• Natural Product Sciences
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• v.9 no.4
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• pp.235-240
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• 2003

Tectorigenin has an apoptosis-inducing ability and immunosuppressive activity. We investigated the effect of tectorigenin on Phase I and II enzyme activities to elucidate the pharmacological action of the immunosuppressive tectorigenin in the diabetic rat. This compound was obtained from the hydrolysis of tetoridin isolated from the flower of Pueraria thumbergiana (Leguminosae). This crude drug (Puerariae Flos) has been used as a therapeutic for diabetes mellitus in traditional Korean medicine. Tecotrigenin inhibited the formation of malondialdehyde (MDA) and hydroxy radicals in serum and liver but promoted superoxide dismutase (SOD) activity. Low MDA contents and low xanthine oxidase and aldehyde oxidase activities were observed in the tectorigenin-treated rats, suggesting that such Phase I enzyme activities are the major source of lipid peroxidation. However, tectorigenin increased Phase II enzyme activities such as SOD, glutathione peroxidase and catalase, suggesting the activation of free radical-scavenging enzymes. The activities of tectorigenin were comparable to those of glibenclamide, which was employed as a positive control. These results suggest that tectorigenin may share some biological properties with glibenclamide in insulin-dependent-diabetes mellitus (IDDM).

• Proceedings of the Ginseng society Conference
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• 1993.09a
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• pp.127-131
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• 1993

The effects of total ginseng saponin. extracts of Panax ginseng C.A. Meyer, on the differentiation of F9 teratocarcinoma stem cells were studied. F9 stem cells cultured in the presence of ginseng saponin together with dibutyric cAMP became parietal endoderm - like cells. Moreover, the expressions of differentiation marker genes. laminin. type IV collagen. and retinoic acid $receptor-{\beta}(RAR{\beta})$ were increased after treatment of ginseng saponin. Among various ginsenosides purified from crude ginseng saponin, $Rh_1\;and\;Rh_2$ caused the differentiation of F9 cells most effectively. Since ginsenosides and steroid hormone show resemblance in chemical structure. we studied the possibility of the involvement of a steroid receptor in the differentiation process induced by ginsenosides. According to Southwestern blot analysis, a 94 kDa protein regarding as a steroid receptor was detected in F9 cells cultured in the medium containing ginseng saponin. Based on these data, we suggest that ginseng saponin, especially ginsenosides $Rh_1\;and\;Rh_2$ cause the differentiation of F9 cells and the effects of ginsenosides might be exerted via binding with a steroid receptor or its analogous nuclear receptor.

• Korean Journal of Pharmacognosy
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• v.12 no.4
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• pp.195-199
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• 1981

'Kangsim-San' is an added and subtracted prescription of 'Cheongsim-Yeonza-Tang' recorded in 'Dongeuy-Soose-Boweon' from which it has become one of the favorate prescriptions at the Oriental Medical Hospital, Kyung-Hee University. It is described in the book that the 'Cheongsim-Yeonza-Tang' can be effective to symptoms relating to fatigue, nocturnal emission, abdominal pain, tongue deviation and palsy, etc. However the 'Kangsim-San' has been used for neurotic syndrome, pulpitation, insomnia, constipation, dry-mouth, auorexia and arrythmias and so on, added to the above-mentioned symptoms for the 'Cheongsim-Yeonza-Tang' at the hospital. Nevertheless, the pharmacological research of fundamental basis is not completed so far, and we have attempted experiment on various animals to study the pharmacological effects of the medicine. The result was proved as follows; The prescription had a considerable effects on the sedation of central nervous system, antipyretic and analgesic action, and vasodilative action.

• Ocean and Polar Research
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• v.29 no.2
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• pp.187-191
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• 2007

In this study, antioxidant activities of acetone/dichloromethane and methanol extracts of Arctic seaweeds were investigated. The antioxidant properties of both extracts of arctic seaweed were evaluated using two different peroxynitrite tests, including scavenging power on authentic peroxynitrite and inhibitory activities on peroxynitrite generation from 3-morpholinosydnonimine (SIN-1) producing superoxide anion and nitric oxide simultaneously in vitro. At concentration of $10\;{\mu}g/ml$, the acetone/dichloromethane and methanol extracts of Odonthalia dentata exhibited 54.6 and 64.2% inhibition against peroxynitrite generation from SIN-1 while they exhibited 24.3 and 23.0% scavenging activities on authentic peroxynitrite, respectively. On the other hand, the acetone/dichloromethane extract of Polysiphonia stricta exhibited 61.8% inhibition only against the induced peroxynitrite from SIN-1. Furthermore, the crude extracts of Odonthalia dentata and Polysiphonia stricta were fractionated into n-hexane, 85% aq. MeOH, n-BuOH, and $H_2O$ fractions, successively, and only 85% aq. MeOH fraction exhibited the best inhibition.

• Korean Journal of Pharmacognosy
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• v.32 no.2 s.125
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• pp.93-97
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• 2001

Aurantii Fructus has been used in traditional medicine as a digestant, expectorant, and in the treatment of diarrhea, anal prolapse. For the quality control of this crude drug, hesperidin was isolated from the ethylacetate fraction of Citrus aurantium (Rutaceae) and identified by the spectroscopic evidences. A quantitative analysis of hesperidin using HPLC method exhibited that the average contents of hesperidin were $2.13{\pm}1.16%$ in 57 samples collected throughout the various regions of Korea.

• Korean Journal of Pharmacognosy
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• v.32 no.2 s.125
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• pp.98-102
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• 2001

Evodiae Fructus has been used in traditional medicine for the treatment of gastro- intestinal disorders, headache and migraine, and as a cardiotonic and analgesic. For the quality control of this crude drug, evodiamine was isolated from the methylene chloride extract of Evodia officinalis (Rutaceae) and identified by the spectroscopic evidences. A quantitative analysis of evodiamine using HPLC method exhibited that the average contents of evodiamine were $0.84{\pm}0.60%$ in 53 samples collected throughout the various regions of Korea.

• Korean Journal of Pharmacognosy
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• v.40 no.1
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• pp.18-24
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• 2009

GCSB-5 preparation is a purified extract from a mixture six herbal medicines (Acanthopanacis Cortex, Achyranthis Radix, Saposhnikoviae Radix, Cibotii Rhizoma, Glycine Semen Nigra, Eucommiae Cortex) that have been widely used in traditional medicine to treat various bone disorders. This study was carried out to obtain the HPLC analysis method that can be used to establish quantitative analysis of Glycine Semen Nigra and Eucommiae Cortex for standardization of GCSB-5 preparation. HPLC analysis methods for the simultaneous determination of genistin (Glycine Semen Nigra) and geniposide (Eucommiae Cortex) were established for the quality control of herbal medicinal raw material and preparation. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline. As the result of quantitative analysis, the contents of genistin and geniposide in the raw material of GCSB-5 preparation were 0.0426-0.0427 mg/g and 0.431-0.432 mg/g. And GCSB-5 preparation contained genistin of 0.0202-0.0203 mg/capsule and geniposide of 0.211-0.212 mg/capsule, respectively.

• Proceedings of the Plant Resources Society of Korea Conference
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• 1999.10a
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• pp.58-64
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• 1999

As indicated by Chinese letters "사혁" (sha-ji) is the ripe fruit of Seabuckthron (Hippophae rhamnoides Linneus) Family Flaeagnaceae is one of ancient natural medicine used by the Tibetan and Mongolian nationallites with ancient methods to treat desease, It is an effectiv Traditional Tibetan medicine. Bud only now are they beginning to be understood in the world of modern medicine. Hippophae was written in the book of Traditional Tibetan Medicine - "Somalaza" (8th century), "Sibuyidian" (1840) and "Jingzhubencao" (Qing Dynasty). The "Sibuyidien" was trnslated in to the Russian and published at 1903 in the Russian. Then the Russian bigan to study on Hippophae and they have achieved great successes. The Chinese scientists began to study on the chemical constituent and medical action with modern technique at 1952. With more than 40 years painstaking eport they have conceived and used a wide variety of experimental screening systems, achieved successes. Finally the)1 with one's own tests has keen verified that the crude drug being various kind of bioactive substance could Inhibits tumor, increase immunity, wipe out free radical, prevent disease of cardiovascular system, have anti-radiation, anti-inflammeutory effects. At 1977 the Hippophae rhamnoides was written in Chinase Pharmacopoeia.e rhamnoides was written in Chinase Pharmacopoeia.

• Journal of Ginseng Research
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• v.26 no.4
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• pp.187-190
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• 2002

Crude synaptic membrane fractions from the frontal cortex, striatum, brain stem and whole brain of rat were prepared to assay the effects of ginseng total saponin (GTS) on [$^3$H]DAGO bindings of the opioid $\mu$-receptors. Scatchard plots analysis binding data demonstrated that GTS (0.1 mg/ml) decreased the affinity of specific [$^3$H]DAGO bindings without changes in B$\_$max/ in the frontal cortex and striatum. On the other hand, GTS did not affect the [$^3$H]DAGO bindings iii the brain stem and whole brain. These results suggest that the regulation of [$^3$H]DAGO bindings by GTS may play roles in the change of the pharmacological responses of $\mu$-opioids.

• YAKHAK HOEJI
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• v.36 no.2
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• pp.140-149
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• 1992

Anti-allergic action of each water extracts of some crude drugs was investigated in mice and rats. The activity of hyaluronidase which was used in the screening test of anti-allergic action was inhibited significantly by Amomi Semen, Asiasari Radix, Cimicifugae Rhizoma, Cinnamomi Ramulus, Glycyrrhizae Radix and Scutellariae Radix. The 48-hour homologous passive cutaneous anaphylaxis(48-hr PCA) in mouse ear was inhibited significantly by intraperitoneal(i.p.) injection of Amomi Semen, Cimicifugae Rhizoma, and ketotifen, a comparative drug of an anti-allergic action. The increase of vascular permeability induced by histamine or serotonin was inhibited significantly by i.p. injection of Amomi Semen, Cimicifugae Rhizoma, Cinnamomi Ramulus and ketotifen. In rat dorsal skin, the increase of vascular permeability which was induced by histamine, serotonin or compound 48/80 was inhibited significantly by i.p. injection of Amomi Semen, Asiasari Radix, Cimicifugae Rhizoma, Scutellariae Radix and ketotifen. Armeniacae Semen and Liriopis Tuber which had not inhibited hyaluronidase activity did not inhibit 48-hr PCA and the increase of histamine, serotonin or compound 48/80-induced vascular permeability in mice and rats. These results suggest that each water extract of Amomi Semen and Cimicifugae Rhizoma has anti-allergic action.