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http://dx.doi.org/10.5352/JLS.2022.32.12.938

MMP-1 and PIP Expressions from Ethanol Extract of Hydnocarpus anthelmintica Pierre in Human Fibroblast Cells  

Choi, Eun-Young (Department of Cosmeceutical Science, Daegu Haany University)
Jang, Young-Ah (Convergence Research Center for Smart Healthcare of KS R&DB Foundation, Kyungsung University)
Ki, Se-Gie (Department of Pharmaceutical Engineering, Daegu Catholic University)
Publication Information
Journal of Life Science / v.32, no.12, 2022 , pp. 938-946 More about this Journal
Abstract
This study aims to evaluate the effects of antioxidant activities, protein and mRNA expressions of matrix metalloproteinase (MMP) -1 and procollagen type I C-peptide (PIP) in 70% ethanol extract from Hydnocarpus anthelmintica Pierre (HE). DPPH and ABTS+ radicals scavenging assays were measured for antioxidant activities and HE had 73.5% and 74.4% of scavenging activities at 1,000 ㎍/ml concentration, respectively. And we investigated the inhibition of collagenase by HE, and the result was a 78.8% inhibition effect on concentrations of 1,000 ㎍/ml. In addition, an MTT assay was performed to confirm the toxicity of the CCD-986sk fibroblasts to the HE, and as a result, the cell viability rate was about 91.7% at a concentration of 50 ㎍/ml or less, and subsequent cell experiments were performed at a concentration of 50 ㎍/ml or less. We treated the cells with UVB (20 mJ/cm2) for stimulation, treated HE at various concentrations, and performed ELISA tests and RT-PCR experiments. And HE increased the PIP and mRNA in a dose-dependent manner and showed an expression rate of about 64.2% and 83.4%, respectively, at a concentration of 50 ㎍/ml compared with Cont (50.3% and 45.8%, respectively). And HE suppressed the MMP-1 protein and mRNA in a dose-dependent manner and showed a low expression rate of about 48.7% and 35.9%, respectively, at a concentration of 50 ㎍/ml. These results can be applied to developing anti-wrinkle materials for functional food and cosmetics with HE.
Keywords
Hydnocarpus anthelmintica Pierre; matrix metalloproteinase-1; ntioxidant; procollagen type I C-peptide;
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