• Title/Summary/Keyword: DNP3

Search Result 156, Processing Time 0.031 seconds

Eosinophil and IgE responses of IL-5 transgenic mice experimentally infected with Nippostrongylus brasiliensis

  • Chai, Jong-Yil;Shin, Eun-Hee;Takatsu, Kiyoshi;Matsumoto, Naoki;Kojima, Somei
    • Parasites, Hosts and Diseases
    • /
    • v.37 no.2
    • /
    • pp.93-99
    • /
    • 1999
  • Eosinohil and IgE responses of interleukin IL(-5 transgenic and normal C3H/HeN mice were studied after experimental infection with Nippostrongylus brasiliensis 9Nb). Intestinal worms were recovered at day 5 post-infection (PI), and numbers of total white blood cells (WBC) and eosinophils, and total serum IgE and anti-hapten (dinitrophenyl)(DNP) specific IgE titers, were measured at days 0,14 and PI. IL-5 mice appeared resistant to Nb infection showing a significantly ower worm recovery rate than normal mice (P<0.05). Total WBC and eosinophil counts (/mm3) were significantly increased in Nb infected normal mice (p<0.05), but unchanged (total WBC) or decreased (eosinophils) in IL-5 mice at day 21 PI. The total serum IgE level remarkably increased in normal mice, but only a little in IL-5 mice at days 14 and 21 PI. Priming with DNP brought about more remarkable increases of the total and anti-DNP specific IgE in normal mice than in IL-5 mice. The results show that IL-5 mice are resistant to Nb infection, and that eosinophil and IgE responses in these mice are not augmented by N infection.

  • PDF

Suppressive Effects of Fucoxanthin on Degranulation in IgE-antigen complex-stimulated RBL-2H3 Cells

  • NamKoong, Seung;Joo, Hae-Mi;Jang, Seon-A;Kim, Ye-Jin;Kim, Tae-Seong;Sohn, Eun-Hwa
    • Korean Journal of Plant Resources
    • /
    • v.25 no.3
    • /
    • pp.339-345
    • /
    • 2012
  • The marine carotenoid fucoxanthin can be found in marine brown seaweeds, macroalgae, diatoms, and microalgae, and has remarkable biological properties. Numerous studies have shown that fucoxanthin has considerable potential and promising applications in human health, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We here investigated the mechanisms by anti-allergic activity of fucoxanthin fraction from Eisenia bicyclis in immunoglobulin E-antigen complex (IgE/DNP-BSA)-stimulated RBL-2H3 mast cells. This study we found that the fucoxanthin inhibits the release of ${\beta}$-hexosaminidase and suppressed not only transcriptional activation of NF-${\kappa}B$, but also phosphorylation of ERK and JNK in IgE/DNP-BSA-treated RBL-2H3 cells. Fucoxanthin may be useful for preventing allergic diseases, including asthma and atopic dermatitis.

The Antiallergic and Antioxidative Effects of Chaenomeles Sinensis (CS) in RBL 2H3 Cells (목과(木瓜) 추출물이 항알레르기 및 항산화 작용에 미치는 영향)

  • Lee, Seung-Yeon
    • The Journal of Pediatrics of Korean Medicine
    • /
    • v.24 no.2
    • /
    • pp.126-136
    • /
    • 2010
  • Objectives The purpose of this study was to investigate the antiallergic and antioxidative effects of Chaenomeles Sinensis (CS). Methods CS pretreatments inhibited anti-DNP IgE in RBL-2H3 mast cells for an hour. we measured cell viability, $\beta$-hexosaminidase release, IL-4, TNF-$\alpha$ secretion, and IL-4, TNF-$\alpha$ mRNA expression CS pretreatments inhibited DNP-HSA($10\;{\mu}g/ml$) for ten minutes, we measured Dicholrodihydrofluorescein(DCF) and DPPH radical-scavenging activity in 0.5 mM 1,1-diphenyl-2-picrydrazyl(DPPH) radical solution, 0.1ml, 99% ethanol 0.8ml, and CS 0.1 ml mixed solution. Results 0, 1, 2, 3, 4, 5 mg/ml CS treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF-$\alpha$ secretion, CS treatments also decreased IL-4, TNF-$\alpha$ mRNA expression in RBL-2H3 cells. CS treatments inhibited reactive oxygen species(ROS) and DPPH radical-scavenging activity. Conclusions These results suggest that CS may be useful for the prevention or treatment of allergic disease.

A Study of Accumulated Ecosystem Carbon in Mt. Deogyusan, Korea (덕유산의 생태계 탄소축적량 산정에 관한 연구)

  • Jeong, Seok-hee;Eom, Ji-young;Jang, Ji-hye;Lee, Jae-ho;Cho, Koo-hyun;Lee, Jae-seok
    • Korean Journal of Environmental Biology
    • /
    • v.33 no.4
    • /
    • pp.459-467
    • /
    • 2015
  • Understanding of a carbon storage in a regional scale ecosystem is a very important data for predicting change of global carbon cycle. Therefore, the real data collected in the various ecosystems are a very useful for enhancing accuracy of model prediction. We tried to estimate total accumulated ecosystem carbon in Deogyusan National Park (DNP) with naturally well preserved ecosystem. In DNP, vegetations were classified to four main communities with Quercus mongolica community (12,636.9 ha, 54.8%), Quercus variabilis community (2,987.0 ha, 13.0%), Pinus densiflora community (5,758.0 ha, 25.0%), and Quercus serrata community (402.9 ha,1.7%). Biomass and soil carbons were estimated by the biomass allometric equations based on the DBH and carbon contents of litter and soil (0~30 cm) layers collected in 3 plots ($30cm{\times}30cm$) in each community. The biomass and soil carbons were shown as high value as 1,759,000 tC and 7,776,000 tC, respectively, in Quercus mongolia community in DNP area. In Quercus mongolica, Quercus variabilis, Quercus serrata, Pinus densiflora communities, the accumulated ecosystem carbon were shown 9,536,000 tC, 1,405,000 tC, 147,000 tC, 346,000 tC, respectively. Also, the total ecosystem carbon was estimated with 11,434,000 tC in DNP.

Effects of Perilla frutescens Extract on Anti-allergic Reactions in a Mouse Model (소엽 추출물이 마우스모델에서 항알레르기 반응에 미치는 영향)

  • Ko, Jung-A;Lim, Hun-Sun;Kim, Gun-Hee;Park, Ji-Yong;Han, Chan-Kyu;Park, Hyun-Jin
    • Korean Journal of Food Science and Technology
    • /
    • v.42 no.4
    • /
    • pp.488-493
    • /
    • 2010
  • We investigated the effect of Perilla frutescens (PF) ethanol extract powder (PF-E30) on the local allergic reaction activated by anti-DNP IgE and the mast cell-mediated immediate-type allergic reactions induced by compound 48/80 in a mouse model. One gram of PF powder extracted with 30% ethanol at $80^{\circ}C$ contained 12.3 mg of rosmarinic acid. Oral administration of PF-E30 (0.1 to 0.5 mg/kg body weight) significantly reduced plasma histamine levels and inhibited histamine release from peritoneal mast cells in mice activated by compound 48/80 or anti-DNP IgE. Moreover PF-E30 dose-dependently inhibited the production of antigen-induced IgE. These results indicate that the PF ethanol extract inhibits mast cell-mediated immediate-type allergic reactions in vivo and in vitro.

Monitoring system design&Implementation using wireless solution (무선 통신을 사용한 모니터링 시스템의 설계 및 구현)

  • Jeon Yoon-Ho;Woo Jong-Hyun;Kim Young-Ryun;Kim Hee-Dong
    • 한국정보통신설비학회:학술대회논문집
    • /
    • 2002.08a
    • /
    • pp.39-42
    • /
    • 2002
  • RS-232C는 정보기기들을 연결하는 직렬통신 표준으로, 간단한 구조로 인해, 복잡한 요구 조건이 없는 상용 기기들의 제어 및 감시용으로 사용되고 있다. 최근 무선동신기술이 발전함에 따라, 저가격의 무선통신 기술을 활용하여 무선 RS-232C에 대한 요구가 늘어나고 있다. 무선 RS-232C로 기존 유선의 RS-232C를 대체함으로서, 기존 시스템의 변경없이 편리한 접속기능을 대체한 수 있을 것이다. 본 논문에서는 유선 RS-232C를 대체하는 무선 RS-232C 모듈을 채용하여, 전력제어에 사용하는 배전자동화 장치의 관리를 간편하게 하는 시스템의 구현에 대해서 다루고 있다. 배전 자동화시스템은 일종의 SCADA시스템의 일부로서, 통신 프로토콜로서 산업용 DNP3.0을 사용하고 있다. 무선 RS-232C 링크의 신뢰성을 확보하기 위하여, 무선 구간에 DNP3.0을 채택하였으며, 무선 통신방식으로는 무선 RF, 블루투스(Bluetooth), 무선랜(wireless LAN)등 3가지 방식을 채용하여, 설계, 구현내용을 기술하였다.

  • PDF

Inhibitory Effect of Rhododendron Mucronulatum Root Extract on Allergic Inflammation (진달래 뿌리 추출물의 알레르기 염증 억제 효과)

  • Jang, Si Sung;Lee, DaeJoong;Song, Jihoon;Park, Do Hwi;Jeon, Chan Yong;Hwang, Gwi Seo
    • The Journal of Internal Korean Medicine
    • /
    • v.43 no.1
    • /
    • pp.68-78
    • /
    • 2022
  • Objective: In this study, we investigated the protective effect of rhododendron mucronulatum extract (RME) on allergic reactions and inflammation. Methods: The effect of RME was determined using ELISA and RT-PCR in RBL-2H3 mast cells and RAW 264.7 macrophage cells. We determined cell viability, β-hexosaminidase release, and the synthesis of IL-4 and TNF-α in RBL-2H3 cells. In addition, we determined NO from RAW 264.7 and the gene expression of IL-1β, iNOS, IL-6, TNF-α, and IL-10. Results: RME inhibited β-hexosaminidase release and synthesis of IL-4 and TNF-α in RBL-2H3 by the anti-DNP IgE plus DNP-HSA stimulation. In addition, RME inhibited the production of NO and the gene expression of IL-1β, iNOS, IL-6, and TNF-α in LPS-stimulated RAW 264.7 cells. Conclusion: From these results, we concluded that RME possesses anti-allergic activity and anti-inflammatory activity due to the inhibition of mast cells and macrophage function.

Inhibitory Effects of Allergic Reaction of Aqueous Extract of Salviae Radix Root by Anal Therapy (항장요법(肛腸療法)에 의한 단삼추출액(丹蔘抽出液)의 알레르기반응 억제효과(抑制效果))

  • Cho, Jung-Youn;Moon, Seok-Jae;Moon, Goo;Won, Jin-Hee;Kim, Hyung-Min
    • The Journal of Korean Medicine
    • /
    • v.20 no.1 s.37
    • /
    • pp.11-21
    • /
    • 1999
  • Dansam, the root of Salvia miltiorrhiza BGE. (Labiatae), has a bitter and a slightly 'cold' property. and is nontoxic. It has been used for the treatment of diseases such as tumors, bruises, gynecologic diseases, menoxenia, anemia and so forth. As an oriental medicine pathway, anal therapy has many kinds of treatments, Retention enema is one the most useful, advantageous methods of anal therapy. This experiment was performed in order to study the effect of an aqueous extract of Salviae radix root(SRRAE) on Immediate type allergic reaction by Retention enema. The results were obtained as follows, 1. SRRAE inhibited compound 48/80-induced Immediate type allergic reaction 100% with the dose of 0.1g/kg by anal treatment. However, SRRAE showed no significant inhibitory effect on the same reaction by oral treatment. When mice were pretreated with SRRAE at a concentration ranging from 0.001 to 1.0g/kg, by intra-anal treatment, the serum histamine levels were reduced in a dose-dependent manner. 2. SRRAE also inhibited by 79.8% local cutaneous allergic reaction activated by anti-dinitrophenyl (DNP) IgE. 3. SRRAE close-dependently inhibited the histamine release from rat peritoneal mast cells(RPMC) by anti-DNP IgE., but SRRAE not inhibited compound 48/80-induced histamine release. 4. SRRAE dose-dependently inhibited tumor necrosis $factor-\;{\alpha}$ $(TNF-\;{\alpha}$) production from RPMC by anti-DNP IgE. Moreover, the level of cAMP in RPMC, when SRRAE was added, significantly increased compared with that of a normal control. According to the above results, anal therapy(Retention enema) of SRRAE may be beneficial in the treatment of systemic and local Immediate type allergic reactions.

  • PDF

Effect of Saururus chinensis (Lour.) Baill. Aquacupuncture on Anaphlylaxis in Mice (삼백초약침액(三白草藥鍼液)이 아나필락시스에 미치는 영향)

  • Choi, Kyu-Jung;Lee, So-Young;Kang, Kyung-Hwa;Lee, Yong-Tae;Song, Choon-Ho
    • Korean Journal of Acupuncture
    • /
    • v.21 no.3
    • /
    • pp.97-104
    • /
    • 2004
  • Objective We investigated the effect of Saururus chinensis (Lour.) Baill. aquacupuncture (SCB) on anaphylaxis in mice. Methods : We conformed compound 48/80-induced mesenteric mast cell degranulation, active systemic anaphylatic shock and histamine release. Also observed acetic acid-induced vascular permeability and anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. Results : SCB inhibited mesenteric mast cell degranulation and active systemic anaphylatic shock induced by compound 48/80 dose-dependently. When SCB was pretreated by intra-peritoneal injection, the serum histamine levels were reduced. SCB also significantly inhibited acetic acid-induced vascular permeability. In addition, SCB showed a significant inhibitory effect on anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. Conclusion : These results indicated that SCB inhibits anaphylatic reaction.

  • PDF

Anti-allergy Action of Soeumin Kwakhyangjeonggisan (소음인(少陰人) 곽향정기산(藿香正氣散)의 항(抗) Allergy 작용(作用))

  • Ahn, Bo-kook;Song, Jeong-mo
    • Journal of Sasang Constitutional Medicine
    • /
    • v.13 no.3
    • /
    • pp.75-88
    • /
    • 2001
  • The purpose of this research was to investigate the effects of Kwakhyangjeonggisan (KJS) on the anti-allergic action. In the present study, we examined the effect of KJS on type I and type IV allergic reaction. KJS inhibited the systemic anaphylaxis induced by compound 48/80 and platelet activating factor (PAF), and inhibited the passive cutaneous anaphylaxis (PCA) induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin (HSA) in vivo. In addition, KJS dose-dependently inhibited the release of histamine from peritoneal mast cells in rat. Also, KJS inhibited the delayed type hypersensitivity (DTH) induced by SRBC and the contact dermatitis induced by dinitrofluorobenzene (DNFB). KJS inhibited the proliferation of splenocytes, the subpopulation of B220+ cells and CD4+CD8-(Th) cells in splenocytes and the production of γ-interferon in serum and splenocytes. These findings suggest that KJS prevented the type I allergy by the inhibition of histamine release from mast cells and the type IV allergy by the inhibition of γ-interferon production and B lymphocytes subpopulation. These results indicate that KJS may be useful for the prevention and treatment of type I and type IV allergy related disease.

  • PDF