• Animal Bioscience
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• 제35권9호
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• pp.1444-1453
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• 2022

Objective: Acetate plays an important role in host lipid metabolism. However, the network of acetate-regulated lipid metabolism remains unclear. Previous studies show that mitogen-activated protein kinases (MAPKs) and mechanistic target of rapamycin (mTOR) play a crucial role in lipid metabolism. We hypothesize that acetate could affect MAPKs and/or mTOR signaling and then regulate lipid metabolism. The present study investigated whether any cross talk occurs among MAPKs, mTOR and acetate in regulating lipid metabolism. Methods: The ceramide C6 (an extracellular signaling-regulated kinases 1 and 2 [ERK1/2] activator) and MHY1485 (a mTOR activator) were used to treat rabbit adipose-derived stem cells (ADSCs) with or without acetate, respectively. Results: It indicated that acetate (9 mM) treatment for 48 h decreased the lipid deposition in rabbit ADSCs. Acetate treatment decreased significantly phosphorylated protein levels of ERK1/2 and mTOR but significantly increased mRNA level of hormone-sensitive lipase (HSL). Acetate treatment did not significantly alter the phosphorylated protein level of p38 MAPK and c-Jun aminoterminal kinase (JNK). Activation of ERK1/2 and mTOR by respective addition in media with ceramide C6 and MHY1485 significantly attenuated decreased lipid deposition and increased HSL expression caused by acetate. Conclusion: Our results suggest that ERK1/2 and mTOR signaling pathways are associated with acetate regulated HSL gene expression and lipid deposition.

• Journal of Audiology & Otology
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• 제23권2호
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• pp.63-68
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• 2019

Background and Objectives: To investigate the relationship between hearing impairment and alcohol drinking patterns in South Korean adults. Subjects and Methods: Data collection was performed by Korean National Health and Nutrition Survey from January 1 to December 31, 2012. Data analyses were performed from February 20 to March 3, 2018. Data from 3,860 adults 20 years of age or older without a history of malignancy or chronic otitis media in the Korean National Health and Nutrition Survey 2012 database who participated in the health questionnaires, and who had available results from otologic examinations that included pure tone audiogram, were included. Pure-tone average hearing thresholds were calculated at 500, 1,000, 2,000, and 4,000 Hz. Hearing loss was defined as a pure-tone average >40 dB in one or both ears. The Alcohol Use Disorder Identification Test was used to evaluate drinking statuses of subjects. Data were analyzed using the complex-sample χ²-test of independence and a complex-sample logistic regression analysis. Results: Of the 29,954,319 individuals in the weighted cross-sectional study population, 15,106,040 (50.4%) were men and 14,848,098 (49.6%) were women. A total of 8.1% of men and 7% of women had hearing impairment. The degrees of drinking with appropriate, risky, and hazardous drinking habits were 58.2, 32.1, and 9.7% among men; and 76.4, 12.5, and 11.1% among women, respectively. Among men, the odds ratio of hearing loss increased by 2.506 times when comparing hazardous and appropriate drinking (confidence interval, 1.083 to 5.800, p=0.002). Moderate alcohol consumption (≤2 drinks per day) was not protective for hearing in either group. Conclusions: As hazardous drinking tends to coexist with hearing impairment in men, appropriate prevention and intervention strategies should be emphasized. A longitudinal study to investigate harmful drinking and the mechanism of hearing loss should be performed.

• Safety and Health at Work
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• 제14권1호
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• pp.66-70
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• 2023

Background: Online teaching and learning extend the duration of using gadgets such as mobile phones and tablets. A prolonged usage of these gadgets in a static position can lead to musculoskeletal disorders (MSD). Therefore, this study aims to identify the risk factors related to musculoskeletal symptoms while using gadgets during work from home due to the COVID-19 pandemic. Method: A cross-sectional survey with online-based questionnaires was collected from the University of Indonesia, consisting of lecturers, students, and managerial staff. The minimum number of respondents was 1,080 and was defined by stratified random sampling. Furthermore, the dependent variable was musculoskeletal symptoms, while the independent were age, gender, job position, duration, activity when using gadgets, and how to hold them. Result: Most of the respondents had mobile phones but only 16% had tablets. Furthermore, about 56.7% have used a mobile phone for more than 10 years, while about 89.7% have used a tablet for less than 10 years. A multivariate analysis found factors that were significantly associated with MSD symptoms while using a mobile phone, such as age, gender, web browsing activity, work, or college activities. These activities include doing assignments and holding the phone with two hands with two thumbs actively operating. The factors that were significantly associated with MSD symptoms when using tablets were gender, academic position, social media activity, and placing the tablet on a table with two actively working index fingers. Conclusion: Therefore, from the results of this study it is necessary to have WFH and e-learning policies to reduce MSD symptoms and enhance productivity at work.

• 한국비블리아학회지
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• 제33권1호
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• pp.233-258
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• 2022

본 연구의 목적은 교수매체로써 그림책을 탐색하고자 하는 초등학교 교사들의 접근점에 대한 요구를 파악하고 학교도서관 DLS(Digital Library System)의 접근점 개선방안을 제안하는 것이다. 이를 위해 먼저 국내외 7개 도서관 온라인목록시스템에서 제공하는 접근점을 조사하였다. 그리고 초등학교 교사들을 대상으로 온라인 설문조사를 실시하여 최종적으로 220개의 응답을 분석하였다. 분석 결과, 교사들이 가장 필요로 하는 그림책의 접근점은 주제, 학년/연령, 내용, 교과/단원, 범교과학습주제 등으로 나타났다. 이러한 결과를 바탕으로 본 연구는 필요성이 높게 나타난 접근점을 DLS의 목록레코드에서 제공하고, 통제어휘집을 개발하고, 시스템의 기능이나 인터페이스를 개선하여 그림책에 대한 접근성을 향상시키는 방안을 제안하였다.

• 대한통합의학회지
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• 제11권1호
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• pp.1-12
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• 2023

Purpose : This study examined the relationship between oral health management behavior and subjective health perception by using a tool that measures health-related quality of life named EQ-5D. The findings are expected to provide fundamental data for developing diverse programs, including health- and oral-health-related educational media, to ultimately enhance people's health-related quality of life. Methods : Adults over the age of 20 were extracted from the raw materials of the 8th national health and nutrition examination survey conducted in 2019. Among them, 4034 were finally chosen as the study subjects after checking the response distribution of relevant questions and excluding inadequate samples. Variables included general characteristics, health management behavior, oral health management behavior, subjective health perception, and EQ-5D level. Cross-tabulation test, independent t-test, one-way analysis of variance, and linear regression analysis were conducted using SPSS 25.0. Results : EQ-5D, which measures the level of health-related quality of life, was high when the subjects had a medical check-up experience, no experience of unsatisfactory medical service, and did not get an influenza vaccination (p<.001). The quality of life was higher when the subjects received dental inspections, did not require treatment, and used oral care goods (p<.005). Health-related quality of life was also higher when the subjective health level and subjective oral health level were high. Conclusion : Results indicate that subjective health, oral health perception level, and quality of life together were high when people had a regular check-up, did not neglect treatment, and used oral care goods. Considering the findings of this study, check-up programs that are customized for each age group are necessary. Health-related quality of life could be enhanced through the provision of diverse medical services and active efforts to prevent any medical blind spot.

• 대한청각학회지
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• 제23권2호
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• pp.63-68
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• 2019

Background and Objectives: To investigate the relationship between hearing impairment and alcohol drinking patterns in South Korean adults. Subjects and Methods: Data collection was performed by Korean National Health and Nutrition Survey from January 1 to December 31, 2012. Data analyses were performed from February 20 to March 3, 2018. Data from 3,860 adults 20 years of age or older without a history of malignancy or chronic otitis media in the Korean National Health and Nutrition Survey 2012 database who participated in the health questionnaires, and who had available results from otologic examinations that included pure tone audiogram, were included. Pure-tone average hearing thresholds were calculated at 500, 1,000, 2,000, and 4,000 Hz. Hearing loss was defined as a pure-tone average >40 dB in one or both ears. The Alcohol Use Disorder Identification Test was used to evaluate drinking statuses of subjects. Data were analyzed using the complex-sample χ²-test of independence and a complex-sample logistic regression analysis. Results: Of the 29,954,319 individuals in the weighted cross-sectional study population, 15,106,040 (50.4%) were men and 14,848,098 (49.6%) were women. A total of 8.1% of men and 7% of women had hearing impairment. The degrees of drinking with appropriate, risky, and hazardous drinking habits were 58.2, 32.1, and 9.7% among men; and 76.4, 12.5, and 11.1% among women, respectively. Among men, the odds ratio of hearing loss increased by 2.506 times when comparing hazardous and appropriate drinking (confidence interval, 1.083 to 5.800, p=0.002). Moderate alcohol consumption (≤2 drinks per day) was not protective for hearing in either group. Conclusions: As hazardous drinking tends to coexist with hearing impairment in men, appropriate prevention and intervention strategies should be emphasized. A longitudinal study to investigate harmful drinking and the mechanism of hearing loss should be performed.

• International Journal of Internet, Broadcasting and Communication
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• 제16권1호
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• pp.241-252
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• 2024

We analyze the types of Web3 business model innovation (BMI) of the six major Korean game companies by market size. As a result of the analysis, Nexon is watched as the adapter. It introduces blockchain (BC) layer 2, 'Polygon' to the extended ecosystem such as the creator's secondary creation, item utility, and compensation experience using the existing core intellectual property (IP). KakaoGames and Neowiz are watched as the adventurers. KakaoGames introduces BC layer 2, 'Polygon' and 'Near Protocol' to various experiments using tokenomics models in casual games and massively multiplayer online role-playing games (MMORPGs) using several existing popular IPs. Neowiz also introduces BC layer 2, 'Polygon' and 'Avalanche' to the IntellaX platform using existing game IPs. As the reinventor, Netmable positions as a game publisher that releases third-party games based on multi-chain infrastructure such as Klaytn, BNB Chain, Near Protocol, Aptos Foundation, and introduces BC to new core IPs. Finally, there are Wemade and Com2us as the mavericks. They aim to be the Web3 platform operators that create a BC layer 1 ecosystem and provide services that encompass BC games, GameFi, and non-fungible tokens (NFTs). Here are the implications of the four types of BMI. In terms of infrastructure, Nexon, KakaoGames, and Neowiz try to introduce a part of cross-chain, whereas Netmable tries to move toward a complete multi-chain strategy, and Wemade and Com2us also try to consider multi-chain, even if they have the full BC introduction. In terms of defending against market decline, Nexon and Netmable have a different position. Nexon which has a greater market dominance, only tries to continuously experiment, but Netmable is aggressively focusing on monetizing new products. Attacks on growth aspirations also show two different positions. KakaoGames and Neowiz only try to aggressively explore, while WeMade and Com2us try to set new standards for industrial innovation.

• 한국인터넷방송통신학회논문지
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• 제24권2호
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• pp.161-166
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• 2024

오늘날 청소년은 역사상 가장 디지털 방식으로 상호 연결된 세대로, 전례 없는 수준의 기술적 연결성을 특징으로 하는 시대를 항해하고 있다. 이 연구는 우울의 매개 역할을 중심으로 청소년의 스마트폰 중독이 또래 관계의 질에 미치는 영향을 탐구했다. '한국아동·청소년패널조사 2018'의 2022년 단면 데이터에서 추출한 만 14세 청소년 2,288명의 응답 자료를 분석하여 연구문제를 검증했다. 연구 결과, 청소년의 스마트폰 중독은 우울 증상을 유발하며, 이에 따라 또래 관계의 질이 저하되는 것으로 나타났다. 이 연구의 결과는 디지털 기술과 청소년의 정신적 및 사회적 웰빙의 교차점에 대한 학술적 이해를 넓히는 것에 공헌하며, 보호자, 교사, 상담가, 정책 입안자, 교육 기관의 실무자에게 청소년의 과도한 스마트폰 사용에 대한 모니터링 및 개입 프로그램의 중요성을 시사한다.

• 비교문화연구
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• 제50권
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• pp.31-51
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• 2018

우리시대에 '괴물'은 다시 전성기를 맞고 있다. 좀비, 휴머노이드, 바이러스, 외계인, 연쇄살인범 등을 다루는 수많은 '괴물서사'들이 대중을 사로잡고 있다. 이 논문은 '괴물서사'가 도대체 무엇인지를 질문하면서 괴물서사의 양상들을 점검한다. 괴물이라는 단어는 유일하고 명확한 정의를 빠져나가는 하나의 개념적 원형범주로 사용되며, 그런 의미에서 '은유'로, 아마도 오늘날 가장 익숙한 은유 중 하나로 활용된다. 괴물이 정의를 빠져나가는 이유는 수많은 괴물 형상들을 공통적으로 묶는 요소가 '기이하다, 의심스럽다, 정상이 아니다'라는 추상적 의미일 뿐이기 때문이다. 정상성과 기준을 뛰어넘는 괴물이라는 존재는 몸에서 떨어져 나오고 배설된 것을 뜻하는 비체(abjection)와 통해있다. 혐오와 공포를 생산한다는 점에서 괴물과 비체는 서로를 참조한다. 이 비체적인 것은 인간 범주 바깥의 '다른 것들'(others)을 의미하는 포스트휴먼적인 것과 일맥상통한다. 인간주의가 확립한 경계를 무너뜨리는 포스트휴머니즘이 경계의 무너짐을 나타나는 괴물에 관심을 갖는 것은 이런 점에서 당연하다. 좀비, 휴머노이드, 사이보그, 로봇 등 포스트휴먼적 존재들은 오늘날 가장 대표적인 괴물의 표상들인 것이다. 요컨대 괴물은 인간적, 인간주의적 범주를 뒤흔드는 모든 존재들이고, 괴물서사는 근대적-인간중심적-남성중심적 범주 바깥의 타자에 대한 이야기들이다. 프로이트가 '낯설은 두려움'(Das Unheimliche)이라는 용어로 말하듯이, 괴물이라는 낯선 존재들은 사실 우리에게 원초적으로 낯익고 익숙했던 것들의 회귀이기도 하다. 괴물서사가 결국 인간주의를 뒤흔들고 비판하고 극복하는 서사라는 점에서 볼 때, 오늘날 괴물서사는 '파국서사'에서 가장 전형적으로 나타난다. 파국서사가 인류 현재 문명의 몰락과 그 이후 상황을 서술하는 이야기라고 할 때, 파국서사의 이야기들은 필연적으로 현재의 가치들을 회의하거나 재사유하거나 배제하는 경향을 보인다. 파국서사와 괴물서사는 공히 인간주의적 현재를 비판하면서 그 이후를 사유하며, 이는 오늘날 생태학의 가장 중요한 개념인 인류세와 자본세가 파국서사와 연관된다는 점에서도 중요하다. 파국서사는 기존의 인간주의적 리얼리즘 서사를 넘어서는 곳에서 시작하고, 그런 점에서 그 자체로 괴물서사이며, 현재의 주요한 서사양식과 사유방식을 뒤흔든다는 점에서 괴물적 서사이기도 하다.

• Pediatric Infection and Vaccine
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• 제22권2호
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• pp.106-112
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• 2015

목적: 장기간의 항생제 치료는 중이염 어린이 환자의 중이액으로부터 원인균이 배양되는 것을 방해한다. 본 연구는 배양 음성 중이액으로부터 분자적 진단에 의한 신속한 균 검출 가능성 여부를 확인하고자 하였다. 방법: 폐구균 lytA 유전자를 표적으로 하는 PCR과 LAMP로 민감도와 특이도를 비교 결정하고, 임상중이액에서의 폐구균 검출에 적용하였다. 결과: PCR 기법에 의한 폐구균 검출 최소한계는 약 $10^4$ 집락형성단위(CFU)이고, LAMP의 검출 최소한계는 10 CFU에서 결정되었다. 한편 두 가지 검사법 모두 Haemophilus influenzae 와 Moraxella catarrhalis 에 대해 $10^6$ CFU 이상에서도 DNA를 증폭하지 않았다. 22개의 배양음성 중이액 중에서 12개 검체가 LAMP-양성(54.5%, 12/22)으로 확인되었고, 이들 12개 LAMP-양성 검체 중, 3개의 검체만이 PCR-양성으로 확인되었다(25%, 3/12). 본 연구의 결과는 LAMP 기법의 폐구균 검출 해상력이 PCR 기법에 비교하여 4배 이상 높음을 보여준다(P<0.01). 결론: lytA -특이 LAMP 기법은, 중이액 내의 타 병원균과는 교차반응 없이 10 CFU 폐구균의 DNA를 검출할 수 있는 고해상 기술로서, 중이액 폐구균 검출 및 폐구균 백신의 보급에 따른 백신 효과 평가에 적용이 기대된다.