Browse > Article
http://dx.doi.org/10.5142/JGR.2002.26.4.187

Effects of Ginseng Total Saponin on [3H]DAGO Bindings of Opioid μ-Receptors  

Oh, Ki-Wan (Department of Pharmacology, College of Pharmacy, Chungbuk National University)
Lim, Hwa-Kyung (Korea Food and Drug Administration)
Park, Cheol-Beum (Department of Pharmacology, College of Pharmacy, Chungbuk National University)
Shin, Im-Chul (Department of Pharmacology, College of Pharmacy, Chungbuk National University)
Hong, Jin-Tae (Department of Pharmacology, College of Pharmacy, Chungbuk National University)
Publication Information
Journal of Ginseng Research / v.26, no.4, 2002 , pp. 187-190 More about this Journal
Abstract
Crude synaptic membrane fractions from the frontal cortex, striatum, brain stem and whole brain of rat were prepared to assay the effects of ginseng total saponin (GTS) on [$^3$H]DAGO bindings of the opioid $\mu$-receptors. Scatchard plots analysis binding data demonstrated that GTS (0.1 mg/ml) decreased the affinity of specific [$^3$H]DAGO bindings without changes in B$\_$max/ in the frontal cortex and striatum. On the other hand, GTS did not affect the [$^3$H]DAGO bindings iii the brain stem and whole brain. These results suggest that the regulation of [$^3$H]DAGO bindings by GTS may play roles in the change of the pharmacological responses of $\mu$-opioids.
Keywords
Ginseng total saponin (GTS); [$^3$H]DAGO; ${\mu}$-opioid; synaptic membrane; receptor bindings;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Takagi, K., Saito, H. and Nabata, H. : Pharmacological studies of panax ginseng root: estimation of pharmacological actions of panax ginseng root. Jpn. J. Pharmac. 22, 245-259 (1972)   DOI
2 Ito, Y. and Kuriyama, K. : Some properties of solubilized GABA receptor. Brain Res. 236, 351-363 (1982)   DOI   ScienceOn
3 Saito, H., Tsuchiya, M., Nata, S. and Takagi, K. : Effects of panax ginseng root on the acquisition of second discrimination behavior in rats. Jpn. J. Pharmacol. 27, 509-616 (1977)   DOI
4 Saito, H., Tsuchiya, M., Nata, S. and Takagi, K. : Effects of panax ginseng root on conditional avoidance response in rat. Jpn. J. Pharmacol. 29, 310-325 (1977)   DOI
5 Oh, J. S., Park, C. W. and Moon, D. Y. : Effects of panax ginseng on the central nervous system. Kor. J. Pharmacol. 5, 23-28 (1969)
6 Lowery, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. : Protein measurement with the Folin phenol reagent. J. BioI. Chem. 193, 265-275 (1951)
7 Kim, H. S., Jang, C. G., Oh, K. W., Oh, S., Rheu, H. M., Rhee, G. S., Seong, Y. H. and Park, W. K. : Effects of ginseng total saponin on morphine-induced hyperactivity and conditioned place preference in mice, J. Ethnopharamcol. 60, 33-42 (1998)   DOI   ScienceOn
8 Kim, H. S., Jang, C. G., Seong, Y. H., Nam, K. Y. and Oh, K. W.: Blockade by ginseng total saponin pf the development of cocaine-induced reverse tolerance and dopamine receptor supersensitivity in mice. Pharmacol. Biochem. Behav. 50, 23-27 (1995)   DOI   ScienceOn
9 Kim, H. S., Jang, C. G., Rheu, H. M., Cho, D.H. and Oh, K. W. : Blockade by ginseng total saponin pf the development of methmaphetamine reverse tolerance and dopamine receptor supersensitivity in mice. Planta Medica 61, 22-25 (1995)   DOI   ScienceOn
10 Petkov, V. W. : Pharmacological investigation of the drug Panax ginseng C. A. Meyer. Arzneim. Forsch. (Drug Res.). 9, 305-311 (1939)
11 Kimura, T., Saunders, P. A., Kim, H. S., Rheu, H. M., Oh, K. W. and Ho, I. K. : Interactions of ginsenosides with ligand-bindings of $GABA_A$ and $GABA_B$ receptors. Gen. Pharmacol. 25(1), 193-199 (1994)   DOI   ScienceOn
12 Tsang, D., Yeung, H. W., Tso, W. W., Peck, H. and Lay, L. P. : Effect of saponins isolated from ginseng on the uptake of neurotransmitter in rat brain synaptosomes. Neurosciences Letter (supp.) 12, S20 (1983)
13 Kim, Y. C., Lee, J. H., Kim, M. S. and Lee, N. G. : Effect of the saponin fraction of panax ginseng on catecholamines in mouse brain. Arch. Pharm. Res. 8, 45-39 (1985)
14 Kim, H. S., Jang, C. G. and Lee, M. K. : Antinarcotic effects of standardized ginseng G115 on morphine, Planta Medica 56, 158-163 (1990)   DOI   ScienceOn
15 Ramarao, P. and Bhargava, H. N. : Antagonism of the acute pharmacological actions of morphine by Panax ginseng extract, Gen. Pharmacol. 21, 877-880 (1990)   DOI   ScienceOn
16 Kim, H. S., Oh, K. W., Oh, S., Rheu, H. M. and Seong, Y. H. : Antagonism of morphine analgesia by the pretreatment sites with ginseng total saponin, Kor. J. Ginseng Sci. 15(1), 1-6 (1991)
17 Glowinski, J. and Iversen, L. L. : Regional studies of cate-cholamines in the brian: The disposition of [$^3H$]rorepinephrine, [$H^3$]dopanine and [$^3H$]DOPA in various regions of the brain. J. Neurochem. 13, 655-669 (1966).   DOI
18 Kim, H. S., Jin-Goo Kang, and Oh, K. W. : Inhibition by ginseng total saponin of the development of morphine reverse tolerance and dopamine receptor supersensitivity in mice, Gen. Pharmacol. 26(5), 1071-1076 (1995)   DOI   ScienceOn
19 Kim, H. S., Jang, C. G., Oh, K. W., Seong, Y. H., Rheu, H. M., Cho, D.H. and Kang, S. Y. : Effects of ginseng total saponin on cocaine-induced hyperactivity and conditional place preference in mice Pharmacol. Biochem. Behav. 53, 185-190 (1996)   DOI   ScienceOn
20 Namba, T, Yoshizaki, T., Tominori, K., Kobashi, K., Mitsui, K. and Hasse, J. : Fundamental studies on the evaluation of the crude drugs (1), Planta Med. 32, 588-394 (1974)
21 Segal, D. S. and Kuczenski, R. : Tyrosine hydroxylase activity; regional and subcellular distribution in brain. Brain Res. 68, 261-266 (1974)   DOI   ScienceOn
22 Zukin, S. R., Young, A. B. and Snyder, S. H. : Gamma aminobutyric acid binding to receptor sites in the rat central nervous system. Proc. Natn. Acad. Sci. USA. 71, 4802-4807 (1974)   DOI   ScienceOn