• Journal of Animal Science and Technology
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• 제50권2호
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• pp.177-184
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• 2008

Acid-labile subunit(ALS)는 85-kDa 크기의 당단백질로서 7.5-kDa의 insulin-like growth factor(IGF) 및 40~45-kDa IGF-binding protein-3와 결합하여 150-kDa ternary complex를 형성하는 혈장단백질이다. 선행연구에서 본 연구진은 reverse transcription-polymerase chain reaction(RT-PCR) 방법으로 돼지(porcine) ALS(pALS)의 coding sequence를 합성하여 plasmid vector에 삽입시켜 ‘expression construct’를 제작한 바 있다. 그러나 본 expression construct의 pALS coding sequence에는 PCR error로 추정되는 원인으로 말미암아 2개의 bases에서 mis-sense mutation이 일어난 것이 발견되었다. 본 연구에서는 ‘site-directed mutagenesis’ 방법으로 pALS의 올바른 coding sequence를 합성하여 ‘insert DNA’의 마지막 codon 다음에 ‘His-tag’ sequence가 위치한 pET- 28a(+) plasmid expression vector에 삽입하였다. 본 expression construct는 E. coli BL21(DE3) 세포에서 ‘induction’ 시켰고, 발현된 유전자재조합(recombinant) peptide는 Ni-affinity chromato- graphy로 정제하였다. 이렇게 affinity chro- matography로 정제된 peptide는 SDS-PAGE에서 66kDa 위치에 single band를 나타냄으로써 recombinant pALS의 예상된 질량과 일치하였다. 이상의 결과는 본 연구에서 recombinant pALS peptide가 성공적으로 발현정제되었음을 시사한다.

• 호남수학학술지
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• 제23권1호
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• pp.133-143
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• 2001

In this paper, we characterize some semi-invariant submanifolds of codimension 3 with almost contact metric structure (${\phi}$, ${\xi}$, g) satisfying 𝔏_ξ∇ = 0 in a nonflat complex space form, where ${\nabla}$ denotes the Riemannian connection induced on the submanifold, and 𝔏_ξ is the operator of the Lie derivative with respect to the structure vector field ${\xi}$.

• 대한수학회보
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• 제42권1호
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• pp.93-119
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• 2005

In this paper, we characterize some semi-invariant sub-manifolds of codimension 3 with almost contact metric structure ($\phi$, $\xi$, g) in a complex projective space $CP^{n+1}$ in terms of the structure tensor $\phi$, the Ricci tensor S and the Jacobi operator $R_\xi$ with respect to the structure vector $\xi$.

• 대한수학회보
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• 제36권3호
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• pp.513-532
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• 1999

The purpose of this paper is to give another new characterization of ruled real hypersurfaces in a complex space form $M_n$(c), c$\neq$0 in terms of the covariant derivative of its Weingarten map in the direction of the structure vector $\xi$.

• 대한수학회논문집
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• 제29권4호
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• pp.569-579
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• 2014

We consider a condition under which the projectivization $P(E^k)$ of a complex k-bundle $E^k{\rightarrow}M$ over an even-dimensional manifold M can have the hard Lefschetz property, affected by [10]. It depends strongly on the rank k of the bundle $E^k$. Our approach is purely algebraic by using rational Sullivan minimal models [5]. We will give some examples.

• 대한수학회보
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• 제57권4호
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• pp.873-890
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• 2020

In this paper, for discrete groups G and K, we show that the cohomology of the complex of projective tensor product B*(G)⨶B*(K) is isomorphic to the bounded cohomology Ĥ*(G × K) of G × K, which is the cohomology of B*(G × K) as topological vector spaces, where B*(G) is a complex of bounded cochains of G with real coefficients ℝ. In fact, we construct an isomorphism between these two cohomology groups that carries the canonical seminorm in Ĥ*(G × K) to the seminorm in the cohomology of B*(G)⨶B*(K).

• 대한수학회논문집
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• 제18권2호
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• pp.309-323
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• 2003

In this paper, We characterize a semi-invariant sub-manifold of codimension 3 satisfying ∇$\varepsilon$A = 0 in a complex projective space CP$\^$n+1/, where ∇$\varepsilon$A is the covariant derivative of the shape operator A in the direction of the distinguished normal with respect to the structure vector field $\varepsilon$.

• 대한수학회논문집
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• 제13권4호
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• pp.825-838
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• 1998

We prove that if a real hypersurface with constant mean curvature of a complex space form satisfying ▽$_{ξ/}$S = 0 and Sξ = $\sigma$ξ for a smooth function $\sigma$, then the structure vector field ξ is principal, where S denotes the Ricci tensor of the hypersurface.

• East Asian mathematical journal
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• 제37권1호
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• pp.97-107
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• 2021

Let M be a real hypersurface with almost contact metric structure (��, ξ, ��, g) in a nonflat complex space form Mn(c). We denote S and Rξ by the Ricci tensor of M and by the structure Jacobi operator with respect to the vector field ξ respectively. In this paper, we prove that M is a Hopf hypersurface of type (A) in Mn(c) if it satisfies Rξ�� = ��Rξ and at the same time satisfies $({\nabla}_{{\phi}{\nabla}_{\xi}{\xi}}R_{\xi}){\xi}=0$ or Rξ��S = S��Rξ.

• Kyungpook Mathematical Journal
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• 제61권1호
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• pp.181-190
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• 2021

Let M be a real hypersurface with almost contact metric structure (ϕ, ��, η, g) in a nonflat complex space form Mn(c). We denote S and R�� by the Ricci tensor of M and by the structure Jacobi operator with respect to the vector field �� respectively. In this paper, we prove that M is a Hopf hypersurface of type A in Mn(c) if it satisfies R��ϕ = ϕR�� and at the same time R��(Sϕ - ϕS) = 0.