• 제목/요약/키워드: D-loop variation

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Single Stranded Conformation Polymorphism 분석에 의한 돼지 Duroc 품종의 미토콘드리아 DNA 유전적 변이 (Genetic Variation of Mitochondrial DNA in Duroc (Sus Scrofa) Using Single Stranded Conformation Polymorphism Analysis)

  • 조인철;정용환;정진관;성필남;김병우;이정규;전진태
    • Journal of Animal Science and Technology
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    • 제45권6호
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    • pp.911-916
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    • 2003
  • 돼지 Duroc 품종의 mitochondria DNA D-loop전체 유전자를 증폭하기 위하여 많은 동물에서 고도로 상동성이 높은 tRNA-Pro와 tRNA-Phe 염기서열 일부를 이용하여 oligonucleotide primer를 제작하였다. 그 결과 Duroc 품종의 D-loop 전체 유전자는 1,145 base pairs 였으며, 그 중간위치에 10bp의 Sus Scrofa-specific sequence (TACACGTGCG)가 10개 존재하고 있었다. 돌연변이 검출을 위하여 가장 변이가 심한 지역을 primer 제작하여 345 bp의 DNA 단편을 증폭하였으며, Single Stranded Conformation Polymorphism(SSCP) 분석은 8% polyacrylamide gel에서 200 V, 16시간 전기영동하여 ethidium bromide (EtBr)로 10분간 염색하여 UV image analyzer로 관찰하였다. 그 결과 두 개의 서로 다른 밴드유형을 관찰하였으며, 21개 부위에서 염기서열 변이가 관찰되었다. 이러한 결과는 유전적 다양성 변이를 검출하는데 SSCP 분석이 유용한 도구라고 사료된다.

Phylogenetic Analysis of a Privately-owned Korean Native Chicken Population Using mtDNA D-loop Variations

  • Hoque, M.R.;Choi, N.R.;Sultana, H.;Kang, B.S.;Heo, K.N.;Hong, S.K.;Jo, C.;Lee, Jun-Heon
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권2호
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    • pp.157-162
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    • 2013
  • The use of Korean native chicken is increasing, and the discovery of new genetic resources is very important from both economic and genetic conservation points of view. In this study, mtDNA D-loop sequences from 272 privately-owned Korean native chickens from a Hyunin farm were investigated. Seventeen nucleotide substitutions were identified from the sequence analysis and they were classified as 6 haplotypes. Previously investigated haplotypes in five Korean native chicken populations have been compared with the Hyunin chicken population. The results indicated that two haplotypes, H10 and H15, in the Hyunin chicken population were not previously identified in other Korean native chicken populations, representing 33.09% (90/272) and 1.1% (3/272) of the Hyunin population, respectively. On the other hand, four other haplotypes were identical to those of a previous study of Korean native chicken populations. This result is indicative of conservation strategies of Hyunin chicken populations for expanding the genetic diversity in the Korean native chicken population.

Mitochondrial D-Loop Variations for Discrimination of Commercial Korean Native Chicken Populations

  • Sultana, Hasina;Hoque, Md. Rashedul;Seo, Dong-Won;Kang, Bo-Seok;Heo, Kang-Nyeong;Jo, Cheorun;Lee, Jun-Heon
    • 한국가금학회지
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    • 제39권4호
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    • pp.311-315
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    • 2012
  • The increasing demand for Korean native chicken meat indicates that the discovery of haplotypes is very important from both economic and conservation points of view. In this study, mtDNA D-loop sequences from two crossbred Korean native chicken populations of 138 individuals were investigated. Twenty six nucleotide substitutions were identified from sequence analysis and were classified into 12 haplotypes. The haplotype H_8 represents 73.47% of Woorimatdag (chicken population) sequences, which were identified in all five Woorimatdag chicken populations investigated. The H_7 haplotype (Dhap1) for D population covers 45% sequences, which indicate maternal inheritance from black Korean native chicken. On the other hand, Chap3 and Chap4 for C population are specific haplotypes, as H_5 and H_2, respectively. Based on the network profiles, six SNPs (C199T, A239G, G242A, A291G, T330C and C391A) of the D-loop region are effective markers for discrimination between Woorimatdag and Hanhyup chicken populations. Also, the phylogenetic analyses of Woorimatdag and Hanhyup chicken populations were used to identify the genetic relationships among the haplotypes. The results presented here can be used for developing molecular markers to discriminate between two commercial Korean native chickens.

Time Dependent Correlation Function과 그의 응용에 관한 연구 (Some Study on Time Dependent Correlation Function and Its Applications)

  • 안수길
    • 대한전자공학회논문지
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    • 제10권6호
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    • pp.25-44
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    • 1973
  • 원인과 결과간의 위상과 work사이의 관계를 correlation function의 견지에서 검토하였고 continuous signal에 포함되어 있는 redundancy를 지적하여 sampling취급의 근거를 auto correlation면에서 보였다. 두 신호사이의 Correlation의 시간변동을 보여줄 수 있는 Time Dependent Correlation Function을 정의하여 PLL회로에서 그 유편성을 보였다. 끝으로 다상포낙선검파법에 의한 특성의 개선을 T.D.Correlation Furction에 의한 Correlation Analysis를 통하려 입증하였다.

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Evaluating genetic diversity and identifying priority conservation for seven Tibetan pig populations in China based on the mtDNA D-loop

  • Ge, Qianyun;Gao, Caixia;Cai, Yuan;Jiao, Ting;Quan, Jinqiang;Guo, Yongbo;Zheng, Wangshan;Zhao, Shengguo
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권12호
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    • pp.1905-1911
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    • 2020
  • Objective: Tibetan pigs, an excellent species unique to China, face serious threats, which in turn affects the development and utilization of the outstanding advantages of plateau hypoxia adaptability and reduces their genetic diversity. Therefore, a discussion of measures to conserve this genetic resource is necessary. The method, based on genetic diversity, genetic divergence and total genetic contribution rate of population, reflects the priority conservation order and varies depending on the three different purposes of conservation. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces and downloaded 564 mtDNA D-loop sequences from three indigenous pig breeds in Qinghai, Sichuan, and Yunnan Provinces distributed near the Tibetan pigs. Results: We analyzed three different aspects: Changdu Tibetan pigs have the highest genetic diversity, and from the perspective of genetic diversity, the priority conservation is Changdu Tibetan pigs. Hezuo Tibetan pigs have the highest genetic contribution, so the priority conservation is Hezuo Tibetan pigs in the genetic contribution aspect. Rkaze Tibetan pigs were severely affected by indigenous pig breeds, so if considering from the perspective of introgression, the priority conservation is Rkaze Tibetan pigs. Conclusion: This study evaluated genetic diversity and comprehensively assessed conservation priority from three different aspects in nine Tibetan pig populations.

순환유동층 재순환부 내 고체흐름 특성에 대한 시스템 압력의 영향 (Effect of Pressure on Solids Flow Characteristics in Recycle System of a Circulating Fluidized Bed)

  • 김성원;김상돈
    • Korean Chemical Engineering Research
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    • 제48권2호
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    • pp.198-204
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    • 2010
  • 가압순환유동층 적용을 위해 루프씰(loop-seal: 내경 0.10 m)을 갖는 고체재순환부(직경 0.10 m, 높이 2.25 m)에서 층 물질로 silica sand 입자($d_p=240{\mu}m$, ${\rho}_s=2582kg/m^3$)를 사용하여 시스템 압력 변화(0.10~0.71 MPa)에 따른 고체흐름 특성을 연구하였다. 루프씰을 통한 고체질량플럭스는 공기주입량이 증가할수록 선형적으로 증가하였고, 동일한 공기주입속도에 대해 시스템 압력이 증가할수록 증가하였다. downcomer 내 압력변이는 시스템 압력이 증가할수록 동일한 공기주입속도에 대해 증가하였고, 흐름 내 고체속도 및 기체 속도 또한 증가하였다. 고체질량플럭스로부터 downcomer 에서의 압력변이를 예측할 수 있는 상관관계식을 Transportation number와 Pressure drop number를 이용하여 제안하였다. 루프씰에서의 압력강하는 시스템 압력에 관계없이 고체질량플럭스가 증가할수록 증가하였다. 각각의 시스템 압력에서 공기주입속도 변화에 따른 고체질량플럭스 및 Transportation number를 예측할 수 있는 상관관계식을 제안하였다.

A Clock and Data Recovery Circuit with Adaptive Loop Bandwidth Calibration and Idle Power Saved Frequency Acquisition

  • Lee, Won-Young;Jung, Chae Young;Cho, Ara
    • JSTS:Journal of Semiconductor Technology and Science
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    • 제17권4호
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    • pp.568-576
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    • 2017
  • This paper presents a clock and data recovery circuit with an adaptive loop bandwidth calibration scheme and the idle power saved frequency acquisition. The loop bandwidth calibration adaptively controls injection currents of the main loop with a trimmable bandgap reference circuit and trains the VCO to operate in the linear frequency control range. For stand-by power reduction of the phase detector, a clock gating circuit blocks 8-phase clock signals from the VCO and cuts off the current paths of current mode D-flip flops and latches during the frequency acquisition. 77.96% reduction has been accomplished in idle power consumption of the phase detector. In the jitter experiment, the proposed scheme reduces the jitter tolerance variation from 0.45-UI to 0.2-UI at 1-MHz as compared with the conventional circuit.

Numerical simulation of a toroidal single-phase natural circulation loop with a k-kL-ω transitional turbulence model

  • Yiwa Geng;Xiongbin Liu;Xiaotian Li;Yajun Zhang
    • Nuclear Engineering and Technology
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    • 제56권1호
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    • pp.233-240
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    • 2024
  • The wall friction correlations of oscillatory natural circulation loops are highly loop-specific, making it difficult to perform 1-D system simulations before obtaining specific experimental data. To better predict the friction characteristics, the nonlinear dynamics of a toroidal single-phase natural circulation loop were numerically investigated, and the transition effect was considered. The k-kL-ω transitional turbulence and k-ω SST turbulence models were used to compute the flow characteristics of the loop under different heating powers varying from 0.48 to 1.0 W/cm2, and the results of both models were compared with previous experiments. The mass flow rates and friction factors predicted by the k-kL-ω model showed a better agreement with the experimental data than the results of the k-ω SST model. The oscillation frequencies calculated using both models agreed well with the experimental data. The k-kL-ω transitional turbulence model provided better friction-factor predictions in oscillatory natural circulation loops because it can reproduce the temporal and spatial variation of the wall shear stress more accurately by capturing the movement of laminar, transition turbulent zones inside unstable natural circulation loops. This study shows that transition effects are a possible explanation for the highly loop-specific friction correlations observed in various oscillatory natural circulation loops.

나카가미-m 페이딩 채널 하에서 PLL 이득에 따른 DS/CDMA의 성능 분석 (Performance Analysis of DS/CDMA with PLL Gain under the Nakagami-m Fading Channel)

  • 강찬석;박진수
    • 대한전자공학회논문지TE
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    • 제37권3호
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    • pp.53-59
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    • 2000
  • 이동통신환경에서 수신신호의 진폭과 위상성분은 다중경로에 의한 페이딩(Fading) 영향을 받는다. 본 논문에서는 송수신 신호의 위상 차를 위상에러로 가정하고 Tikhonov 확률밀도함수를 이용하여 PLL(Phase Locked Loop)이득의 변화에 대한 DS/CDMA(Direct Sequence/code Division Multiple Access) DPSK(Differential Phase Shift Keying)시스템의 성능을 분석하였다. 그 결과, 위상에러를 고려하지 않은 DPSK시스템과 비교하여 수신기의 PLL이득을 조정함으로써 시스템의 성능을 향상시킬 수 있음을 알 수 있었다. 시스템의 비트 포율이 10-2에서 PLL이득이 1㏈에서 4.8㏈, 7㏈에서는 0.4㏈의 성능차를 보이며 30㏈에서는 두 시스템의 성능이 일치하게되어 PLL이 요구하는 이득의 상한이 30㏈임을 입증하였다.

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