Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Fermented Mulberry Leaves (Morus alba L.) on Oxidative Modification of Antioxidnat Enzymes

항산화 효소의 산화적 변형에 뽕잎 발효물이 미치는 영향

  • 강정훈 (청주대학교 바이오메디컬학과)
  • Received : 2019.09.10
  • Accepted : 2019.09.28
  • Published : 2019.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Muberry (Morus alba L.) leaves fermented with Hericium erinaceum mycelium (MA-HE) were assessed for the protection against oxidative modification of antioxidant enzymes, Cu,Zn-superoxide dismutase(SOD) and ceruloplasmin(CP). MA-HE were shown to significantly inhibited oxidative modifications and inactivations of Cu,Zn-SOD and CP induced by peroxyl radical. Antioxidant activity of MA-HE evaluated using peroxyl radical scavenging assays. MA-HE showed 44.03% of peroxyl radical scavenging activity at $100{\mu}g/mL$. Thus, MA-HE protect the antioxidant enzymes from oxidative damage by the scavenging peroxyl radicals. The results suggested that MA-HE was effectively removed reactive oxygen species in cells, thereby protecting cytotoxicity caused by oxidative stress.

뽕잎 (Morus alba L.)을 노루궁뎅이 버섯균사체(Hericium erinaceum)로 발효시켜 열수 추출한 발효물(MA-HE)이 항산화 효소인 Cu,Zn-superoxide dismutase (SOD)와 ceruloplasmin (CP)의 산화적 변형에 미치는 영향을 관찰하였다. 세포질에서 중요한 항산화 활성을 나타내는 Cu,Zn-SOD와 CP는 모두 구리이온을 포함하는 단백질이다. MA-HE은 peroxyl radical에 의해 변형되는 Cu,Zn-SOD와 CP를 모두 보호하였다. MA-HE은 peroxyl radical에 의한 Cu,Zn-SOD의 단백질 fragmentation과 효소의 inactivation을 모두 효과적으로 억제하였다. 또한, MA-HE는 CP의 fragmentation과 inactivation도 효과적으로 억제하였다. MA-HE가 peroxyl radical을 소거할 수 있는 기능을 가지고 있는 지를 확인하기 위해 thiobarbituric acid를 이용한 활성 측정법으로 관찰한 결과, MA-HE는 농도가 증가함에 따라 비례적으로 peroxyl radical 소거 활성이 높아지는 것으로 관찰되었다. MA-HE는 $100{\mu}g/mL$에서 44.03% 소거활성을 나타냈다. 따라서, MA-HE는 peroxyl radical을 효과적으로 소거함으로서 세포 내의 항산화 효소를 산화적 손상으로부터 보호할 것으로 생각된다. 이와 같은 결과는 MA-HE가 세포 내에서 발생하는 활성산소종을 효과적으로 소거하여 산화적 스트레스에 의해 야기되는 세포 독성에 대한 보호 작용을 할 것으로 생각된다.

Keywords

References

  1. C. H. Jeong, O. S. Joo, K. H. Shim, "Chemical components and physiological activities of young mulberry stem", J. Korean Food Preserv., Vol.9, No.2 pp. 228-233, (2002).
  2. H. B. Kim, C. K. Kang, G. B. Sung, S. W. Kang, J. R. Lee, "Antioxidative capacity of mulberry leaf and its tea", J. Seri. Ento. Sci., Vol.49, No.1 pp. 18-22, (2007).
  3. D. C. Kim, M. J. In, H. J. Chae, "Preparation of mulberry leaves tea and its quality characteristics", J. Appl. Biol. Chem., Vol.53, No.1 pp. 56-59, (2010). https://doi.org/10.3839/jabc.2010.010
  4. J. Y. Chae, J. Y., Lee, I. S. Hoang, D. Whangbo, P. W. Choi, W. C. Lee, J. W. Kim, S. Y. Kim, S. W. Choi, S. J. Rhee, "Analysis of functional components of leaves of different mulberry cultivars", J. Korean Soc. Food Sci. Nutr., Vol.32, No.1 pp. 15-21, (2003). https://doi.org/10.3746/jkfn.2003.32.1.015
  5. M. Y. Park, K. S. Lee, M. K Sung, "Effects of dietary mulberry, Korean red ginseng, and banaba on glucose homeostatis in relation to PPAR-${\alpha}$, PPAR-${\gamma}$, and LPL mRNA expressions", Life Sci., Vol.77, No.26 pp. 3344-3354, (2005). https://doi.org/10.1016/j.lfs.2005.05.043
  6. J. R. Lee, Y. J. Hah, J. W. Lee, Y. M. Song, S. K. Jin, I. S. Kim, K. H. Hah, S. J. Kwak, "Physico-chemical and sensory properties of emulsified sausages containing mulberry and persimmon leaf powder", Korean J. Food Sci. Ani. Resour., Vol.22, No.4 pp. 330-336, (2002).
  7. C. Hansawasdi, J. Kawabata, "${\alpha}$-Glucosidase inhibitory effect of mulberry (Morus alba) leaves on Caco-2", Fitoterapia, Vol.77, No.7-8 pp. 568-573, (2006). https://doi.org/10.1016/j.fitote.2006.09.003
  8. M. G. Kang, W. S. Jo, W. H. Kim, S. Y. Choi, S. D. Park, "The difference of occurring pattern of Hericium erinaceus by pinheading induction methods", J. Mushrooms, Vol.13, No.1 pp. 11-15, (2015). https://doi.org/10.14480/JM.2015.13.1.11
  9. T. Mizuno, T. Wasa, H Ito, C. Suzuki, N. Ukai, "Antitumor-active polysaccharides isolated from the fruiting body of Hericium erinaceum, an edible and medicinal mushrooms called yamabushiotake or houtou", Biosci. Biotechnol. Biochem., Vol.56, No.2 pp. 347-348, (1992). https://doi.org/10.1271/bbb.56.347
  10. T. Mizuno,"Yamabushitake, Hericium rinaceum: Bioactive substances and medicinal utilization", Food Reviews International, Vol.11 pp. 173-178, (1995). https://doi.org/10.1080/87559129509541027
  11. H. Kawagishi, M. Ando, K., H. Shinb, H. Sakamoto, S. Yoshida, F. Ojima, Y. Ishiguro, N. Ukai, S. Furukawa, "Chromans, hericenones F, G and H from the mushroom Hericium erinaceum", Phytochemistry, Vol.32, No. pp. 175-178, (1992). https://doi.org/10.1016/0031-9422(92)80127-Z
  12. K. Mori, S. Inatomi, K. Ouchi, Y. Azumi, T. Tuchida, "Improving effects of the mushroom Yamabushiotake (Hericium erinaceus) on mild cognitive impairment: a double-blind placebo-controlled clinical trial", Phytoher. Res., Vol.23, No.3 pp. 367-372, (2009). https://doi.org/10.1002/ptr.2634
  13. H. Kawagishi, A. Shimada, S. Hosokawa, H. Mori, H. Sakamoto, Y. Ishiguro, S. Sakemi, J. Bordner, N. Kojima, S. Furukawa, Erinacines E, F and G, "Stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum", Tetrahedron Letters, Vol.37, No.41 pp. 7399-7402, (1996). https://doi.org/10.1016/0040-4039(96)01687-5
  14. J. H. Ha, H. S. Jeog, S. H. Oh, S. S. Jeong, M. H. Jeong, H. S. Jeong, J. H. Jeong, K. W. Yu, H. Y. Lee, "Comparision of Immuno activities of fresh Ginseng cultured Phelinus linteus and Hericium erinaceum mycelium associated whit ultrasonification extraction", Korean J. Medicinal Crop. Sci., Vol.17, No.5 pp. 311-320, (2009).
  15. C. K. Park, H. Kim, Qi. Tu, K. W. Yu, H. S, Jeong, H. Y. Lee, J. H Jeong, "Chemical composition and immunostimulating activity of the fermented Korean Ginseng (Panax ginseng C.A. Meyer) with mushroom mycelium by solid culture", J. Korean Soc. Food Sci. Nutr., Vol.38, No.9 pp. 1145-1152, (2009). https://doi.org/10.3746/jkfn.2009.38.9.1145
  16. Y. Jang, S. H. Shin, B. I. Choi, D. S. Park, J. H. Jeon, Y. M. Cho, J. H. Cho, D. H. Jung, S. Y. Hwang, B. W. Ahn, M. S. Jeong, J. H. Jeong, Y. B. Kim, "Effects of Erinacol(R), Extract of Hericium erinaceum Cultivated with Artemisia iwayomogi, on Alcoholic Hangover and Immune Function", Lab. Anim. Res., Vol.22, No.2 pp. 157-163, (2006).
  17. S. H. Shin, J. Y. Jang, B. I. Choi, JH. H. Jeon, H. J. Ji, S. H. Moon, D. H. Jung, Y. G. Yeo, S. J. Baek, S. J. Hwang, J. H. Chung, Y. B. Kim, "Effects of Erinacol(R), Extract of Hericium erinaceum Cultivated with Artemisia iwayomogi, on Alcoholic Fatty Liver", Lab. Anim. Res., Vol.22, No.2 pp. 165-170, (2006).
  18. B. I. Choi, S. H. Shin, J. Y. Jang, D. S. Park, D. H. Kang, S. S Nahm, Y. G. Yeo, J. H. Cho, S. Y. Hwang, M. S. Jeong, M. S. Jeong, J. H. Jeong, Y. B. Kim, "Single-and repeated-dose toxicities of Erinacol(R), extract of Hericium erinacium cultivated with Artemisia iwayomogi, in rats", Lab. Anim. Res., Vol.22, No.2 pp. 171-179, (2006).
  19. S. C. Kwon, "Biological activities of ethanol extract from Hericium erinaceus mycelium on Angelica keiskei and Angelica keiskei pomace", J. Korean Soc. Food Sci. Nutr., Vol.40, No.12 pp. 1648-1653, (2011). https://doi.org/10.3746/jkfn.2011.40.12.1648
  20. S. S. Kim, I. G. Kyeong, M. L. Lee, D. G. Kim, J. Y. Shin, J. Y. Yang, G. H. Lee, W. S. Eum, J. H. Kang, "Protective effects of Sea Buckthorn (Hippophae rhamnoides L.) leaves fermented with Hericium erinaceum mycelium against oxidative modification of biological macromolecules and cell death", J. Korean Soc. Food Sci. Nutr., Vol.44, No.1 pp. 35-43, (2015). https://doi.org/10.3746/jkfn.2015.44.1.035
  21. P. Amstad, R. Moret, P. Cerutti, "Glutathione peroxidase compensates for the hypersensitivity of Cu,Zn-superoxide dismutase overproducers to oxidant stress", J. Biol. Chem. Vol.269, No.3 pp. 1606-1609, (1994). https://doi.org/10.1016/S0021-9258(17)42068-0
  22. O. Elroy-Stein, Y. Groner, "Impaired neurotransmitter uptake in PC12 cells overexpressing human Cu/Zn-superoxide dismutase--implication for gene dosage effects in Down syndrome", Cell, Vol.52, No.2 pp. 259-267, (1988). https://doi.org/10.1016/0092-8674(88)90515-6
  23. O. Elroy-Stein, Y. Bemstein, Y. Groner, "Overproduction of human Cu/Zn-superoxide dismutase in transfected cells: extenuation of paraquat-mediated cytotoxicity and enhancement of lipid peroxidation", EMBO J., Vol.5, No.3 pp. 615-622, (1986). https://doi.org/10.1002/j.1460-2075.1986.tb04255.x
  24. I. Ceballos-Picot, A. Nichole, P. Briand, G. Grimber, A. Delacourte, A. Defossez, F. Javoy-Aqid, M. Lafon, J. L. Blouin, P. M. Sinet, "Neuronal-specific expression of human copper-zinc superoxide dismutase gene in transgenic mice: animal model of gene dosage effects in Down's syndrome", Brain Res. Vol.552, No.2 pp. 198-214, (1991). https://doi.org/10.1016/0006-8993(91)90084-9
  25. M. B. Yim, P. B. Chock, E. R. Stadman, "Enzyme function of copper, zinc superoxide dismutase as a free radical generator", J. Biol. Chem., Vol.268, No6 pp. 4099-4105, (1993). https://doi.org/10.1016/S0021-9258(18)53585-7
  26. S. M. Kim, J. H. Kang, "Peroxidative activity of human Cu,Zn-superoxide dismutase", Mol. Cells, Vol.7, No1 pp. 120-124, (1997).
  27. N. Takahashi, F. W. Putnam, "Chemical structure and molecular heterogeneity of cerulloplasmin", Tanpakushitsu Kakusan Koso, Vol.29, No.10 pp. 795-814, (1984)
  28. I. M. Goldstein, H. B. Kaplan, H. S. Edelson, G. Weissmann, "Cerulloplasmin. A scavenger of superoxide anion radicals", J. Biol. Chem., Vol.254, No.10 pp. 4040-4045, (1979). https://doi.org/10.1016/S0021-9258(18)50692-X
  29. D. R. Winge, "Normal physiology of copper metabolim", Semin. Liver Dis., Vol.4, No.3 pp. 239-251, (1984). https://doi.org/10.1055/s-2008-1041774
  30. J. Folkman, M. Klagsburn, "Angiogenic factors", Science Vol.235, No.4787 pp. 442-447, (1987). https://doi.org/10.1126/science.2432664
  31. F. J. Walker, J. Fay, "Characterization of an interactiion between protein c and cerulloplasmin", J. Biol. Chem., Vol.265, No.4 pp. 1834-1836, (1990). https://doi.org/10.1016/S0021-9258(19)39903-X
  32. Ryden L. in Copper Proteins and Copper Enzymes Vol III p. 37-100, CRC Press Inc, (1984).
  33. J. M. C. Gutteridge, "Inhibition of the Fenton reaction by the protein caeruloplasmin and other copper complexes. Assessment of ferrodidase and redical scavenging activities", Chem. Biol. Interact., Vol.56, No.1 pp. 113-120, (1985). https://doi.org/10.1016/0009-2797(85)90043-2
  34. B. N. Ames, M. K. Shigenaga, T. M. Hagen, "Oxidants, antioxidants, and the degenerative diseases of aging", Proc. Natl. Acad. Sci. USA., Vol.90, No.17 pp. 7915-7922, (1993). https://doi.org/10.1073/pnas.90.17.7915
  35. B. Halliwell, J. M. Gutteridge, "The importance of free radicals and catalytic metal ions in human diseases", Aspects. Med. Vo.8, No.2 pp. 89-193, (1985). https://doi.org/10.1016/0098-2997(85)90001-9
  36. B. S. Berlett, E. R. Stadtman, "Protein oxidtion in aging, disease, and oxidative stress", J. Biol. Chem., Vol.272, No.33 pp. 20313-20316, (1997). https://doi.org/10.1074/jbc.272.33.20313
  37. P. K. Smith, R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson, D. C. Klenk, "Measurement of protein using bicinchoninic acid", Anal. Biochem., Vol.150, No.1 pp. 76-85, (1985). https://doi.org/10.1016/0003-2697(85)90442-7
  38. J. M. MacCord, I. Fridovich, "Superoxide dismutase. An enzyme function for erythrocuprein (hemocuprein)" J. Biol. Chem., Vol.244, No.22 pp. 6049-6055, (1969). https://doi.org/10.1016/S0021-9258(18)63504-5
  39. F. W. Jr. Sunderman, S. Nomoto, "Measurement of human serum cerulloplasmin by its ${\rho}$-phenylenediamine oxidase activity" Clin. Chem., Vol.16, No.11 pp. 903-910, (1970). https://doi.org/10.1093/clinchem/16.11.903
  40. H. Ohkawa, N. Ohishi, K. Yagi, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Anal. Biochem., Vol.95, No.2 pp. 351-358, (1979). https://doi.org/10.1016/0003-2697(79)90738-3
  41. E. Niki, A. Kawakami, M. Saito, Y. Yamamoto, J. Tsuchiya, Y. Kamiya, "Effect of phytyl side chain of vitamin E on its antioxidant activity", J. Biol. Chem., Vol.260, No.4 pp. 2191-2196, (1985) https://doi.org/10.1016/S0021-9258(18)89536-9
  42. P. L. Fox, C. Mukhopadhyay, E. E.nwald, "Structure, oxidant activity, and cardiovascular mechanism of human cerulloplasmin", Life Sci., Vol.56, No.21 pp. 749-1758, (1995).
  43. C. Mukhopadhyay, E. Ehrenwald, P. L. Fox, "Cerulloplasmin enhances smooth muscle cell- and endothelial cell-mediated low density lipoprotein oxidation by a superoxide-dependent mechanism", J. Biol. Chem., Vol.271, No.25 pp. 14773-14778, (1996). https://doi.org/10.1074/jbc.271.25.14773