Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of Rice black-streaked dwarf virus in Maize

옥수수에 발생하는 벼검은줄오갈병의 유전자 비교

  • 이봉춘 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 윤영남 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 홍성준 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 홍연규 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 황재복 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 송석보 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 강항원 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 이기운 (경북대학교)
  • Published : 2008.12.01
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Abstract

This study was carried out to identify the Rice black-streaked dwarf virus that infected maize plants collected from Gochang-gun in Jeollabukdo in 2005. The genomic dsRNA from infected plants was extracted and the genome pattern was analyzed by polyacrylamide gel electrophoresis. Results of the electrophoresis revealed the already known to-segment genome and the difference of mobility was confirmed in isolates by collected areas. The RBSDV was identified from the result of RT-PCR using the template of extracted dsRNA and specific primer. The results of S10 cloned to pGEM-T vector and the conducted in sequence analysis consisted of 1,801nt and 559aa. This was of the same size as the RBSDV S10 identified in rice, and the change was confirmed in 18 base and displayed homology of 99%.

2005년 전북 고참에서 채집한 옥수수 이병주로부터 벼검은줄오갈병 바이러스를 동정하였다. 이들 이병주로부터 게놈 dsRNA를 추출하여 polyacrylamide gel 전기영동으로 게놈 패턴을 분석 하였다. 전기영동 결과 이미 알려진 10개의 분절게놈을 확인하였으며 채집지역별 isolate에서 게놈 dsRNA이동도의 차이를 확인하였다. 추출된 dsRNA를 주형으로 하여 S10의 full-length 특이 primer를 사용하여 RT-PCR한 결과 1,801의 예상되는 band를 확인하여 RBSDV로 동정하였다. S10을 pGEM-T vector에 크로닝하여 염기서열 분석 결과 1,801nt, 559aa로 구성되어 있었다. 이는 벼에 발생하는 RBSDV S10의 크기와 동일하였으며 상동성 분석결과 18개 염기에서 변이가 확인되어 99%의 상동성을 나타내었다.

Keywords

References

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