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http://dx.doi.org/10.5352/JLS.2003.13.5.590

Assessment of Genetic Relationship among Curcurbitaceae Cultivars Revealed by RAPD Marker  

김창훈 (농림부 국립종자관리소 재배시험과)
이승인 (농림부 국립종자관리소 재배시험과)
유병천 (농림부 국립종자관리소 재배시험과)
송인호 (농림부 국립종자관리소 재배시험과)
권용삼 (농림부 국립종자관리소 재배시험과)
Publication Information
Journal of Life Science / v.13, no.5, 2003 , pp. 590-595 More about this Journal
Abstract
The objective of this study was to assess of genetic variation within and between pumpkin species including Cucurbita maxima, C. moschata, C. pepo and C. maxima${\times}$C. moschata using RAPD markers. The 16 primers showed the amplification of 136 scorable fragments ranging from about 100 bp to 2300 bp. A total of 94 DNA fragments were polymorphic with an average 5.9 polymorphic bands per primer. A species $(C. maxima\timesC. moschata)$ has the highest number of polymorphic loci. Based on obtained data, UPGMA cluster analysis was conducted. Twenty pumpkin cultivars were classified into three large categories and identified genetic distance of cluster ranging from 0.38 and 1.00. Clustering was in accordance with the division of Curcurbitaceae into four species, C. maxima, C. moschata, C. pepo and C. $C. maxima\timesC. moschata$. Therefore, RAPD method may be essential tool for enabling discrimination of pumpkin cultivars.
Keywords
Cucurbitaceae; Genetic similarity; RAPD; Cluster analysis;
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  • Reference
1 Genetic variability in pumpkin (Cucurbita maxima) using RAPD markers /
[ Ferriol,M.;B.Pico;F.Nuez ] / Cucurbit genetics Coop
2 /
[ Sneath,P.H.A.;R.R.Sokal ] / Numeical taxonomy
3 DNA profiling and plant variety registration. Ⅲ: The statistical assessment of distinctness in wheat using amplified fragment length polymorphisms /
[ Law,J.R.;P.Donini;R.M.D.Koebner;J.C.Reeves;R.J.Cooke ] / Euphytica   DOI   ScienceOn
4 Genetic diversity within and between the species Cucurbita pepo, C. moschata and C. maxima as revealed by RAPD markers /
[ Barnek,M.;G.Stift;J.Vollmann;T.Lelley ] / Cucurbit genetics Coop. Rpt.
5 Applicability of molecular markers in the context of protection of new vaieties of cucumber /
[ Bernet,G.P.;S.Bramardi;D.Calvache;E.A.Carbonell;M.J.Asins ] / Plant Breeding
6 Assessment of genetic relationships in Cucurbita pepo (Cucurbitaceae) using DNA markers /
[ Paris,H.S.;N.Yonash;V.Portnoy;N.Mozes-Daube;G.Tzuri;N.Katzir ] / Theor Appl Genet
7 Discordant patterns of allozyme and morphological variation in Mexican Cucurbita /
[ Wilson,H.D. ] / Systematic Botany   DOI   ScienceOn
8 Further ISSR and preliminary SSR analysis of relationships among accessions of Cucurbita pepo /
[ Katzir,N.;Y.Tadmor;G.Tzuri;E.Leshzeshen;N.Mozes-Daube;Y.Danin-Poleg;H.S.Paris ] / Acta Hort
9 The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties /
[ Tommasini,L.;J.Batley;G.M.Arnold;R.J.Cooke;P.Donini;D.Lee;J.R.Law;C.Lowe;C.Moule;M.Trick;K.J.Edwards ] / Theor Appl Genet
10 Identification of cucurbitaceae culitivars by using RAPD markers /
[ Jeon,H.J.;C.G.Been;K.H.Hong;Y.H.Om;B.D.Kim ] / J. Kor Soc Hort Sci
11 Molecular markers in variety and seed testing /
[ Donini,P.;R.J.Cooke;J.C.Reeves ] / Plant Genetic Engineering : towards the third millennium
12 Diversity in freeliving populations of Cucurbita pepo (Cucubataceae) as assessed by random amplified polymorphic DNA /
[ Decker-Walters,D.S.;J.E.Staub;S.M.Chung;E.Nakata;H.D.Quemada ] / Systematic Botany