• Korean Journal of Microbiology
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• v.51 no.2
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• pp.115-125
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• 2015

A monoclonal antibody AP64 IgM binds to human acute nonlymphocytic leukemia (ANLL) cell line HL60 and also cross-reacts with the homologous antigen in a rat ANLL cell. This antibody mediated by complement, has leukemia a suppression effect. In this study, we generated a recombinant single-chain variable domain fragment (ScFv) which were derived from $V_H$ and $V_L$ cDNA of AP64 IgM-secreting hybridoma by RT-PCR. The two variable regions were joined with a single 15 amino acid linker $(G_4S)_3$. This recombinant ScFv was expressed as a single polypeptide chain from Escherichia coli BMH 71-18. The recombinant ScFv was purified by applying the periplasmic extract to $Ni^+$-NTA-agarose affinity column and detected with westernblot. The purified recombinant ScFv recognized a surface antigen (about 30 kDa) of HL60 cell line which is the same antigen detected by parental AP64 IgM. But the affinity of ScFv for a surface antigen of HL60 was lower than that of the parental AP64 IgM, which needs to be further improved. Overall, the recombinant ScFv specific to HL60 might be a useful bioreagent for either diagnostic or therapeutic purposes.

• Asian Journal of Innovation and Policy
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• v.12 no.1
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• pp.75-100
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• 2023

Over the past few decades, globalization has been shifting economic power upward to transnational actors on the one hand, and downward to subnational or regional spaces on the other. This phenomenon has resulted in the centrality of territorially delimited subnational regions acting as critical loci of economic governance within a complex and globally distributed value chain of trade and service flows. Within this broader context of industrial restructuring are economic regions that span national borders in their collective assets. The paper focuses on investigating the economic competitiveness and productivity of cross-border (or binational) economic regions. Using the conceptual framework of economic clusters, an econometric model that measures proxies of geographic proximity of firms in the life sciences cluster, and a new binational economic model, the paper examines the key characteristics, potentials and constraints of economic competitiveness and productivity in a cross-border region comprising counties in Western New York and regional municipalities in Southern Ontario. The findings demonstrate the direct and indirect benefits of closer cross-border economic cooperation. The paper then concludes with some policy observations about leveraging cross-border economic clusters for strategic industrial cooperation.

• Bulletin of the Korean Chemical Society
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• v.16 no.4
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• pp.360-363
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• 1995

The photopolymerization of methyl methacrylate(MMA) with phenylsilane wasperformed. The molecular weights of the poly(MMA) containing SiH moieties were increased with augment of molar ratio of PhSiH3 over MMA by cross-linking via hydrosilation at the expense of isolated yield. Phenylsilane apparently influenced on the photopolymerization as a chain transfer agent and solvent.

• Journal of the Korean Operations Research and Management Science Society
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• v.40 no.1
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• pp.57-73
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• 2015

While many previous studies investigated the effect of operations performance on financial performance, most studies considered only a few performance indicators and ignored the characteristics of industries. Therefore, this study intends to analyze the effect of operations performance on financial performance, by selecting a rather comprehensive operations performance indicators from firms' financial data. In doing so, we used operating efficiency and supply chain performance indicators for operations performance and a firm's profitability and future value indicators for financial performance. For the analysis, we collected 544 firms' operations and financial performance data belonging to eight key industries from the 'Forbes Global 2000'. We first analysed the differences in operations and financial performance among high, medium and low supply chain performance groups based on the quantitative criteria of Gartner's 'Supply Chain Top 25' ranking procedure. Then we analysed the effect of operations performance indicators on financial performance for both entire industry and individual industries, using multiple regression. Based on the results, we provided practical insights into key operations performance indicators to focus on and manage in order to improve financial performance.

• Membrane Journal
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• v.28 no.3
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• pp.205-213
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• 2018

In this study, cross-linked membrane were successfully prepared by using brominated PPO (Br-PPO) as the main polymer chain. Chitosan and quaternary ammonium modified chitosan (QA-chitosan) was used as the cross linking agents. The cross linked membranes were post-functionalized by using trimethylamine solution. The degree of cross linking was also controlled by varying the ratio of cross linking agent. The applicability of the cross-linked membrane (A-PPO + chitosan, A-PPO + QA-chitosan) as ion exchange membranes was verified through various characterization techniques. The cross-linked membrane using QA-chitosan as cross linking agent was found to be better in performance than the membrane using pristine chitosan cross linking agent. As the percentage of QA-chitosan increased, the ion exchange capacity from 1.18 meq/g to 1.53 meq/g and water uptake from 21.6% to 42.2% was improved.

• International Journal of Internet, Broadcasting and Communication
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• v.6 no.1
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• pp.9-12
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• 2014

We present a mathematical method for calculation of transmission zero locations, determining a filtering characteristics of two-port systems. By adjusting element values based on the zero locations, the frequency-selectivity is characterized. The characteristic polynomial of ladder networks in externally-loaded feed-forward systems is considered by adopting chain matrices for subsystems. This method can be extended to other types of lumped systems with cross-coupled sections. We find out the zeros by solving characteristics polynomials of closed-form expressions in terms of Laplace impedances of elements. The pairs of complex zeros are shown to be solely from the cross-coupled portion of the system.

• Asian Journal of Innovation and Policy
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• v.11 no.2
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• pp.238-257
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• 2022

As the global epidemic continues to worsen, the pressure on the supply chain is also increasing. In this context, Internet shopping has ushered in new development opportunities. Under the influence of globalization, the development of cross-border e-commerce (CB-EC) is particularly remarkable. As China is a big manufacturing country, the growth of CB-EC is a significant opportunity for Chinese goods to enter the international market. Therefore, this paper comprehensively analyzes the current situation of the integrated development of China's manufacturing industry and CB-EC from four aspects: the operation mode, development mode, policy environment, and development prospect of CB-EC in China's manufacturing sector. This paper constructs an evaluation system including 19 secondary indicators to rank the CB-EC development environment of 30 provinces in China that have established comprehensive pilot zones of CB-EC.

• International Journal of Precision Engineering and Manufacturing
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• v.9 no.3
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• pp.55-58
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• 2008

Hydroforming is a forming technology in which a steel tube is set in a die and formed to fit a specified shape by applying hydraulic pressure from inside the tube while also applying force in the tube axial direction (axial feed). In present study, the entire design process chain for an automotive cross-member was simulated and developed using hydroforming technology on high-strength steel. The part design stage required a feasibility study. The process was designed using computer-aided design techniques to confirm the actual hydroformability of the part in detail. The possibility of using hydroformable cross-member parts was examined using cross-sectional analyses, which were essential to ensure the formability of the tube material for each forming step, including pre-bending and hydroforming. The die design stage included all the components of a prototyping tool. Press interference was investigated in terms of geometry and thinning.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.13 no.4
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• pp.99-107
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• 2003

In this paper we propose an international cross certification model using cross certificate. We propose a new model by analyzing and solving current problems of the National PKI. We recommend a certificate profile, design a directory schema, and propose a method to access PSE(personal security environment) using PKCS＃11, which gives the expansibility and convenience. Finally, we propose a certificate path verification method using RFC 3280 and show how to get the certificate chain by using the trust anchor. This model is recommended to the detailed level of specification for the interoperability of each country's PKI.

• Journal of Intelligence and Information Systems
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• v.16 no.2
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• pp.1-17
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• 2010

Association rules among product items by association analysis suggest sales effect among products. These are useful for marketing strategies such as cross-selling and product display etc. However, if we evaluate more practical product values reflecting cross-selling effects, they will be also more useful for the decisions of companies such as product item selection for product assortment and profit maximization etc. This study proposes product value evaluation models with the concept of effective value based on single-item association chain and itemset association chain. In addition to that, we performed experiments with transaction data related to clothing of an online shopping mall in Korea to show the performances of our models. In result, we confirmed that some items increased in effective values compared with their pure values while the others decreased in effective values.