• Journal of Life Science
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• v.22 no.7
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• pp.958-963
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• 2012

The antioxidant activities of 6 solvent extracts of Artemisia capillaris were evaluated in a dintroflurobenzen (DNFB)-induced allergic mouse model. In vitro antioxidant activities were determined using DPPH and the FRAP test. Methanol (DPPH: 85.87%, FRAP: 1.772) and $dH_2O$ (DPPH: 60.69%, FRAP: 3.185) extracts showed the highest antioxidant activities compared with other solvents (ethyl acetate 41.81%, 0.407, hexane 8.37%, 0.328, etc.). In addition, we tested atopic dermatitis (AD)-like skin lesions in mice treated with DNFB. The methanol extract of A. capillaris on the AD-like skin lesions in DNFB-induced atopy inhibited ear thickness increases (47%) and the skin lesions (45%) compared with a positive control (methanol). The results suggest that they have potential as natural antioxidants and allergy-improving substances and that they may be valuable materials in the functional food or cosmeceutical industry.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.4
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• pp.377-385
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• 2016

In order to investigate the possibility of Sesamum indicum L. (S. indicum) extract as an active ingredient for wrinkle-care cosmetics, we prepared 70% ethanolic extract of S. indicum and measured its elastase inhibitory activity and collagenase inhibitory activity. We also evaluated the effect of S. indicum extract on protein and mRNA expression of MMPs in fibroblast cell (CCD-986sk). For anti-wrinkle effects, elastase inhibition activities and collagenase inhibition activities were 37.8% and 45% at a dose of $1,000{\mu}g/mL$ of S. indicum 70% ethanol extract. For a cell viability test, measured on fibroblast cell by ethanol extract of S. indicum, results showed 96% with cell viability at $100{\mu}g/mL$ concentration. According to the results of western blot of ethanol extract from S. indicum the expression of the matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-3 (MMP-3) protein was decreased by 63%, 43%, 49% at $100{\mu}g/mL$ concentration. Reverse transcription-polymerase chain reaction (PCR) of ethanol extract from S. indicum showed that the expression of MMP-1, MMP-2, MMP-3 mRNA was decreased by 82%, 79%, 82% at $100{\mu}g/mL$ concentration. The findings suggest that 70% ethanol extract from S. indicum has potential as a cosmeceutical ingredient with anti-wrinkle effects.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.4
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• pp.293-300
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• 2009

In this study, we investigated the antioxidative activity and inhibitory effects on elastase and tyrosinase of Persicaria hydropiper L. extracts. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities ($FSC_{50}$) of ethyl acetate fractions of Persicaria hydropiper L. was $5.23\;{\mu}g/mL$. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of some Persicaria hydropiper L. extracts on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system were investigated using the luminol - dependent chemiluminescence assay. The ROS scavenging activities ($OSC_{50}$) of ethyl acetate fractions of Persicaria hydropiper L. was $0.40\;{\mu}g/mL$. The protective effects of extract / fractions of Persicaria hydropiper L. on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Persicaria hydropiper L. extracts suppressed photohemolysis in a concentration dependent manner ($1\;{\sim}\;10\;{\mu}g/mL$). Inhibitory effects ($IC_{50}$) on tyrosinase of aglycone fraction of Persicaria hydropiper L. extracts was $8.90\;{\mu}g/mL$. Inhibitory effects ($IC_{50}$) on elastase of aglycone fraction of Persicaria hydropiper L. extracts was $2.37\;{\mu}g/mL$. These results indicate that extract / fractions of Persicaria hydropiper L. can function as antioxidants in biological systems, particularly skin exposed to UV radiation by anti-oxidative activity and protect cellular membranes against ROS. Persicaria hydropiper L. extract / fractions could be used as a new cosmeceutical for whitening and anti-wrinkle products.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.39 no.3
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• pp.205-213
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• 2013

This study was to improve cosmetical activity of thiamine di-lauryl sulfate (TDS) by encapsulation of nanoparticle with lecithin. Results showed that most of the nanoparticles containing the TDS were well formed in round shape with below 150 ~ 200 nm diameter as well as they were fairly stable in various pH ranges by measuring zeta potentials. The nanoparticles of TDS resulted in 85% cell viability of human normal fibroblast cells (CCD-986sk) when added at the highest concentration (1.0 mg/mL). The nanoparticles of Acer mono sap showed highest free radical scavengering effect as 88.1% in adding sample (1.0 mg/mL), compared to TDS solution of non-encapsulation (81.6%). The nanoparticles of TDS reduced the expression of MMP-1 on UV-irradiated CCD-986sk cells down to as 41.4%. The TDS solution and nanoparticles showed significant anti-microbial activities agaionst the salmonella typhimurium and listeria monocytogenes at 5 and 6 days as compared with control. Anti-microbial activities of TDS nanoparticles were similar to positive control. These results indicated that TDS nanoparticles may be a source for functional cosmetic agents capable of improving cosmetical activity such as antioxidant, whitening, and anti-wrinkling effects and can be further developed as natural preservative in cosmetics.

• Journal of Life Science
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• v.32 no.12
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• pp.938-946
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• 2022

This study aims to evaluate the effects of antioxidant activities, protein and mRNA expressions of matrix metalloproteinase (MMP) -1 and procollagen type I C-peptide (PIP) in 70% ethanol extract from Hydnocarpus anthelmintica Pierre (HE). DPPH and ABTS⁺ radicals scavenging assays were measured for antioxidant activities and HE had 73.5% and 74.4% of scavenging activities at 1,000 ㎍/ml concentration, respectively. And we investigated the inhibition of collagenase by HE, and the result was a 78.8% inhibition effect on concentrations of 1,000 ㎍/ml. In addition, an MTT assay was performed to confirm the toxicity of the CCD-986sk fibroblasts to the HE, and as a result, the cell viability rate was about 91.7% at a concentration of 50 ㎍/ml or less, and subsequent cell experiments were performed at a concentration of 50 ㎍/ml or less. We treated the cells with UVB (20 mJ/cm²) for stimulation, treated HE at various concentrations, and performed ELISA tests and RT-PCR experiments. And HE increased the PIP and mRNA in a dose-dependent manner and showed an expression rate of about 64.2% and 83.4%, respectively, at a concentration of 50 ㎍/ml compared with Cont (50.3% and 45.8%, respectively). And HE suppressed the MMP-1 protein and mRNA in a dose-dependent manner and showed a low expression rate of about 48.7% and 35.9%, respectively, at a concentration of 50 ㎍/ml. These results can be applied to developing anti-wrinkle materials for functional food and cosmetics with HE.

• Journal of Applied Biological Chemistry
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• v.58 no.2
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• pp.125-129
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• 2015

In this study, the antioxidant and anti-wrinkling effects of fraction from Vitex trifolia L. were investigated. Among the fractions, ethyl acetate fraction showed the highest antioxidant activities in 1-1-diphenyl-2-picryl-hydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoine-6-sulfonic acid) radical scavenging and elastase inhibition with 76, 89, and 74%, respectively, at a concentration of $1,000{\mu}g/mL$. This fraction, at the concentration of $25{\mu}g/mL$, inhibited 70% fibroblast cell viability and 86% the matrix metalloprotease (MMP)-1. In addition, the results from Western blot assay showed that this fraction ($25{\mu}g/mL$) expressed the MMP-1 protein level by decreasing 50%. The findings suggest that the ethyl acetate fraction from V. trifolia has great potential as a cosmeceutical ingredient with antioxidant and anti-wrinkling effects.

• Journal of Applied Biological Chemistry
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• v.54 no.2
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• pp.108-113
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• 2011

The tyrosinase inhibitory activity of extracts from Phellodendron amurense was examined. Tyrosinase inhibitory activity of 60% ethanol extracts was determined as 25% and the inhibitory activity of 60% ethanol extracts against melanin biosynthesis in melanoma cell (B16F10) was 31.2%. The purified inhibitory compounds against tyrosinase by Sephadex LH-20, MCI-gel CHP-20 column chromatography from P. amurense was confirmed as obacunone by $^1H$-NMR, $^{13}C$-NMR and Fast atom bombardment (FAB)-Mass spectrum. The tyrosinase inhibitory activities of purified obacunone was respectively as 35.1%. The safety of essence with tyrosinase inhibitory compounds from P. amurense was also assayed by various safety profiles. First, pH and viscosity change of essence for 60 days were not detected. The essence also showed the stability against temperature and light for 60 days. All these findings suggest that extracts from P. amurense has a great potential as a cosmeceutical ingredient, which has a potent whitening effect.

• Ocean and Polar Research
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• v.41 no.4
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• pp.289-309
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• 2019

Marine microalgae have long been used as food additives and feeds for juvenile fish and invertebrates as their nutritional content is beneficial for humans and marine aquaculture species. Recently, they have also been recognized as a promising source for cosmeceutical, nutraceutical, and pharmaceutical products as well as biofuels. Marine microalgae of various species are rich in multiple anti-oxidant phytochemicals and their bioactive components have been employed in cosmetics and dietary supplements. Oil contents in certain groups of marine microalgae are extraordinarily rich and abundant and therefore have been commercialized as omega-3 and omega-6 fatty acid supplements and mass production of microalgae-based biodiesels has been demonstrated by diverse research groups. Numerous natural products from marine microalgae with significant biological activities are reported yearly and this is attributed to their unique adaptive abilities to the great diversity of marine habitats and harsh conditions of marine environments. Previously unknown toxin compounds from red tide-forming dinoflagellates have also been identified which opens up potential applications in the blue biotechnology sector. This review paper provides a brief overview of the biotechnological potentials of Korean marine microalgae. We hope that this review will provide guidance for future marine biotechnology R&D strategies and the various marine microalgae-based industries in Korea.

• The Korea Journal of Herbology
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• v.31 no.4
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• pp.53-60
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• 2016

Objective : The purpose of this study was to investigate anti-aging and antioxidant effects of extracts of Nelumbo nucifera leaves (NN-L) using ethanol on skin .Methods : Each part of leaves(NN-L), flowers(NN-F) and stem(NN-S) was extracted with 70% ethanol. We performed radical scavenging assay(DPPH, ABTS+, Superoxide anion radical), elastase inhibition assay, collagenase inhibition assay. NN-L extracts were tested for cell viability(MTT assay), MMP-1 inhibition and MMP-1 protein expression on CCD-986sk cells (human fibroblast line).Results : Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. We measured its free radical scavenging activity, elastase inhibitory activity and expression of MMP-1 (matrix metalloprotease-1) in human fibroblast cells. Among the parts of Nelumbo nucifera, NN-L showed the highest antioxidant activities and in radical scavenging. DPPH, ABTS+ and Superoxide anion radical scavenging activity of NN-L at concentration of 1,000 μg/mL were 91.43%, 99.31% and 73.7% respectively. In vitro elastase and collagenase inhibition effects of NN-L at concentration of 1,000 μg/mL was 42.8% and 55.3% respectively. The ethanol extract of NN-L showed cell viability of 95.4% in 50 μg/mL concentration. In addition, The results from Western blot assay showed that NN-L decreased the expression of MMP-1 protein in a dose-dependent manner (by up to 35.0% at 50 μM).Conclusion : The findings suggest that the NN-L great potential as a cosmeceutical ingredient with antioxidant and anti-wrinkling effects.

• Journal of the Korean Applied Science and Technology
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• v.37 no.1
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• pp.28-37
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• 2020

Ginseng berry contains a large amount of Ginsenoside Re and has anti-inflammatory, anticancer, hypoglycemic and whitening effects. In this study, Rhizopus Oligosporus strain was used to establish ginseng berry fermentation process and cosmetic pharmacological activity of ginseng berry fermented product was analyzed.. The electron donating ability of ginseng berry extract by fermentation shown 81% at 1,000 ㎍/mL concentration. The ABTS⁺ radical scavenging ability of shown 100.2% at 1,000 ㎍/mL concentration. The tyrosinase inhibitory effect which is related to skin-whitening, was 57% at the concentration of 1,000 ㎍/mL. The elastase inhibitory effect which is related to skin-wrinkle, was 47% at 1,000 ㎍/mL concentration. Also, the collagenase inhibition effect was 33% at 1,000 ㎍/mL concentration. From these results, ginseng berry extracts by fermentation is considered to have anti-inflammatory, anti-wrinkle effect and whitening effect. Therefor, ginseng berry fermented product is expected to be very useful as an anti-inflammatory and anti-aging cosmetic raw material.