Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Biotechnological Potential of Korean Marine Microalgal Strains and Its Future Prospectives

  • Hong, Ji Won (Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea) ;
  • Kang, Nam Seon (Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea) ;
  • Jang, Hyeong Seok (Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea) ;
  • Kim, Hyung June (Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea) ;
  • An, Yong Rock (Department of Exhibition and Education, National Marine Biodiversity Institute of Korea) ;
  • Yoon, Moongeun (Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea) ;
  • Kim, Hyung Seop (Faculty of Marine Applied Bioscience, College of Ocean Science and Engineering, Kunsan National University)
  • Received : 2019.10.17
  • Accepted : 2019.12.09
  • Published : 2019.12.30
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Abstract

Marine microalgae have long been used as food additives and feeds for juvenile fish and invertebrates as their nutritional content is beneficial for humans and marine aquaculture species. Recently, they have also been recognized as a promising source for cosmeceutical, nutraceutical, and pharmaceutical products as well as biofuels. Marine microalgae of various species are rich in multiple anti-oxidant phytochemicals and their bioactive components have been employed in cosmetics and dietary supplements. Oil contents in certain groups of marine microalgae are extraordinarily rich and abundant and therefore have been commercialized as omega-3 and omega-6 fatty acid supplements and mass production of microalgae-based biodiesels has been demonstrated by diverse research groups. Numerous natural products from marine microalgae with significant biological activities are reported yearly and this is attributed to their unique adaptive abilities to the great diversity of marine habitats and harsh conditions of marine environments. Previously unknown toxin compounds from red tide-forming dinoflagellates have also been identified which opens up potential applications in the blue biotechnology sector. This review paper provides a brief overview of the biotechnological potentials of Korean marine microalgae. We hope that this review will provide guidance for future marine biotechnology R&D strategies and the various marine microalgae-based industries in Korea.

Keywords

References

  1. Affan A, Karawita R, Jeon Y, Lee J (2007) Growth characteristics and antioxidant properties of the benthic diatom Navicula incerta (Bacillariophyceae) from Jeju Island, Korea. J Phycol 43:823-832 https://doi.org/10.1111/j.1529-8817.2007.00367.x
  2. Ariede MB, Candido TM, Jacome ALM, Velasco MVR, de Carvalho JCM, Baby AR (2017) Cosmetic attributes of algae: A review. Algal Res 25:483-487 https://doi.org/10.1016/j.algal.2017.05.019
  3. Arrigo KR (2005) Marine microorganisms and global nutrient cycles. Nature 437:349-355 https://doi.org/10.1038/nature04159
  4. Assuncao J, Guedes A, Malcata FX (2017) Biotechnological and pharmacological applications of biotoxins and other bioactive molecules from dinoflagellates. Mar Drugs 15:393. doi:10.3390/md15120393
  5. Bae SY, Yim JH, Lee HK, Pyo S (2006) Activation of murine peritoneal macrophages by sulfated exopolysaccharide from marine microalga Gyrodinium impudicum (strain KG03): Involvement of the $NF-{\kappa}B$ and JNK pathway. Int Immunopharmacol 6:473-484 https://doi.org/10.1016/j.intimp.2005.09.009
  6. Belay A, Yishimichi O, Kazuyuki M, Hidenori S (1993) Current knowledge on potential health benefits of Spirulina. J Appl Phycol 5:235-241 https://doi.org/10.1007/BF00004024
  7. Brinza L, Dring MJ, Gavrilescu M (2007) Marine microand macro-algal species as biosorbents for heavy metals. Environ Eng Manag J 6:237-251 https://doi.org/10.30638/eemj.2007.029
  8. Brooks G, Franklin S, Avila J, Decker SM, Baliu E, Rakitsky W, Piechocki J, Zdanis D, Norris LM (2017) Novel microbial food compositions. Korean patent No. 101769121, 10 Aug 2017
  9. Camacho FG, Rodriguez JG, Miron AS, Garcia MC, Belarbi EH, Chisti Y, Grima EM (2007) Biotechnological significance of toxic marine dinoflagellates. Biotechnol Adv 25:176-194 https://doi.org/10.1016/j.biotechadv.2006.11.008
  10. Cancela A, Maceiras R, Garcia B, Alfonsin V, Sanchez A (2016) Harvesting and lipids extraction of Pavlova lutheri. Environ Prog Sustain 35:1842-1847 https://doi.org/10.1002/ep.12411
  11. Cardozo KH, Guaratini T, Barros MP, Falcao VR, Tonon AP, Lopes NP, Campos S, Torres MA, Souza AO, Colepicolo P, Pinto E (2007) Metabolites from algae with economical impact. Comp Biochem Phys C 146:60-78 https://doi.org/10.1016/j.cbpb.2006.09.003
  12. Champenois J, Marfaing H, Pierre R (2015) Review of the taxonomic revision of Chlorella and consequences for its food uses in Europe. J Appl Phycol 27:1845-1851 https://doi.org/10.1007/s10811-014-0431-2
  13. Cho MJ, Jung HS, Song MY, Seo HH, Kulkarni A, Suh SS, Lee TK, Moh SH (2014) Effect of sun screen utilizing Porphyra-334 derived from ocean algae for skin protection. J Korea Acad Industr Coop Soc 15:4272-4278 https://doi.org/10.5762/KAIS.2014.15.7.4272
  14. Cho S, Lee N, Park S, Yu J, Luong TT, Oh YK, Lee T (2013) Microalgae cultivation for bioenergy production using wastewaters from a municipal WWTP as nutritional sources. Bioresource Technol 131:515-520 https://doi.org/10.1016/j.biortech.2012.12.176
  15. Cho SH, Lee GA, Yun JH (2018) Feed composition in the formulated feed for abalone. Korean patent No. 101879776, 18 Jul 2018
  16. Cho Y (2004) Variation of filtration rate of the short-necked clam, Ruditapes philippinarum, with concentration of suspended solid. M.D. Thesis, Yosu National University, 92 p
  17. Choi G, Bae M, Park J, Park B, Ahn C, Oh H (2007) Growth and ${\gamma}$-linolenic acid production of Arthrospira (Spirulina platensis) in heterotrophic culture. Kor J Microbiol Biotechn 35:45-51
  18. Choi JW, Hwang CJ, Lee HP, Kim HS, Han B, Hong JT (2017) Inhibitory effect of ethanol extract of Nannochloropsis oceanica on lipopolysaccharide-induced neuroinflammation, oxidative stress, amyloidogenesis and memory impairment. Oncotarget 8:45517-45530 https://doi.org/10.18632/oncotarget.17268
  19. Choi YJ, Jo WS, Kim HJ, Nam BH, Kang EY, Oh SJ, Lee GA, Jeong MH (2010) Anti-inflammatory effect of Chlorella ellipsoidea extracted from seawater by organic solvents. Korean J Fish Aquat Sci 43:39-45 https://doi.org/10.5657/kfas.2010.43.1.039
  20. Choi YS, Sang BI, Um YS, Lee KY, Shin JH, Jung YH, Hong DE, Yoo BS (2011) A method for culturing microalgae by using organic waste resources. Korean patent No. 101061035, 25 Aug 2011
  21. Choo M (2014) The microalgae culture using commercial compound fertilizer. M.D. Thesis, Soonchunhyang University, 43 p
  22. Cloern JE, Foster SQ, Kleckner AE (2014) Phytoplankton primary production in the world's estuarine-coastal ecosystems. Biogeosciences 11:2477-2501 https://doi.org/10.5194/bg-11-2477-2014
  23. Craggs RJ, McAuley PJ, Smith VJ (1997) Wastewater nutrient removal by marine microalgae grown on a corrugated raceway. Water Res 31:1701-1707 https://doi.org/10.1016/S0043-1354(96)00093-0
  24. Correa FDN, Luna AS, da Costa ACA (2017) Kinetics and equilibrium of lanthanum biosorption by free and immobilized microalgal cells. Adsorp Sci Technol 35:137-152 https://doi.org/10.1177/0263617416672667
  25. Darienko T, Gustavs L, Mudimu O, Menendez CR, Schumann R, Karsten U, Friedl T, Proschold T (2010) Chloroidium, a common terrestrial coccoid green alga previously assigned to Chlorella (Trebouxiophyceae, Chlorophyta). Eur J Phycol 45:79-95 https://doi.org/10.1080/09670260903362820
  26. Darienko T, Proschold T (2015) Genetic variability and taxonomic revision of the genus Auxenochlorella (Shihira et Krauss) Kalina et Puncocharova (Trebouxiophyceae, Chlorophyta). J Phycol 51:394-400 https://doi.org/10.1111/jpy.12279
  27. Day AG, Dillon HF, Brooks G, Franklin S (2016) Direct chemical modification of microbial biomass and microbial oils. Korean patent No. 101607235, 23 Mar 2016
  28. Dolatabadi JEN, de la Guardia M (2011) Applications of diatoms and silica nanotechnology in biosensing, drug and gene delivery, and formation of complex metal nanostructures. Trac-Trend Anal Chem 30:1538-1548 https://doi.org/10.1016/j.trac.2011.04.015
  29. Ebenezer V, Ki JS (2016) Toxic effects of Aroclor 1016 and bisphenol A on marine green algae Tetraselmis suecica, diatom Ditylum brightwellii and dinoflagellate Prorocentrum minimum. Korean J Microbiol 52:306-312 https://doi.org/10.7845/kjm.2016.6050
  30. Food and Agriculture Organization of the United Nations (2016) The state of world fisheries and aquaculture contributing to food security and nutrition for all. Food and Agriculture Organization of the United Nations, Rome, 200 p
  31. Food and Agriculture Organization of the United Nations (2018) The state of world fisheries and aquaculture. Food and Agriculture Organization of the United Nations, Rome, 210 p
  32. Fukatsu T, Onodera KI, Ohta Y, Oba Y, Nakamura H, Shintani T, Yoshioka Y, Okamoto T, ten Lohuis M, Miller DJ, Kawachi M (2007) Zooxanthellamide D, a polyhydroxy polyene amide from a marine dinoflagellate, and chemotaxonomic perspective of the Symbiodinium polyols. J Nat Prod 70:407-411 https://doi.org/10.1021/np060596p
  33. Go GM (2017a) Method for massive culture of adhesive microalgae. Korean patent No. 101810782, 13 Dec 2017
  34. Go GM (2017b) Method of separating silica from adhesive diatoms isolated from Jeju magma sea water and silica separated using the same. Korean patent No. 101788507, 13 Oct 2017
  35. Go GM (2018) Method for red sea cucumber aquaculture using adhesive microalgae. Korean patent No. 101868687, 11 Jun 2018
  36. Go S, Lee S, Jeong G, Kim S (2012) Factors affecting the growth and the oil accumulation of marine microalgae, Tetraselmis suecica. Bioproc Biosyst Eng 35:145-150 https://doi.org/10.1007/s00449-011-0635-7
  37. Griffiths M, Harrison ST, Smit M, Maharajh D (2016) Major commercial products from micro-and macroalgae. In: Faizal B, Yusuf C (eds) Algae biotechnology. Springer, Amsterdam, pp 269-300
  38. Guillerme JB, Couteau C, Coiffard L (2017) Applications for marine resources in cosmetics. Cosmetics 4:35. doi:10.3390/cosmetics4030035
  39. Guiry MD, Guiry GM (2019) AlgaeBase. National University of Ireland. http://www.algaebase.org Accessed 8 July 2019
  40. Han HK, Hur SB (2000) Dietary value of benthic diatoms for newly settled abalone, Haliotis discus hannai Ino. Korean Soc Fish Aquat Sci 13:153-161
  41. Han JJ (2016) Method for culturing mycoplasma contamination free cells and method for eliminating mycoplasma contamination of cells. Korean patent No. 101620922, 9 May 2016
  42. Hempel F, Bozarth AS, Lindenkamp N, Klingl A, Zauner S, Linne U, Steinbuchel A, Maier UG (2011) Microalgae as bioreactors for bioplastic production. Microb Cell Fact 10:81. doi:10.1186/1475-2859-10-81
  43. Hur SB (2015) Culture and application of plankton live food in aquaculture. Life Science Publishing Co., Seoul, 28 p
  44. Hur SB, Bae JH (2013) Novel Chlorella vulgaris capable low temperature growth and use thereof. Korean patent No. 101323957, 24 Oct 2013
  45. Hur SB, Bae JH, Youn JY, Jo MJ (2015) KMMCC-Korea Marine Microalgae Culture Center: list of strains. Algae 30:S1-S188
  46. Hur SB, Bae JH, Ko SK (2018) Feed composition for culturing marine invertebrate larvae. Korean patent No.101871507, 20 Jun 2018
  47. Hutchins DA, Mulholland MR, Fu F (2009) Nutrient cycles and marine microbes in a $CO_{2}$-enriched ocean. Oceanography 22:128-145 https://doi.org/10.5670/oceanog.2009.103
  48. Hwang BS, Yoon EY, Kim HS, Yih W, Park JY, Jeong HJ, Rho J (2013) Ostreol A: A new cytotoxic compound isolated from the epiphytic dinoflagellate Ostreopsis cf. ovata from the coastal waters of Jeju Island, Korea. Bioorg Med Chem Lett 23:3023-3027 https://doi.org/10.1016/j.bmcl.2013.03.020
  49. Hwang BS, Kim HS, Yih W, Jeong EJ, Rho JR (2014) Acuminolide A: structure and bioactivity of a new polyether macrolide from dinoflagellate Dinophysis acuminata. Org Lett 16:5362-5365 https://doi.org/10.1021/ol502567g
  50. Hwang BS (2015) Isolation and structure determination of new bioactive compounds originated from marine sponge and toxic dinoflagellate species. Ph.D. Thesis, Kunsan National University, 152 p
  51. Hwang BS, Yoon EY, Jeong EJ, Park J, Kim E, Rho J (2018) Determination of the absolute configuration of polyhydroxy compound ostreol B isolated from the dinoflagellate Ostreopsis cf. ovata. J Org Chem 83:194-202 https://doi.org/10.1021/acs.joc.7b02569
  52. Im SG, Yang J, Yoo Y, Nam K, Lim J, Park MS (2015) Method for cultivation of microalgae using wastewater pretreated by polymer film. Korean patent No. 101484732, 14 Jan 2015
  53. Inoue K, Khunathai K, Kawakita H, Ohto K, Kurata K, Fukuda H, Atsumi K (2018) Recovery of precious metals using microalgae waste. Adv Env Was Mana Rec 1:1-10
  54. Intellectual Property On (2015) Patent and market analysis report - Application techniques of marine microalgae and microorganisms-derived useful substances. Intellectual Property On, Seoul, 57 p
  55. International Organization for Standardization (1995) Water quality-marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum. ISO 10253
  56. Jang HS, Kang NS, Kim KM, Jeon BH, Park JS, Hong JW (2017) Description and application of a marine microalga Auxenochlorella protothecoides isolated from Ulleung-do. J Life Sci 27:1152-1160 https://doi.org/10.5352/JLS.2017.27.10.1152
  57. Jang SH, Jeong HJ, Kwon JE (2017) High contents of eicosapentaenoic acid and docosahexaenoic acid in the mixotrophic dinoflagellate Paragymnodinium shiwhaense and identification of putative omega-3 biosynthetic genes. Algal Res 25:525-537 https://doi.org/10.1016/j.algal.2017.06.020
  58. Jeon CH, Park CK, Lim JH, Ryu YJ, Yang JH, Shim SH, Cho YH, Kim KH, Park H, Kim JH, Park J, Jung I, Kang SM, Shin DW, Lim SM, Lee CG, Na BK (2017) A study on the fuel quality characteristics and cold weather performance test for biodiesel derived from microalgae. Trans. Korean Hydrog New Energy Soc 28:545-553
  59. Jeon JY, Moon BH, An BK, Lim SU, Park JH (2012) Process for preparing feed additives comprising fermented Chlorella and process for preparing feed for breeding chicken using the same. Korean patent No. 101156940, 11 Jun 2012
  60. Jeon JY, Moon BH, An BK, Lim SU (2013) Feed additives for producing milk having high amount of milk proteins and process for the preparation of feed for lactating dairy cattle using the same. Korean patent No. 101252132, 2 Apr 2013
  61. Jeon JY (2016) Possibility of heavy metal (Cu and Zn) removal in the contaminated sediments using LED and microalgae. M.D. Thesis, Pukyong National University, 140 p
  62. Jeon YJ, Ko SC, Lee JS, Kim DK, Kim KN (2014a) Novel antioxidative peptide purified from a marine Chlorella ellipsoidea. Korean patent No. 101440684, 4 Sep 2014
  63. Jeon Y, Ko S, Lee J, Kim D, Kim K (2014b) Antihypersensitive peptide from Chlorella ellipsoidea. Korean patent No. 101404850, 30 May 2014
  64. Jeong HJ, Kim JS, Yoo YD, Kim ST, Song JY, Kim TH, Seong KA, Kang NS, Kim MS, Kim JH, Kim S, Ryu J, Lee HM, Yih WH (2008) Control of the harmful alga Cochlodinium polykrikoides by the naked ciliate Strombidinopsis jeokjo in mesocosm enclosures. Harmful Algae 7:368-377 https://doi.org/10.1016/j.hal.2007.12.004
  65. Jeong HJ, Lee KH, Kim JH, Lim AS, Jang SH (2017) Inhibitor comprising dinoflagellates Alexandrium spp. against scuticuciliates. Korean patent No. 101793393, 27 Oct 2017
  66. Jeong HJ, Lee SY, Kang NS, Yoo YD, Lim AS, Lee MJ, Kim HS, Yih W, Yamashita H, LaJeunesse TC (2014) Genetics and morphology characterize the dinoflagellate Symbiodinium voratum, n. sp., (Dinophyceae) as the sole representative of Symbiodinium clade E. J Eukaryot Microbiol 61:75-94 https://doi.org/10.1111/jeu.12088
  67. Jeong HJ, Park KH, Kim JS, Kang HJ, Kim CH, Choi HJ, Kim YS, Park JY, Park MG (2003) Reduction in the toxicity caused by a toxic dinoflagellate Gymnodinium catenatum by the feeding of the heterotrophic dinoflagellate Polykrikos kofoidii. Aquat Microb Ecol 31:307-312 https://doi.org/10.3354/ame031307
  68. Jeong HJ, Yoo YD, Kang NS, Lim AS, Seong KA, Lee SY, Lee MJ, Lee KH, Kim HS, Shin W, Nam SW (2012) Heterotrophic feeding as a newly identified survival strategy of the dinoflagellate Symbiodinium. P Natl Acad Sci USA 109:12604-12609 https://doi.org/10.1073/pnas.1204302109
  69. Jeong HJ, Yoo YD, Kang NS, Rho JR, Seong KA, Park JW, Nam GS, Yih WH (2010) Ecology of Gymnodinium aureolum. I. Feeding in western Korean water. Aquat Microb Ecol 59:239-255 https://doi.org/10.3354/ame01394
  70. Jeong HJ, Yoo YD, Park JY, Song JY, Kim ST, Lee SH, Kim KY, Yih WH (2005) Feeding by the phototrophic red-tide dinoflagellates: Five species newly revealed and six species previously known to be mixotrophic. Aquat Microb Ecol 40:133-155 https://doi.org/10.3354/ame040133
  71. Jha D, Jain V, Sharma B, Kant A, Garlapati VK (2017) Microalgae-based pharmaceuticals and nutraceuticals: An emerging field with immense market potential. ChemBioEng Rev 4:257-272 https://doi.org/10.1002/cben.201600023
  72. Jiang L, Luo S, Fan X, Yang Z, Guo R (2011) Biomass and lipid production of marine microalgae using municipal wastewater and high concentration of $CO_{2}$. Appl Energy 88:3336-3341 https://doi.org/10.1016/j.apenergy.2011.03.043
  73. Jo HJ (2016) The optimum growth conditions and mixotrophy of a toxic benthic dinoflagellate Prorocentrum lima. M.D. Thesis, Kunsan National University, 56 p
  74. Ju X, Igarashi K, Miyashita SI, Mitsuhashi H, Inagaki K, Fujii SI, Sawada H, Kuwabara T, Minoda A (2016) Effective and selective recovery of gold and palladium ions from metal wastewater using a sulfothermophilic red alga, Galdieria sulphuraria. Bioresource Technol 211:759-764 https://doi.org/10.1016/j.biortech.2016.01.061
  75. Jung HJ (2016) Preparation and characterization of cphycocaynin loaded nanoemulsion systems. M.D. Thesis, Korea University, 58 p
  76. Jung HS, Cho MJ, Seo HH, Kulkarni A, Suh SS, Lee TK, Moh SH (2014) Role of shinorine derived from microalgae in skin protection. J Korea Acad Industr Coop Soc 15:4416-4422 https://doi.org/10.5762/KAIS.2014.15.7.4416
  77. Jung MH, Lee SS, Kim HA, Hwang HJ (2018) Comparative study on the effects of six species of microalgae extracts on hair loss prevention and scalp improvement. J Life Sci 28:345-350 https://doi.org/10.5352/JLS.2018.28.3.345
  78. Kalina T, Puncocharova M (1987) Taxonomy of the subfamily Scotiellocystoideae Fott 1976 (Chlorellaceae, Chlorophyceae). Algol Stud 45:473-521
  79. Kang CH (2015a) Studies on biomass and oil production from eight microalgae using light emitting diode (LED). M.D. Thesis, Pukyong National University, 37 p
  80. Kang M (2015b) Comparison of planktonic microalgae growth using LED lights. M.D. Thesis, Chonnam National University, 54 p
  81. Kang DH, Abu A, Lee DW, Park HS, Kim HJ (2013a) Method of Spirulina culturing using the medium composition for Spirulina culturing. Korean patent No. 101256434, 12 Apr 2013
  82. Kang M, Lim SY, Lee C, Baek HR, Shin J (2017) Comparison of marine microalgae growth using LED lights. J Korean Soc Fish Mar Sci Edu 29:552-559
  83. Kang NS, Jeong HJ, Moestrup O, Shin W, Nam SW, Park JY, De Salas MF, Kim KW, Noh JH (2010) Description of a new planktonic mixotrophic dinoflagellate Paragymnodinium shiwhaense n. gen., n. sp. from the coastal waters off western Korea: Morphology, pigments, and ribosomal DNA gene sequence. J Eukaryot Microbiol 57:121-144 https://doi.org/10.1111/j.1550-7408.2009.00462.x
  84. Kang NS, Jeong HJ, Lee SY, Lim AS, Lee MJ, Kim HS, Yih, W (2013b) Morphology and molecular characterization of the epiphytic benthic dinoflagellate Ostreopsis cf. ovata in the temperate waters off Jeju Island, Korea. Harmful Algae 27:98-112 https://doi.org/10.1016/j.hal.2013.05.006
  85. Kang SH (2016) Utilization of swine manure for microalgae cultivation. M.D.Thesis, Pukyong National University, 39 p
  86. Kim B (2012a) Optimization of microalgae (Dunaliella tertiolecta, Tetraselmis chui, Nannochloropsis oculata) culture conditions for biodiesel production. M.D. Thesis, Chosun University, 80 p
  87. Kim MK (2012b) Recent trends in microalgae biotechnology. Bioin Special Webzine No. 27, Biotech Policy Research Center, 13 p
  88. Kim J (2014a) Solvent extraction of fucoxanthin from Phaeodactylum tricornutum. Sep Sci Technol 49:410-415 https://doi.org/10.1080/01496395.2013.839703
  89. Kim JS (2014b) Extraction of antifungal substances through the mass culture of Amphidnium carterae. M.D. Thesis, Pukyong National University, 74 p
  90. Kim HM (2017) A study on the anti-photoaging effects of Nannochloropsis oceanica extracts and their active component in UVB-irradiated human dermal fibroblasts. M.D. Thesis, Konkuk University, 46 p
  91. Kim NK (2013) Oil production from microalgae and development of persister cell inhibitors. M.D. Thesis, Pukyong National University, 66 p
  92. Kim BH, Park MW, Myeong JI, Kim TI, Jo JK, Son MH (2014) Culture medium of food organisms for diatom. Korean patent No. 101382384, 1 Apr 2014
  93. Kim D, Jeong H, Lee K, Park H, Lee K (2005) A Study on the odor reduction of food waste leachate by some microorganisms. J Korea Org Resour Recycl Assoc 13:91-97
  94. Kim EK, Choi GG, Kim HS, Ahn CY, Oh HM (2012) Increasing ${\gamma}$-linolenic acid content in Spirulina platensis using fatty acid supplement and light-dark illumination. J Appl Phycol 24:743-750 https://doi.org/10.1007/s10811-011-9693-0
  95. Kim GY, Heo J, Kim HS, Han JI (2017a). Bicarbonatebased cultivation of Dunaliella salina for enhancing carbon utilization efficiency. Bioresource Technol 237:72-77 https://doi.org/10.1016/j.biortech.2017.04.009
  96. Kim H, Oh H, Jeong JH, Lee S, Moon H, Jeong Y (2017b) Functional evaluation of marine micro-algae Amphidinium carterae extract. Korean J Food Preserv 24:673-679 https://doi.org/10.11002/KJFP.2017.24.5.673
  97. Kim HS, Jo S, Rho J, Yih W, Lim HC, Kim J, Park JW, Park HW, Hwang BS, Ha N (2015a) Teleaulax amphioxeia, the cryptophycean strain as a live-feed for mariculture. Korean patent No. 101576838, 7 Dec 2015
  98. Kim J, Jung J, Lee J, Kim K, Choi TO, Kim J, Jeon YJ, Kwon EE (2016a) Pyrogenic transformation of Nannochloropsis oceanica into fatty acid methyl esters without oil extraction for estimating total lipid content. Bioresource Technol 212:55-61 https://doi.org/10.1016/j.biortech.2016.04.024
  99. Kim JH, Jeong HJ, Lim AS, Kwon JE, Lee KH, Park KH, Kim HS (2017c) Removal of two pathogenic scuticociliates Miamiensis avidus and Miamiensis sp. using cells or culture filtrates of the dinoflagellate Alexandrium andersonii. Harmful Algae 63:133-145 https://doi.org/10.1016/j.hal.2017.02.002
  100. Kim KM, Kang NS, Jang HS, Park JS, Jeon BH, Hong JW (2017d) Characterization of Heterochlorella luteoviridis (Trebouxiaceae, Trebouxiophyceae) isolated from the Port of Jeongja in Ulsan, Korea. J Mar Biosci Biotechnol 9:22-29 https://doi.org/10.15433/KSMB.2017.9.2.022
  101. Kim KW, Yang DJ, Moh SH, Choi YH, Seo HH, Lee JH, Kim HS (2018a) Wound healing composition for skin external application comprising mycosporine-like amino acid and method for preparing the same. Korean patent No. 101833895, 23 Feb 2018
  102. Kim M, Nam SW, Shin W, Coats DW, Park MG (2015b) Fate of green plastids in Dinophysis caudata following ingestion of the benthic ciliate Mesodinium coatsi: Ultrastructure and psbA gene. Harmful Algae 43:66-73 https://doi.org/10.1016/j.hal.2015.02.004
  103. Kim MH, Lee CK, Kim HS, Kim SY, Lee WG, Yim J, Kim S, Kang P (2013) Pharmaceutical composition for prevention or treatment of influenza virus infection comprising sulfated exopolysaccharide as an active ingredient. Korean patent No. 101333785, 21 Nov 2013
  104. Kim SJ, Jeong HJ, Jang SH, Lee SY, Park TG (2017e) Interactions between the voracious heterotrophic nanoflagellate Katablepharis japonica and common heterotrophic protists. Algae 32:309-324 https://doi.org/10.4490/algae.2017.32.11.27
  105. Kim SM, Pan CH, Koo SY, Mok IK, Um JI (2018b) Fucoxanthin fine powder based on beta cyclodextrin and method for manufacturing the same. Korean patent No. 101876505, 3 Jul 2018
  106. Kim SM, Pan CH, Koo SY, Mok IK, Um JI (2018c) Chitosan-coated fucoxanthin fine powder based on casein and method for manufacturing the same. Korean patent No. 101875571, 2 Jul 2018
  107. Kim SM, Koo SY, Pan CH, Mok IK, Shin BK, Um JI (2018d) Fucoxanthin fine powder based on casein and method for manufacturing the same. Korean patent No. 101876507, 3 Jul 2018
  108. Kim SM, Koo SY, Pan C, Mok IK, Shin BK, Um JI (2018e) Fucoxanthin fine powder based on whey protein method for manufacturing the same. Korean patent No. 101876506, 3 Jul 2018
  109. Kim Y, Ha S, Kim DU, Shin I (2017f) Antibacterial activity of ethanol extracts from marine micro-algae. Korean J Food Sci Technol 49:390-395 https://doi.org/10.9721/KJFST.2017.49.4.390
  110. Kim ZH, Park H, Ryu YJ, Shin DW, Hong SJ, Tran HL, Lim SM, Lee CG (2015c) Algal biomass and biodiesel production by utilizing the nutrients dissolved in seawater using semi-permeable membrane photobioreactors J Appl Phycol 27:1763-1773 https://doi.org/10.1007/s10811-015-0556-y
  111. Kim ZH, Park H, Lee CG (2016b) Seasonal assessment of biomass and fatty acid productivity by Tetraselmis sp. in the ocean using semi-permeable membrane photobioreactors. J Microbiol Biotechn 26:1098-1102 https://doi.org/10.4014/jmb.1601.01031
  112. Knothe G (2009) Improving biodiesel fuel properties by modifying fatty ester composition. Energ Environ Sci 2:759-766 https://doi.org/10.1039/b903941d
  113. Ko K, Lee C, Moon H, Lee Y, Yang J, Cho K, Kim D, Yeo I (2016) Simultaneous effect of salinity and temperature on the neutral lipid and starch accumulation by oceanic microalgae Nannochloropsis granulata and Chlorella vulgaris. J Korean Soc Mar Env Energ 19:236-245 https://doi.org/10.7846/JKOSMEE.2016.19.3.236
  114. Ko SC, Kang N, Kim EA, Kang MC, Lee SH, Kang SM, Lee JB, Jeon BT, Kim SK, Park SJ, Park PJ (2012a) A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from a marine Chlorella ellipsoidea and its antihypertensive effect in spontaneously hypertensive rats. Process Biochem 47:2005-2011 https://doi.org/10.1016/j.procbio.2012.07.015
  115. Ko SC, Kim D, Jeon YJ (2012b) Protective effect of a novel antioxidative peptide purified from a marine Chlorella ellipsoidea protein against free radical-induced oxidative stress. Food Chem Toxicol 50:2294-2302 https://doi.org/10.1016/j.fct.2012.04.022
  116. Korea Institute of Ocean Science and Technology (2018) A planning research for the development and commercialization of marine bio promising materials. Korea Institute of Ocean Science and Technology, Busan, 89 p
  117. Kumar KS, Dahms HU, Won EJ, Lee JS, Shin KH (2015) Microalgae-a promising tool for heavy metal remediation. Ecotox Environ Saf 113:329-352 https://doi.org/10.1016/j.ecoenv.2014.12.019
  118. Lane CD, Coury DA, Allnutt FT (2017) Composition and potential products from Auxenochlorella protothecoides, Chlorella sorokiniana and Chlorella vulgaris. Ind Biotechnol 13:270-276 https://doi.org/10.1089/ind.2017.0015
  119. Lee C, Kim HS, Nam JR, Lee M, Yim J, Lee HK, De Clercq E (2009a) Anti-picornavirus activity and other antiviral activity of sulfated exopolysaccharide from the marine microalga Gyrodinium impudicum strain KG03. Antivir Res 82:A40. doi:10.1016/j.antiviral.2009.02.084
  120. Lee CG, Lee SM, Lim SM, Jo YH, Ryu Y, Shin DW, Jeon HN, Park HW (2016a) A novel Phaeodactylum tricornutum strain and a method for producing biodiesel using the same. Korean patent No. 101678076, 15 Nov 2016
  121. Lee H, Shin W, Jung J, Kim CW, Lee JW, Kwon J, Yang J (2015a) Optimization of variables affecting the direct transesterification of wet biomass from Nannochloropsis oceanica using ionic liquid as a co-solvent. Bioproc Biosyst Eng 38:981-987 https://doi.org/10.1007/s00449-014-1343-x
  122. Lee H, Kim KH, Mun SC, Chang YK, Choi SQ (2018) A new method to produce cellulose nanofibrils from microalgae and the measurement of their mechanical strength. Carbohyd Polym 180:276-285 https://doi.org/10.1016/j.carbpol.2017.09.104
  123. Lee HK, Yim J, Kim S, Ahn SH, Lee YK (2005) Gyrodinium impudicum KG03 strain (KCTC 10325BP) and a method for production of sulfated exopolysaccharide having antiviral effect therefrom. Korean patent No. 100481678, 29 Mar 2005
  124. Lee HK, Yim J, Kim S (2006) Immunostimulating composition containing sulfated exopolysaccharide (p-KG03) produced by Gyrodinium impudicum KG03 strain (KCTC 10325BP) therefrom. Korean patent No. 100623460, 6 Sep 2006
  125. Lee HS (2005) Changes in EPA and DHA content of marine microalgae at different environment condition. M.D. Thesis, Gyeongsang National University, 58 p
  126. Lee IK (2001) Algae in Korea: Ecology and application. Academy Books, Seoul, 350 p
  127. Lee J, Kim B (2002) Growth characteristics of five microalgal species isolated from Jeju Island and four microalgal stock strains in hatchery. Algae 17:117-125 https://doi.org/10.4490/ALGAE.2002.17.2.117
  128. Lee KT, Lim SJ (2018a) Feed additive including organic compounds in biofloc and method for production thereof. Korean patent No. 101854016, 25 Apr 2018
  129. Lee KT, Lim SJ (2018b) Feed stuff for sea cucumber including organic compounds in biofloc and method for preparation thereof. Korean patent No. 101822736, 22 Jan 2018
  130. Lee KH, Jeong HJ, Kwon JE, Kang HC, Kim JH, Jang SH, Park JY, Yoon EY, Kim JS (2016b) Mixotrophic ability of the phototrophic dinoflagellates Alexandrium andersonii, A. affine, and A. fraterculus. Harmful Algae 59:67-81 https://doi.org/10.1016/j.hal.2016.09.008
  131. Lee M (2014) Characterization of heat shock protein genes from the marine diatom Ditylum brightwellii and their transcriptional responses to environmental stressors. M.D. Thesis, Sangmyung University, 96 p
  132. Lee M, Guo R, Ki J (2014a) Different transcriptional responses of heat shock protein 20 in the marine diatom Ditylum brightwellii exposed to metals and endocrinedisrupting chemicals. Environ Toxicol 29:1379-1389 https://doi.org/10.1002/tox.21868
  133. Lee SE, Shin HC (2015) The influence of water temperature on filtration rates and ingestion rates of the blue mussel, Mytilus galloprovincialis (Bivalvia). Korean J Malacol 31:203-212 https://doi.org/10.9710/kjm.2015.31.3.203
  134. Lee SH, Chang DU, Lee BJ, Jeon YJ (2009b) Antioxidant activity of solubilized Tetraselmis suecica and Chlorella ellipsoidea by enzymatic digests. J Food Sci Nutr 14:21-28
  135. Lee S, Go S, Jeong G, Kim S (2011) Oil production from five marine microalgae for the production of biodiesel. Biotechnol. Bioproc E 16:561-566 https://doi.org/10.1007/s12257-010-0360-0
  136. Lee S, Hwang BS, Kim HS, Yih W, Jeong EJ, Rho J (2015b) A new diol ester derivative of dinophysistoxin-1 from cultures of Prorocentrum lima collected in South Korea. Bull Korean Chem Soc 36:395-398 https://doi.org/10.1002/bkcs.10031
  137. Lee SH, Lee JE, Kim Y, Lee SY (2016c) The production of high purity phycocyanin by Spirulina platensis using light-emitting diodes based two-stage cultivation. Appl Biochem Biotech 178:382-395 https://doi.org/10.1007/s12010-015-1879-5
  138. Lee SY, Lee JE, Lee SH (2016d) The production of high purity phycocyanin from cyanobacterium Spirulina platensis using LEDs based two-stage cultivation. Korean patent No. 101657943, 9 Sep 2016
  139. Lee S (2011) Studies on biomass and oil content of marine microalgae for biodiesel production. M.D. Thesis, Pukyong National University, 40 p
  140. Lee SB (2016) Isolation and structure determination of bioactive secondary metabolites from benthic marine dinoflagellates and sponges. M.D. Thesis, Kunsan National University, 77 p
  141. Lee SD, Park JS, Lee JH (2013) Taxonomic study of the genus Achnanthes (Bacillariophyta) in Korean coastal waters. J Ecol Environ 36:391-406 https://doi.org/10.5141/ecoenv.2013.391
  142. Lee SM, Cho Y, Shin D, Jeon H, Ryu YJ, Lim S, Lee C (2014b) Investigation of cultivation and FAME composition isolated Phaeodactylum tricornutum from Youngheung island. J Mar Biosci Biotechnol 6:47-52 https://doi.org/10.15433/ksmb.2014.6.2.047
  143. Lim KH (2002) Variation of filtration rate of the shortnecked clam, Ruditapes philippinarum, Adams and Reeve with temperature and salinity. M.D. Thesis, Yosu National University, 100 p
  144. Lim KH, Jang KS, Kim IS, Lee JH, Shin HC (2008) The Influence of water temperature and salinity on filtration rates of the hard clam, Meretrix petechialis. Korean J Malacol 24:175-188
  145. Matsunaga T, Takeyama H, Nakao T, Yamazawa A (1999) Screening of marine microalgae for bioremediation of cadmium-polluted seawater. J Biotechnol 70:33-38 https://doi.org/10.1016/S0168-1656(99)00055-3
  146. Min B, Park H, Hur SB (2006) Grazing of brackish water cyclopoid copepod Paracylopina nana on different microalgae species and concentration. J Aquaculture 19:310-314
  147. Ministry of Future Creation and Science (2015) Mass culture of microalgae and application for the development of early aquaculture feed and extraction of high value products. Ministry of Future Creation and Science, Seoul, 36 p
  148. Ministry of Oceans and Fisheries of Korea (2019) 1st administrative master plan for marine bioresources. http://www.mof.go.kr/article Accessed 17 Jan 2019
  149. Miranda AF, Ramkumar N, Andriotis C, Holtkemeier T, Yasmin A, Rochfort S, Wlodkowic D, Morrison P, Roddick F, Spangenberg G, Lal B (2017) Applications of microalgal biofilms for wastewater treatment and bioenergy production. Biotechnol Biofuels 10:120. doi:10.1186/s13068-017-0798-9
  150. Mishra M, Arukha AP, Bashir T, Yadav D, Prasad GB (2017) All new faces of diatoms: Potential source of nanomaterials and beyond. Front Microbiol 8:1239. doi:10.3389/fmicb.2017.01239
  151. Moh SH, Jung DH, Lee JH, Seo HH, Jung HS, Cho MJ, Lee HE, Shin DS, Lee YR, Lee JH, Kim HS (2015) Anti-UV composition for skin external application comprising peptide derivatives from extract of microalgae comprising the same. Korean patent No. 101552010, 3 Sep 2015
  152. Moh SH, Suh SS, Cho MJ, Song MY, Hwang J, Park M, Lee TK (2014) Evaluation of Sun protection factor (SPF) and protection factor of UVA (PFA) of the sunscreen containing microalgal extracts and MAAs. J Korea Acad Industr Coop Soc 15:3312-3318 https://doi.org/10.5762/KAIS.2014.15.5.3312
  153. Moore JK, Doney SC, Glover DM, Fung IY (2001) Iron cycling and nutrient limitation patterns in surface waters of the world ocean. Deep-Sea Res Pt II 49:463-508 https://doi.org/10.1016/S0967-0645(01)00109-6
  154. Monteiro CM, Carlos PML (2012) Metal uptake by microalgae: underlying mechanisms and practical applications. Biotechnol Progr 28:299-311 https://doi.org/10.1002/btpr.1504
  155. Neustupa J, Nemcova Y, Elieas M, Skaloud P (2009) Kalinella bambusicola gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel coccoid Chlorella-like subaerial alga from Southeast Asia. Phycol Res 57:159-169 https://doi.org/10.1111/j.1440-1835.2009.00534.x
  156. Oh S, Kwon H, Jeon J, Yang H (2015) Effect of monochromatic light emitting diode on the growth of four microalgae species (Chlorella vulgaris, Nitzschia sp., Phaeodactylum tricornutum, Skeletonema sp.). J Korean Soc Mar Environ Saf 21:1-8 https://doi.org/10.7837/kosomes.2015.21.1.001
  157. Onodera KI, Nakamura H, Oba Y, Ohizumi Y, Ojika M (2005) Zooxanthellamide Cs: Vasoconstrictive polyhydroxylated macrolides with the largest lactone ring size from a marine dinoflagellate of Symbiodinium sp. J Am Chem Soc 127:10406-10411 https://doi.org/10.1021/ja050810g
  158. Onodera KI, Nakamura H, Oba Y, Ojika M (2004) Zooxanthellamide B, a novel large polyhydroxy metabolite from a marine dinoflagellate of Symbiodinium sp. Biosci Biotech Bioch 68:955-958 https://doi.org/10.1271/bbb.68.955
  159. Paniagua-Michel J (2015) Microalgal nutraceuticals. In: Kim SK (ed) Handbook of marine microalgae, Academic Press, Cambridge, pp 255-267
  160. Park HW (2012) The selection of the cryptophyte Teleaulax amphioxeia strains for a prey organism of rotifer and bivalve larvae. M.D.Thesis, Kunsan National University, 41 p
  161. Park HW, Kim HS, Jo S (2016) Growth performance of the rotifer Brachionus plicatilis and the larvae of two bivalves fed on the cryptophyte Teleaulax amphioxeia. Korean J Fish Aquat Sci 49:351-358 https://doi.org/10.5657/KFAS.2016.0351
  162. Park J, Lee G, Kim K, Kim K, Choi S, Jeong M, Oh Y (2014) Microalgal oil recovery by solvent extraction from Nannochloropsis oceanica. Korean Chem Eng Res 52:88-91 https://doi.org/10.9713/kcer.2014.52.1.88
  163. Park JG, Park YS (1999) Comparison of morphological characteristics and the 24S rRNA sequences of Cochlodinium polykrikoides and Gyrodinium impudicum. J Kor Soc Oce 4:363-370
  164. Park MG, Kim S, Kim HS, Myung G, Kang YG, Yih W (2006) First successful culture of the marine dinoflagellate Dinophysis acuminata. Aquat Microb Ecol 45:101-106 https://doi.org/10.3354/ame045101
  165. Park TG, Lim WA, Park YT, Lee CK, Jeong HJ (2013) Economic impact, management and mitigation of red tides in Korea. Harmful Algae 30:S131-S143 https://doi.org/10.1016/j.hal.2013.10.012
  166. Patel A, Matsakas L, Rova U, Christakopoulos P (2018) Heterotrophic cultivation of Auxenochlorella protothecoides using forest biomass as a feedstock for sustainable biodiesel production. Biotechnol Biofuels 11:169. doi:10.1186/s13068-018-1173-1
  167. Piechocki J, Zdanis D, Norris LM, Rakitsky W, Klamczynska B (2018) Lipid-rich microalgal flour food compositions. Korean patent No. 101849367, 10 Apr 2018
  168. Polle JE, Struwe L, Jin E (2008) Identification and characterization of a new strain of the unicellular green alga Dunaliella salina (Teod.) from Korea. J Microbiol Biotechn 18:821-827
  169. Ra CH, Kang CH, Kim NK, Lee CG, Kim SK (2015) Cultivation of four microalgae for biomass and oil production using a two-stage culture strategy with salt stress. Renew Energ 80:117-122 https://doi.org/10.1016/j.renene.2015.02.002
  170. Ra C, Kang C, Jung J, Jeong G, Kim S (2016) Effects of light-emitting diodes (LEDs) on the accumulation of lipid content using a two-phase culture process with three microalgae. Bioresource Technol 212:254-261 https://doi.org/10.1016/j.biortech.2016.04.059
  171. Rawat I, Gupta SK, Shriwastav A, Singh P, Kumari S, Bux F (2016) Microalgae applications in wastewater treatment. In: Faizal B, Yusuf C (eds) Algae biotechnology. Springer, Amsterdam, pp 249-268
  172. Rho JR, Jeong EJ, Yih W, Kim HS (2016) Acuminolide A from Dinophysis acuminata and Method for isolating the same and composition for preventing or treating muscle weakness diseases comprising the same. Korean patent No. 101656285, 5 Sep 2016
  173. Ryu BM, Himaya SWA, Kim S (2015) Applications of microalgae-derived active ingredients as cosmeceuticals In: Kim SK (ed) Handbook of marine microalgae, Academic Press, Cambridge, pp 309-316
  174. Ryu HS (2011) Study of the salinity acclimation in Dunaliella. M.D. Thesis, Hanyang University, 53 p
  175. Satake M, Cornelio K, Hanashima S, Malabed R, Murata M, Matsumori N, Zhang H, Hayashi F, Mori S, Kim JS, Kim CH (2017) Structures of the largest amphidinol homologues from the dinoflagellate Amphidinium carterae and structure-activity relationships. J Nat Prod 80:2883-2888 https://doi.org/10.1021/acs.jnatprod.7b00345
  176. Seong KA, Jeong HJ, Kim S, Kim GH, Kang JH (2006) Bacterivory by co-occurring red-tide algae, heterotrophic nanoflagellates, and ciliates on marine bacteria. Mar Ecol-Prog Ser 322:85-97 https://doi.org/10.3354/meps322085
  177. Shah MMR, Samarakoon KW, Ko J, Lakmal HC, Lee J, An S, Jeon Y (2014) Potentiality of benthic dinoflagellate cultures and screening of their bioactivities in Jeju Island, Korea. Afr J Biotechnol 13:792-805 https://doi.org/10.5897/AJB2013.13250
  178. Shah MMR, Samarakoon KW, An S, Jeon YJ, Lee J (2016) Growth characteristics of three benthic dinoflagellates in mass culture and their antioxidant properties. J Fish Aquat Sci 11:268-277 https://doi.org/10.3923/jfas.2016.268.277
  179. Shen N, Birungi ZS, Chirwa EM (2017) Selective biosorption of precious metals by cell-surface engineered microalgae. Chem Engineer Trans 61:25-30
  180. Shim C (2010) Screening of microalgae for biodiesel and optimal culture condition. M.D. Thesis, Soonchunhyang University, 62 p
  181. Slade R, Bauen A (2013) Micro-algae cultivation for biofuels: cost, energy balance, environmental impacts and future prospects. Biomass Bioenerg 53:29-38 https://doi.org/10.1016/j.biombioe.2012.12.019
  182. Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101:87-96 https://doi.org/10.1263/jbb.101.87
  183. Suh SS, Hwang J, Park M, Seo HH, Kim HS, Lee JH, Moh SH, Lee TK (2014) Anti-inflammation activities of mycosporine-like amino acids (MAAs) in response to UV radiation suggest potential anti-skin aging activity. Mar Drugs 12:5174-5187 https://doi.org/10.3390/md12105174
  184. Tang D, Di B, Wei G, Ni IH, Wang S (2006) Spatial, seasonal and species variations of harmful algal blooms in the South Yellow Sea and East China Sea. Hydrobiologia 568:245-253 https://doi.org/10.1007/s10750-006-0108-1
  185. Trimbur DE, Im C, Dillon HF, Day AG, Franklin S, Coragliotti A (2015) Production of oil in microorganisms. Korean patent No. 101523255, 20 May 2015
  186. Venkatesan J, Manivasagan P, Kim SK (2015) Marine microalgae biotechnology: present trends and future advances. In: Kim SK (ed) Handbook of marine microalgae, Academic Press, Cambridge, pp 1-9
  187. Wang J, Chen C (2009) Biosorbents for heavy metals removal and their future. Biotechnol Adv 27:195-226 https://doi.org/10.1016/j.biotechadv.2008.11.002
  188. Whitton R, Ometto F, Pidou M, Jarvis P, Villa R, Jefferson B (2015) Microalgae for municipal wastewater nutrient remediation: Mechanisms, reactors and outlook for tertiary treatment. Environ Technol Rev 4:133-148 https://doi.org/10.1080/21622515.2015.1105308
  189. WoRMS (2019) World register of marine species. http://www.marinespecies.org accessed 8 July 2019
  190. Yang AR, Lee S, Yoo YD, Kim HS, Jeong EJ, Rho J (2017) Limaol: A Polyketide from the benthic marine dinoflagellate Prorocentrum lima. J Nat Prod 80:1688-1692 https://doi.org/10.1021/acs.jnatprod.7b00127
  191. Yang AR (2017) Isolation and structure determination of bioactive compounds from two marine dinoflagellates. M.D.Thesis, Kunsan National University, 59 p
  192. Yih W, Rho JR, Kim HS (2011) Methods for massive culture of Dinophysis acuminata and isolation of pectenotoxin-2. Korean patent No. 101055594, 3 Aug 2011
  193. Yim JH (2002) A study on the characterization and production of exopolysaccharide produced by marine microalgae Gyrodinium impudicum strain KG03. Ph.D. Thesis, Kyung Hee University, 334 p
  194. Yim JH, Kim SJ, Ahn SH, Lee HG (2003) Optimal conditions for the production of sulfated polysaccharide by marine microalga Gyrodinium impudicum strain KG03. Biomol Eng 20:273-280 https://doi.org/10.1016/S1389-0344(03)00070-4
  195. Yim JH, Lee HG (2004) Axenic culture of Gyrodinium impudicum strain KG03, a marine red-tide microalga that produces exopolysaccharide. J Microbiol 42:305-314
  196. Yim JH, Kim SJ, Ahn SH, Lee CK, Rhie KT, Lee HK (2004) Antiviral effects of sulfated exopolysaccharide from the marine microalga Gyrodinium impudicum strain KG03. Mar Biotechnol 6:17-25 https://doi.org/10.1007/s10126-003-0002-z
  197. Yim JH, Son E, Pyo S, Lee HK (2005) Novel sulfated polysaccharide derived from red-tide microalga Gyrodinium impudicum strain KG03 with immunostimulating activity in vivo. Mar Biotechnol 7:331-338 https://doi.org/10.1007/s10126-004-0404-6
  198. Yoo YD, Jeong HJ, Kang NS, Song JY, Kim KY, Lee KT, Kim JH (2010) Feeding by the newly described mixotrophic dinoflagellate Paragymnodinium shiwhaense: feeding mechanism, prey species, and effect of prey concentration. J Eukaryot Microbiol 57:145-158 https://doi.org/10.1111/j.1550-7408.2009.00448.x
  199. Yoon EY, Yang AR, Park J, Moon SJ, Jeong EJ, Rho JR (2017) Characterization of a new trioxilin and a sulfoquinovosyl diacylglycerol with anti-inflammatory properties from the dinoflagellate Oxyrrhis marina. Mar Drugs 15:57. doi:10.3390/md15030057