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Screening of α-Amylase, α-Glucosidase and Lipase Inhibitory Activity with Gangwon-do Wild Plants Extracts (강원도 자생 산채 추출물의 α-Amylase, α-Glucosidase, Lipase 효소 저해활성 탐색)

  • Kim, Hee-Yeon;Lim, Sang-Hyun;Park, Yu-Hwa;Ham, Hun-Ju;Lee, Kwang-Jae;Park, Dong-Sik;Kim, Kyung-Hee;Kim, Song-Mun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.2
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    • pp.308-315
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    • 2011
  • We investigated ${\alpha}$-amylase, ${\alpha}$-glucosidase and lipase inhibitory activity of extracts collected from wild plants in Gangwon-do. 90 wild plants were collected and their water and ethanol extracts were obtained. Results of measuring ${\alpha}$-amylase inhibitory activity indicated more than 80% of activity inhibition in 10 mg/mL concentration for ethanol extracts of three plants and water extracts of two plants. For ${\alpha}$-glucosidase inhibitory activity, ethanol extracts of thirteen plants and water extracts of three plants showed more than 80% of activity inhibition in 10 mg/mL concentration. In the experiment of inhibiting lipase activity, ethanol extracts of seven plants and water extracts of one plants showed above 80% of activity inhibition in 10 mg/mL concentration. These results suggest that the selected extracts could be potentially used as a resource of bioactive materials for health functional foods.

Survey of Contaminants of 3-MCPD and 1,3-DCP in Soy Sauce Using GC/MSD (GC/MSD를 이용한 간장 중 3-MCPD와 1,3-DCP의 오염도 조사)

  • Kim Myung-Gill;Kim Young-Sug;Lee Myung-Jin;Kim Jae-Kwan;Kim Kyung-A;Park Eun-Mi;Ko Hoan-Uck;Son Jin-Seok
    • Journal of Food Hygiene and Safety
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    • v.21 no.3
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    • pp.153-158
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    • 2006
  • The contamination levels of 3-MCPD(3-Monochloropropane-1,2-diol) and 1,3-DCP(1,3-Dichloro-2-Propanol) in soy sauce, sauces was monitored. 105 samples were collected from June 2005 to August 2005 in Gyeonggi-do. 3-MCPD and 1,3-DCP were analyzed by GCMSD. As results, the detection range of 3-MCPD concentration was between 0.02 mg/kg and 0.27 mg/kg in soy sauce. However any samples were not detected with 1,3-DCP. The contaminated rates of 3-MCPD in soy sauce was 17.1%. Soy sauce is the representative vegetable protein hydrolyzed food. The reliable analytical method f3r the toxic 3-MCPD and 1,3-DCP in Soy sauce has been studied. The derivatization studies of 3-MCPD and 1,3-DCP were performed mainly as Silylation with BSA(N,O-Bis(trimethylsilyl)-acetamide). The retention times of derivative, BSA-MCPD, BSA-DCP were 11.30 and 10.12 minutes.

A Study on the Safety of Commercial Wet Tissues (유통 중인 물휴지의 안전성 조사 연구)

  • Bae, Ho-Jeong;Jung, Hong-Rae;Lee, So-Hyun;Lee, Seong-Bong;Song, Seo-Hyeon;Hong, Mi-Yeon;Kim, Beom-Ho;Park, Gwang-Hee;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.33 no.1
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    • pp.83-88
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    • 2018
  • The purpose of this study was to investigate and evaluate the safety of the wet tissues. In this study, we analyzed sterilizing preservatives and the presence of harmful substances in 62 wet tissue samples in the market. The contents of preservatives, formaldehyde and methanol were analyzed by HPLC and headspace-GC, respectively. Cetylpyridinium chloride was detected as 7-13 ppm in 5 samples. Sodium benzoate was detected in 46 samples ranging from 200 ppm to 3500 ppm, and 9 ppm of methylparahydroxy benzoate was detected in 1 sample. Propylparahydroxy benzoate was not detected in any samples. 5 ppm of methylchloroisothiazolinone and 140 ppm of methylisothiazolinone were detected in 1 sample. Formaldehyde was detected as $0.0069-1.796{\mu}g/g$ in 59 samples. Methanol was detected ranging from 2 ppm to 51 ppm in 22 samples, and 4 samples showed more than 20 ppm of the legal limit. The pH of the wet tissues was 4.0 to 8.2. Continuous investigation and monitoring are necessary to ensure safe distribution of products.

Breeding of Pleurotus eryngii with a high temperature tolerance trait (고온적응성 큰느타리(새송이)버섯 품종육성)

  • Im, Chak Han;Kim, Min-Keun;Kim, Kyung-Hee;Cho, Soo Jeong;Lee, Jong Jin;Joung, Wan-Kyu;Lee, Sang Dae;Choi, Young Jo;Ali, Asjad;Ryu, Jae-San
    • Journal of Mushroom
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    • v.12 no.3
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    • pp.187-192
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    • 2014
  • In order to breed a new P. eryngii cultivar with high temperature tolerance trait to cope with climate change, strains and cultivars were characterized at $20^{\circ}C$ that is $5^{\circ}C$ higher than normal condition followed by screening for the characteristics such as required days to harvest, quality and yield. Monokaryons from the selected strains were crossed. $Da-32{\times}KNR2322-15$ derived from the crosses between KNR2322 having characteristics of short growing day and Da($Ga5Na5-4{\times}KNR2312-7$) having charateristics of high guality and yield at $20^{\circ}C$, showed 14.9 days for harvest, 120.6 g yield, and 7.0 quality in the first trial. The strains were named as Taeyangsongi and cultivated on a large scale to compare with Kenneutari No. 2 at a mushroom farm. Yield of Taeyangsongi (109 g) was significantly different(P=0.001) from Kenneutari No.2. Quality of the new (6.6) and the reference cultivar (3.5) was also statistically different (P=0.001) The brightness of pileus of Taeyangsongi (59.5) was 10 points less than the reference cultivar due to which it has an inability to bear high temperature stress. Thus, PCR reactions with URP2 discriminated between Taeyansongi and reference cultivars (Keneutari No. 2 and Aeryni).

Comparison of Weather and Wave Data from Ocean Observation Buoys on the Southwestern Coast of Korea during Typhoon Muifa (태풍 무이파 내습시 서남해안 해양관측부이 기상파랑자료 비교 연구)

  • Yoon, Han-Sam;Kwon, Jun-Hyeok
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.15 no.3
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    • pp.170-176
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    • 2012
  • This paper analyzes the sea state and characteristics during the August 2011 passage of Typhoon Muifa based on data measured at four ocean weather/wave observation stations (buoys) located on the southwestern coast of Korea. When the typhoon arrived in the area approximately 230 km west of Mokpo at 9 PM on August 7, the decrease in air pressure led to increases in sea level of 25.64 cm at the Chilbal-do buoy, 16.43 cm at the Geomun-do buoy, and 9.60 cm at the Geoje-do buoy. The maximum wave height increased at the Geomun-do buoy about seven times faster than at the Chilbal-do buoy. The low water temperature at Chilbaldo during the typhoon passage probably reduced the wave energy. In the face of the oncoming typhoon, the southwest direction of the wind and waves may have been the result of external forces transporting seawater (energy) from the open sea toward the coast. The weather and ocean data from the Mara-do buoy were negatively correlated with those of Chilbal-do, whereas the data from Geomun-do had a positive correlation with those of Geoje-do.

Physicochemical Characteristics of Native Korean Allium wakegi Araki according to Different Seed Bulb Origins (종구산지에 따른 한국산 쪽파(Allium wakegi Araki)의 이화학적 특성)

  • Lee, Jeong-Ok;Kim, Kyung-Hee;Lee, Seong-A;Hwang, Hye-Rim;Kim, Mi-Seon;Choi, Jong-Jin;Yook, Hong-Sun
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.2
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    • pp.187-194
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    • 2009
  • The aim of this study was to investigate the physicochemical characteristics of native Korean Allium wakegi Araki samples, which were grown from seven local seed bulbs(Yesan, Muan, Anmyon-do, Deokjeok-do, Jeju-do, Yecheon, and China) to produce high quality native Korean Allium wakegi Araki. For the proximate composition of samples, moisture contents were in the range of 90.69~92.43%. The crude protein content of the Jeju-do sample was highest compared to the other samples. However, there were no significant differences in total sugar contents between samples. The seed bulb origin did not affect the hardness of the stem part, but was high for the leaves of the Yesan sample compared to the other samples. The results for anti-oxidative activity were as follows: Yesan(2.30 mg/mL) > China(2.51 mg/mL) > Muan (2.56 mg/mL) > Yecheon(2.74 mg/mL) > Jeju-do(2.85 mg/mL) > Anmyon-do(2.87 mg/mL) > Deokjeok-do(3.18 mg/mL). In terms of mineral and amino acid contents, the Yesan sample showed the highest levels, respectively, compared with the other samples. Food values such as contents of total phenolics and pyruvic acid were highest in the Jeju-do sample. These results show that the physicochemical characteristics of Allium wakegi Araki were significantly different according to different seed bulb origins.

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Characteristics of the Groundwater Quality for Paddy Fields in Korea (우리나라 논 관개용 지하수 수질 특성)

  • Kim, Jin-Ho;Cho, Kwang-Rae;Lim, Su-Jung;Lee, Kyung-Ja;Kyung, Gi-Chun;Eum, Mi-Jung;Kim, Hee-Kwon;Kim, Chan-Yong;Lee, Young-Han;Lee, Shin-Chan;Yun, Sun-Gang
    • Korean Journal of Environmental Agriculture
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    • v.22 no.4
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    • pp.241-245
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    • 2003
  • This study was carried out to evaluate the status of the groundwater quality for paddy fields irrigation in Korea. Water samples were collected at 130 sites throughout the country. Samples were collected at three seasons - April, July and October - in 2000. According to our survey, the groundwater was found to be suitable for irrigation purpose. Average EC was shown 0.286 dS/m. Nitrate-nitrogen and $Cl^-$ concentration was 5.6 mg/L, 32.95 mg/L which satisfied the Korean Standards for Irrigation Water. Nitrate-nitrogen concentration in each province was shown as following orders: Jeju (11.17 mg/L) > Chungnam (8.16 mg/L) > Gyeongbuk (6.64 mg/L) > Gyounggi (5.91 mg/L) > Chungnam (4.95 mg/L) > Gyeongnam (3.91 mg/L) > Jeonbuk (3.50 mg/L) > Jeonnam (3.27 mg/L) > Gangwon (2.91 mg/L). The concentration by sampling seasons were October (6.62 mg/L) > July (5.88 mg/L) > April (4.78 mg/L). As the soil of Jeju Province is usually derived from volcanic ash soils mainly used for upland drops, it may influence the nitrate-nitrogen concentration of groundwater. The amount of rainfall also influence the water quality. But the $COD_{Cr}$ were shown April (3.17 mg/L) > July (2.91 mg/L) > October (2.40 mg/L), it is highly related in the basal dose of organic matter fertilizers. This study demonstrated that groundwater quality was suitable for irrigation, but continuous monitoring is recommended for agricultural policy and developing OECD agricultural environment indicators.

STUDY ON THE MORPHOLOGICAL VARIATIONS OF MUSSEL MYTILUS CORUSCUS GOULD (담치의 형태변이에 관한 연구)

  • YOO Sung Kyoo;KANG Yong-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.7 no.2
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    • pp.87-90
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    • 1974
  • The followings are the results of study about morphological variation of mussel, Mytilus coruscus collected respectively from Sanyang-myeon Tongyeong-goon Gyeongnam on 13th January, Yeonwha-do Yokji-myeon Tongyeong-goon Gyeongnam on 15th May and down below the Yeong-do Bridge Busan on 20th August, 1964. The biggest mussel of the above three areas have been from Yeong-do Bridge, whose shell height is 164.1 mm, shell length 77.8mm, shell breadth 52.2mm, total weight 291,9g and shell length is as follows : from Yeong-do Bridge L=0.4954H + 1.9516, from Sanyang-myeon L=0.3718H + 14.145, from Yeonwha-do L=0.4074H + 9,6610 The relationship between shell height and shell breadth is as follows : from Yeong-do Bridge B=0.3426H + 0.2052, from Sanyang-myeon B=0.3084H + 3.6183, from Yeonwha-do B=0.3507H + 0.8028 In view of the above relationship, it is concluded that the slope value of mussel, nearer to the inshore from the off-sea, is similar to that of M. edulis, from which are can presume that M. coruscus could be changed in shell form according to its environment. Growth curve between total weight and shell height is as follows : from Yeong-do Bridge W=0. 00020469 $$H^{2.79745}$ from Sanyang-myeon W=0.00061512$H^{2.53708}$ from Yeonwha-do W=0.00016965$H^{2.83960}$

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Breeding of King Oyster Mushroom, Pleurotus eryngii with a High Yield and Earliness of Harvest Trait and Its Sensory Test (큰느타리(새송이)버섯 다수확 속성 품종 육종 및 관능평가)

  • Im, Chak Han;Kim, Min-Keun;Kim, Kyung-Hee;Kim, Sun Young;Lee, Seong Tae;Heo, Jae Young;Kwon, Jin-Hyeuk;Kim, Dong Sung;Ryu, Jae-San
    • The Korean Journal of Mycology
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    • v.41 no.2
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    • pp.91-96
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    • 2013
  • Two strains Pleurotus eryngii 'Aeryni' and 'Na' carrying superior traits of a pileus and a earliness of harvest were selected to improve previously bred strains by single crosses. New hybrid, Aeryni 3 (Aeryni10 ${\times}$ Na5) showed superiority to other hybrids in yield, fruit body shape and days for harvest. The new strain, Aeryni 3 was harvested earlier than Keunneutari No. 2 by 2~3 days, and yielded 110.5 g/bottle (850 mL) which was 108% of that of Keunneutari No. 2. The ratio of diameter of pileus and stipe was 1.8 indicating that new stain will be likely low damage rate of fruit body during a distribution, and that was better than 2.1 of Keunneutari No. 2. A sensory test of taste of the new strain showed that 84.7% of evaluation panels selected "very good" while that of Keunneutari No. 2 was 55.5%. In purchasing intent test, 86.9% of panels will buy the new stain whereas 46.8% will buy Keunneutari No. 2 implicating that the new strain will likely be more marketability than previously bred strain.

Study of necessity of teenagers' drinking, mouth knowledge estimation by smoking and dental health education (청소년의 음주·흡연에 따른 구강지식평가 및 구강보건교육의 필요성에 관한 연구)

  • Choi, Yun-Hwa;Jee, Yun-Jeong
    • Journal of Korean society of Dental Hygiene
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    • v.8 no.3
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    • pp.85-98
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    • 2008
  • This study is to enforce to ready basic data of effective dental education program and no smoking and no drinking program for high school students who do smoking and drinking, executed investigation by own recording way using smoking and questionnaire consisted of actual conditions and caries prevention awareness and dental health knowledge degree of drinking to some man high school. Analyzes result that total 487 people respond in SPSS WIN 13.0 programs and got following sequence. Result of this study is as following. 1. Mans high school students had more students who do not than a drinking student and main current received most soju. Third-year student appeared by thing which receive a lot of soju than 1 or 2 class student but different other difference was not seen by class. 2. Mans high school students had more students who do not than a smoking student and a student who smoke more than 3 years was the most. I can know was old since third-year student smokes than 1 or 2 class student. 3. It was not difference that keep in mind by drinking, smoking availability although there were many students who recognize that all of the on-time dental medical examination, sugar intake limitation, fluorine inclusion toothpaste, teeth fluorine application are seldom effective on caries prevention. 4. Recognized that right brushing is effective relatively on caries prevention. I was shown difference that recognized that righter brushing is effective on bad tooth courtesy call than a student who a student who do not smoke does, and keeps in mind according to smoking availability ($x^2$=6.78, p<0.05). 5. I knew best about question of 'A caries may not treat if is painless' among that question ask knowledge for a caries, and appeared by thing which do not know best about question of 'Know that method that supply fluorine in water'. 6. A student who do not drink general dental know-how for a caries was high knowledge degree for a caries than a drunk student but it was no difference that keep in mind by drinking availability. Also, a student who do not smoke by smoking availability was high knowledge degree for a caries than a done student but difference that keep in mind statistically was not seen. 7. It was no difference that keep in mind by drinking availability, but a student who do not smoke by smoking availability was high relation knowledge degree with food and a caries than a smoked student, and I was shown difference that keep in mind according to smoking availability(t=-2.03, p<0.05). 8. Awareness and synthetic estimation degree of knowledge degree a student who do not smoke than a smoked student mouth knowledge degree high, and I was shown difference that keep in mind according to smoking availability(t=-2.03, p<0.05). When see as result such as singularity, family, school, all social institutions may have to help by national business coming and try so that can become national business based on special know-how for development of effective and epoch-making education program as quickly as possible.

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