • The Ni-base superalloy GTD-111DS was designed in the 1970s and is widely used as the material of the first stage blade under a severe combination of temperature and pressure in gas turbines. But because GTD-111DS is distributed in the shape of blade and blade has a unique figure and many cooling channels, it is hard to manufacture the test specimen. In this reason, there are little data on the microstructure and mechanical properties of the alloy. Therefore through the microstructure analysis, present paper observed that the shape of $\gamma{'}$ did not change even if aging time was increased but the amount and volume of the deposition of secondary $\gamma{'}\;rose\;and\;secondary\;\gamma{'}\;grew\;among\;primary\;\gamma{'}$. Also, by tensile test for different temperature, there was difference between yield strength and tensile strength in room temperature on heat treatment and extracting region but the more increasing temperature, the more decreasing difference between yield strength and tensile strength.

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Characteristics of Flux Decline in Forward Osmosis Process for Asymmetric Cellulose Membrane (정삼투 공정에 있어 비대칭 셀룰로오즈 막의 투과유속 감소특성)

  • Lee, Keun-Woo;Han, Myeong-Jin;Nam, Suk-Tae
    • Korean Chemical Engineering Research
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    • v.52 no.3
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    • pp.328-334
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    • 2014
  • This study examined the effect of concentration polarization on permeate flux in forward osmosis (FO) membrane process for saline and sucrose solution. The reduction in permeate flux during the FO membrane process is largely due to the formation of concentration polarization on membrane surfaces. The flux reduction due to internal concentration polarization formed on the porous support layer was larger than that due to the external concentration polarization on the active membrane surface. Water permeate flux through the FO membrane increased nonlinearly with the increase in osmotic pressure. The water permeability coefficient was $1.8081{\times}10^{-7}m/s{\cdot}atm$ for draw solution on active layer (DS-AL) mode and $1.0957{\times}10^{-7}m/s{\cdot}atm$ for draw solution on support layer (DS-SL) mode in NaCl solution system. The corresponding membrane resistance was $5.5306{\times}10^6$ and $9.1266{\times}10^6s{\cdot}atm/m$, respectively. With respect to the sucrose solution, the permeate flux for DS-AL mode was 1.33~1.90 times higher than that for DS-SL mode. The corresponding variation in the permeation flux (J) due to osmotic pressure (${\pi}$) would be expressed as $J=-0.0177+0.4506{\pi}-0.0032{\pi}^2$ for the forward and $J=0.0948+0.3292{\pi}-0.0037{\pi}^2$ for the latter.

Dietary Risk Assessment of Snf7 dsRNA for Coccinella septempunctata

  • Jung, Young Jun;Seol, Min-A;Choi, Wonkyun;Lee, Jung Ro
    • Proceedings of the National Institute of Ecology of the Republic of Korea
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    • v.2 no.3
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    • pp.210-218
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    • 2021
  • Recently, pest-resistant living modified (LM) crops developed using RNA interference (RNAi) technology have been imported into South Korea. However, the potential adverse effects of unintentionally released RNAi-based LM crops on non-target species have not yet been reported. Coccinella septempunctata, which feeds on aphids, is an important natural enemy insect which can be exposed to the double-stranded RNA (dsRNA) produced by RNAi-based LM plants. To assess the risk of ingestion of Snf7 dsRNA by C. septempunctata, we first identified the species through morphological analysis of collected insects. A method for species identification at the gene level was developed using a specific C. septempunctata 12S rRNA. Furthermore, an experimental model was devised to assess the risk of Snf7 dsRNA ingestion in C. septempunctata. Snf7 dsRNA was mass-purified using an effective dsRNA synthesis method and its presence in C. septempunctata was confirmed after treatment with purified Snf7 dsRNA. Finally, the survival rate, development time, and dry weight of Snf7 dsRNA-treated C. septempunctata were compared with those of GFP and vATPase A dsRNA control treatments, and no risk was found. This study illustrates an effective Snf7 dsRNA synthesis method, as well as a high-concentration domestic insect risk assessment method which uses dsRNA to assess the risk of unintentional released of LM organisms against non-target species.

Degradative Solidification/Stabilization of Liquid Waste Containing Chloroform and Methylene Chloride by Cement/Slag/Fe(II) Systems (Cement/Slag/Fe(II) 시스템에 의한 클로로포름과 메틸렌클로라이드 함유 액상폐기물의 분해성 고형화/안정화)

  • Seong, Joseph;An, Cheol-Hong;Choi, Won-Ho;Park, Joo-Yang
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.10
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    • pp.1034-1038
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    • 2008
  • Degradative Solidification/Stabilization(DS/S) is a modification of conventional Solidification/Stabilization(S/S) that incorporates degradative processes for organic contaminant destruction with the low cost of conventional S/S. Inorganic contaminants are immobilized and chlorinated organic contaminants are destroyed by DS/S treatment. In this study, a DS/S using cement/slag/Fe(II) systems as binder was investigated to assess its effectiveness in degrading chloroform(CF) and methylene chloride(MC) contained in hazardous liquid wastes. The initial concentration of CF was 0.26 mM, 1.0 mM, 8.4 mM, 25 mM and 42 mM and Fe(II) was 200 mM. The result showed that degradation of CF in various concentration was in one kind reaction as pseudo-first-order and 95% of 0.26 mM initial concentration of CF was removed in five days. 50 mg/L of heavy metal was added in order to accelerate the rate of degradation of MC and initial concentration of MC was 3.50 mM however, degradation did not occur in system. Thus additional studies needed for degradation of MC and more studies on other reaction pathways products will help elucidate reaction mechanisms and pathways for chlorinated methanes in cement/slag/Fe(II) systems.

A Freeze-drying Formulation and Target Specificity of Double-stranded RNA-expressing Bacteria to Control Insect Pests (Double-stranded RNA 발현 세균의 동결건조 제형화와 적용 대상 해충 선택성)

  • Kim, Eunseong;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.55 no.2
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    • pp.81-89
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    • 2016
  • Double-stranded RNA (dsRNA) has been applied to control insect pests by its suppressive activity against specific target genes. Integrin is a heterodimer (${\alpha}$ and ${\beta}$) transmembrane protein and plays a critical role in cell-to-cell or cell-to-extracellular matrix interactions in eukaryotes. Suppression of ${\beta}$ subunit integrin gene expression by its specific dsRNA (= dsINT) induces significant mortality against target insects. Furthermore, a recombinant bacterium expressing dsINT is potent to kill target insects. However, it is necessary to develop a formulation technique of the dsRNA-expressing bacteria to apply the bacterial insecticide against field populations. This study formulated the recombinant bacteria by freeze-drying and tested its control efficacy against target insects. The formulation maintained significant insecticidal activity against last instar larvae of Spodoptera exigua. While a commercial Bacillus thuringiensis (Bt) insecticide exhibited only about 60% insecticidal activity against S. exigua last instar, an addition of the dsINT-expressing bacterial formulation significantly enhanced the Bt insecticidal activity. The dsINT-expressing bacterial formulation exhibited relative selectivity to target insects depending on sequence similarity. These results indicate that a freeze-dried form of dsRNA-expressing bacteria keeps its insecticidal activity.

Experimental Investigation of Physical Mechanism for Asymmetrical Degradation in Amorphous InGaZnO Thin-film Transistors under Simultaneous Gate and Drain Bias Stresses

  • Jeong, Chan-Yong;Kim, Hee-Joong;Lee, Jeong-Hwan;Kwon, Hyuck-In
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.17 no.2
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    • pp.239-244
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    • 2017
  • We experimentally investigate the physical mechanism for asymmetrical degradation in amorphous indium-gallium-zinc oxide (a-IGZO) thin-film transistors (TFTs) under simultaneous gate and drain bias stresses. The transfer curves exhibit an asymmetrical negative shift after the application of gate-to-source ($V_{GS}$) and drain-to-source ($V_{DS}$) bias stresses of ($V_{GS}=24V$, $V_{DS}=15.9V$) and ($V_{GS}=22V$, $V_{DS}=20V$), but the asymmetrical degradation is more significant after the bias stress ($V_{GS}$, $V_{DS}$) of (22 V, 20 V) nevertheless the vertical electric field at the source is higher under the bias stress ($V_{GS}$, $V_{DS}$) of (24 V, 15.9 V) than (22 V, 20 V). By using the modified external load resistance method, we extract the source contact resistance ($R_S$) and the voltage drop at $R_S$ ($V_{S,\;drop}$) in the fabricated a-IGZO TFT under both bias stresses. A significantly higher RS and $V_{S,\;drop}$ are extracted under the bias stress ($V_{GS}$, $V_{DS}$) of (22 V, 20V) than (24 V, 15.9 V), which implies that the high horizontal electric field across the source contact due to the large voltage drop at the reverse biased Schottky junction is the dominant physical mechanism causing the asymmetrical degradation of a-IGZO TFTs under simultaneous gate and drain bias stresses.