• 제목/요약/키워드: DPPH $({\alpha},{\alpha}'-diphenyl-{\beta}-picrylhydrazyl)$

검색결과 102건 처리시간 0.039초

인삼으로부터 페놀성 항산화 성분의 분리 (Isolation of Phenolic Antioxidant Components from Panax ginseng)

  • 위재준;박종대;김만욱;이형주
    • Applied Biological Chemistry
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    • 제32권1호
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    • pp.44-49
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    • 1989
  • 인삼중 새로운 항산화 성분을 분리하기 위하여 인상의 메탄올 추출물을 여러 성분 분획으로 나눈 다음 ${\alpha}$, ${\alpha}-diphenyl-{\beta}-picrylhydrazyl(DPPH)$을 이용하여 각각의 항산화 활성을 측정하였다. DPPH 소거 활성이 비교적 큰 두 분획을 silica gel, ${\mu}-Bondapak$ $C_{18}$ 또는 Sephadex LH-20 column chromatograph하여 4중의 단일성분을 분리하였고 이들은 정색반응 및 UV분석을 통해 모두 페놀성 성분으로 확인되었다.

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Bacillus sp. FF-7에 의한 항산화물질 생산조건과 항산화 활성 (Antioxidative Activity and Produced Condition of Antioxidative Substance by Bacillus sp. FF-7)

  • 차재영;김효정;전방실;박진철;옥민;조영수
    • Applied Biological Chemistry
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    • 제46권3호
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    • pp.165-170
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    • 2003
  • 발효식품 시료로부터 분리된 세균을 $DPPH({\alpha},{\alpha}'-diphenyl-{\beta}-picrylhydrazyl)$ 전자공여능으로 항산화 활성을 측정하여 가장 활성이 강한 균주를 선별하여 형태학적, 생화학적, 생리학적 특성 및 165 rRNA 염기서열을 조사한결과 Bacillus sp.으로 판명되어 FF-7로 명명하였다. DPPH 전자공여능법에 의한 Bacillus sp. FF-7이 생산하는 항산화물질의 최적 생산 배지조건은 탄소원 2% galactose와 질소원 1% tryptone 첨가였다. Bacillus sp. FF-7에 의해 생성된 항산화 물질의 활성을 DPPH 전자공여능, 흰쥐 각 조직 microsomal 실험계 및 linoleic acid 과 산화지질 실험계에서 malondialdehyde를 thiobarbituric acid(TBA)방법으로 측정하였다. 흰쥐 각 조직 microsomal 실험계에서 지질과산화에 대한 항산화 효과는 뇌(97.50%) >심장(79.95%) >신장(77.84%) >비장(77.47%) >고환(69.96%) >간장(62.45%) 순이였다. Linoleic acid의 과산화지질를 TBA법으로 측정한 결과 반응 4일째까지 억제 효과가 강하게 나타났으며, 동시에 대조구로 사용한 0.05% BHT 첨가구에서도 실험종료시까지 항산화 활성이 강하게 나타났다.

Inhibitory Effects of Methanol Extract, Phenolic Acids and Flavonoids from the Leaves of Eucalyptus darylmpleana against 1,1-Diphenyl-2-picrylhydrazyl Radical

  • Park, Jong-Cheol;Park, Ju-Gwon;Hur, Jae-Seoun;Choi, Myeong-Rak;Yoo, Eun-Jeong;Kim, Sung-Hwan;Son, Jin-Chang;Kim, Moon-Sung
    • Natural Product Sciences
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    • 제10권5호
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    • pp.244-247
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    • 2004
  • The inhibitory effects of the leaves of Eucalyptus darylmpleana (Myrtaceae) on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was examined. The scavenging effect of the ethyl acetate fraction of Eucalyptus darylmpleana leaves on DPPH radical was stronger than the other fractions, and further purified by silica gel and Sephadex LH-20 column chromatography. 3,4-Dihydroxybenzoic acid, gallic acid, quercetin, quercetin $3-O-{\alpha}-_L-rhamnoside$, quercetin $3-O-{\beta}-_D-glucoside$ and quercetin 3-O-rutinoside were isolated and elucidated by spectroscopic data. Among these components, gallic acid and quercetin $3-O-{\alpha}-_L-rhamnoside$ exhibited potent scavenging activities on DPPH radical with $IC_{50}$ values of 6.02 and $5.54\;{\mu}M$, respectively.

Smilax riparia 잎의 항산화 성분 (The Anti-oxidative Compounds of Smilax riparia Leaves)

  • 조은선;김정일;김호현;전인주;함인혜;황완균
    • 약학회지
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    • 제47권5호
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    • pp.300-306
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    • 2003
  • Rhizoma of Smilax china has been used as anti-inflammatory and analgesic, antiedemic agent in Korean folk medicine. In order to investigate the efficacy of anti-oxidative activity, the activity-guided fractionation and the isolation were performed. Each fractions ($H_2O$ fraction, 20%, 40%, 60%, 100% MeOH fractions and CHCl$_3$ fraction) was examined antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging potential. It was revealed that 40%, 20% MeOH fractions and $H_2O$ fractions have significant anti-oxidative activity. From 40% and 20% MeOH fractions two flavonoid glycosides and one procyanidin were isolated and elucidated apigenin-7-Ο-$\alpha$-L-rhamnopyranosyl(1\longrightarrow2)-$\beta$-D-glucopyranoside, apigenin-7-Ο-$\alpha$-L-rhamnopyranosyl(1\longrightarrow6)-$\beta$-D-glucopyranoside and catechin(4$\alpha$\longrightarrow6)epicatechin through their physicochemical data and IR, FAB-MS, $^{13}$ C-NMR, and $^1$H-NMR analysis with authentics, respectively. The isolated compounds were examined by DPPH method. Apigenin-7-Ο-$\alpha$-L-rhamnopyranosyl(1\longrightarrow2)-$\beta$-D-glucopyranoside and catechin (4$\alpha$\longrightarrow6) epicatechin showed powerful radical scavenging activities on DPPH radical among three compounds.

솔잎 추출물의 항산화 효과 (Antioxidative Effects of Pine (Pinus denstifora) Needle Extracts.)

  • 유지현;차재영;정영기;정경태;조영수
    • 생명과학회지
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    • 제14권5호
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    • pp.863-867
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    • 2004
  • 식물성 성분 중에서 생리활성 인자를 탐색할 목적으로 솔잎의 수용성 추출물에 의한 항산화 활성을 DPPH법, thiocyanate법, TBA법 및 microsome 생 체 막 지 질 과산화물 생성정도를 TBARS법으로 측정 하고, 총 폴리페놀 함량을 측정하였다. 솔잎 수용성 추출물의 총 폴리페놀 화합물 함량은 1.61%이었으며, DPPH 측정법에서는 짙은 자색의 탈색되는 정도로 나타내는 전자 공여능이 솔잎 수용성 추출물의 0.1% 농도에서 BHT와 비슷한 수준으로 항산화 활성이 높게 나타났다. 간 microsome을 이용한 생체막 지질 과산화 억제정도는 솔잎 수용성 추출물의 0.1% (67.7%)>0.05% (63.1%)>0.01%(28.2%) 순으로 농도에 비례하여 증가하였다. Linoleic acid 산화 실험계를 이용한 thiocyanate법에서는 0.01%, 0.05%, 0.1%의 농도에서 모두 반응 7일째까지 대조구에 비해 매우 강한 항산화 활성을 보였으며, TBA법에서는 반응 6일째까지 는 0.1%>0.05%>0.01%의 순으로 농도 증가에 따라 항산화 활성도 높게 나타났다. 이상의 결과에서 솔잎 수용성 추출물 중에는 in vitro 항산화 실험에서 항산화 활성을 나타내는 생리활성 성분이 존재하는 것으로 볼 수 있으므로 천연 항산화제로서의 가능성을 시사하였다.

오배자 추출 폴리페놀류 처리 면섬유의 항산화성 분석 (Evaluation of Anti-oxidant Activity of Cotton fabrics treated with the Gallnut Extracts)

  • 장경진;손송이;정종석;김태경
    • 한국염색가공학회:학술대회논문집
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    • 한국염색가공학회 2008년도 제39차 학술발표회
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    • pp.79-80
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    • 2008
  • In order to introduce new functions onto textile fabrics, cotton was treated with gallnut extracts. And then the anti-oxidant activity of fabrics treated with the extracts was evaluated by Blois method using DPPH(${\alpha},{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl). The anti-oxidant activity was exhibited on the textile fabrics treated as well as by the extracts itself.

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적갓김치 Anthocyanins의 항산화 특성에 관한 연구 (Antioxidative Activities of Anthocyanins in Red Mustard Leaf Kimchi)

  • 최홍식
    • 한국식품영양과학회지
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    • 제32권6호
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    • pp.937-941
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    • 2003
  • 재래종 적갓(Brassica juncea czerniak et coss)을 주재료로 담금하여 발효시킨 적갓김치(red mustard leaf kimchi, RML kimchi)의 crude anthocyanin(CA) 및 refined anthocyanin(RA) 획분들에 대한 항산화성을 규명하였다. 발효된 적갓김치는 산도 0.72(%), pH 4.6 그리고 crude anthocyanin 함량(건조물기준)은 49.4 mg/g이었다. 동결건조 적갓김치로부터 얻어진 조색소용액 (crude pigment solution)을 계통 추출분획하여 증류수 획분, methanol 획분, TFA-methanol (CA) 획분을 얻었으며, CA 획분을 계속 분획하여 정제된 5개의 정제된 RA 획분(Fr.1, Fr.2, Fr.3, Fr,4, Fr.5)을 얻었다. 이들의 항산화 활성을 linoleic acid의 autoxidation system에서 peroxide formation 억제능과 DPPH($\alpha$, $\alpha$'-diphenyl-$\beta$-picrylhydrazyl)의 free radical 소거능에 대하여 실험하였다. 그 결과 조색소용액 분획물 중 CA 획분이 항산화능이 높았고, CA 획분으로부터 얻어진 RA 획분 중 Fr.3이 가장 높은 항산화성을 보였다. 특히 Fr.3은 적갓김치의 대표적인 anthocyanin이었고 항산화 활성은 $\alpha$-tocopherel보다는 낮았으나 그것에 접근하는 유사한 특성을 보였다. 그리고 그 활성은 지질의 과산화 억제능과 수소공여성에서 다같이 비슷한 수준의 항산화성을 보였다.

황금, 어성초를 배합한 익수영진고가미 한약재배합물의 베타글루칸, 진세노사이드 함량, 2,2-diphenyl-1-picrylhydrazyl Free Radical 소거 활성, 항염 활성 및 안전성 연구 (A Study on 𝛽-glucan, Ginsenoside Content, 2,2-diphenyl-1-picrylhydrazyl Free Radical Scavenging Activity, Anti-inflammatory Activity and Safety of Herbal Medicine Mix - Iksooyoungjingogami with Scutellariae Radix and Houttuynia cordata Thunb)

  • 김명훈;문양선;강상미;김형석;김선종;나창수
    • 한방재활의학과학회지
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    • 제32권2호
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    • pp.1-17
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    • 2022
  • Objectives This study was conducted to investigate the beta-glucan & ginsenoside content, antioxidant activity, anti-inflammatory effect and safety of herbal medicine mix. Methods The marker compounds contents, antioxidant activity and safety of herbal medicine mix were tested. The contents of beta-glucan and ginsenoside Rg3 were measured, the antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, anti-inflammatory and a safety test was conducted via single dose toxicity assessment. Results Analyzing the contents of marker compounds showed 362.3 mg/g of beta-glucan, and 0.4184 mg/g of ginsenoside Rg3. In the DPPH free radical scavenging activity, the IC50 of herbal medicine mix, was 0.146%. The scavenging activity of herbal medicine mix was 88.28% activity at 0.5% concentration, and 90.61% activity at 5% concentration. In the lipopolysaccharides (LPS) anti-inflammatory test, the herbal remix showed a significant decrease in tumor necrosis factor-alpha (TNF-𝛼) and interleukin-6 (IL-6) compared to the LPS-induced group. In the single dose toxicity test of herbal medicine mix, a dose of 2,000 mg/kg body weight (BW) was set at its highest capacity and observed after oral administration to female and male rats. No toxicological findings were recognized. It was observed that the resulting lethal dose can be set to 2,000 mg/kg BW or higher for both females and males. Conclusions The results of the experiment on herbal medicine mix showed that the marker compounds contents were beta-glucan and ginsenoside Rg3, that antioxidant activity was observed through the DPPH free radical scavenging activity, anti-inflammatory effect was observed through TNF-𝛼 and IL-6 measurement, and safety was confirmed through the single dose toxicity assessment.

Antioxidative Constituents of the Aerial Parts of Galium spurium

  • Yang, Seok-Won;Park, Sae-Rom;Ahn, Dal-Rae;Yang, Jae-Heon;Kim, Dae-Keun
    • Biomolecules & Therapeutics
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    • 제19권3호
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    • pp.336-341
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    • 2011
  • As part of an ongoing search for natural plants with antioxidant compounds by measuring the radical scavenging effect on 1,1-diphenyl- 2-picrylhydrazyl (DPPH), a total extract of the twigs of Galium spurium L. (Rubiaceae) was found to show potent antioxidant activity. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of nine compounds, asperulosidic acid methyl ester (1), asperuloside (2), caffeic acid (3), kaempferol-3-O-L-rhamnopyranoside (4), quercetin-3-O-[${\alpha}$-Lrhamnopyranosyl($1{\rightarrow}6$)-${\beta}$-D-glucopyranoside] (5), isorhamnetin-3-O-glucopyranoside (6), quercetin-3-O-${\alpha}$-L-rhamnopyranoside (7), kaempferol-3-O-[${\alpha}$-L-rhamnopyranosyl($1{\rightarrow}6$)-${\beta}$-D-glucopyranoside] (8), and quercetin (9). Their structures were elucidated by spectroscopic studies. Compounds 1, 3-8 were isolated for the first time from this plant. Among them, compounds 3 and 9 showed the significant radical scavenging effects on DPPH, and compounds 3 and 7 showed the potent riboflavin originated superoxide quenching activities.

가감형방지황탕 열수 추출물이 항산화 작용에 미치는 영향 (Antioxidant Property of the Gagam-Hyungbang-Gihwang-tang Using Biochemical Markers of Carcinogenesis)

  • 한진수;박성식
    • 대한한의학회지
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    • 제26권3호
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    • pp.204-214
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    • 2005
  • Objectives : The verity extract of the Gagam-Hyungbang-Gihwang-tang (GHG) was assessed to determine the mechanisms of its antioxidant activity. Methods : The fellowing effects were measured : GHG exhibited a concentration-treatment; scavenging ${\alpha},\;{\alpha}-diphenyl-\beta-picrylhydrazyl$ (DPPH) radical, linoleic acid oxidation in a thiocyanate assay system, and superoxide anion, hydroxyl radical-induced DNA nicking. We investigated mRNA levels such as superoxide-dismutase. Results : The GHG extract showed dose-dependent free radical scavenging activity, including DPPH radicals, hydroxyl radicals, and superoxide anion, using different systems. The GHG was also found to be effective in protecting plasmid DNA against the strand breakage induced by hydroxyl radicals in Fenton's reaction mixture. Furthermore, SOD-1 mRNA expression levels increased in tat hepatoma H4IIE cells Conclusions : We expect that GHG will to helpful to the development of antioxidant activity treatments.

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