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http://dx.doi.org/10.5352/JLS.2004.14.5.863

Antioxidative Effects of Pine (Pinus denstifora) Needle Extracts.  

유지현 (동아대학교 생명공학과)
차재영 (동아대학교 생명공학과)
정영기 (동의대학교 생명응용과학과)
정경태 (동의대학교 생명응용과학과)
조영수 (동아대학교 생명공학과)
Publication Information
Journal of Life Science / v.14, no.5, 2004 , pp. 863-867 More about this Journal
Abstract
Antioxidative activities of pine (Pinus denstifora) needle extracts were tested in vitro experimental models. The concentration of total polyphenolic compound of water extracts from pine needle was 1.61 %. In DPPH ($\alpha$, $\alpha-diphenyl-$\beta$-picrylhydrazyl) method, the electron donating activity of 0.1 % water extracts from pine needle was as high as BHT (0.05%, w/v). The antioxidative activity was measured by inhibition against lipid peroxidation of rat liver microsome, and this activity was shown in the following: 67.7% at 0.1% concentration >63.1% at 0.05% concentration > 28.2% at 0.01% concentration. In antioxidative activity determined by thiocyanate method against lipid peroxidation using linoleic acid, the antioxidative activities at all concentration of 0.01 %, 0.05% and 0.1 % were much higher than control during 7 days. In TBA method, the antioxidative activity was increased with increasing concentration until 6 days. These results support that water extracts from pine needle contain antioxidative compounds.
Keywords
Pind needle; polyphenolic compound; antioxidative activity; DPPH ($\alpha$, $\alpha-diphenyl-$\beta$-picrylhydrazyl);
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1 Bauernfeind, J. C. and D. M. Pinkert. 1970. Food processing with added ascorbic acid. Adr. in Food Res 18, 219   DOI
2 Bidlack, W. R. and A. L. Tappel. 1973. Damage to microsomal membrane by lipid peroxidation. Lipids 8, 177- 178   DOI   ScienceOn
3 Boo, Y. C., C. O. Jeon and J. Y. Oh. 1994. Isolation of 4-hydroxy-5-methyl-3[2H]-furanone from pine needles as an antioxidative principle. J. Korean Soc. Agric. Chem. Biotechnol. 37, 310-314   과학기술학회마을
4 Swain, T., W. E. Hillis and M. Oritega. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 83-88
5 Tappel, A. L. 1972. Vitamin E and free radical peroxidation of lipids. Ann. N. Y. Acad. Sci. 12, 203-208
6 Vergroeson, A. T. 1997. Physiological effects of dietary linoelic acid. Nutr. Rev. 35, 1-9
7 Wong, S. F., B. Holliwell, R. Richimond and W. R. Skowroneck. 1981. The role of superoxide and hydroxyl radical in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid. J. Inorganic Biochem. 14, 127-134   DOI   ScienceOn
8 Osawa, T. 1981. A novel type of antioxidant isolated from leaf was of Eucalyptus leaves. Agric. Biol. Chem. 45, 735- 739   DOI
9 Kim, S. M, Y. S. Cho and S. K. Sung. 1999. Effect of ethanol extracts in Pinus densiflora, Lithos permum erythrorhizon on the lipid oxidation of oil emulsion. J. Korean Soc. Food Sci. Nutr. 28, 984-989
10 Miura, K. and N. Nakatani. 1989. Antioxidative activity of flavonoid from Thyme. Agric. Biol. Chem. 53, 3043-3045   DOI
11 Ottolenghi, A. 1959. Interaction of ascorbic acid and mitochondrial lipids. Arch. Biochem. Biophys. 79, 355-461   DOI
12 Plaa, G. L. and H. Witschi. 1976. Chemicals, drugs and lipid peroxidation. Annu. Rev. Pharmacol. Toxicol. 16, 125- 131   DOI   ScienceOn
13 Chang, Y. S., U. Choi, D. H. Shin and J. I. Shin. 1992. Synergistic of Rhus javanica L. ethanol extract containing several synergist. Korean J. Food Sci. Technol. 24, 149-153
14 Saito, M. 1988. Interaction between lipid peroxide formation and nutritional status. J. Jpn. Soc. Nutr. Food Sci. 41, 343-349   DOI
15 Kang, Y. H., Y. K. Park, S. R. Oh and K. D. Moon. 1995. Studies on the physiological funtionality of pine needle and mugwort extracts. Korean J. Food Sci. Technol. 27, 978-984
16 Kim, E. J., S. W. Jung, K. P. Choi and S. S. Ham. 1998. Cytotoxic effect of the pine needle extracts. Korean J. Food. Sci. Technol. 30, 213-217   과학기술학회마을
17 Kim, H. J., J. Y. Cha, M. L. Choi and Y. S. Cho. 2000. Antioxidative activities by water extracts of Morus alba and Cudrania tricuspidata. J. Korean Soc. Agric. Chem. Biotechnol. 43, 148-152
18 Kim, J. D., T. H. Yoon, N. Choi, K. J. Im, J. S. Ju and S. Y. Lee. 1991. Effect of dietary supplementation with pine leaf on lipid parameters in rats. Kor. J. Gerontol. 1, 47-50
19 Brieskorn, C. H., A. Fuch, J. B. Bredenberg, J. D. Mcchensney and E. Wenkert. 1964. The structure of carnosol. J. Org. Chem. 29, 2293-2299   DOI
20 Cha, J. Y. and Y. S. Cho. 2001. Effect of stem bark extract from Morus alba and Cudrania tricuspidata on the lipid concentrations of lipid and tissue lipid peroxidation in the cholesterol-fed rats. Korean J. Food Sci. Thechnol. 33, 128- 134   과학기술학회마을
21 Choi, U., D. H. Shin, Y. S. Chang and J. I. Shin. 1992. Screening of natural antioxidant from plant and antioxidative effect. Korean J. Food Sci. Technol. 24, 142-148   과학기술학회마을
22 Chung, B. S. and M. K. Shin. 1990. The great dictionary of traditional and crude medicine. pp.3-152. YoungLim Press, Seoul
23 Esterbauer, H., J. Lang, S. Zadravec and T. F. Slater. 1987. Methods in Enzymology, Vol. 152, Academic Press Inc., New York
24 Annual report on the cause of death statistics. 1996. National Statistical Office, Republic of Korea
25 Foote, C. S. and R. W. Denny. 1968. Chemistry of singlet oxygen quenching by $\beta$-carotene. J. Am. Chem. Soc. 90, 6233-6239   DOI
26 Hwang, J. T., H. C. Kang, T. S. Kim and W. J. Prak. 1999. Lipid component and properties of grape seed oils. Korean J. Food. Nutr. 12, 150-155
27 Jun, B. S., J. Y. Cha and Y. S. Cho. 2001. Antioxidative activities of fruit extracts of Paulownia tomentosa Stued. Korean J. Postharvest Sci. Technol. 8, 231-238   과학기술학회마을