Browse > Article
http://dx.doi.org/10.15230/SCSK.2016.42.4.377

A Study on the Anti-wrinkle Activities of Sesamum indicum L. Ethanol Extracts on CCD-986sk  

Joo, Da-Hye (Department of Herbal Cosmetic Science, Hoseo University)
Yoo, Dan-Hee (Department of Herbal Cosmetic Science, Hoseo University)
Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.42, no.4, 2016 , pp. 377-385 More about this Journal
Abstract
In order to investigate the possibility of Sesamum indicum L. (S. indicum) extract as an active ingredient for wrinkle-care cosmetics, we prepared 70% ethanolic extract of S. indicum and measured its elastase inhibitory activity and collagenase inhibitory activity. We also evaluated the effect of S. indicum extract on protein and mRNA expression of MMPs in fibroblast cell (CCD-986sk). For anti-wrinkle effects, elastase inhibition activities and collagenase inhibition activities were 37.8% and 45% at a dose of $1,000{\mu}g/mL$ of S. indicum 70% ethanol extract. For a cell viability test, measured on fibroblast cell by ethanol extract of S. indicum, results showed 96% with cell viability at $100{\mu}g/mL$ concentration. According to the results of western blot of ethanol extract from S. indicum the expression of the matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-3 (MMP-3) protein was decreased by 63%, 43%, 49% at $100{\mu}g/mL$ concentration. Reverse transcription-polymerase chain reaction (PCR) of ethanol extract from S. indicum showed that the expression of MMP-1, MMP-2, MMP-3 mRNA was decreased by 82%, 79%, 82% at $100{\mu}g/mL$ concentration. The findings suggest that 70% ethanol extract from S. indicum has potential as a cosmeceutical ingredient with anti-wrinkle effects.
Keywords
Sesamum indicum L.; anti-wrinkle; MMP-1; MMP-2; MMP-3;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 H. S. Lee and S. H. Kim, Safety evaluation of black garlic extract for development of cosmeceutical ingredients-skin irritation and sensitization studies, J. Korean Soc. Food Sci. Nutr., 39(8), 1213 (2010).   DOI
2 M. Takahashi, Image analysis of skin surface contour, Acta Derm. Venereol. Suppl. (Stockh), 185, 9 (1994).
3 J. Uitto, M. J. Fazio, and D. R. Olsen, Molecular mechanisms of cutaneous aging. Age-associated connective tissue alterations in the dermis, J. Am. Acad. Dermatol., 21(3), 614 (1989).   DOI
4 I. M. Braverman and E. Fonferko, Studies in cutaneous aging, Soc. Invest. Dermatol.. 78(5), 444 (1982).   DOI
5 M. J. Lee and K. H. kim, The comparison in the physicochemical properties of sesame seeds by producing areas, J. Korean Sou. Appl. Biol. Chem., 48(2), 128 (2005).
6 S. N. Ryu and E. J. Lee, Recent studies of physicochemical properties in sesame oil, Korea J. Intl. Agri., 13(3), 207 (2001).
7 Y. A. Song and H. S. Ahn, Influence in application of sesame seed oil to the skin on a change in the aging and dry skin condition, The Korea Society for Aesthetics and Cosmetology, 9(4), 103 (2011).
8 G. S. Sim, J. H. Kim, Y. Na, D. H. Lee, B. C. Lee, Y. H. Zhang, and H. B. Pyo, Anti-oxidative and inhibitory effect of Saussurea involucrata on MMP-1 in UVA-irradiated human dermal fibroblast, J. Soc. Cosmet. Sci. Korea, 31(4), 329 (2005).
9 G. J. Fisher, H. S. Talwar, J. Lin, P. Lin, F. McPhillips, Z. Wang, X. Li, Y. Wan, S. Kang, and J. J. Voorhees, Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo, J. Clin. Invest., 101(6), 1432 (1998).   DOI
10 G. S. Sim, J. H. Kim, D. H. Lee, S. M. Park, H. B. Pyo, Y. H. Zhang, and B. C. Lee, Effects or the Spatholobi caulis extract on antioxidation and inhibition of matrix metalloproteinase in human skin fibroblasts, Korean Soc. Biotechnol. Bioeng. J., 20(1), 40 (2005).
11 R. J. Cannell, S. J. Kellam, A. M. Owsianka, and J. M. Walker, Results of a large scale screen of microalgae for the production of protease inhibitors, Planta Med., 54(1), 10 (1988).   DOI
12 H. J. Park, S. M. Ock, H. J. Kim, H. J. Park, Y. B. Lee, J. M. Choi, C. S. Cho, J. Y. Lee, B. K. Cho, and D. H. Cho, Vitamin C attenuates ERK signaling to inbihit the regulation of collagen production by LL-37 in human dermal fibroblasts, Exp. Dermatol., 19(8), 258 (2010).   DOI
13 P. T. Pugliese, The skin, free radicals, and oxidative stress, Dermatol. Nurs., 7(6), 361 (1995).
14 G. L. Fisher, H. S. Talwar, J. Lin, P. Lin, F. McPhillips, Z. Wang, X. Li, Y. Wan, S. Kang, and J. J. Voorhees, Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo, J. Clin. Invest., 101(6), 1432 (1998).   DOI
15 S. N. Ryu, E. J. Lee, H. S. Yoon, and S. S. Kang, Chemical structure and physiological activity of lignan component in sesame, Korea J. Crop Sci., 48, 65 (2003).
16 Y. H. Kim, Differences of antioxidant contents and antioxidative activity of sesame seeds by producing areas, Kor. J. Hort. Sci. Technol., 44(6), 846 (2003).
17 E. Wuensch and H. G. Heidrich, On the quantitative determination of collagenase, Hoppe Seylers Z. Physiol. Chem., 333, 149 (1963).   DOI
18 J. Carmichael, W. G. DeGraff, A. F. Gazdar, J. D. Minna, and J. B. Mitchell, Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of radiosensitivity, Cancer Res., 47(4), 943 (1987).
19 S. Y. Lee, J. H. An, H. Chun, and H. Y. Cho, Isolation and characterization of MMP-1 inhibitor peptide from Crataegus pinnatifida bunge in fibroblast cellline HS68 cells, J. Appl. Biol. Chem., 46(1), 60 (2003).
20 Y. Matsumura and H. N. Ananthaswamy, Toxic effects of ultraviolet radiation on the skin, Toxicol. Appl. Pharmacol., 195(3), 298 (2004).   DOI
21 S. G. Park, S. N. Kim, J. C. Lee, H. S. Kim, Y. J. Kim, B. G. Lee, and I. S. Jang, Anti-aging effect on skin with Jaeum-Dan (JED), Kor. J. Herbology., 19(1), 67 (2004).
22 G. J. Fisher, S. C. Datta, H. S. Talwar, Z. Q. Wang, J. Varani, S. Kang, and J. J. Voorhees, Molecular basis of sun-induced premature skin ageing and retinoid antagonism, Nature, 379, 35 (1996).   DOI
23 H. J. Moon, S. R. Lee, S. N. Shim, S. H. Jeong, V. A. Stonik, V. A. Rasskazov, T. Zvyagintseva, and Y. H. Lee, Fucoidan inhibits UVB-induced MMP-1 expression in human skin fibroblasts, Biol. Pharm. Bull., 31(2), 284 (2008).   DOI
24 L. Rittie and G. J. Fisher, UV-light-induced signal cascades and skin aging, Ageing Res. Rev., 1(4), 705 (2002).   DOI
25 E. A. Weiss, Oilseed crops, Longman, London, 31, (1983).
26 S. J. Park and M. H. Kang, Functional properties of sesame seed, Food Ind. Nutr., 9(2), 31 (2004).
27 P. Dhar, K. Chattopadhyay, D. Bhattacharyya, and S. Ghosh, Antioxidative effect of sesame lignans in diabetes mellitus blood : an in vitro study. J. Oleo. Sci., 54(1), 39 (2005).   DOI
28 H. Ukeda, S. Maeda, T. Ishii, and M. Sawamura, Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3'-{1-[(phenylamino)-carbonyl]-3, 4-tetrazolium}-bis (4-methoxy-6-nitro) benzenesulfonic acid hydrate reduction by xanthine-xanthine oxidase, Anal. Biochem., 251(2), 206 (1997).   DOI
29 N. M. Kim, B. S. Koo, S. G. Lee, E. I. Hwang, S. H. So, and J. H. Do, Effect of Korean red ginseng on collagen biosynthesis and MMP-I activity in human dermal fibroblast, J. Ginseng Res., 31(2), 86 (2007).   DOI
30 B. Yang, C. Ji, J. Kang, W. Chen, Z. Bi, and Y. Wan, Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts, Int. J. Mol. Med., 23(4), 555 (2009).
31 H. Nagase and J. F. Woessner, Matrix metalloproteinases, J. Biol. Chem., 274(31), 21491 (1999).   DOI