International journal of thyroidology

Korean Thyroid Association (대한갑상선학회)
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Development of the Three-Dimensional Perfusion Culture Technology for the Salivary Ductal Cells

타액선 도관세포의 관류 배양 기술 개발

  • Kim, Ji Won (Department of Otolaryngology, Inha University School of Medicine) ;
  • Kim, Jeong Mi (Department of Otolaryngology, Inha University School of Medicine) ;
  • Choi, Jeong-Seok (Department of Otolaryngology, Inha University School of Medicine)
  • 김지원 (인하대학교 의과대학 이비인후과학교실) ;
  • 김정미 (인하대학교 의과대학 이비인후과학교실) ;
  • 최정석 (인하대학교 의과대학 이비인후과학교실)
  • Received : 2018.04.17
  • Accepted : 2018.05.24
  • Published : 2018.11.30
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Abstract

Background and objectives: Salivary hypofunction is one of the common side effects after radioiodine therapy, and its pathophysiology is salivary ductal stenosis resulting from ductal cell injury. This study aimed to develop the functional culture environment of human parotid gland ductal cells in in vitro three-dimensional perfusion culture system. Materials and Methods: We compared plastic dish culture method and three-dimensional culture system containing Matrigel and nanofiber. Morphogenesis of reconstituted salivary structures was assessed by histomorphometry. Functional characteristics were assessed by immunohistochemistry and reverse transcription polymerase chain reaction (aquaporin 5, CK7, CK18, connexin 43, and p21). In addition, we designed the media perfusion culture system and identified higher rate of cell proliferation and expression of connexin 43 in perfusion system comparing to dish. Results: Human parotid ductal cells were well proliferated with the ductal cell characters under environment with Matrigel. In the presence of Matrigel, aquaporin 5, CK18 and connexin 43 were more expressed than 2D dish and 3D nanofiber setting. In the media perfusion culture system, ductal cells in 3D culture media showed higher cells count and connexin 43 expression compared to 2D dish. Conclusion: This in vitro ductal cell perfusion culture system using Matrigel could be used to study for radioiodine induced sialadenitis model in vivo.

Keywords

References

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