• Journal of Plant Biotechnology
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• v.46 no.1
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• pp.32-36
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• 2019

Calla lilies (Zantedeschia spp.) are monocotyledonous ornamental plants which belongs to the Araceae family. After the release of elite calla cultivar, an efficient propagation system is needed for commercial use. Despite the use of conventional propagation methods such as splitting of tubers and rhizomes of calla, rapid and efficient propagation system should be developed. In order to achieve this goal, stem segments contained apical meristems derived from calla lily cultivar (cv. Gag-si) were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinin and auxin. This was aimed at inducing embryogenic calluses, shoots and multiple shoots. As a result, about 25% of induction rates of yellow embryogenic calluses were observed with MS medium containing both $0.5mg{\cdot}L^{-1}\;NAA$ and $1.5mg{\cdot}L^{-1}\;BA$ as growth regulators. In the experiments involving the regeneration from embryogenic calluses through shoot formation, MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ showed the highest rates at approximately 85 ~ 90% with regard to the formation of shoots in calla. Moreover, multiple shoots needed for rapid propagation were generated when explants were cultured on MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ with 40% of formation rate. In this study, the combination of auxin and cytokinin showed positive effects on both the induction of embryogenic calluses, the formation of shoots as well as multiple shoots in calla. The regeneration system described here can contribute to the development of breeding programs of calla in the future.

• Analytical Science and Technology
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• v.34 no.6
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• pp.275-283
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• 2021

This study presents the development of cell-based microfluidic chips for the performance of acute eye irritation tests due to chemicals and examined some of their applications. Microfluidic chips were fabricated by photolithography and soft lithography, and they had three compartments with different areas for cell culture. Rabbit corneal epithelial cells were used for the eye irritation test. The death of cells cultured inside the chip was monitored at regular time intervals after treatment with an aqueous solution of chemicals, and the cell death rate constants were calculated based on the viability curve. The performance of the microfluidic chip was verified by examining the effects of cell-cell junctions, cell-substrate adhesion, and initial cell numbers compared to cell death rates. Eye irritation tests were performed at various concentrations of an aqueous solution of sodium dodecyl sulfate (SDS), a standard substance for the eye irritant test. The cells were exposed to the SDS aqueous solution for 300 s, and the resulting eye irritation was assessed by cell viability. Finally, the equation for calculating the toxicity score (TS) was derived based on the weighting factor for each compartment in the chip. The cell-based microfluidic chip developed in this study may be used for eye irritation tests from chemicals used in cosmetics and pharmaceuticals.

• 지역과문화
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• v.8 no.2
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• pp.49-68
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• 2021

The pandemic of COVID-19 also affected performing arts business and stimulated 'Untact' performances, which means online streaming of the shows including real-time streaming. This study sought to examine factors to the consumers' acceptance. The research framework was based on the VAM, a revised model of TAM for the ICT products and services. For the research, consumer survey was conducted, where ndependent variables are perceived usefulness, pleasure, technicalty and innovation resistance, and dependent variables are percieved value and acceptance. Smart PLS was used to test the hypothesis. The result shows that the significant factors were percieved usefulness(+), pleasure(+) and innovation resistance(-). The percieved technicalty was not significant, the major reason would be that the digital devices and the internet technology are percieved a commodity these days in Korea. Percieved value was significant factor to acceptance. This study is meaningful because it is about the new phenomenon of 'untact' performance through the VAM methodology and it examined the significant factors to the attitude from the perspective of benefits and costs. There is limit that this study didn't consider old peoples' attitude. It is the reason that the continuous researches are necessary.

• Journal of Platform Technology
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• v.12 no.1
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• pp.55-66
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• 2024

The K-POP market has become influential not only in culture but also in society as a whole, including diplomacy and environmental movements. As a result, various papers have been conducted based on machine learning to identify the success factors of idols by utilizing traditional data such as music and recordings. However, there is a limitation that previous studies have not reflected the influence of new media platforms such as Instagram releases, YouTube shorts, TikTok, Twitter, etc. on the popularity of idols. Therefore, it is difficult to clarify the causal relationship of recent idol success factors because the existing studies do not consider the daily changing media trends. To solve these problems, this paper proposes a data collection system and analysis methodology for idol-related data. By developing a container-based real-time data collection automation system that reflects the specificity of idol data, we secure the stability and scalability of idol data collection and compare and analyze the clusters of successful idols through a K-Means clustering-based outlier detection model. As a result, we were able to identify commonalities among successful idols such as gender, time of success after album release, and association with new media. Through this, it is expected that we can finally plan optimal comeback promotions for each idol, album type, and comeback period to improve the chances of idol success.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.567-573
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• 2001

Lactobacilli have been generally recognized as an important lactic acid bacteria present in the normal human intestinal flora. Fifty two Lactobacillus isolates were recovered from the feces of healthy Koreans whose age ranged from thirteen days after birth to 37 years. Among the isolates above, 17 isolates were tentatively identified as strains of Lactobacillus acidophilus and 3 isolates as strains of Lactobacillus casei. For their characterization, these twenty isolates were subjected to the experiments for the resistance to the artificial gastric juice, pH2.5 and bile salt. Interestingly, 3 strains survived pH 2.5 after 3 hour incubation in the artificial gastric juice with more than 75% of survival rate. L. acidophilus a-4 had the highest survival rate of 100%. Four strains including L. acidophilus a-3 grew gradually in MRS broth in the presence of the artificial bile salt. Cholesterol assimilation was also tested for the 20 isolates. The result showed that cholesterol concentration of the medium was reduced by 10 Lactobacillus isolates with more than 60%, as compared to the control. L. acidophilus a-2 had the highest reduction rate of 77%. Judging from these data obtained in vitro, some isolates ware likely to reach the lower small intestine after consumption without a significant loss of viability, suggesting that they had the potential to be developed as a probiotic culture which might lower the cholesterol level in human.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.11
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• pp.5206-5210
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• 2012

We studied the effect of food concentration on grazing, growth and fecundity of cyclopoid copepod Paracyclopina nana. Marine phytoplankton Tetraselmis suecica was used as a livefood for the copepod. Six stage compositions were used and food concentrations for the experiment were 0.5, 1, 2, 5, 10, 20, 40, 60 and $80{\times}10^4$ cells/mL. Range of food concentrations with 0.2, 0.5, 1, 2, 3 and $4{\times}10^4$ cells/mL were used for nauplii production experiment. Grazing rates of P. nana in all developmental stages on the different concentrations were increased with increasing diet concentration. While the growth of nauplius was not affected by increase of food concentration, food concentration outside of $1{\times}10^4$ to $5{\times}10^4$ cells/mL range negatively affected that of copepodite. Daily nauplii production was increased with increasing food concentration but $3{\times}10^4$ and $4{\times}10^4$ cells/mL treatments were not significantly different with $2{\times}10^4$ cells/mL treatment. As a result, optimum concentration of T. suecica for mass culture of P. nana was considered to be 5,000 cells/mL for nauplius stage, 10,000 cells/mL for copepodite stage and adult male, 20,000 cells/mL for adult female, respectively.

• Journal of the Economic Geographical Society of Korea
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• v.20 no.1
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• pp.34-48
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• 2017

The purpose of this study is to investigate the growth of major industries and effects of regional employment on the economic growth of Goyang city and Seongnam city in Gyeonggi Province. We examine the structural changes of the industrial specialization and factors of the growth in two regions by applying Location Quotients and dynamic shift-share analysis respectively. Both regions have been strengthened gradually in their industrial bases during the last 20 years since they have developed as the bed-towns for Seoul with the First Newtown Project of the Metropolitan Seoul area. These two regions have secured in the industrial specialization and competitiveness compare to other regions in the Metropolitan Seoul area. In particular, knowledge service industries have been agglomerated in both regions. Goyang city have secured in the medical welfare, culture and tourism of service sectors, while Seongnam city have secured in the information service and science technology service sectors. Therefore, each region might be differentiated regional foster industries in these sectors in order to succeed the economic development.

• Journal of Elementary Mathematics Education in Korea
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• v.20 no.1
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• pp.163-192
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• 2016

In this study, we analyzed what STEAM-related subject contents, except mathematical knowledge, are contained in 2009 revised elementary mathematics 6th grade textbooks and teacher's manuals. The STEAM-related subject contents in the textbooks and the teacher's manuals were examined by unit, by semester, and by strand of the content in the elementary school mathematics curriculum. The results are the following: First, in each unit and in each strand of mathematics, the most frequent STEAM-related subject content is storytelling, followed by the STEAM-related subject contents of technology and engineering, natural science, and social studies in order. On the other hand, the number of culture, physical education, music and fine arts contents is very small. Second, the number of STEAM-related subject contents in the textbook for the second semester(textbook 6-2) of the 6th grade year is 61 more than that in the textbook for the first semester(textbook 6-1). The number of non-storytelling STEAM-related subject contents in textbook 6-2 is 107, 2.7 times more than that in textbook 6-1. Third, the teacher's manual for textbook 6-1 is insufficient in complementing the textbook units which lack in STEAM-related subject contents, while the teacher's manual for textbook 6-2 is comparatively good in its complementing role. Therefore, it is recommended that we develop different STEAM materials for our 6th grade mathematics classes.

• Horticultural Science & Technology
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• v.16 no.3
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• pp.347-349
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• 1998

Slow sand filtration was modified and applied for the determination of eliminating efficacy of various fungi and for recommending an easy approach to growers. After 1,500 liter filtration, Fusarium oxysporum was eliminated by several substrates such as activated charcoal (92.5% elimination), silica (90.8%), vermiculite (90.5%), sand (82.3%), perlite (50.4%), and hydroball (21.2%). Silica was able to eliminate several fungi by maximal ratio, which was corresponded to Fusarium oxysporum 120 cfu/mL. Collectotrichum lagenarium 98 cfu/mL. Phytophthora capsici 82 cfu/mL, Botrytis cinerea 62 cfu/mL, Pythium spp. 42 cfu/mL, and Sclerotinia ssp. 52 cfu/mL. In this case, the change of EC was minor and pH was maintained to about 7. In deep flow culture of 'Ddooksum Cheokchookmyeon' lettuce and 'Seokwang' tomato, silica-, activated charcoal-, and vermiculite-based filtration system successfully eliminated Fusarium oxysporum and Phytophthora capsici from the nutrient solution. As a result, these plants were not diseased by ten weeks after inoculation. With this system, growers can easily control the root-zone fungi in the recirculating hydroponic system.

• Journal of Digital Convergence
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• v.12 no.12
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• pp.485-490
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• 2014

The purpose of this Study investigated corneal power, corneal astigmatism and corneal axis according to spherical equivalent of refractive error. We measured spherical equivalent, corneal power and corneal astigmatism in 100 subjects from January 2014 to July 2014. Measured spherical equivalent of refractive error were $-3.01{\pm}3.79D$, corneal power of $43.79{\pm}1.60D$ and corneal astigmatism of $-1.17{\pm}0.79D$ respectively. Prevalence of spherical equivalent of refractive error were as follows : myopes (61%), emmetropes (22%), hyperopes(17%). Corneal astigmatism of refractive error greater than +0.75D was 63% and prevalence of corneal astigmatism were as follows : with-the-rlue astigmatism (84.13%), against-the-rule astigmatism(9.52%) respectively. Corneal power by spherical equivalent increased from hyperopia to myopia. Between spherical equivalent of refractive error and the mean corneal power was significant correlation(r=-0.25, p=0.01). A correlation were found between corneal power and spherical equivalent of refractive error in adults. They have the highest distribution of prevalence myopia among the refractive error. When the refractive error was increased, we found that corneal power was steeper. It is recognized that this can be refractive error factor and correct visual function is considered.