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Studies on the Air-Liquid Interface Culture as an Experimental Model for Physiology and Pharmacology of Tracheal Epithelial Cells  

이충재 (충남대학교 의과대학 약리학 교실)
이재흔 (충남대학교 의과대학 약리학 교실)
석정호 (충남대학교 의과대학 약리학 교실)
허강민 (충남대학교 의과대학 약리학 교실)
Publication Information
Biomolecules & Therapeutics / v.10, no.4, 2002 , pp. 281-286 More about this Journal
Abstract
In this study, we intended to get a preliminary data for establishing rat tracheal surface epithelial(RTSE) cell culture system as an experimental model for physiology and pharmacology of tracheal epithelial cells. Primary culture on the membrane support and application of the air-liquid interface system at the level of cell layer were performed. The cell growth rate and mucin production rate were measured according to the days in culture. The results were as follows: this culture system was found to manifest mucocilliary differentiation of rat tracheal epithelial cells, the cells were confluent and the quantity of produced and released mucin was highest on culture day 9, the mucin was mainly released to the apical side and tbe free $^3{H}$-glucosamine which was not incorporated to process of synthesis of mucin was left on the basolateral side. Taken together, we suggest that air-liquid interface culture system can be used as a substitute for immersion culture system and as an experimental model for in vivo mucus-hypersecretory diseases.
Keywords
tracheal surface epithelial cell; air-liquid interface culture; mucin-hypersecretory diseases;
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