• 산업경영시스템학회지
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• 제16권27호
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• pp.1-10
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• 1993

This study aims to find the creative management system for the Company-Wide Creativity Development(CWCD). And this study consider managemant culture to find CWCD system because managemant culture have influence on the specific character of the personality and beheavior of the system member. To consider managemant culture, this study attempt to compare Korea managemant culture with America, Japanese managemant culture. Through this study we know that we must throw the authoritarianism, the egoism and try to resurrect Korean independence to find CWCD system in korea companies.

• 한국IT서비스학회지
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• 제22권6호
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• pp.55-70
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• 2023

This study conducted a fuzzy set qualitative comparative analysis study on culture and arts information portals to ensure practical and universal use and activation of culture and arts information portals. The capabilities and influence of affordances and system literacy according to user experience regarding accessibility to cultural arts information portals are as follows. First, physical affordances in the culture and arts information portal were key conditions for the culture and arts information portal user experience regarding system accessibility, system understandability, system usability, system usability, and system literacy. Second, the functional affordance of the user experience in the cultural arts information portal was relatively low and unimportant, and when the functional affordance was low, the user's system accessibility, system understanding, system usefulness, system usability, and system literacy were increased. Third, if the user's functional affordance and sensory affordance are low in the culture and arts information portal, the system accessibility, system understanding, system usability, and system literacy of the culture and arts information portal users are increased. Fourth, cultural arts information portals must build an innovative system with a systematic approach appropriate for the user base by linking system literacy capabilities with a focus on physical affordances.

• 한국수정란이식학회지
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• 제25권4호
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• pp.259-262
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• 2010

Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per ${\mu}l$ in $20\;{\mu}l$ drop (20.5%) and 1.0 embryos per ${\mu}l$ in $10\;{\mu}l$ drop (20.6%). Based on these results, we compared drop culture system and 'MTC' system in terms of the developmental rate to the blastocyst stage. In $20\;{\mu}l$ medium volume, the 'MTC' system showed similar blastocyst formation rate when compared with drop culture system (20.2% versus 20.5%, respectively) while the 'MTC' system showed lower blastocyst formation rate than drop culture system in $10\;{\mu}l$ one (12.7% versus 20.0%, respectively). Therefore the $20\;{\mu}l$ MTC system may be an alternative incubation system for short-distance embryo transport without carrying the $CO_2$ incubator and this provides novel embryo culture device to clinical veterinary embryologists.

• Biomolecules & Therapeutics
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• 제10권4호
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• pp.281-286
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• 2002

In this study, we intended to get a preliminary data for establishing rat tracheal surface epithelial(RTSE) cell culture system as an experimental model for physiology and pharmacology of tracheal epithelial cells. Primary culture on the membrane support and application of the air-liquid interface system at the level of cell layer were performed. The cell growth rate and mucin production rate were measured according to the days in culture. The results were as follows: this culture system was found to manifest mucocilliary differentiation of rat tracheal epithelial cells, the cells were confluent and the quantity of produced and released mucin was highest on culture day 9, the mucin was mainly released to the apical side and tbe free $^3{H}$-glucosamine which was not incorporated to process of synthesis of mucin was left on the basolateral side. Taken together, we suggest that air-liquid interface culture system can be used as a substitute for immersion culture system and as an experimental model for in vivo mucus-hypersecretory diseases.

• 한국수정란이식학회지
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• 제14권3호
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• pp.163-169
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• 1999

This study was carried out to investigate the effect of co-culture system(bovine oviduct epithelial cells; BOEC) and defined culture system(modified TALP ; mTALP) on the development of IVM-IVF embryos, and survival of in vitro produced blastocysts after freezing and thawing. Occytes from the slaugheterhous ovaries were matured and fertilized using general protocol. The results obtained were as the following: 1. Survival rates of frozen-thawed blastocysts using 10% glycerol as cryoprotectant was higher in day 7 blastocysts than in Day 8 and 9 blastocysts from co-cultrue system, but survival rate of frozen-thawed blastocysts was higher in Day 10 blastocysts than in day 8 and 9 blastocysts from defined culture system. Regardless of their age, survival rate of frozen-thawed blastocysts was significantly higher (p<0.05) in co-culture system than in defined culture system. 2. The cell number of blastocysts was significanlty higher (p<0.05) in Day 7 blasotcysts than in Day 8 and 9 blastocysts from co-cultures, but the cell number of blsstocysts was significantly higher (p<0.05) in Day 10 blastocysts than in Day 8 and 9 blastocysts from defined culture system. Regardless of the culture system, blastocysts with higher cell number showed higher survival rates after freezing and thawing.

• Journal of the Korean Wood Science and Technology
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• 제33권1호통권129호
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• pp.63-76
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• 2005

목재문화는 시멘트문화를 대체하는 새로운 패러다임으로서, 목재문화가 학문적 체계를 갖추기 위하여는 공학적 연구만이 아니라 사회학적 연구도 병행되어야 한다. 본 연구는 아직 국내외적으로 전혀 시도된 바가 없는 목재문화의 사회학적 측면을 구조 기능적, 체계 기능적, 갈등적, 사회 변동적 측면에서 사회학적으로 연구하고 우리나라의 사례를 분석함으로써 목재문화의 사회학적 접근방법을 제시하는데 목적이 있다. 구조 기능적 측면에서 보면 사회체계는 최상위의 인식체계, 그 다음의 제도체계, 생활체계, 기술체계의 4가지 부분체계로 이루어진다. 이들 부분체계는 목재문화 전체의 기능을 유지하기 위하여 당위성 타당성 현실성 발전성의 기능을 발휘한다. 체계기능적 측면에서 볼 때 목재문화체계는 목재 효용성의 인지와 전파과정에 따라 개인적 체계와 조직적 체계, 이 두 가지를 망라하는 사회적 체계의 3가지로 구성되며, 갈등측면에서 볼 때 사회는 목재문화와 시멘트문화의 갈등양상에 따라 고유의 목재문화는 쇠퇴하고 시멘트문화가 확산되는 사회, 시멘트문화가 쇠퇴하고 목재문화가 확산되는 사회, 고유의 목재문화와 시멘트문화가 균형을 이루는 사회로 구분된다. 이와 같은 목재문화의 유형을 문화변동의 측면에서 분류하면, 목재문화에서 시멘트문화로의 변동, 시멘트문화에서 목재문화로의 변동, 목재문화 자체의 변동으로 분류할 수 있다. 또한 변동수준에 따라 분류하면 미시적 변동, 중간적 변동, 거시적 변동으로 분류된다. 이상과 같이 목재문화를 사회학적으로 조명하는 새로운 접근방법과 공학적 연구가 함께 진행되어 빠른 시일내에 학계에서 "목재문화론" 또는 "목재문화학"이 체계적으로 정립되기를 바란다.

• International journal of thyroidology
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• 제11권2호
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• pp.160-166
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• 2018

Background and objectives: Salivary hypofunction is one of the common side effects after radioiodine therapy, and its pathophysiology is salivary ductal stenosis resulting from ductal cell injury. This study aimed to develop the functional culture environment of human parotid gland ductal cells in in vitro three-dimensional perfusion culture system. Materials and Methods: We compared plastic dish culture method and three-dimensional culture system containing Matrigel and nanofiber. Morphogenesis of reconstituted salivary structures was assessed by histomorphometry. Functional characteristics were assessed by immunohistochemistry and reverse transcription polymerase chain reaction (aquaporin 5, CK7, CK18, connexin 43, and p21). In addition, we designed the media perfusion culture system and identified higher rate of cell proliferation and expression of connexin 43 in perfusion system comparing to dish. Results: Human parotid ductal cells were well proliferated with the ductal cell characters under environment with Matrigel. In the presence of Matrigel, aquaporin 5, CK18 and connexin 43 were more expressed than 2D dish and 3D nanofiber setting. In the media perfusion culture system, ductal cells in 3D culture media showed higher cells count and connexin 43 expression compared to 2D dish. Conclusion: This in vitro ductal cell perfusion culture system using Matrigel could be used to study for radioiodine induced sialadenitis model in vivo.

• 과학기술학연구
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• 제3권1호
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• pp.1-17
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• 2003

과학기술과 문화의 관계는 어떻게 이해해야 하는가? 과학기술은 문화의 일부로서 이해되는가 하면, 흔히 과학기술과 문화는 서로 배타적인 관계에 있는 것으로 주장되기도 한다. 이 논문에서는 먼저 과학기술을 문화의 한 형태로서 검토하였다. 분명히 과학은 인식적 가치를 창조하는 가치문화의 일부이며, 기술은 비록 도구적 문화로서 파악되는 경우에도 문화체계의 일부로 이해해야 한다. 그러므로 과학기술은 어느 정도의 '상대적 자율성'을 지닌 문화체계의 일부인 것이다. 다음으로 이 논문은 정치체계, 경제체계, 문화체계와 과학기술체계 간의 긴장관계에 대해 검토하였다. 특히 현대사회에서 정치와 경제체계에 의해 문화체계가 식민화되는 과정에서 과학기술체계의 도구적 합리성의 왜곡에 의해서 야기된 기술지배의 부정적 결과들에 대해 검토하고, 이를 극복하기 위해서는 도구적 합리성이 사회적 합리성과 의사소통합리성 등에 의해 균형을 이루어야 함을 지적하였다. 끝으로 이 논문에서는 정치, 경제, 문화 및 시민사회 각각의 '과학문화'의 특성과 그와 같은 과학적 부분문화(scientific subcultures)들 간의 역동적인 관계와 사회과정을 통해 '인간화되고 민주적인 과학문화'가 형성될 수 있는 가능성에 대해 논의하였다.

• KSBB Journal
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• 제13권4호
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• pp.411-417
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• 1998

The possibility of application of membrane type immobilized adsorbent to the fed-batch or perfusion culture system with anchorage-independent cells as well as batch system was investigated. The improvement in cell density and cell viability due to the combination of immobilized adsorbent with each culture system was evaluated for the investigation, and the optimum culture system employing immobilized adsorbent system was suggested based on the results. It was observed that the system with immobilized adsorbent showed better cell growth and cell viability than that without immobilized adsorbent in every operation system of batch, fed-batch, and perfusion. In case of batch system, 200% improvement of maximum cell density was observed in the system where ammonium chloride was added on purpose. And 50% improvement of maximum cell density was observed in the fed-batch system where ammonium ion accumulates significantly, while small increase in maximum cell density was observed in the perfusion system where dilution of waste byproducts exists. Especially, the fed-batch system showed the most significant improvement on cell growth because both compensation of nutrient and removal of ammonium ion occurred simultaneously in the system. Therefore a combined system of immobilized adsorbent and fed-batch operation could be suggested as an optimum system with in situ removal of ammonium ion.

• 한국잠사곤충학회지
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• 제38권2호
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• pp.150-153
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• 1996

1차 액체배양액과 2차 pellet 배지로 이루어지는 대량배양체계의 다른 곤충병원성 곰팡이인 Beauveria bassiana, Beauveria brongniartii, Metarhizum anisopliae, Verticillium lecanii 등에 대한 효용성을 조사하기 위하여 단균사 및 분생포자의 성장율을 비교하였다. 그 결과, 액체배양액에서 단균사의 성장율은 접종 후 72시간에 그 수가 최초 접종수에 비해 103-104배까지 증가함을 확인할 수 있었으며, pellet 배지에서 분생자의 수는 접종 후 3주 째에 103배까지 증가됨을 확인할 수 있었다. 본 실험의 결과 진균의 대량배양을 위해 선발된 1차 액체배양액과 2차 pellet 배지는 여러 곤충병원성 진균의 성장에 효율적인 체계임을 확인할 수 있었다.