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http://dx.doi.org/10.5423/RPD.2008.14.3.223

Characterization of Rice black-streaked dwarf virus in Maize  

Lee, Bong-Choon (Department of Functional Crop, NICS, RDA)
Yoon, Young-Nam (Department of Functional Crop, NICS, RDA)
Hong, Sung-Jun (Department of Functional Crop, NICS, RDA)
Hong, Yeon-Kyu (Department of Functional Crop, NICS, RDA)
Hwang, Jae-Bok (Department of Functional Crop, NICS, RDA)
Song, Sek-Bo (Department of Functional Crop, NICS, RDA)
Kang, Hwang-Won (Department of Functional Crop, NICS, RDA)
Lee, Key-Woon (Kyungpook National University)
Publication Information
Research in Plant Disease / v.14, no.3, 2008 , pp. 223-225 More about this Journal
Abstract
This study was carried out to identify the Rice black-streaked dwarf virus that infected maize plants collected from Gochang-gun in Jeollabukdo in 2005. The genomic dsRNA from infected plants was extracted and the genome pattern was analyzed by polyacrylamide gel electrophoresis. Results of the electrophoresis revealed the already known to-segment genome and the difference of mobility was confirmed in isolates by collected areas. The RBSDV was identified from the result of RT-PCR using the template of extracted dsRNA and specific primer. The results of S10 cloned to pGEM-T vector and the conducted in sequence analysis consisted of 1,801nt and 559aa. This was of the same size as the RBSDV S10 identified in rice, and the change was confirmed in 18 base and displayed homology of 99%.
Keywords
Genomic dsRNA; Nucleotide sequence; Rice black-streaked dwarf virus;
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Times Cited By KSCI : 2  (Citation Analysis)
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