Natural Product Sciences

  • 제10권5호
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  • Pages.244-247
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  • 2004
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  • 1226-3907(pISSN)
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  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Inhibitory Effects of Methanol Extract, Phenolic Acids and Flavonoids from the Leaves of Eucalyptus darylmpleana against 1,1-Diphenyl-2-picrylhydrazyl Radical

  • 발행 : 2004.10.30
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초록

The inhibitory effects of the leaves of Eucalyptus darylmpleana (Myrtaceae) on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was examined. The scavenging effect of the ethyl acetate fraction of Eucalyptus darylmpleana leaves on DPPH radical was stronger than the other fractions, and further purified by silica gel and Sephadex LH-20 column chromatography. 3,4-Dihydroxybenzoic acid, gallic acid, quercetin, quercetin $3-O-{\alpha}-_L-rhamnoside$, quercetin $3-O-{\beta}-_D-glucoside$ and quercetin 3-O-rutinoside were isolated and elucidated by spectroscopic data. Among these components, gallic acid and quercetin $3-O-{\alpha}-_L-rhamnoside$ exhibited potent scavenging activities on DPPH radical with $IC_{50}$ values of 6.02 and $5.54\;{\mu}M$, respectively.

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참고문헌

  1. Cadahia, E., Conde, E., Garcia-VaIlejo, M.C., and Fermandez de Simon, B., Gel permeation chromatographic study of the molecular weight distribution of tannins in the wood, bark and leaves of EucaIyptus spp. Chromatographia 42, 95-100 (1996) https://doi.org/10.1007/BF02271062
  2. Conde, E., Cadahia, E., and Garcia-Vallejo, M.C., HPLC analysis of flavonoids and phenolic acids and aldehyde in Eucatyptus spp. Chromatographia 41, 657-660 (1995) https://doi.org/10.1007/BF02688103
  3. Ghisalberti, E.L., Skelton, B.W., and White, A.H., Structure study of torquatone, an acylphloroglucinol derivative from Eucatyptus species. Aust. J. Chem. 48, 1771-1774 (1995) https://doi.org/10.1071/CH9951771
  4. Hatano, T, Edamatsu, R., Hiramatsu, M, Mori, A., Fujita, Y, Yasuhara, T, Yoshida, T., and Okuda, T., Effects of theinteraction of tannins with co-existing substances. VI. Effects of tannins and related polyphenols and superoxide anionradical, and on 1,1-dipheny1-2-picry1hydrazy1 radical. Chem. Phann. Bull. 37, 2016-2021 (1989) https://doi.org/10.1248/cpb.37.2016
  5. Hillis, W.E. and Yazaki, Y, Wood polyphenols of Eucatyptus polyanthemos. Phytochemistry 12, 2969-2977 (1973) https://doi.org/10.1016/0031-9422(73)80517-5
  6. Hur, J.S., Ahn, S.Y, Koh, Y.J., and Lee, C.I., Antimicrobial properties of cold-tolerant Eucalyptus species against phytopathogenic fungi and food-bome bacterial pathogens. Plant Patho. J. 16, 286-289 (2000)
  7. Lee, I.K., Yun, B.S., Kim, J.R, Chung, S.H., Shim, G.S., and Yoo, I.D., Antioxidative compounds isolated from the stem bark of Eucatyptus gtobutus. Kor. J. Pharmacogn. 29, 163-168 (1998)
  8. Markham, K.R., Temai, B., Stanley, R., Geiger, H., and Mabry T.J., Carbon-13 nmr studies of flavonoids-III, Naturally occurring flavonoid glycosides and their acylated derivatives. Tetrahedron 34, 1389-1397 (1978) https://doi.org/10.1016/0040-4020(78)88336-7
  9. Mireku, E. and Wilkes, J., Production of phenols in the sapwood of Eucatyptus maculata after wounding and infection. Eur. J.For. Pathol. 18, 121-127 (1988) https://doi.org/10.1111/j.1439-0329.1988.tb00761.x
  10. Muller-Riebau, R, Berger, B., and Yegen, O., Chemical composition and fungitoxic prosperties to phytopathogenic fungi of essential oils of selectied aromatic plants growing wild in Turkey. J. Agric. Food Chem. 43, 2262-2266 (1995) https://doi.org/10.1021/jf00056a055
  11. Park, J.C., Young, H.S., Yu, Y.B., and Lee, S.H., Studies on the Chemical Components and Biological Activities of Edible Plants in Korea(l) : Phenolic compounds from the leaves of Cedrela sinensis A. Juss. Yakhak Hoeji 37, 306-310 (1993)
  12. Pouchert, C.J. and Behnke, J., The Aldnch Library of $^{13}C$ and $^1H$- NMR Spectra. Vol. 2, Aldhch, 1993, p.1 116
  13. Santos, G.G., Alves, J.C.N., Rodilla, J.M.L., Duarte, A.R, Lithgow, A.M., and Urones, J.G., Terpenoids and other constituents of Eucatyptus gtobutus. Phytochemistry 44, 1309-1312 (1997) https://doi.org/10.1016/S0031-9422(96)00680-2
  14. Seikel, M.K. and Hillis, W.E., Hydrolysable tannins of Eucatyptus detegatensis wood. Phytochemistry 9, 1115-1128 (1970) https://doi.org/10.1016/S0031-9422(00)85235-8
  15. Tumbull J.W., Future use of Eucalyptus: opportunities and problem, In: IUFR0 symposium on Intensive Forestry: the role of eucalytus. Vol. I., Durban, South Africa, 1991, PP. 2-29
  16. Yoshida, T., Maruyama, T, Nitta, A., and Okuda, T., Eucalbanine A, B and C, monomehc and dimeric hydrolyzable tannins from Eucatyptus alba. Chem. Pharm. Bull. 40, 1750-1754 (1992) https://doi.org/10.1248/cpb.40.1750
  17. Yun, B.S., Lee, I.K., Kim, J.R, Chung, S.H., Shim, G.S., and You, I.D., Lipid peroxidation inhibitory activity of some constituents isolated from the stem bark of Eucatyptus globus. Arch. Pharm. Res. 23, 147-150 (2000) https://doi.org/10.1007/BF02975503