Browse > Article

Species and Hybrid Identification of Genus Coreoleuciscus Species in Hwnag-ji Stream, Nakdong River Basin in Korea  

Song, Ha-Yoon (Inland Fisheries Research Institute, National Institute of Fisheries Science)
Kim, Jae-Hun (Department of Life Science, Soonchunhyang University)
Seo, In-Young (Department of Life Science, Soonchunhyang University)
Bang, In-Chul (Department of Life Science, Soonchunhyang University)
Publication Information
Korean Journal of Ichthyology / v.29, no.1, 2017 , pp. 1-12 More about this Journal
Abstract
A natural hybrid of interspecific between the Coreoleuciscus splendidus and C. aeruginos (Cypriniformes: Cyprinidae) was captured in the Hwang-ji Stream, a tributary of the Nakdong River basin in Korea. An interspecific hybrid between C. splendidus and C. aeruginos was genetically identified based on morphological characteristics and the sequence analysis of nuclear recombination activating gene 1 (RAG1) gene (1,334 bp) and mitochondrial cytochrome c oxidase subunit 1 (CO1) gene (1,551 bp). As a result of morphological variations, the natural hybrid appeared to have an intermediate character between two parental species (C. splendidus and C. aeruginos) in three variations of black array (s) on dorsal, caudal and anal fin rays. Phylogenetic analysis inferred from RAG1 and CO1 sequence data revealed that Coreoleuciscus populations from Hwang-ji stream consist of two pure Coreoleuciscus species and a hybrid individual group. The individuals were clearly identified the cross and reciprocal hybrid by CO1 gene analysis. In RAG1 gene, 13 nucleotide variation loci were detected and the hybrid individuals displayed the double peaks of sequence chromatograms at the 9 diagnostic positions. In this study, molecular data and morphological variations were clearly demonstrated that hybridization did occur between C. splendidus and C. aeruginos. However, F2 hybrid generation and reproductive capacity of F1 hybrid individuals were not demonstrated.
Keywords
Coreoleuciscus splendidus; C. aeruginos; natural hybrid; species identification;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Ko, M.H. 2009. Reproductive mechanisms of the unisexual diploidtriploid hybrid complex of between the spine loach Cobitis hankugensis and Iksookimia longicorpa (Teleostei, Cobitis) in Korea. Doctors's thesis, Chonbuk National University, 160pp. (in Korean)
2 Kuriiwa, K., N. Hanzawa, T. Yoshino, S. Kimura and M. Nishida. 2007. Phylogenetic relationships and natural hybridization in rabbitfishes (Teleostei: Siganidae) inferred from mitochondrial and nuclear DNA analysis. Mol. Phyl. Ecol., 45: 69-80.   DOI
3 Kwan, Y.S., M.H. Ko and Y.J. Won. 2014. Genomic replacement of native Cobitis lutheri with introduced C. tetralineata through a hybrid swarm following the artificial connection of ricer systems. Ecol. Evol., 4: 1451-1465.   DOI
4 Lee, I.R., H. Yang, J.H. Kim, K.Y. Kim and I.C. Bang. 2009. Identification of a natural hybrid between the striped spine loach Cobitis tetralineata and the king spine loach Iksookimia longicorpa by analyzing mitochondrial COI and nuclear RAG1 sequence. Koran J. Ichthyol., 21: 287-290. (in Korean)
5 Lee, T.Y. and S.Y. Kim. 1987. Experimental studies on the mechanism of reproductive cycle in the bluegill, Lepomis macrochirus. Korean J. Fish. Aquat. Sci., 20: 489-500. (in Korean)
6 Lee, W.O., I.R. Lee, H.Y. Song and I.C. Bang. 2008. Genetic differentiation of the largemouth bass Micropterus salmoides from the major rivers and reservoirs in Korea assessed by AFLP. Korean J. Limnol., 41: 395-401. (in Korean)
7 Mori, T. 1935. Studies on the geographical distribution of freshwater fish in Korea. Bull. Biogeogr. Soc. Jap., 7: 35-61.
8 Yoon, Y.E., I.R. Lee, S.Y. Park, E.J. Kang, E.O. Kim, S.K. Yang, Y.K. Nam and I.C. Bang. 2009. Genetic identification of hybrids between Rhodeus uyekii and R. notatus by sequence analysis of RAG-1 gene. J. Aquacult., 22: 79-82. (in Korean)
9 Wendel, J.F. and J.J. Doyle. 1998. Phylogenetic incongruence: window into genome history and molecular evolution. In: Soltis, D.E., Soltis, P.S., Doyle, J.J. (eds.), Molecular systematics of plants II: DNA sequencing. Kluwer academic publishers, Boston, pp. 265-296.
10 Wu, G., J. Luo, K. Chen, C. Xian, G. Lin, Z. Wang, Z. Lin, J. Luo and K. Pan. 1990. Analysis and comparison of morphology and cytology between the F sub (1) hybrid catfish (Claris fuscusxC. lazera) and its parents. Acta Hydrobiologica Sinica, Wnhan., 14: 328-335.
11 Huelsenbeck, J.P. and F.R. Ronquise. 2001. MRBYES: bayesian inference of phylogeny. Bio-informatics, 17: 754-755.
12 Smith, C., M. Reichard, P. Jurajda and M. Przybylski. 2004. The reproductive ecology of the European bitterling (Rhodeus sericeus). J. Zool., Lond., 262: 107-124.   DOI
13 NFRDI. 2007. Ecological survey and management strategy of introduced freshwater fishes. Report of national fisheries research and development institute (inland fisheries research institute). Gapyeong, Korea, 81pp. (in Korean)
14 Park, S.C., K.Y. Lee, T.B. Choi, W.M. Kim, M.J. Kim and J.S. Choi. 2013. The fish fauna and disturbance of geographical distributions in the eastern civilian control line of Korea. J. Environ. Impact Assess., 22: 27-37. (in Korean)   DOI
15 Slechtova, V., J. Bohlen and H.H. Tan. 2007. Families of Cobitidea (Teleostei: Cypriniformes) as revealed from nuclear genetic data and the position of the mysterious genera Barbucca, Psilorhynchus, Swepenticobitis and vaillantella. Mol. Evol., 44: 1358-1365.   DOI
16 Song, H.H. 1976. Study on the spawning and early development of Coreoleuciscus splendidus Mori. Master's thesis, Kyunghee University, 23pp. (in Korean)
17 Song, H.B. and O.K. Kwon. 1993. Ecology of Coreoleuciscus splendidus Mori (Cyprinidae) in Hongchon River. Korean J. Limnol., 3: 235-244. (in Korean)
18 Song, H.B. and G.M. Park. 2006. A molecular genetic variation among intra-populations of Korean shiner, Coreoleucuscus splendidus Mori (Cyprinidae)., Koran J. Ichthyol., 18: 78-86.
19 Song, H.B., M.S. Byeon, D.W. Kang, C.Y. Jang, J.S. Moon and H.K. Park. 2012. Population structure of bluegill, Lepomis macrochirus in lakes of the Han river system, Korea. Koran J. Ichthyol., 24: 278-286. (in Korean)
20 Song, H.Y. and I.C. Bang. 2009. Genetic variation of Coreoleuciscus splendidus populations from four major river in Korea as assessed by RAPD PCR. Korean J. Ichrhyol., 21: 129-133. (in Korean)
21 Candolin, U. and J.D. Reynolds. 2002. Why do males tolerate sneakers? Tests with the European bitterling, Rhodeus sericeus. Behav. Ecol. Sociobiol., 51: 146-152.   DOI
22 April, J., R.L. Mayden, R.H. Hanner and L. Bernatchez. 2011. Genetic calibration of species diversity among North america's freshwater fishes. Nat. Acad. Sci., 108: 10602-10607.   DOI
23 Asahida, T., T. Kobayashi, K. Saitoh and I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish. Sci., 62: 727-730.
24 Byeon, H.K. and J.K. Oh. 2015. Fluctuation of fish community and inhabiting status of introduced fish in Gangeumgnamdae stream, Korea. Korean J. Environ Ecol., 29: 718-728. (in Korean)   DOI
25 Cebrat, A., A. Cebula, A. Laszkiewicz, M. Kasztura, A. Miazek and P. Kisielow. 2008. Mechanism of lymphocyte-specific inactivation of RAG-2 intragenic promoter of NWC: Implications for epigenetic control of RAG locus. Mol. Immunol., 45: 2297-2306.   DOI
26 Chae, B.S. 2003. Introduction of native fish species to the other river system. Case study. In: The introduced fishes of Korea. The Ichthyological Society, pp. 65-86. (in Korean)
27 Chae, B.S., S.K. Kim, Y.H. Kang, N.S. Heo, J.M. Park, H.U. Ha and U.W. Hwang. 2015. Ichthyofauna and fish community structure in upper reach of the Nakdong River, Korea. Korean J. Ichthyol., 27: 116-132. (in Korean)
28 Chevassus, B. 1983. Hybridization in fish. Aquaculture, 33: 245-262.   DOI
29 Song, H.Y., K.Y. Kim, M.G. Yoon, Y.K. Nam, D.S. Kim and I.C. Bang. 2010. Genetic variation of Coreoleuciscus splendidus population (Teleostei; Cypriniformes) from four major river drainage system in South Korea as assessed by AFLP markers. Genes & Genomics, 32: 199-205.   DOI
30 Song, H.Y. and I.C. Bang. 2015. Coreoleuciscus aeruginos (Teleostei: Cypriniformes: Cyprinidae), a new species from the Seomjin and Nakdong rivers, Korea. Zootaxa, 3931: 140-150.   DOI
31 Sonnenberg, R., A.W. Nolte and D. Tautz. 2007. An evaluation of LSU rDNA D1-D2 sequences for their use in species identification. Frontiers in Zool., 4: 6.   DOI
32 Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nuclecis Acids Res., 24: 173-216.
33 Hauswith, W.W. and D.A. Clayton. 1985. Length heterogeneity of a conserved displacement-loop sequence in human mitochondrial DNA. Nucl. Acids Res., 13: 8093-8104.   DOI
34 Choi, C.G., Y.J. Hwang, M.S. and M.S. Na. 1995. A natural hybrid between spinous laoch, Cobitis longicorpus and cyprinid loach Misgurunus anguilicaudatus (Pices, Cobitis). Korean J. Ichthyol., 7: 203-207. (in Korean)
35 Epifanio, J. and D. Philipp. 2001. Simulating the extinction of parental lineages from introgressive hybridization: the effects of fitness, initial proportions of parental taxa, and mate choice. Rev. Fish Biol. Fisheries, 10: 339-354.
36 Hall, T.A. 1999. BioEdit: A user-friendly biogical sequence aligment editor and analysis program for windows 95/98/NT. Nucl. Acids. Symp. Set., 41: 95-98.
37 Jang, M.H., G.I. Cho and G.J. Joo. 2001. Fish fauna of the main channel in the Nakdong river. Korean J. Limnol., 34: 223-238. (in Korean)
38 Jansson, H., U. Holmgrea, K. Wedin and T. Andersson. 1991. High frequency of natural hybrids between trout, S. trutta L., in a Swedish river. J. Fish Biol., 39: 343-348.   DOI
39 Kanoh, Y. 2000. Reproductive success associated with territoriality, sneaking, and grouping in male rose bitterings, Rhodeus ocellatus (Pisces: Cyprinidae). Environ. Biol. Fishes, 57:143-154.   DOI
40 Kim, C.H., K.E. Hong, J.H. Kim and K.H. Kim. 2006a. Ichthyofauna in Yeongok stream, ganneung, Korea. Korean J. Ichthyol., 18: 244-250. (in Korean)
41 Kim, C.H., W.O. Lee, K.E. Hong, C.H. Lee and J.H. Kim. 2006b. Icthyofauna and fish community structure in Namdae stream, Yangyang, Korea. Koran J. Ichthyol., 18: 112-118. (in Korean)
42 Kim, K.S., H.R. Lee, A. Sade and I.C. Bang. 2014. Identification of hybrid between the tiger grouper Epinephelus fuscoguttatus and the giant grouper E. lanceolatus by analyzing COX I and RAG 2 sequences. Korean J. Ichthyol., 26: 70-73. (in Korean)
43 Kim, C.H., W.O. Lee, Y.J. Kang and J.M. Baek. 2010. Occurrence of a natural intergeneric hybrid, Rhodeus uyekiixAcheilognathus signifer (Pisces: Cyprinidae) from Jojongcheon Bukhan River, Korea. Korean J. Ichthyol., 22: 225-229. (in Korean)
44 Kim, H.S., S.W. Yun, J.G. Ko and J.Y. Park. 2014. Occurrence of a natural intergeneric hybrid between Rhodeus pseudosericeus and Acheilognathus signifer (Pisces: Cyprinidae) from the Namhangang (river), Korea. Koran J. Ichthyol., 26: 153-158.
45 Kim, I.S. 1997. Illustrated encycolopedia of fauna & flora of Korea, Vol. 37, Freshwater fishes. Ministry of Education, Yeongi, pp. 216-271. (in Korean)
46 Kim, I.S. and W.O. Lee. 1984. Effects of stream modification in the Seomjin river on the fish communities of the Dongjin river in Korea. Bull. Korean Fish. Soc., 17: 549-556. (in Korean)
47 Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.J. Kim and J.H. Kim. 2005. Illustrated book of Korean fishes. Kyohak Publishing Co. Ltd., Seoul, 615pp. (in Korean)
48 Kim, K.Y., M.H. Ko, S.J. Cho, W.J. Kin, M.H. Son and I.C. Bang. 2015. A natural hybrid of intergeneric mating between a female Puntungia herzi and a male Pseudorasbora parva (Cypriniformes: Cyprinidae). Fish. Aquat. Sci., 18: 99-107.
49 Kim, M.J. and C.B. Song. 2011. Origin of the Korean mandarin fish, Siniperca scherzeri and molecular phylogenetic relationships to other Siniperca fishes. Koran J. Ichthyol., 23: 95-105. (in Korean)