Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Screening of Pumpkin (Cucurbita spp.) Germplasm for Resistance to Powdery Mildew at Various Stages of Seedlings Growth

  • Luitel, Binod Prasad (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Sang Gyu (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Sung, Jung Sook (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hur, On-Sook (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yoon, Mun-Sup (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Rhee, Ju-Hee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Baek, Hyung-Jin (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ryu, Kyoung-Yul (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ko, Ho-Cheol (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2016.04.14
  • Accepted : 2016.07.29
  • Published : 2016.09.30
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Abstract

Powdery mildew (Podosphaera xanthii) causes severe damage to pumpkin crops grown in open fields and plastic house. Initially, we used ten accessions of pumpkin species; Cucurbita pepo (3), C. maxima (2), C. moschata (2), and C. argyrosperma (3) to study the disease progress in different stages of seedling development. Commercial pumpkin varieties were used as susceptible host for inoculum management and young seedlings were inoculated by dusting powdery mildew spores on the cotyledons, 1-true-leaf and 2-true-leaf seedling stages. Based on initial study, we further evaluated 218 pumpkinaccessions for their resistance to powdery mildew in different seedling stages under plastic house. Area under disease progress curve (AUDPC) and relative AUDPC (rAUDPC) was higher in cotyledonary and 1-true-leaf than 2-true-leaf stage. Seedlings at cotyledons and 1-true-leaf seedling stage displayed more susceptibility to powdery mildew. Based on evaluation of 2-true-leaf stage, IT 110859 and IT 278459 from C. pepo and C. argyrosperma identified as resistant (<0.2). Of the 228 pumpkin accessions, 21 (9.2%) pumpkin accessions consisting of C. pepo (2), C. maxima (5), C. moschata (13), and C. argyrosperma (1) exhibited intermediate resistance (<0.4) to powdery mildew and these accessions are useful to growers for its rational management.

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References

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