The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Inhibitory Effect of Rhododendron Mucronulatum Root Extract on Allergic Inflammation

진달래 뿌리 추출물의 알레르기 염증 억제 효과

  • 장시성 (가천대학교 한의과대학) ;
  • 이대중 (가천대학교 한의과대학) ;
  • 송지훈 (제주 한의약 연구원) ;
  • 박도휘 (가천대학교 한의과대학) ;
  • 전찬용 (가천대학교 한의과대학) ;
  • 황귀서 (가천대학교 한의과대학)
  • Received : 2022.03.04
  • Accepted : 2022.04.01
  • Published : 2022.03.30
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Abstract

Objective: In this study, we investigated the protective effect of rhododendron mucronulatum extract (RME) on allergic reactions and inflammation. Methods: The effect of RME was determined using ELISA and RT-PCR in RBL-2H3 mast cells and RAW 264.7 macrophage cells. We determined cell viability, β-hexosaminidase release, and the synthesis of IL-4 and TNF-α in RBL-2H3 cells. In addition, we determined NO from RAW 264.7 and the gene expression of IL-1β, iNOS, IL-6, TNF-α, and IL-10. Results: RME inhibited β-hexosaminidase release and synthesis of IL-4 and TNF-α in RBL-2H3 by the anti-DNP IgE plus DNP-HSA stimulation. In addition, RME inhibited the production of NO and the gene expression of IL-1β, iNOS, IL-6, and TNF-α in LPS-stimulated RAW 264.7 cells. Conclusion: From these results, we concluded that RME possesses anti-allergic activity and anti-inflammatory activity due to the inhibition of mast cells and macrophage function.

Keywords

References

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