Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Alterations of Glutathione Peroxidase Patterns by Stressor Treatment in Rice Seedling Roots

스트레스 물질에 의한 벼 glutathione peroxidase 활성패턴 변화

  • 김윤경 (순천향대학교 생명과학부) ;
  • 이미영 (순천향대학교 생명과학부)
  • Published : 2005.03.31
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Abstract

The effect of various stressors such as reductant ascorbic acid, signalling molecules (salicylic acid and methyl jasmonic acid), heavy metals $(NiCl_2,\;and\;MnSO_4)$ and NaCl on the glutathione peroxidase (GPX) activities and isoenzyme expression patterns were investigated in rice seedling roots. Total GPX activity increased according to the increase of ascorbic acid concentration. Prominent enhancement of GPX1 isozyme due to ascorbic acid contributed to the increase of total GPX activity. GPX showed different reactivity toward salicylic acid and methyl jasmonic acid. GPX activity increased at 0.1 mM salicylic acid, and then decreased thereafter. However, GPX increased gradually in a methyl jasmonic acid concentration-dependent manner, and 3 fold increase of GPX activity was found at 1 mM methyl jasmonic acid. Moreover, GPX1 isozyme increased according to the increase of salicylic acid, while GPX1 isozyme decreased according to the increase of methyl jasmonic acid. When metal ions were treated, GPX activity increased considerably according to the increase of $NiCl_2$ concentration, however, GPX activity increased about 2 fold at 0.5 mM $CuSO_4$ and then decreased. Enhancement of GPX1 isozyme contributed to the increase of total GPX activities in $NiCl_2-treated$ and $MnSO_4-treated$ rice seedlings. Total GPX activity increased 1.7 fold in response to 300 mM NaCl. Especially GPX2 isozyme showed gradual increase according to the increase of NaCl concentration.

동진벼 유묘 뿌리에 환원제인 ascorbic acid, 신호전달물질인 salicylic acid와 methyl jasmonic acid, 중금속인 $NiCl_2$와 $CuSO_4$ 및 NaCl를 다양한 농도로 처리한 후 항산화효소인 glutathione peroxidase(GPX)의 활성과 동위효소의 패턴 변화를 살펴보았다. Ascorbic acid 처리에 의한 GPX의 총활성은 ascorbic acid 농도 증가에 의존적으로 증가하였으며 이러한 GPX활성 증가는 GPX1 동위효소의 현저한 활성증가에 기인하였다. GPX는 신호전달물질인 salicylic acid와 methyl jasmonic acid에 대하여 서로 다른 반응성을 보였다. GPX의 활성은 0.1 mM salicylic acid에 의해 증가하였다가 이후 감소하였다. 이에 비해 GPX는 methyl jasmonic acid의 농도증가에 의존하여 점진적으로 증가하여 1 mM methyl jasmonic acid에 의하여 약 3배의 활성증가를 보였다. 뿐만 아니라 GPX1 동위효소는 salicylic acid 농도가 증가할수록 활성이 감소한 반면 methyl jasmonic acid 농도가 증가할수록 현저하게 증가하였다. GPX의 총활성은 $NiCl_2$ 농도 증가에 따라 점진적으로 증가되었으나, $CuSO_4$ 처리군의 경우 GPX의 총비활성도는 0.5 mM $CuSO_4$에 의하여 약 2배 증가한 이후 점차 감소하였다. $NiCl_2$와 $CuSO_4$ 처리에 의한 GPX 활성증가도 주로 GPX1 동위효소의 활성증가에 기인하였다. NaCl 처리에 의한 GPX 총활성은 300 mM NaCl 처리군에서 약 1.7배 증가되었다가 이후 감소하였다. 특이하게도 NaCl 농도가 증가함에 따라 GPX2 동위효소 활성이 점차 증가하였다.

Keywords

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