Journal of Korean Society of Environmental Engineers
/
v.30
no.9
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pp.939-947
/
2008
PCR-DGGE method was applied to analyze changes of microbial community in simultaneous nitrification and denitrification (SND) bioreactor with various DO concentrations. In the analysis of eubacterial community, band profiles of DGGE were similar with 2 or 1 mg/L DO concentrations in the reactor. Experimental results led to 16 different bacteria being identified, including 5 dominant strains(3 strains of Uncultured Bacterium, 1 strains of Bacillus, 1 strains of Uncultured Bacteroidetes). DGGE results at 0.5 mg/L DO concentration led to 12 strains being identified, including 7 dominant strains(5 strains of Uncultured Bacterium, 2 strains of Zoogloea sp.). DGGE results at 0.1 mg/L DO concentration led to 11 strains being identified, including 3 dominant strains(1 strains of Uncultured Bacterium, 2 strains of Zoogloea sp.). In DGGE band profiles of $\beta$-AOB($\beta$-Ammonia Oxidizing Bacteria), only one band was observed. This band had 97% similarity with Nitrosomonas sp. done DNB Y20. This band was clearly observed at the 2, 1 and 0.5 mg/L DO concentrations, while the brightness of the band at 0.1 mg/L DO concentration was mostly dimmed. In DGGE band profiles of denitrification process, 5 bands(3 strains of Uncultured organism containing nirS, 2 strains of Uncultured organism containing nirK) were observed. Among those bands, the brightness of one band was gradually increased at the lower DO concentrations. This band has 86% identity with Uncultured organism clone eS1 cd1 nirS gene, partial cds. Based on this result, it could be concluded that Uncultured organism clone eS1 cd1 nirS gene, partial cds is a predominant microorganism in the denitrification process.
The relationships between wave and wind around the Korean Peninsula have been analyzed with the data from the buoys moored at five stations (Dugjug-do, Chilbal-do, Geomoon -do, Geoje-do, Donghae) by Korea Meteorological Administration. Generally, the relationship between wave and wind is the highest at the stations in the West Sea and the lowest at the stations in the South Sea, and the middle at the station in the East Sea. The characteristics shown at each station are as follows. Highest wave is developed at Chilbal-do with strong northwesterly wind in winter because the sea is opened in the wind direction and wave is amplified by shoaling effect. At Chilbal-do, wave directions coincide with wind directions relatively well. On the other hand, waves are not fully developed at Dugjug-do in winter due to limited fetch since the sea is blocked by Hwanghae-do in the northwest direction. The limitation in fetch is more serious at the stations in the South Sea. In the South Sea, the direction of dominant northerly wind is blocked by land so that wave heights are small even with very strong northerly wind. In the South Sea, whatever wind direction is, waves dominantly come in the direction from the East China Sea, which are from the south at Geomoon-do and the southwest at Geoje-do. At these directions, waves are coming even with weak wind. At the station in the East Sea, waves are highly developed due to vast area, but not so high as in Chilbal-do because wind and wave directions do not coincide in many cases. As shown, wind direction is important in the wave development as well as wind speed. The reason is that the fetch is determined by wind direction. In the case of long-lasted wind with fixed direction at Chilbal-do and Dugjug-do, wave directions are well coincident with wind directions and wave heights increase with response time, which is the duration between the highest wind and wave. However, in the case of disagreement between wind and wave directions at the station in the East Sea, wave heights do not increase as highly as at Chilbal-do and Dugjug-do in spite of strong wind and longer response time. The results show us that waves are highly developed with strong wind, long fetch, and long duration, and also show that wave development ratios are different at different stations due to environmental factors such as the direction towards sea or land, bottom topography, and the scales of adjacent seas.
Legionnaires' disease (LD) is a severe and potentially fatal pneumonia caused by colonization of human-made water system and subsequent aerosolization and inhalation of Legionella bacteria. A total of 147 Legionella strains was isolated from environmental water sources from public facilities in Gyeonggi-do, South Korea. The distribution of Legionella isolates was investigated according to facility type, and sample type. L. pneumophila was distributed broadly throughout Gyeonggi-do, accounting for 85.7% of the isolates, and L. pneumophila serogroup (sg) 1 predominated in all of the public facilities. L. wadsworthii predominated among non-L. pneumophila species. We performed comparative analyses of L. pneumophila sg 1 isolated from environment water of public facilities in Gyeonggi-do by pulsed field gel electrophoresis (PFGE) and sequence-based typing (SBT). Thirty-two isolates were classified into 22 types by PFGE and 9 sequence types (STs) by SBT and categorized into 3 groups. ST1 was the most prevalent sequence type and two STs obtained in this study had unique allelic profiles. The use of SBT data from different countries for epidemiology study of LD constitutes a technically uncomplicated and relatively easy method for strain subtyping, especially compared to other contemporary techniques.
Kim, Woongsoo;Song, Ilseok;Shin, Jonghyun;Oh, Cheonhwan;Kim, Eunah;Kim, Keugtae;Kim, Hyunja;Kim, Jongsu;Choi, Yunho
Journal of Environmental Health Sciences
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v.43
no.3
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pp.233-239
/
2017
Objectives: The pollution status of heavy metals within the soil was investigated with an aim to establishing a sustainable soil environment within parks and amusement facilities installed in urban areas of Gyeonggi-do Province. Methods: As sampling sites, 14 locations were selected from a city with a number of factories near a residential area, a residential area, and a children's park in a city with mixed green areas. Seven kinds of heavy metals, including Cd, Pb, and Hg, and the pH of soil were analyzed three times by inductively coupled plasma optical emission spectroscopy (ICP-OES) and atomic absorption spectrometer (AAS). Results: In this study, the pH of the samples from the residential park and industrial park showed 5.7-6.5 and 5.9-7.0, respectively. The overall mean concentration (mg/kg) of heavy metals was Zn (132.8), Ni (73.0), Cu (47.4), Pb (35.9), As (4.84), Cd (0.39), and Hg (0.07), indicating that these concentrations of heavy metals were lower than those for the area 1 standard of soil pollution concern criteria. In addition, the sampling sites in the residential area and the industrial area also showed the same tendency for concentration distribution. Conclusions: We found that the soil pollution class (SPC) of some spots were over 200, which are third and fourth classes. In order to manage a sustainable soil environment in a city park, it is suggested that local governments, the management bodies for these parks, need to manage, supervise, and investigate soil pollution and quickly replace contaminated soil.
Objectives: A lake is a place used by many people, and compared to rivers it is easy for them to become polluted. The water quality in lakes and reservoirs has been worsening recently. The purpose of this study is to evaluate the water pollution characteristics of major lakes in northern Gyeonggi-do Province. Methods: Six lakes were selected as major lakes and were evaluated in terms of water pollution characteristics and eutrophication (as defined by results for $COD_{Mn}$, TOC, SS, Chl-a, T-N and T-P) over one year (from December 2016 through November 2017) in northern Gyeonggi-do Province. Results: The annual average $COD_{Mn}$ was found to be 3.1 mg/L in Onam, 3.6 mg/L in Sanjeong, 4.7 mg/L in Gisan, 4.8 mg/L in Ilsan, and 6.1 mg/L in Jangja. The results of the Korean trophic state index ($TSI_{KO}$ ) value indicated a eutrophic state ($TSI_{KO}$ 59.0) in Jangja lake. The other lakes were classified as being in a mesotrophic state ($TSI_{KO}$ of 38.1 in Sanjeong, 40.2 in Ilsan, 41.9 in Onam, 46.1 in Gisan, and 47.8 in Gomo). Conclusions: Ilsan Lake's water quality is being well maintained. Sanjeong, Onam, and Gisan are appropriate for use as agricultural water. Jangja lake requires efforts for water quality improvement and to prevent the inflow of non-point pollutant sources.
Objectives: This study was performed to determine environmental hazard factors and provide more eco-friendly child activity spaces within children's playgrounds installed in Gyeonggi-do Province. Methods: Basic (XRF) and precise (ICP, UV) inspections were conducted. The test items examined were heavy metals and parasite eggs. As a sampling point, painted finish materials (180), synthetic rubber (50), and sand (50) were selected. Results: The total excess rate of heavy metals in the XRF was found to be 7.4% (17/230 points). In a comparison between the basic and precise inspections with 17 excess points, the concentration deviation between the two tests was found to be from 0.01 to 7.7 times, resulting in a large difference. Furthermore, all the excess samples were dual samples. However, the contribution rates of Pb and $Cr^{6+}$ to combined concentration were found to be 85.1 and 14.9% for basic inspection and 91.9 and 8.1% for precise inspections, so there is a similar tendency between the two tests. The excess rate of parasite eggs in sand was expressed at 6%. The excess rate of heavy metals in synthetic rubber was found to be 0%. Conclusion: The reliability of the XRF is low. However, considering the contribution rate of Pb and $Cr^{6+}$ between the two tests, it is likely to be applicable for screening. Dual samples provided high concentrations and excess samples and care should be taken when managing them.
MPEG-I is actively working on standardization on the coding of immersive video which provides up to 6 degree of freedom (6DoF) in terms of viewpoint. 3DoF+ video, which provides motion parallax to omnidirectional view of 360 video, renders a view at any desired viewpoint using multiple view videos acquisitioned in a limited 3D space covered with upper body motion at a fixed position. The MPEG-I visual group is developing a test model called TMIV (Test Model for Immersive Video) in the process of development of the standard for 3DoF+ video coding. In the TMIV, the redundancy between a set of input view videos is removed, and several atlases are generated by packing patches including the remaining texture and depth regions into frames as compact as possible, and coded. This paper presents an atlas generation method that removes small-sized blocks in the atlas for more efficient 3DoF+ video coding. The proposed method shows a performance improvement of BD-rate bit savings of 0.7% and 1.4%, respectively, in natural and graphic sequences compared to TMIV.
Kenaf (Hibiscus cannabinus L.) was recognized as a potential source of forage. But the domestic cultivation techniques are not set standards yet. So we tested the basic culture techniques during 2012~2013 for getting a high yield and good grade forage production system. The best seeding method for mechanized planting (corn planter used) was hill seeding with $20{\times}20cm$ seeding distance. When we treated hill seeding with $20{\times}20cm$, the yield what we could get was 13,641 kg/10a and it was 32% more than that of conventional practice hill seeding with $20{\times}30cm$ seeding distance. The proper seeding date for getting high yield was May 1. In May 1, the yield per 10a was reached 13,423 kg, and it was 30% more than that of seeding at May 30. More over the crude protein content which was important factor for determinating forage nutritive value was 12.7% and it was higher 1.8% (relatively 16.5% high) than that of May 30 seeding. The most effective herbicide for kenaf was Fluazipof-p-butyl. It's herbicidal rate was 97% and phyto-toxicity was less than 5%. Regional adaptability for Jeollabuk-Do including Imsil Gun, Kochang Gun and Sunchang Gun were identified that the stable cultivation were possible in these area with average yield 12,400 kg/10a and it was about 1.7 times as compared to corn harvest.
The monitoring of pesticide residues was performed on 33 fruit commodities collected in Gyeonggi-do, Korea from 2006 to 2010. Pesticide residues were detected in 431 samples (16.8%) of total 2,558 samples and violated in 12 samples (0.5%). Annual detection rate showed 6.9%~19.4% with the rate of violation of 0.3%~0.9%. Twenty three samples (69.7%) of 33 commodities were detected and 4 samples (12.1%) were violated. Eight pesticides (EPN, dicofol, carbaryl, procymidone, methidathion, prothiofos, fenitrothion and phenthoate) were violated and 62 pesticides were detected. Chloropyrifos was detected most frequently. The rate of detection and violation of citrus fruits in fruits showed the highest level. Organophosphorus pesticides (35%) and insecticide (57%) were detected most frequently. The hazard index (%ADI) of chronic dietary risk assessment by deterministic approach showed that the lower limit value and upper limit value for the whole population were 0.0000~0.7526 and 0.0000~1.3237 respectively. For the only consumer group, the lower limit value and upper limit value were 0.0006~9.7801 and 0.0058~15.9258 respectively. Therefore the hazard index for the whole population and the only consumer group were evaluated as a safe level.
This study investigated the hydraulic impact scope and dissolved oxygen (DO) concentration distribution by the micro-bubble aeration in the Juksan Lake located in Asan city in Chungcheongnam-do province. A tracing experiment for hydraulic impact scope was used which constituted a 20% rhodamine solution. A 160 m-guideline was installed in the horizontal direction of the micro-bubble jet flow and the rhodamine concentration, water temperature, and DO concentration were measured at depths of 1 m, 2 m, and 3 m at intervals of 10 m. In the Juksan Lake, the effective range of jet flow discharged by the micro-bubble generator was about 40 m, and after then the jet plume moved up to 80 m to 120 m through the advection and diffusion processes of ambient water. DO concentration in the lake was maintained at 7.4-12.6 mg/L during tracking experiment. The DO of the lake sediments improved from 0.2 mg/L to 8.0 mg/L after applying micro-bubble aeration. In conclusion, the micro-bubble aeration can be an effective technology for the management and improvement of water quality in an agricultural reservoir.
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