• Title/Summary/Keyword: D22S280

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No Associations between Schizophrenia and D22S280 Marker on Synapsin III Gene in Korean Males (한국인 남자에서 Synapsin III 유전자의 D22S280 표지자와 정신분열병의 연합연구)

  • Lee, Yu-Sang;Park, Chong-Won;Lee, Seung-Yeoun;Lee, Suk-Jin;Park, Yong-Bum;Shin, Yoon-Sik;Yoo, Jang-Keun;Hong, Kyung Sue;Yang, Byung-Hwan
    • Korean Journal of Biological Psychiatry
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    • v.13 no.4
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    • pp.260-266
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    • 2006
  • Objectives : Synapsin III near VCFS region on chromosome 22q affects. It could be an interesting candidate gene for schizophrenia. D22S280 is a highly polymorphic genetic marker residing in synapsin III. We examined association of D22S280 marker on synapsin III with Korean patients with schizophrenia. Methods : The subjects were 46 male Korean patients with schizophrenia and 60 male normal controls. Using polymerase chain reaction, gel electrophoresis, ABI 310 genetic analyzer, and GeneScan Collection 3.1 software, we confirmed genotypes of D22S280 marker. We examined Hardy-Weinberg equilibrium and case-control association using SAS/Genetic 9.1.3. Results : Genotypes of both schizophrenia and control groups were in Hardy-Weinberg equilibrium. We could not find any significant statistical differences in allele-wise(${\chi}^2$=10.4, df=6, p=0.098) and genotype-wise (${\chi}^2$=22.1 df=19, p=0.258) analyses of D22S280 marker between schizophrenia and normal controls. Individual allele analyses with df=1 showed significant differences in A1(p=0.025) and A7(p=0.034) allele, which were not significant following Bonferroni corrections(A1:p=0.177, A7:p=0.235). Conclusion : We couldn't find any association between schizophrenia and the synapsin III gene. Given the small number of subjects studied, further investigations are needed.

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Effects of Photoperiod and Light Intensity on the Growth and Glucosinolates Content of Three Brassicaceae Species in a Plant Factory (식물공장에서 광주기 및 광강도가 십자화과 3종의 생육과 글루코시놀레이트 함량에 미치는 영향)

  • Kim, Sunwoo;Bok, Gwonjeong;Shin, Juhyung;Park, Jongseok
    • Journal of Bio-Environment Control
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    • v.31 no.4
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    • pp.416-422
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    • 2022
  • This study was conducted to investigate the effect of each light intensity and photoperiod combination on the growth and glucosinolates (GSLs) content of three species of Brassicaceae plants under the same daily light integral (DLI) conditions. Seeds of leaf mustard (Brassica juncea (L.) Czern.), red mustard(Brassica juncea L.) and kale (Brassica oleracea L. var. acephala (DC.) Alef.) were sown in a rockwool cubes and grown for three weeks. DLI was set to 10 mol·m-2·d-1 and treated with 10h-280, 14h-200, 18h-155, 22h-127 µmol·m-2·s-1 for three weeks. As a result at 14h-200 µmol·m-2·s-1 treatment, shoot fresh/dry weight, the number of leaves, and leaf area were increased in leaf mustard and kale but there was no significant difference in other treatments. In the total GSLs content, the treatment of 14h-200 µmol·m-2·s-1 increased significantly 139.95, 135.87, 154.03% compared to 10h-280, 18h-155, 22h-127 µmol·m-2·s-1 treatment in red mustard, and 14h-200 µmol·m-2·s-1 treatment increased significantly 132.96, 132.96, 134.03% compared to other treatments in kale. In red mustard, the treatment of 18h-155 µmol·m-2·s-1 showed an increase in shoot fresh/dry weight and the total GSLs contents than other photoperiods and 14h-200 µmol·m-2·s-1 treatment, the number of leaves significantly 15.62, 12.12, and 32.14% higher than other photoperiods. Since the DLI response is different depending on species even for similar Brassicaceae crops, it is necessary to get more detailed results by conducting optical light quality studies and deriving optimal DLI conditions to achieve minimum power consumption and maximum efficiency.

Isolation, Identification, and Characterization of Bacillus spp. from the Traditionally fermented Cheonggukjangs in the Gyeonggi and the Gangwon Provinces (경기.강원 지역의 전통발효 청국장으로부터 Bacillus균주의 분리 동정 및 특성 분석)

  • Lee, Nam-Keun;Jeon, Eun-Hee;Lee, Hyo-Jin;Cho, Il-Jae;Hahm, Young-Tae
    • Applied Biological Chemistry
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    • v.49 no.4
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    • pp.276-280
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    • 2006
  • Traditionally fermented Cheonggukjangs were collected from Gyeonggi and Gangwon provinces and 22 strains were isolated and identified by using 165 rDNA sequences. Most of the identified strains were Bacillus subtilis and B. licheniformis, B. subtilis and B. licheniformis are dominant in the Gyeonggi area and B. licheniformis in the Gangwon area. In the growth pattern of the isolated strains, the duration of lag phase was generally 5 to 7 hours and stationary phase was reached after 23 to 40 hours of incubation. Total cell populations at the stationary phase were between $1{\times}10^6\;CFU/ml$ and $5{\times}10^7\;CFU/ml$. The fermenting ability of carbohydrates of isolates showed some differences among the regions. The isolated strains from Yong-In, Gyeonggi showed higher fermenting abilities with D-xylose, xylitol, D-tagatose and Methyl-$\alpha$-D-mannopyranoside. D-lactose, D-tagatose, D-xylose, Methyl-$\alpha$-D-mannopyranoside, amygdalin, arbutin, esculin and 2-keto-gluconate were well fermented with the An-Seong's strains; L-rhamnose, inositol, D-mannitol, D-sorbitol, celibiose and gluconate with the Kawang-Ju's stains; and D-lactose with the Odaesan's strains.

Vibration Power Improvement Method of Curved Beam Based Actuator Using Finite Element Analysis (유한요소해석을 이용한 곡면보 기반 진동체의 진동력 향상 방법)

  • Park, J.S.;Na, S.D.;Sung, K.W.;Kim, Myoung Nam
    • Journal of Korea Multimedia Society
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    • v.22 no.2
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    • pp.271-280
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    • 2019
  • Recently, hearing loss patients have been increasing to excessive use of various multimedia devices. One of the hearing rehabilitation systems, bone conduction hearing aid can be used to conductive deafness patients efficiently. However, the conventional bone conduction hearing systems has some problems such as skin diseases, repulsion of patients, and vibration power reduction by skin damping. In this paper, to overcome the conventional problems, we proposed power improvement method by curved beam diaphragm. The proposed method is skin attachment system which is non-implantable, and then the power of transducer is improved by the proposed method. In order to improve the vibration power of diaphragm, variable that has correlation with displacement are extracted, the diaphragm designed by extracted variable. To verify efficient of the proposed method, experiment conducted by finite element analysis. As a result of, the proposed method confirmed improved power to compare with the conventional method and proposed method.

Effect of Cysteamine on In Vitro Maturation, Fertilization and Culture of Porcine Oocytes

  • Yi, Y. J;Kim, M. Y.;Lee, S. H.;T. S. Min;D. I. Jin;Park, C. S.
    • Korean Journal of Animal Reproduction
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    • v.27 no.4
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    • pp.275-280
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    • 2003
  • This study was carried out to investigate the effect of cysteamine addition during in vitro maturation, fertilization and culture of porcine oocytes. Oocytes were matured for the first 22 h in mTCM -199 media supplemented with or without 150 $\mu$M cysteamine. They then were matured for an additional 22 h in mTCM-199 media without hormones supplemented with or without 150 $\mu$M cysteamine. When cumulus-oocyte complexes (COCs) were matured in the mTCM-199 media supplemented with cysteamine, the rates of GVBD and maturation (metaphase II) were enhanced as compared to the media without the addition of cysteamine. Also, when COCs were matured in the mTCM-199 media supplemented with cysteamine, the rates of sperm penetration, male pronucleus formation, cleavage and blastocyst formation after in vitro fertilization were enhanced as compared to the media without the addition of cysteamine. In conclusion, it was suggested that oocytes matured for the first 22 h in mTCM-199 media supplemented with 150 $\mu$M cysteamine increased the rates of metaphase II, sperm penetration, male pronucleus and blastocyst formation were higher as compared to the media without addition of cysteamine.

The Status of Blood and Body Fluid Exposure and Affecting Factors among Nursing Students including Knowledge, Performance regarding Standard Precautions (간호대학생의 임상실습중 혈액 및 체액 노출현황과 영향 요인 - 감염관리 지식 및 수행도 중심으로-)

  • Kim, Hee-Jung;Kim, Nam-Cho
    • The Journal of Korean Society for School & Community Health Education
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    • v.15 no.3
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    • pp.17-30
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    • 2014
  • Objectives: The purpose of this study was to investigate the status of exposure to blood and body fluid and factors affecting on blood and body fluid exposure of nursing students including knowledge, performance regarding standard precautions. Methods: 280 nursing students in D-city and S-city participated in this study. Data collection was conducted from January to March 2012. Students' knowledge and performance on standard precautions of infection control in hospitals were measured using the measurement developed by Hong(2010). Data analysis was performed using descriptive statistics, t-test, one way-ANOVA, ${\chi}^2-test$, Pearson correlation coeficient, logistic regression by the SPSS WIN 18.0 program. Results: 58.6 percent of subjects experienced a exposure to blood and body fluid. The level of nursing students' knowledge level regarding standard precautions was $22.44{\pm}2.11$ and performance level was $4.31{\pm}0.50$. The factors affecting on blood and body fluid exposure of nursing students were knowledge level and education type about infection control. Conclusions: Based on the findings of this study, to decrease the exposure to blood and body fluid of nursing students, a proper education need to be stressed to improve nursing students' infection control knowledge and awareness.

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Sentiment Analysis and Issue Mining on All-Solid-State Battery Using Social Media Data (소셜미디어 분석을 통한 전고체 배터리 감성분석과 이슈 탐색)

  • Lee, Ji Yeon;Lee, Byeong-Hee
    • The Journal of the Korea Contents Association
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    • v.22 no.10
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    • pp.11-21
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    • 2022
  • All-solid-state batteries are one of the promising candidates for next-generation batteries and are drawing attention as a key component that will lead the future electric vehicle industry. This study analyzes 10,280 comments on Reddit, which is a global social media, in order to identify policy issues and public interest related to all-solid-state batteries from 2016 to 2021. Text mining such as frequency analysis, association rule analysis, and topic modeling, and sentiment analysis are applied to the collected global data to grasp global trends, compare them with the South Korean government's all-solid-state battery development strategy, and suggest policy directions for its national research and development. As a result, the overall sentiment toward all-solid-state battery issues was positive with 50.5% positive and 39.5% negative comments. In addition, as a result of analyzing detailed emotions, it was found that the public had trust and expectation for all-solid-state batteries. However, feelings of concern about unresolved problems coexisted. This study has an academic and practical contribution in that it presented a text mining analysis method for deriving key issues related to all-solid-state batteries, and a more comprehensive trend analysis by employing both a top-down approach based on government policy analysis and a bottom-up approach that analyzes public perception.

Evidence of Significant Effects of Stunning and Chilling Methods on PSE Incidences

  • Park, B.Y.;Kim, J.H.;Cho, S.H.;Hah, K.H.;Lee, S.H.;Choi, C.H.;Kim, D.H.;Lee, J.M.;Kim, Y.K.;Ahn, J.N.;Hwang, I.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.2
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    • pp.257-262
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    • 2007
  • The current study was conducted to investigate the optimum stunning voltage and chilling regime with emphasis on reduction in pale, soft and exudative (PSE) pork. The experiments were conducted at seven Korean major pig abattoirs using a total of 91,082 industrial population. Frequencies of PSE meat was found to be significantly (p<0.05) increased as stunning voltage was elevated from 220-240 (13.14%), 250-280 (29.32%) to 430 volts (36.74%). Chilling methods after slaughter, either with cold water showing or rapid chilling reduced PSE meat by 22% compared to a classic chiller-based slow chilling regime. The current study also revealed that chiller temperature during the first 90 minutes had a significant (p<0.001) effect on PSE incidences. Pigs chilled between -5 to $7^{\circ}C$ resulted in the lowest PSE meat (17.8%), followed by higher than $7^{\circ}C$ (21.3%) and lower than $-5^{\circ}C$ (37.5%). The current data implies that low voltage stunning method (eg., 220-240 volts), followed by rapid chilling regime, maintaining chiller temperature between approximately -5 to $7^{\circ}C$ could reduce PSE incidences.

A Study on Blood Glucose Level and Self Management among Community Dwelling Type II Diabetes Patients (당조절이 되지 않는 지역사회 제2형 당뇨환자에서의 혈당수준과 자가관리 실천정도 간의 관계)

  • Park, Ju-Young;Lee, Tae-Yong;Jang, Keong-Sug;Oh, Hee-Young
    • Korean Journal of Adult Nursing
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    • v.22 no.3
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    • pp.271-280
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    • 2010
  • Purpose: The purpose of this study was to examine relationships among blood glucose, HbA1c, and self management comparing these with general and illness characteristics of subjects with Type II Diabetes living in the community. Methods: Using a comprehensive survey developed for the study, data were collected from 82 type II diabetes patients who were registered at five community health departments. The variables of self management, blood glucose and HbA1c were assessed by nurses. Data were analyzed with descriptive statistics including t-test, ANOVA and Pearson's correlation coefficient to compare self management and level of HbA1c by subject's general and illness characteristics and to examine the relationships among variables. Results: About 73.2% of the subjects' HbA1c were 7.0% or higher and 54.9% of subjects' blood glucose were 200 mg/dL or higher. The level of self management was moderate. Most frequently perceived reasons for failure of blood glucose control were dietary failure (32.9%). There was significant relationship between self management and HbA1c(r=-.223, p=.040). The mean score of self management were higher among female (t=-2.37, p=.021), who are not on diabetes medication (t=6.70, p=.011). Conclusion: Comprehensive intervention is needed to improve dietary self management, especially for male and those who is on diabetes medication.

Extrusion-cooking Using Twin-screw Extruder on Cordyceps Pruinosa (이축 압출 성형기를 이용한 붉은자루 동충하초의 압출 성형)

  • Kim D. E.;Sung J. M.;Kang W. S.
    • Journal of Biosystems Engineering
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    • v.30 no.1 s.108
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    • pp.8-16
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    • 2005
  • The extrusion-cooking condition on Cordyceps pruinosa was designed using twin-screw extruder. Response surface methodology (RSM) was used to investigate extrusion-cooking using a central composition design with varying die temperature $(114-146^{\circ}C)$, feed moisture $(22-38\%)$, feed rate (4-14 ka/h) and screw speed (120-280 rpm). System parameters (die pressure and specific mechanical energy (SME)) and extrudate parameters (density and water solubility index (WSI)) were statically analyzed using RSH. Die pressure was significantly affected by temperature, moisture contents and feed rate. SM was affected by screw speed and feed rate. When die temperature is $130^{\circ}C$ and moisture content $25\%$, the optimum pressure is shown. SME is about 20 Wh/kg, when feed rate is $10\~12kg/min$ and screw speed $200\~250rpm$. WSI was affected by temperature and moisture contents. Density was not affected by any factor. WSI increases by $7\%$ from about $23\%$ to about $30\%$, as temperature is raised from $120^{\circ}C\;to\;140^{\circ}C$. The WSI of Cordyceps pruinosa pulverized after extruding (PE) is about $26.97\%$ higher than that of raw material and $10\%$ higher than that of pulverized after drying (PD). The content of unsaturated fatty acid were not significantly different in PD and PE. Anti-oxidative activity of PE was 1.67-2.2 times higher than that of PD in Cordyceps pruinosa using 1- dipheny1-2-picrylhydrazyl method (DPPH).