• Animal Systematics, Evolution and Diversity
• /
• v.6 no.1
• /
• pp.1-16
• /
• 1990

The skull and superficial cephalis muscles of Coreoleuciscus splendidus Mori was investigated comparatively with other some fishes of the family Cyprinidae to study on the systematic position of this species. Coreoleuciscs splendidus was unique in the morphology of sphenotic process and the urohyal and this species differ from the true bottom dwellers of the subfamily Gobioninae in the strucutre of cephalic lateral-line canals, the relation of parasphenoids with pterotic and prootic, the morphology of urohyal , basihyal , hypobranchial, and , particulary, the configuration of adductor manibulae complex. With reference to the information so far obtained , the taxonomic position of the present species was discussed in the Cyprinidae.

• Korean Journal of Ichthyology
• /
• v.24 no.4
• /
• pp.253-262
• /
• 2012

A skeletal system of Coreoleuciscus splendidus from Seomjin and Mankyeong Rivers is described and figured in detail. The osteological characteristics are as follow: preorbital area elongated; eight proximal radials on the anal fin; 1st basibranchial bone with a bottle gourd shape; two types of pharynegeal teeth; intercalarium's ascending process absent and rib 4 broad greatly. Meanwhile, the number of actinost and the length of external process and internal process on the pelvic girdle are variable with individuals. These characteristics are shown in the two population. The epural on the caudal skeleton, however, has two types only in C. splendidus of Mankyeong River.

• Korean Journal of Ichthyology
• /
• v.21 no.2
• /
• pp.129-133
• /
• 2009

Random Amplified Polymorphic DNA (RAPD) analysis was used to investigate the genetic variations of Coreoleuciscus splendidus within and among the West Korea Subdistrict populations (in Han and Geum Rivers) and the South Korea Subdistrict populations (in Seomjin and Nakdong Rivers). Twelve random primers were employed to generate RAPD markers. All primers were produced to identify specific RAPD markers between the West and South Korea Subdistrict populations. Analyses of genetic similarity and distance among the West and South Korea Subdistrict populations of C. splendidus also revealed similar results, with low genetic similarity (0.49~0.53) and high distance value (0.63~0.71). UPGMA dendrogram based on genetic distance was also similar in results. Therefore, the West Korea Subdistrict populations and the South Korea Subdistrict populations vary in genetic structure, and C. splendidus in the South Korea Subdistrict may represent a different species.

• Korean Journal of Ichthyology
• /
• v.29 no.1
• /
• pp.1-12
• /
• 2017

A natural hybrid of interspecific between the Coreoleuciscus splendidus and C. aeruginos (Cypriniformes: Cyprinidae) was captured in the Hwang-ji Stream, a tributary of the Nakdong River basin in Korea. An interspecific hybrid between C. splendidus and C. aeruginos was genetically identified based on morphological characteristics and the sequence analysis of nuclear recombination activating gene 1 (RAG1) gene (1,334 bp) and mitochondrial cytochrome c oxidase subunit 1 (CO1) gene (1,551 bp). As a result of morphological variations, the natural hybrid appeared to have an intermediate character between two parental species (C. splendidus and C. aeruginos) in three variations of black array (s) on dorsal, caudal and anal fin rays. Phylogenetic analysis inferred from RAG1 and CO1 sequence data revealed that Coreoleuciscus populations from Hwang-ji stream consist of two pure Coreoleuciscus species and a hybrid individual group. The individuals were clearly identified the cross and reciprocal hybrid by CO1 gene analysis. In RAG1 gene, 13 nucleotide variation loci were detected and the hybrid individuals displayed the double peaks of sequence chromatograms at the 9 diagnostic positions. In this study, molecular data and morphological variations were clearly demonstrated that hybridization did occur between C. splendidus and C. aeruginos. However, F2 hybrid generation and reproductive capacity of F1 hybrid individuals were not demonstrated.

• Korean Journal of Ichthyology
• /
• v.18 no.2
• /
• pp.78-86
• /
• 2006

We examined the genetic diversity in intra-populations of Korean shiner, Coreoleuciscus splendidus, in six major rivers (Bukhan, Namhan, Geum, Osipcheon, Nakdong and Seomjin river) of Korea based on two different mitochondrial genes, the mitochondrial cytochrome b and the 16S rRNA. Analysis of sequence variation in a 657-bp segment of the mitochondrial cytochrome b gene revealed deep divergence among populations (98.2~99.9%) and high genetic diversity from geographically isolated populations. Intra-specific variation in this 697-bp segment of the 16S rRNA gene sequences was very low and nearly identical. Six isolate populations of C. splendidus showed a high similarity (97.7%~99.7%). This result may be indicative of a complex history of connection and isolation of the rivers in the Korea peninsula.

• Development and Reproduction
• /
• v.23 no.2
• /
• pp.161-170
• /
• 2019

The purpose of this study is to investigate the development of Coreoleuciscus splendidus egg and larvae morphology in the Ungcheon-stream. C. splendidus eggs were round and ranged in size from 1.86-2.01 mm (mean $1.91{\pm}0.14mm$). Immediately after hatching, the larvae had egg yolk in the abdomen with a total length of 5.27-6.63 mm (mean $5.95{\pm}0.96mm$). On the 10 days after hatching, the latter was 8.44-8.65 mm (mean $8.54{\pm}0.14mm$) in total length, and 5 dorsal fin rays were formed in dorsal fin and 8 caudal fin soft rays were formed in caudal fin. At 88 days after hatching, total length was 26.2-25.7 mm (mean $25.9{\pm}0.35mm$), and scales were formed throughout the body. The fin rays of each part were iii.7 in dorsal fin and iii.6 in anal fin, and finally the external form was the same as the adult fish.

• Applied Microscopy
• /
• v.40 no.1
• /
• pp.9-14
• /
• 2010

Coreoleuciscus splendidus is a teleost belonging to Gobioninae, Cyprinidae. The oogenesis was investigated by light microscope. The ovary was located between intestine and air bladder, a grayish and ellipsoidal shape with the major axis 20 mm and the minor axis 5 mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. In conclusion, the oogenesis of C. splendidus was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilic substance in the cytoplasm. The oogenesis of C. splendidus is similar with other Cyprinidae fishes. But further study on ultrastructural study of fertilized egg envelope will be necessary to get the species specificity.

• Journal of Aquaculture
• /
• v.20 no.2
• /
• pp.140-143
• /
• 2007

Cytogenetic analyses of an endemic species, Coreoleuciscus splendidus (Cyprinidae) was performed including erythrocyte measurement, chromosome count and karyotyping, nucleolar organizing region (NOR) banding and flow cytometric analysis of genome size. C. splendidus had the same modal chromosome number of 2n = 48 between sexes, however, displayed a sex-related dimorphism in their chromosome karyotypes. Males represented a pair of heteromorphic chromosomes which couldn‘t be seen in any female individuals, indicating that the sex determination mechanism of this species should be a typical XX-XY based male heterogamety (female=10M+6SM+8A+XX vs male=10M+6SM+8A+XY). Other cytogenetic features such as Ag-NORs located in a pair of acrocentric chromosomes, estimated nuclear volume ($28{\mu}m^3$) and cellular DNA content (2.4 pg/cell) suggest that genetic recombination might be the main driving force responsible for the evolution of this species rather than the polyploidy-based evolutionary process as in many other Cyprinidae species.

• Applied Microscopy
• /
• v.39 no.3
• /
• pp.227-236
• /
• 2009

The ultrastructure of spermatogenesis and sperm in Coreoleuciscus splendidus, belonging to Gobioninae, Cyprinidae was investigated by light and electron microscopes. The testis was located between intestine and air bladder. The size of testis was major axis 1.8 cm, minor axis 3 mm. The testis of C. splendidus contained numerous testicular cysts, and spermatogenesis was non-synchronized in these testicular cysts. In May, the upper area of testis contained with other germ cells and sperm but the lower area of testis contained with matured sperm only. In case of spermatogonia, the nucleus was comparatively large spherical, and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and that of secondary spermatocyte was smaller than that of primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged in a middle piece. The head of matured sperm was a spherical shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the tail of sperm had not lateral fins and 7 outer coarse fibers.

• Applied Microscopy
• /
• v.44 no.3
• /
• pp.96-99
• /
• 2014

Study on the egg envelope of an endemic Korean freshwater fish, Coreoleuciscus splendidus was carried out by light and scanning electron microscopes during its spawning season. The egg envelope on the entire egg is filled with plenty of long cylinder-like villi before and after fertilization. The fertilized eggs consist of two regions, the attaching and non-attaching parts to stick to the substrates. The villi covering the fertilized eggs have three kinds of villi in its length: 1) normal- sized villi of an average of $10.6{\mu}m$ on the non-attachment part; 2) longer and more slender villi over about $20{\mu}m$ on the attachment part, at least being two times longer than those of the non-attachment; and 3) shorter villi under about $5.0{\mu}m$ around the micropyle, with half the size of the non-attachment villi. The micropyle rotated in a counter-clockwise direction with five pieces of the ridges.