• 제목/요약/키워드: Cucumis sativus

검색결과 188건 처리시간 0.019초

Cucumber (Cucumis sativus L.) Fruit and Combination with Losartan Attenuate the Elevation of Blood Pressure in Hypertensive Rats Induced by Angiotensin II

  • Tomi Hendrayana;Klaudia Yoana;I Ketut Adnyana;Elin Yulinah Sukandar
    • 대한약침학회지
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    • 제26권4호
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    • pp.298-306
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    • 2023
  • Objectives: Cucumis sativus L. (C. sativus) is vegetable commonly used for managing blood pressure and often consumed in combination with standard antihypertensive therapy, despite lack of scientific evidence supporting their use. Combination of herbs and standard medication could have positive or negative effects. Therefore, this study aimed to evaluate the antihypertensive activity of C. sativus and the combined effect with losartan in the hypertensive rat model induced by angiotensin II. Angiotensin II is a component of the renin-angiotensin-aldosterone system that, upon binding to its receptor, constricts blood vessels leading to elevation of blood pressure. Methods: In an antihypertensive study, rats received C. sativus orally at doses of 9, 18, 27, and 36 mg/kg (full dose); while in a combination study, animals received losartan 2.25 mg/kg combined by either with C. sativus 9 or 18 mg/kg. The standards group received losartan 2.25 mg/kg or 4.5 mg/kg (full dose). Results: Blood pressure was measured using the tail-cuff method. C. sativus significantly attenuated angiotensin II-induced hypertension as observed in groups receiving C. sativus at 9, 18, 27, and 36 mg/kg at 30 minutes after induction showed the average change (Δ) of systolic blood pressure (SBP) and diastolic blood pressure (DBP) with respect to time zero were 28.8/18.3, 24.8/15.8, 22.8/15.5, and 11.5/9.0 mmHg, respectively. Whereas the average change (Δ) of SBP and DBP in the rats receiving the combination of half doses of C. sativus and losartan were 8.8/9.0 mmHg, respectively. These diminished effects were better than a full dose of C. sativus and comparable with a full dose of losartan (6.5/7.8 mmHg). Conclusion: The present findings indicate that C. sativus dose-dependently blocks blood pressure elevation induced by angiotensin II. The combination of half dose of C. sativus and losartan has an additive effect in lowering blood pressure.

아연, 산화 아연 나노 입자가 오이에 미치는 독성 및 생물이용성 (Phytotoxicity and Bioavailability of Zinc, Zinc Oxide Nanoparticles to the Cucumis sativus)

  • 송이레;남윤선;이인숙
    • 대한환경공학회지
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    • 제31권7호
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    • pp.467-472
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    • 2009
  • Seedling 성장 실험은 나노입자에 대한 독성 평가로 사용되어 왔다. 본 연구에서는 오이에 대한 나노 아연과 나노 산화아연의 독성을 filter paper방법과 phytagel방법을 통해 평가해 보았다. 비교 결과 phytagel방법이 불용성인 나노아연, 산화 아연의 침전을 방지했다. 오이 seedling 길이는 노출된 나노 아연, 나노 산화 아연의 농도에 따라 filter 방법에서 음의 관계를 나타냈다. 나노 아연과 나노 산화 아연에 대한 오이의 EC50값은 598, 600 mg/L로 나타났다. Filter paper방법에서만 아연의 오이 내 생체 축적량은 노출된 나노 아연, 나노 산화 아연의 농도에 따라 증가하였다. 관찰 결과 filter paper방법이 phytagel 방법보다 더 좋은 protocol로 나타났다

Plant Assay에 의한 비소오염 토양평가

  • 안윤주;이주영;임승윤;정지영;정혜원
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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    • pp.198-200
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    • 2004
  • Four crop plant species were tested to assess an ecotoxicity in arsenic-amended soils. Test plants were Sorghum bicolor, Cucumis sativus, Triticum aestivum, and Phaseolus radiatus. The presence of arsenic decreased the root and shoot growths. Arsenite was more toxic than arsenate to all test plants. Root growths of Phaseolus radiatus and Cucumis sativus seem to be a good protocol to assess ecotoxicity of soils contaminted by arsenic.

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형질전환 오이(Cucumis sativus L.) 식물체에서 완두 Superoxide Dismutase 유전자의 발현 (Expression of Pea Superoxide Dismutase Gene in Transgenic Cucumber (Cucumis sativus L.) Plants)

  • 김재훈;오승용;이행순;조만현;이은모;우인식;곽상수
    • 식물조직배양학회지
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    • 제25권3호
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    • pp.201-206
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    • 1998
  • Superoxide dismutase (SOD)를 많이 생산하는 신기능성 오이(Cucumis sativus L.) 과실을 개발하기 위하여, 발아 7일째의 자엽절편에 완두콩(Pisum sativum) 유래 MnSOD 유전자를 Agrobacterium tumefeciens LBA4404의 매개로 형질 전환시켰다. 1mg/L zeatin, 0.1mg/L IAA, 300mg/L claforan, 100mg/L kanamycin이 포함된 선발배지에서 부정아를 유도한 후, 1주일 간격으로 계대배양하면서 kanamycin 저항성 형질전환체를 선발하였다. 선발배지 배양 6주 후 kanamycin 저항성을 가진 부정아만을 절단하여 0.2mg/L NAA가 함유된 발근배지에 옮겨 뿌리를 유도한 후, 화분에서 순화시켰다. 소식물체의 잎에서 DNA를 분리하여 PCR 로 분석한 결과, 3개의 식물체에서 선발마커유전자인 NPTII 유전자가 존재함을 확인하여 SOD 유전자가 오이에 도입됨을 확인할 수 있었다. 이중 한 개체의 과실에서 SOD 함량이 무처리개체에 비해 약 3.2배 높았으며 native gel 전기영동에서 도입한 SOD isoenzyme 밴드가 강하게 검출되었다.

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생태독성학적 기법을 이용한 토양오염평가 방안 (Soil Pollution Assessment Based on Ecotoxicological Methods)

  • 안윤주;정승우
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제10권6호
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    • pp.56-62
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    • 2005
  • 본 연구는 물리화학적인 평가방법에만 의존하고 있는 토양오염평가에 대해 생태독성학적 방법(ecotoxicological methods)을 도입하긴 위한 방안을 제시하였다. 오염물질에 민감하게 반응하는 토양생물을 이용하여 단기간에 결과를 얻을 수 있도록 측정방법을 고안하고, 대상토양에서의 생존 및 성장률을 정량적인 방법으로 측정하여 토양의 안전성 및 오염정도를 평가하였다. 실험연구는 토양 동$cdot$식물 모두를 포함하기 위한 노력의 일환으로 식물독성 실험(발아 및 성장저해실험), 그리고 토양 무척추동물 독성실험(단순접촉법에 의한 생존률 실험)으로 구성되었다 식물독성실험에서는 국내 주요농작물인 Zea may, Triticum aestivum, Cucumis sativus, 그리고 Sorghum bicolor를 대상 식물로 하여 중금속 토양의 오염정도와 식물의 생장률, 식물 내 중금속 축적량의 상관관계를 분석하고. 이들 실험식물 중에 꼭 토양오염의 평가에 적합한 식물지표종으로 Cucumis sativus와 Sorghum bicolor를 제안하였다. 토양무척추동물 독성실험에서는 국내사육종일. Perionyx excavatus를 이용하여 톨루엔오염토양을 평가하였다. 본 연구에서 제시한 토양 생물을 이용하는 생태독성학적 평가방법은 물리화학적 측정방법과 함께 토양오염을 평가하는 정량적인 방법으로 사용될 수 있을 것으로 판단된다.

High Frequency Shoot Regeneration from leaf Explants of Cucumber

  • Seo, Seung-Hee;Bai, Dong-Gyu;Park, Hyeon-Yong
    • Journal of Plant Biotechnology
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    • 제2권1호
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    • pp.51-54
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    • 2000
  • Leaf explants of the cucumber (Cucumis sativus L.) were cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of $\alpha$-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). Direct shoot orgnogenesis as well as callus formation with somatic embryos and multiple shoots was observed from leaf explants of cvs. Shinhukjinju and Chungjang. The highest frequency of shoot formation 80% was observed on MS medium supplemented with NAA/BAP (5.0 ${\mu}{\textrm}{m}$/2.5 ${\mu}{\textrm}{m}$), with explants forming 3-7 shoots. Shoots formation occured within 3 to 4 weeks. Only one subculture of calli was required for plant regeneration on normal growth regulator-free medium. Plantlets transferred to soil developed into plants of normal appearance, which flowered and set fruits.

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Assessment of Factors Influencing Agrobacterium Mediated Transformation in Cucumber (Cucumis sativus L)

  • Sureshkumar P.;Selvaraj N.;Ganapathi A.;Kasthurirengan S.;Vasudevan A.;Anbazhagan V. Ramesh
    • Journal of Plant Biotechnology
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    • 제7권4호
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    • pp.225-231
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    • 2005
  • Five day old cotyledon explants of Cucumber (Cucumis sativus L) cv Poinsett 76 were cocultivated with two Agrobacterium strains (EHA105 and LBA 4404) each carrying GUS as the reporter gene and npt-II as the selection marker gene in the T-DNA region of the vector. Transformed shoots were selected at 150 mg/L kanamycin. A two day cocultivation coupled with $20\;{\mu}M$ acetosyringone increased the frequency (8.2 and 15.4 shoots) of GUS expression in the shoots of transformed plant. Among the two Agrobacterium strains, EHA 105 performed better than LBA 4404 in bringing two-fold increase in transformation efficiency (14%) than LBA 4404 (7.4%). PCR analysis was done to confirm the integration of T-DNA into cucumber genome.

Bioluminescent Assay of ${\alpha}$-Oxidase from Cucumis sativus using Bacterial Luciferase-Coupled Reaction

  • Cho, Ki-Woong
    • BMB Reports
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    • 제33권4호
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    • pp.353-357
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    • 2000
  • A new assay method of ${\alpha}-Oxidase$ (fatty acid : oxygen dioxygenase, 1-decarboxylating) was developed using a bioluminescence reaction system of marine luminous bacterium, Photobacterium phosphoreum. ${\alpha}$-Oxidase was isolated from a cucumber (Cucumis sativus). Pentadecanoic acid was used as a substrate, and the product, tetradecanal, was analyzed with a bacterial luciferase-coupled reaction. Initial light intensity was directly related to the concentration of tetradecanal in the range of 1 nM to 10 ${\mu}M$. Optimal pH and temperature were 7.5 and $25^{\circ}C$, respectively. Optimal pentadecanoic acid concentration in a standard assay of ${\alpha}$-oxidase was 0.1 mM. The Km value of pentedecanoic acid was $85{\mu}M$. This method is straightforward, rapid, convenient, and easy. Its needs no treatment or extraction of reaction mixture.

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Some Trace Metals and their Ratios in Aloe (Aloe vera L.), Cucumber (Cucumis sativus L. var. tuberculatus Gabaj.) and Sponge-gourd (Luffa cylindrica L.) in Korea

  • Lee, Chang-Jun;Park, Jung-Sang;Jang, Gi-Chul;Kim, Sang-Deog A.
    • 한국자원식물학회지
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    • 제22권6호
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    • pp.540-545
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    • 2009
  • Aloe (Aloe vera L.), cucumber (Cucumis sativus L. var. tuberculatus Gabaj.) and sponge.gourd (Luffa cylindrica L.) are well utilized in Asian countries as traditional medicines and cosmetics, or foods. And we carried out an experiment to see if there are some specific ratios among trace metals on the plants. Though the metal concentrations varied significantly depending on the sampling periods, aloe gel is high in iron (Fe) and calcium (Ca). Cucumber fruit juice is also high in iron (Fe) and potassium (K). Sponge.gourd sap contains a high amount of zinc (Zn) and calcium (Ca). The uses of the previous 3 plant parts are considered to have some relations to their different ratios and of their trace metals.

박과 작물 4종의 핵형분석 (Chromosome Compositions of Four Cultivated Cucurbitaceae Species.)

  • 권지연;박혜미;이성남;최선희;송경아;김현희
    • 생명과학회지
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    • 제18권7호
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    • pp.1019-1022
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    • 2008
  • 박과 작물 4종(오이, 수박, 참외, 수세미)에 대한 핵형분석 연구를 수행하였다. 염색체 조성은 모두 이배체로 염색체 수와 형태적 특징에 있어서 종에 따라 차이를 나타냈다. 오이의 핵형은 2n=14=14m, 수박은 2n=22=22m으로 2번 염색체가 부수체를 지니며, 참외는 2n=24=18m+6sm으로 7번 염색체가 부수체를 지니고 있었다. 수세미는 2n=26=26m으로 1번 염색체가 부수체를 지니고 있었다. 핵형분석 결과 종에 따라 특징적인 염색체 수와 조정을 나타내었으며 종간 게놈 구성의 정보에 따른 박과 작물 육종화 작업의 기초 자료를 제공할 수 있으리라 본다.