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http://dx.doi.org/10.3839/jabc.2015.022

Antioxidant and Anti-wrinkling Effects of Extracts from Vitex trifolia L.  

Lee, Mi-Kyoung (Korea Promotion Institute for Traditional Medicine Industry)
Kim, Dong-Hee (Korea Promotion Institute for Traditional Medicine Industry)
Park, Tae-Soon (Korea Promotion Institute for Traditional Medicine Industry)
Son, Jun-Ho (Korea Promotion Institute for Traditional Medicine Industry)
Publication Information
Journal of Applied Biological Chemistry / v.58, no.2, 2015 , pp. 125-129 More about this Journal
Abstract
In this study, the antioxidant and anti-wrinkling effects of fraction from Vitex trifolia L. were investigated. Among the fractions, ethyl acetate fraction showed the highest antioxidant activities in 1-1-diphenyl-2-picryl-hydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoine-6-sulfonic acid) radical scavenging and elastase inhibition with 76, 89, and 74%, respectively, at a concentration of $1,000{\mu}g/mL$. This fraction, at the concentration of $25{\mu}g/mL$, inhibited 70% fibroblast cell viability and 86% the matrix metalloprotease (MMP)-1. In addition, the results from Western blot assay showed that this fraction ($25{\mu}g/mL$) expressed the MMP-1 protein level by decreasing 50%. The findings suggest that the ethyl acetate fraction from V. trifolia has great potential as a cosmeceutical ingredient with antioxidant and anti-wrinkling effects.
Keywords
1-1-diphenyl-2-picryl-hydrazyl; 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); anti-wrinkle; elastase; matrix metalloprotease-1; Vitex trifolia L.;
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