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http://dx.doi.org/10.5187/JAST.2003.45.6.911

Genetic Variation of Mitochondrial DNA in Duroc (Sus Scrofa) Using Single Stranded Conformation Polymorphism Analysis  

Cho, I.C. (National Jeju Agricultural Experiment Station, R.D.A.)
Jung, Y.H. (National Jeju Agricultural Experiment Station, R.D.A.)
Jung, J.K. (National Jeju Agricultural Experiment Station, R.D.A.)
Seong, P.N. (National Jeju Agricultural Experiment Station, R.D.A.)
Kim, B.W. (Division of Applied Life Science, Gyeongsang National University)
Lee, J.G. (Division of Applied Life Science, Gyeongsang National University)
Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Journal of Animal Science and Technology / v.45, no.6, 2003 , pp. 911-916 More about this Journal
Abstract
The mitochondrial DNA(mtDNA) D-loop region was amplified from Duroc(Sus scrofa) by polymerase chain reaction(PCR). The oligonucleotide primer used to amplify the Sus scrofa mtDNA D-loop region was designed using tRNA-Pro and tRNA-Phe sequence in mtDNA regions highly conserved in many other animal species. There were 1,145 base pairs(bp) in the D-loop region. The middle of the region contained 10 tandem repeat of an 10-bp Sus scrofa-specific sequence, TACACGTGCG. We designed primers for PCR-mediated single stranded conformation polymorphism(SSCP) analysis that amplified a 345 bp fragment, which contained the most variable region according to our sequencing data. SSCP analysis of denatured amplification products was carried out by polyacrylamide(8%) gel electrophoresis followed by ethidium bromide staining. The SSCP analysis identified two band patterns(A and B) and comparision of these two nucleotide sequences identified 21 base substitutions. These results show that SSCP analysis of the D-loop region is useful for detecting the genetic polymorphism.
Keywords
D-loop; SSCP; Sus scrofa; Tandem repeat;
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