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Inhibitory Effects of Methanol Extract, Phenolic Acids and Flavonoids from the Leaves of Eucalyptus darylmpleana against 1,1-Diphenyl-2-picrylhydrazyl Radical  

Park, Jong-Cheol (Department of Oriental Medicine Resources, Sunchon National University)
Park, Ju-Gwon (Department of Oriental Medicine Resources, Sunchon National University)
Hur, Jae-Seoun (Department of Environmental Education, Sunchon National University)
Choi, Myeong-Rak (Department of Biotechnology, Yosu National University)
Yoo, Eun-Jeong (Department of Biotechnology, Yosu National University)
Kim, Sung-Hwan (Kyongbuk Institute of Health and Environment)
Son, Jin-Chang (Kyongbuk Institute of Health and Environment)
Kim, Moon-Sung (Dong-A Pharm. Co.)
Publication Information
Natural Product Sciences / v.10, no.5, 2004 , pp. 244-247 More about this Journal
Abstract
The inhibitory effects of the leaves of Eucalyptus darylmpleana (Myrtaceae) on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was examined. The scavenging effect of the ethyl acetate fraction of Eucalyptus darylmpleana leaves on DPPH radical was stronger than the other fractions, and further purified by silica gel and Sephadex LH-20 column chromatography. 3,4-Dihydroxybenzoic acid, gallic acid, quercetin, quercetin $3-O-{\alpha}-_L-rhamnoside$, quercetin $3-O-{\beta}-_D-glucoside$ and quercetin 3-O-rutinoside were isolated and elucidated by spectroscopic data. Among these components, gallic acid and quercetin $3-O-{\alpha}-_L-rhamnoside$ exhibited potent scavenging activities on DPPH radical with $IC_{50}$ values of 6.02 and $5.54\;{\mu}M$, respectively.
Keywords
Eucalyptus darylmpleana; myrtaceae; DPPH; radical scavenging effect; gallic acid; quercetin $3-O-{\alpha}-_L-rhamnoside$;
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