• Title/Summary/Keyword: Cu 스트레스

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Alterations of Glutathione Peroxidase Patterns by Stressor Treatment in Rice Seedling Roots (스트레스 물질에 의한 벼 glutathione peroxidase 활성패턴 변화)

  • Kim, Yoon-Kyoung;Lee, Mi-Young
    • Applied Biological Chemistry
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    • v.48 no.1
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    • pp.53-59
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    • 2005
  • The effect of various stressors such as reductant ascorbic acid, signalling molecules (salicylic acid and methyl jasmonic acid), heavy metals $(NiCl_2,\;and\;MnSO_4)$ and NaCl on the glutathione peroxidase (GPX) activities and isoenzyme expression patterns were investigated in rice seedling roots. Total GPX activity increased according to the increase of ascorbic acid concentration. Prominent enhancement of GPX1 isozyme due to ascorbic acid contributed to the increase of total GPX activity. GPX showed different reactivity toward salicylic acid and methyl jasmonic acid. GPX activity increased at 0.1 mM salicylic acid, and then decreased thereafter. However, GPX increased gradually in a methyl jasmonic acid concentration-dependent manner, and 3 fold increase of GPX activity was found at 1 mM methyl jasmonic acid. Moreover, GPX1 isozyme increased according to the increase of salicylic acid, while GPX1 isozyme decreased according to the increase of methyl jasmonic acid. When metal ions were treated, GPX activity increased considerably according to the increase of $NiCl_2$ concentration, however, GPX activity increased about 2 fold at 0.5 mM $CuSO_4$ and then decreased. Enhancement of GPX1 isozyme contributed to the increase of total GPX activities in $NiCl_2-treated$ and $MnSO_4-treated$ rice seedlings. Total GPX activity increased 1.7 fold in response to 300 mM NaCl. Especially GPX2 isozyme showed gradual increase according to the increase of NaCl concentration.

Effects of Fermented Mulberry Leaves (Morus alba L.) on Oxidative Modification of Antioxidnat Enzymes (항산화 효소의 산화적 변형에 뽕잎 발효물이 미치는 영향)

  • Kang, Jung Hoon
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.3
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    • pp.985-994
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    • 2019
  • Muberry (Morus alba L.) leaves fermented with Hericium erinaceum mycelium (MA-HE) were assessed for the protection against oxidative modification of antioxidant enzymes, Cu,Zn-superoxide dismutase(SOD) and ceruloplasmin(CP). MA-HE were shown to significantly inhibited oxidative modifications and inactivations of Cu,Zn-SOD and CP induced by peroxyl radical. Antioxidant activity of MA-HE evaluated using peroxyl radical scavenging assays. MA-HE showed 44.03% of peroxyl radical scavenging activity at $100{\mu}g/mL$. Thus, MA-HE protect the antioxidant enzymes from oxidative damage by the scavenging peroxyl radicals. The results suggested that MA-HE was effectively removed reactive oxygen species in cells, thereby protecting cytotoxicity caused by oxidative stress.

중금속 4종 (Hg, Cd, Cu, Pb)에 대한 넙치(Paralichthys olivaceus) 아가미의 조직학적 반응 특이성

  • Lee, Jung-Sik;Jeong, Seon-Yeong;Kang, Ju-Chan;Lee, Jung-A;Jin, Pyung
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.534-535
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    • 2001
  • 수서동물의 아가미 형태 및 구조적 변화에 미치는 독성물질 또는 물리적인 스트레스의 영향에 관해서는 카드abf(You et al., 1978), 니켈, 크롬, 카드뮴(Hughes., 1979), 살충제(ojha et al., 1989), pH (Munshi and Singh, 1992), 염분농도(Kim et al., 1993; Moon, 1995), formalin (Ryu et al., 1998), 철 (Kang et al., 1999)등 많은 연구가 보고되고 있다. 하지만 이러한 연구 보고에서 나타난 대부분의 구조적 변화는 비특이적인 현상이지만, 스트레스 노출 조간과 무관한 것은 아니다 (Mallatt, 1985). (중략)

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Variation of Carbonization Pattern and Crystal Structure of Polyvinyl Chloride Wire Under the Thermal Stresses (열 스트레스에 의한 비닐절연전선의 탄화 패턴 및 결정 구조의 변화)

  • Choi, Chung-Seog;Kim, Hyang-Kon
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.57 no.3
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    • pp.332-337
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    • 2008
  • We analyzed carbonization pattern and crystal structure of polyvinyl chloride wire by thermal stress. Copper that is oxidized at normal temperature is a reddish brown. If under the thermal stress range of 500 to 700 [$^{\circ}C$], carbonization and exfoliation occurrence. Section structure of electric wire is same as arrangement of particle in metallograph analysis. But, as thermal stress increases, size of particle is enlarged. Electric wire displays elongation structure in SEM image analysis and elongation structure collapses when receive thermal stress at 300 [$^{\circ}C$]. In EDX analysis, we get the spectra of CuL, CuK, OK, and ClK. FT-IR analysis was shown new spectra with in range of $1,440{\sim}1,430\;[cm^{-1}]$, 1,340 [$cm^{-1}$], 1,240 [$cm^{-1}$].

Growth and Annealing Effect of Cu thin Films Using Electroplating Technique (전해도금법을 이용한 구리 박막의 성장 및 열처리 효과)

  • 박병남;강현재;최시영
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.40 no.10
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    • pp.1-8
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    • 2003
  • Copper thin films were deposited on a Cu/Ta/Si substrate using the electroplating technique. Deposition rate was about 200 nm/min in proportion to current density and in inverse proportion to flow rate. Resistivity of copper thin film was approximately 2.1 ${\mu}$Ωcm and Int$\sub$(111)//Int$\sub$(200)/ ratio of copper film was 5.4 and no significant impurities were detected. After the deposition, electroplating copper films were annealed at various temperatures in a background pressure of 10$\^$-3/ torr. The resistivity of copper thin films were improved by ∼17 % and texture was improved by ∼40 % after annealing at 170$^{\circ}C$. The stress in films was not reduced much after annealing below 170$^{\circ}C$.

Gene Structure and Altered mRNA Expression of Metallothionein in Response to Metal Exposure and Thermal Stress in Miho Spine Loach Cobitis choii (Cobitidae; Cypriniformes) (미호종개 metallothionein 유전자의 구조 및 중금속 노출과 고온 자극에 대한 MT mRNA의 발현 특징 분석)

  • Lee, Sang-Yoon;Nam, Yoon-Kwon
    • Korean Journal of Ichthyology
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    • v.23 no.1
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    • pp.61-69
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    • 2011
  • Gene and promoter structures of metallothionein(MT) from Miho spine loach (Cobitis choii; Cypriniformes) were characterized, and the transcriptional responses to experimental exposures to heavy metals and heat stress were examined. The C. choii metallothionein displayed well-conserved features of teleostean metallothioneins at gDNA, mRNA and amino acid levels. Bioinformatic analysis predicted that the C. choii MT regulatory region potentially possessed various motifs or elements targeted by various transcription factors associated with metal-coordinating regulation (e.g., metal transcription factor-1), immune responses (e.g., nuclear factor kappa B), and thermal modulations (e.g., heat shock factor). Acute heavy-metal exposures to 0.5 or $1.0\;{\mu}M$ of cadmium (Cd), copper (Cu), manganese (Mn), nickel (Ni) or zinc (Zn) showed that MT transcription was significantly stimulated by Cd (9.6-fold relative to non-exposed control) and Cu (10.4-fold), only moderately by Mn (2.4-fold), but hardly by Ni and Zn. Elevation of water temperature from $25^{\circ}C$ to $31^{\circ}C$ caused a rapid modulation of MT mRNAs toward upregulation to 9.5-fold; however, afterward the elevated mRNA level slightly decreased during further incubation at $31^{\circ}C$ for 6 h. Results from this study suggest that MT-based expression assay could be a useful basis for better understanding the metal- and/or heat-caused stresses in this endangered fish species.

A Study of Joint Reliability According to Various Cu Contents between Electrolytic Ni and Electroless Ni Pad Finish (전해Ni, 무전해 Ni pad에서의 Cu 함량에 따른 접합 신뢰성에 관한 연구)

  • Lee, Hyun Kyu;Chun, Myung Ho;Chu, Yong Chul;Oh, Kum-Sool
    • Journal of the Microelectronics and Packaging Society
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    • v.22 no.3
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    • pp.51-56
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    • 2015
  • It has been used various pad finish materials to enhance the reliability of solder joint and recently Electroless Ni Electroless Pd Immersion Gold (the following : ENEPIG) pad has been used more than others. This study is about reliability according to being used in commercial Electrolytic Ni pad and ENEPIG pad, and was observed behavior of various Cu contents. After reflow, the inter-metallic compound (IMC) between solder and pad is composed of $Cu_6Sn_5$ (Ni substituted) by using EDS, and in case of ENEPIG, between IMC and Ni layer was observed the dark layer ($Ni_3P$ layer). Additional, it could be controlled the thickness of dark layer according to Cu contents. Investigated the different fracture mode between electrolytic Ni and ENEPIG pad after drop shock test, in case of soft Ni, accelerated stress propagated along the interface between $1^{st}$ IMC and $2^{nd}$ IMC, and in case of ENEPIG pad, accelerated stress propagated along the weaken surface such as dark layer. The unstable interface exists through IMC, pad material and solder bulk by the lattice mismatch, so that the thermal and physical stress due to the continuous exterior impact is transferred to the IMC interface. Therefore, it is strongly requested to control solder morphology, IMC shape and thickness to improve the solder reliability.

Effects of Dietary Supplementation of Copper Soy Proteinate (Cu-SP) and Herbal Mixture (HBM) on the Performance, Blood Parameter and Immune Response in Laying Hens (Copper Soy Proteinate(Cu-SP)와 Herbal Mixture(HBM)의 급여가 산란계의 생산성, 혈액성상 및 면역체계에 미치는 영향)

  • Kim, Chan Ho;Kang, Hwan Ku;Bang, Han Tae;Kim, Ji Hyuk;Hwangbo, Jong;Choi, Hee Cheol;Paik, In Kee;Moon, Hong Kil
    • Korean Journal of Poultry Science
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    • v.41 no.4
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    • pp.323-329
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    • 2014
  • The objective of this experiment was to investigate the effect of dietary supplementation of copper-soy proteinate (Cu-SP) and herbal mixture (HBM) on growth performance, blood parameter, and immune response in laying hens. A total 800 Hy-Line Brown laying hens (60 weeks old) were randomly allotted to 1 of 4 dietary treatments : (1) Control : control diet, (2) Cu-SP : control diet + 100 mg/kg Cu-soy proteinate, (3) HBM : control diet + 0.15% herbal mixture, and (4) Cu-SP + HBM : control diet + 100 mg/kg Cu-soy proteinate + 0.15% herbal mixture. Each treatment was replicated 5 times with forty birds units were arranged according to randomized block design. Feeding trial lasted 5 weeks under 16L : 8D lighting regimen. The diet and water were available ad libitum. Result indicated that during feeding trial of the experiment, hen-day egg production was significantly (P<0.05) higher in Cu-SP and HBM treated groups than control. However, feed intake, feed conversion ratio, egg weight, broken and shell less egg production were not significantly influenced by treatments. Eggshell strength was significantly (P<0.05) higher in Cu-SP than control. Eggshell thickness, eggshell color, egg yolk color, Haugh unit were not significantly influenced by treatments. The level of WBC and stress index (heterophil : lymphocyte) were higher in supplemented groups than the control. The concentration of plasma IgG was higher in supplemented groups than the control. The result of this experiment showed that dietary copper-soy proteinate or herbal mixture tended to improve egg production and affect positively on immune response of laying hens.

고온 및 고온고습 가속시험에 의한 CIGS PV 모듈의 열화거동

  • Lee, Dong-Won;Nam, Song-Min;Kim, Yong-Nam
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.08a
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    • pp.421-421
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    • 2012
  • Cu(In,Ga)$Se_2$ (CIGS) 화합물은 직접천이형 반도체로 열적으로 매우 안정하고 에너지밴드갭이 1.04 eV로 이상적인 값에 가깝고, 특히 높은 광흡수계수를 가지기 때문에 박막 태양전지로서 커다란 응용 잠재력을 갖고 있는 광흡수층 재료이다. CIGS 화합물 박막 태양전지의 효율은 연구실에서는 ~20%를 높은 효율을 보고하고 있으며, 모듈급에서도 ~13%의 효율을 보이고 있다. 그러나 CIGS 박막 태양전지를 대면적 또는 양산화에 적용하기 위해서는 20년 이상의 장기적인 수명을 보장할 수 있는 내구성을 갖추어야 한다. 본 연구에서는 CIGS 모듈의 장기적인 신뢰성을 평가하기 위해 CIGS PV 모듈을 대상으로 대표적인 고온 고습 조건인 IEC-61646 규격을 이용하여 $85^{\circ}C$/85% RH에서 1000시간 동안 가속시험이 수행되었고, 고온 환경하에서 모듈의 성능 저하에 미치는 영향을 고찰하기 위해 모듈을 $85^{\circ}C$에서 1000시간 노출시켰다. 두 종류의 가속 스트레스시험 후에 모듈들의 외형적인 노화현상 및 전기적 열화 성능을 분석하였다. 또한 모듈의 효율저하의 원인을 규명하기 위해 모듈 구성 재료 중 충진재료로 사용하는 EVA sheet와 투명전극 AZO를 대상으로 고장분석을 수행하였다. AZO의 미세구조 관찰, 결정상 분석, XPS 분석 및 전기적 분석과 EVA sheet의 FT-IR 분석과 TG-DSC 분석들을 종합하여 CIGS PV 모듈의 성능저하의 원인을 규명하였다.

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