Browse > Article

Genetic Variation of Coreoleuciscus splendidus Populations from Four Major Rivers in Korea as Assessed by RAPD PCR  

Song, Ha-Yoon (Department of Marine Biotechnology, Soonchunhyang University)
Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
Publication Information
Korean Journal of Ichthyology / v.21, no.2, 2009 , pp. 129-133 More about this Journal
Abstract
Random Amplified Polymorphic DNA (RAPD) analysis was used to investigate the genetic variations of Coreoleuciscus splendidus within and among the West Korea Subdistrict populations (in Han and Geum Rivers) and the South Korea Subdistrict populations (in Seomjin and Nakdong Rivers). Twelve random primers were employed to generate RAPD markers. All primers were produced to identify specific RAPD markers between the West and South Korea Subdistrict populations. Analyses of genetic similarity and distance among the West and South Korea Subdistrict populations of C. splendidus also revealed similar results, with low genetic similarity (0.49~0.53) and high distance value (0.63~0.71). UPGMA dendrogram based on genetic distance was also similar in results. Therefore, the West Korea Subdistrict populations and the South Korea Subdistrict populations vary in genetic structure, and C. splendidus in the South Korea Subdistrict may represent a different species.
Keywords
Coreoleuciscus splendidus; RAPD PCR; genetic variation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 김동준. 2003. 칼납자루 Acheilogonathus koreensis와 임실납자루 A. somjinesis의 분자계통학적 연구. 순천향대학교 대학원 석사학위 논문, 35pp.
2 김영자∙김일찬∙이세영∙이완옥∙조용철∙이재성. 2003. 한국산 어류 미토콘드리아 DNA의 분자계통학적 이용 및 보존. 한국육수학회지, 36: 221-234.   과학기술학회마을
3 Song, H.B. and G.M. Park. 2006. A Molecular genetic variation among intra-populations of Korean shiner, Coreoleuciscus splendidus Mori (Cyprinidae). Kor. J. Ichthyol., 18: 78-86.
4 Todd, C.D., A.M. Walker, K. Wolff, S.J. Northcott, A.F. Waler, M.G. Ritchie, R. Hoskins, R.J. Abbott and N. Hazon. 1997. Genetic differentiation of populations of the copepod sea louse Lepeophtheirus slmonis (Kroyer) ectoparasitic on wild and farmed salmonids around the coasts of Scotland: evidence from RAPD marker. J. Exp. Mar. Biol. Ecol., 210: 251- 274.   DOI   ScienceOn
5 Bardakci, F. and D.O.F. Skibinski. 1994. Application of the RAPD technique in tilapia fish, species and subspecies identification. J Hered., 73: 117-123.   DOI   ScienceOn
6 Dinesh, K.R, T.M. Lim, W. Chan and V.P.E. Phang. 1996. Genetic variation inferred from RAPD fingerprinting in three species of tilapia. Aquacult. Intl., 4: 19-30.
7 Yeh, F.C., T. Boyle, Y. Rongcai, Z. Ye and J.M. Xian. 1999. POPGENE, Version 1.31. A Microsoft Window Based Free Ware for Population Genetic Analysis., University of Alberta, Edmonton.
8 Miller, M. 1997. Tools for population genetic analysis (TFPGA) 1.3: a windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by author. http://www.Marksgenetic software.net/tfpga.htm.
9 Asahida, T.T. Kobayashi, K. Saitoh and I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffer contaunung high concentration of urea. Fish. Sci., 62: 727-730.   DOI
10 Mori, T. 1935. Studies on the geographical distribution of freshwater fish in Korea. Bull. Biogeogr. Soc. Jap., 7: 35-61.
11 Liu, Z.J., P. Li, B.J. Aegue and R.A. Dunham. 1999. Random amplified polymorphic DNA markers: usefulness for gene mapping and analysis of genetic variation of catfish. Aquaculture., 174: 59-68.   DOI   ScienceOn
12 Callejas, C. and M.D. Ochando. 2001. Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the Iberian peninsula. J. Fish Biol., 59: 1589-1599.   DOI   ScienceOn
13 Degani, G., J. Pitcovsiki, T. Dobski and Y. Plotsky. 1997. DNA fingerprint bands applied to analysis of variations in angelfish (Pterophyllum scalare) (Cichlidae) strains. J. Aquacult. in the Trop., 12: 43-51.
14 김익수∙최 윤∙이충렬∙이용주∙김병직∙김지현. 2005. 한국원색어류대도감. 교학사, 서울, pp. 39-116.