• Journal of Plant Biology
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• 제33권4호
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• pp.325-332
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• 1990

Light membrane vesicles were isolated from the zucchini hypocotyl by floatation on ficoll density gradients and the proteins were solubilized with Triton X100. Three ATP-hydrolyzing enzymes were partially purified by ion-exchange and gel filtration chromatography and isoelectric focusing. There are plasma membrane-type ATPase whose activity was inhibited by vanadate but not by nitrate, tonoplast-type ATPase which was sensitive to nitrate but insensitive to vanadate and one having a phosphatase activity with a pI value different from that of an acid phosphatase. A fraction was obtained after DEAE-ion-exchange chromatography crossreacting with polyclonal antibodies against Ca2+ -ATPase from human erythrocytes.

• 식물병연구
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• 제22권3호
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• pp.133-144
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• 2016

Powdery mildew (Podosphaera xanthii) causes severe damage to pumpkin crops grown in open fields and plastic house. Initially, we used ten accessions of pumpkin species; Cucurbita pepo (3), C. maxima (2), C. moschata (2), and C. argyrosperma (3) to study the disease progress in different stages of seedling development. Commercial pumpkin varieties were used as susceptible host for inoculum management and young seedlings were inoculated by dusting powdery mildew spores on the cotyledons, 1-true-leaf and 2-true-leaf seedling stages. Based on initial study, we further evaluated 218 pumpkinaccessions for their resistance to powdery mildew in different seedling stages under plastic house. Area under disease progress curve (AUDPC) and relative AUDPC (rAUDPC) was higher in cotyledonary and 1-true-leaf than 2-true-leaf stage. Seedlings at cotyledons and 1-true-leaf seedling stage displayed more susceptibility to powdery mildew. Based on evaluation of 2-true-leaf stage, IT 110859 and IT 278459 from C. pepo and C. argyrosperma identified as resistant (<0.2). Of the 228 pumpkin accessions, 21 (9.2%) pumpkin accessions consisting of C. pepo (2), C. maxima (5), C. moschata (13), and C. argyrosperma (1) exhibited intermediate resistance (<0.4) to powdery mildew and these accessions are useful to growers for its rational management.

• 한국식품위생안전성학회지
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• 제24권2호
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• pp.124-127
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• 2009

기능성 식품 소재로 주목을 받고 있는 호박종자추출물의 에스트로겐 활성을 재조합 효모를 이용하여 측정하였다. 양성대조물질로 사용된 $17{\beta}-Estradiol$은 강력한 에스트로겐 활성을 나타내었으며, 농도의존적인 반응을 나타내었다. 초임계추출법에 의하여 얻어진 호박종자추출물 분말을 에탄올로 재추출하여 시험에 사용한 결과 에스트로겐 활성이 관찰되지 않았다. 따라서 호박종자추출물이 건강기능식품 제조에 사용될 때 에스트로겐 활성으로 인한 부작용은 없을 것으로 기대된다.

• 한국응용곤충학회지
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• 제20권4호
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• pp.191-195
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• 1981

모자익병징을 나타내는 호박, 박, 오이, 참외, 수박의 박과작물에서 Watermelon Mosaic Virus(WMV)를 분리동정하였다. 이병주로 부터 분리된 WMV는 복숭아혹진딧물(Myzus persicae, Sulzer)에 의해 쉽게 충매전염되었고 종자전염은 되지 않았다. 지표식물검정결과 명아주에서는 접종엽에 대형의 국부병반이, 호박, 박, 오이, 참외, 수박에서는 접종상엽에서 모자익병반이 나타났고 담배(Nicotiana tabacum, Bright yellow), 담배 (Nicotanan glutinosa), 동부, 페추니아, 독말풀에서는 반응이 나타나지 않았다. WMV isolate의 물리적 성질은 내열성 $55\~65^{\circ}C$, 내희석성 $10^{-4}\~10^{-5}$배, 내보존성 $7\~8$일간이었다. WMV isolate의 항혈청반응은 미량침강법에서 양성으로 나타났고 바이러스립자의 형태는 전자현미경검경결과 대부분이 750nm의 사상입자이었다. 이상의 시험결과로 박과 작물에서 분리한 바이러스가 WMV 임이 확인되었다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 임시총회 및 추계 학술발표회
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• pp.72.1-72
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• 1999

• The Plant Pathology Journal
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• 제29권3호
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• pp.285-293
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• 2013

Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ~1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ~580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere.

• 생명과학회지
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• 제13권5호
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• pp.590-595
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• 2003

The objective of this study was to assess of genetic variation within and between pumpkin species including Cucurbita maxima, C. moschata, C. pepo and C. maxima${\times}$C. moschata using RAPD markers. The 16 primers showed the amplification of 136 scorable fragments ranging from about 100 bp to 2300 bp. A total of 94 DNA fragments were polymorphic with an average 5.9 polymorphic bands per primer. A species $(C. maxima\timesC. moschata)$ has the highest number of polymorphic loci. Based on obtained data, UPGMA cluster analysis was conducted. Twenty pumpkin cultivars were classified into three large categories and identified genetic distance of cluster ranging from 0.38 and 1.00. Clustering was in accordance with the division of Curcurbitaceae into four species, C. maxima, C. moschata, C. pepo and C. $C. maxima\timesC. moschata$. Therefore, RAPD method may be essential tool for enabling discrimination of pumpkin cultivars.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 임시총회 및 추계 학술발표회
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• pp.82.1-82
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• 1999

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 임시총회 및 추계 학술발표회
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• pp.86.1-86
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• 1999

• 식물병연구
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• 제14권3호
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• pp.229-232
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• 2008

모자이크 증상의 과꽃(Callistephus chinensis L.)으로부터 Cucumber mosaic virus (CMV)의 한 계통(Cas-CMV)를 분리하고, Fny-CMV와 As-CMV를 대조로 기주반응, dsRNA, RT-PCR 및 RFLP분석을 통하여 바이러스를 동정하였다. Cas-CMV의 특징적인 기주반응의 차이는 박과 식물에서 발현되는 강한 병정이었으며, 특히 쥬키니호박에 접종하였을 때에는 접종 15-20일 후에 심한 모자이크 증상과 함께 어린 식물이 고사되는 괴저현상을 나타냈다. DsRNA분석과 RT-PCR실험의 결과는 Cas-CMV가 서브그룹 I의 CMV에 속하는 것으로 나타났으며, 더욱이 HindIII를 이용한 RFLP 분석은 Cas-CMV가 서브그룹 IA 구분되었다.