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http://dx.doi.org/10.7845/kjm.2017.7027

Distribution of Legionella species from water systems and genetic diversity of L. pneumophila serogroup 1 in Gyeonggi-do  

Lee, Hyun-Kyung (Microbiology Team, Gyeonggi-do Institute of Health and Environment)
Park, Yong-Bae (Microbiology Team, Gyeonggi-do Institute of Health and Environment)
Hwang, Sun-Il (Microbiology Team, Gyeonggi-do Institute of Health and Environment)
Kim, Young-Su (Microbiology Team, Gyeonggi-do Institute of Health and Environment)
Park, Nan-Joo (Microbiology Team, Gyeonggi-do Institute of Health and Environment)
Park, Kwang-Hee (Microbiology Team, Gyeonggi-do Institute of Health and Environment)
Yoon, Mi-Hye (Microbiology Team, Gyeonggi-do Institute of Health and Environment)
Publication Information
Korean Journal of Microbiology / v.53, no.3, 2017 , pp. 156-162 More about this Journal
Abstract
Legionnaires' disease (LD) is a severe and potentially fatal pneumonia caused by colonization of human-made water system and subsequent aerosolization and inhalation of Legionella bacteria. A total of 147 Legionella strains was isolated from environmental water sources from public facilities in Gyeonggi-do, South Korea. The distribution of Legionella isolates was investigated according to facility type, and sample type. L. pneumophila was distributed broadly throughout Gyeonggi-do, accounting for 85.7% of the isolates, and L. pneumophila serogroup (sg) 1 predominated in all of the public facilities. L. wadsworthii predominated among non-L. pneumophila species. We performed comparative analyses of L. pneumophila sg 1 isolated from environment water of public facilities in Gyeonggi-do by pulsed field gel electrophoresis (PFGE) and sequence-based typing (SBT). Thirty-two isolates were classified into 22 types by PFGE and 9 sequence types (STs) by SBT and categorized into 3 groups. ST1 was the most prevalent sequence type and two STs obtained in this study had unique allelic profiles. The use of SBT data from different countries for epidemiology study of LD constitutes a technically uncomplicated and relatively easy method for strain subtyping, especially compared to other contemporary techniques.
Keywords
Legionella spp.; Legionella pneumophila; Legionnaires' disease (LD); PFGE; SBT;
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