• The Plant Pathology Journal
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• v.40 no.1
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• pp.40-47
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• 2024

Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.

• Mass Spectrometry Letters
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• v.15 no.1
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• pp.49-53
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• 2024

Ceramide is a lipid in which sphingoid bases and fatty acids are linked by amide bonds. As a marker of skin disease in the human stratum corneum, its disease-causing and therapeutic effects have been partially confirmed, and it is therefore an important element in commercially available cosmetic formulations. However, structural diversity caused by differences in the chain length, number, and location of hydroxyl groups makes quality control difficult. In this study, a method was established to separate different ceramide species using reversed-phase LC-MS/MS and thus enable qualitative evaluation. Separation of four standards was achieved within a short retention time, and the accuracy and sensitivity of the method were demonstrated by the low limit of detection (LOD) calculated based on the calibration curve showing linearity, with R² > 0.994. After verification of reproducibility and reliability through intra- and inter-day analyses, the efficiency of the method was confirmed through analysis of commercial cosmetic raw materials.

• Journal of the Korean Society for Marine Environment & Energy
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• v.14 no.4
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• pp.224-229
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• 2011

Recently, the management of the marine invasive alien species introduced by ballast water has come to the core as an urgent task. Because, hazardous organisms cause harmful effects on the ecosystem. Hazardous organisms also negatively affect fisheries, commercial shipping activities, and marine resources. In this regard, IMO used to operate GloBallast, Ballast Water Risk Assessment System, in order to manage the introduction of invasive species by ballast water. GloBallast is constructed to assess the relative risk due to the movement of ballast water between nations, using the volume and the number of times of ballast water discharge, the environmental similarity between ports, and the abundance of hazardous species as the main factors. According to the results of the demo-operation of GloBallast, it was found out that the environmental similarity factor had affected most highly the results of risk assessment. Therefore, in this study, the correlation between the main factors used in GloBallast risk assessment were re-examined, and some methods were suggested in order to reflect these main factors in the risk assessment more reasonable.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.53 no.1
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• pp.1-11
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• 2017

Fishing pots are one of the important fishing gears in our coastal and offshore fisheries. In terms of a sustainable use of marine resources, there are concerns about the ghost fishing by lost pots. It is reported that 10 to 70% of the pots are abandoned or lost annually. This study is to compare the catching efficiency of drum type whelk pots that are made of biodegradable nets to those made of the ordinary nets, in the eastern coast of Korea. Five types of drum pots were used with combination of biodegradable nets and ordinary (PA, PE) nets applied to the main and funnel parts of the pots: pots with biodegradable nets (Bio+Bio); pots with PE net (PE+PE); pots with body and funnel made of biodegradable nets and PE nets respectively (Bio+PE); pots with body and funnel made of PE nets and PA nets respectively (PE+PA); pots with body and funnel made of PE nets and biodegradable net respectively (PE+Bio). Field experiments were conducted from June $18^{th}$, 2015 to August $7^{th}$, 2015 using a commercial fishing vessel in the Uljin waters on the eastern coast of Korea. Two fleets of pots with one hundred in each, 20 pots of each type, were casted for 8 to 15 days to analyze their catching efficiency. The catch of target species, whelks, was 202,563.0 g with catch rate of 38.7% of the total catch, while the bycatch was 320,709.7 g with the rate of 61.3% of the total catch. The catch of whelks was the highest in the Bio+PE pots (46,020.3 g), followed by the Bio+Bio pots (42,027.5 g), the PE+Bio pots (41,849.9 g) and the lowest being PE+PE pots (38,054.2 g). Compared to the conventional pots, the pots with biodegradable nets on the body or entrance part had slightly higher catch rate for both the target species and the bycatch. The PE+PE had the lowest catch rate. There is no significant difference in catch between the pots with biodegradable nets and the ordinary nets for the target species such as Buccinum opisthoplectum, Neptunea eulimata, Buccinum striatissimum. Consequently, using biodegradable nets for the conventional whelk pots can reduce ghost fishing by lost gears while keeping the performance of the pots.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.3
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• pp.386-392
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• 1998

An objective index for quality estimation of anchovy sauce, our traditional seafood, has been required because inadequate standard causes dispute about quality estimation. Qualities of anchovy sauce made by traditional method and commercial anchor sauce products were compared by investigating their proximate compositions, total nitrogen contents, amino acid contents, development and level of a specific peptide on SDS-polyacrylamde gel electrophoresis (SDS-PAGE) and isoelectric point (pI) of the specific peptide. Also, pIs of IEF were attemped to identify fish species of salt-fermented sauces. The 55,600 and 46,900 dalton of peptide band were identified in all experimental samples. Especially, the area of 55,600 dalton was closely correlated with total nitrogen contents. Also, specific pIs in accordance with fish species were identified. The results suggest that correlation between area of 55,600 dalton and total nitrogen contents are used of index for quality estimation, and specific pIs are used of fish species identification.

• Research in Plant Disease
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• v.12 no.3
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• pp.272-277
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• 2006

Recycled irrigation water is a primary inoculum source of Phytophthora spp. and is capable of spreading propagules throughout nursery cultivation. Ozonation is commonly used to disinfest the recycled irrigation water; however, ozone has not been fully researched as a disinfectant for this purpose. In this study, zoospores of four species of Phytophthora were exposed for $1{\sim}9$ min to free available ozone at $0.1{\sim}0.3,\;0.5{\sim}0.7,\;0.9{\sim}1.2,\;1.4{\sim}1.7\;and\;1.9{\sim}2.2mg/l$. Zoospores, mycelial fragments, and culture plugs of P. nicotianae also were exposed to ozone concentrations ranging from 0.1 to 2.2 mg/l for periods ranging from 1 to 9 min. In addition, ozonated water was assayed monthly in 2004 and 2005 at two commercial nurseries, and quarterly in the first year at two other nurseries in Suwon, for ozone and survival of pythiaceous species using a selective medium. No zoospores of any species tested survived endpoint free ozone at 1.4 mg/l while limited mycelial fragments of P. nicotianae survived at 1.9 mg/l, and mycelial plugs treated at the same level of ozone were able to produce few sporangia. Phytophthora spp. were recovered only from nursery irrigation water with levels of free ozone at 0.3 mg/l or lower. The results of this study are essential for improving current ozonation sterilization.

• Journal of Forest and Environmental Science
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• v.11 no.1
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• pp.81-101
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• 1995

Random amplified polymorphic DNA (RAPD) technology, a recent approach in molecular genetics, is much usable to select the elite trees and to maximize the genetic gain in forest tree breeding program, providing a clue to determine the genetic marker-trait correlation. This review intorduces research on bark thickness and breeding strategy in Pinus elliottii, Pinus caribaea and their hybrid by Queensland Forest Service and ForBio Research Pty Ltd, University of Queensland, which employ RAPD technology. Genetic linkage map of $F_1$ hybrids includes 186 RAPD markers and 16 linkage groups (1641 cM long in total) and 6 quantitative trait loci are located putatively for bark thickness. Following recent research results and experiences in pine breeding programs, the forseeable stages in the application and development are proposed for marker assisted selectin; stage 1-determination of species specific markers for genes controlling traits of commercial interest, and stage 2-determination of marker-allele association for specific allelic variants within pure species. As pines inherit their megagametophytes from the seed parent and zygotic embryos from both male and female parents, the determination of marker-trait correlation is possible even in embryo stage, eventually making ways for the early selection of elite individuals.

• Journal of Mushroom
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• v.3 no.2
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• pp.52-59
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• 2005

The Aphyllophorales is a large order containing about 2,000 known species. Many of these are the bracket and coral fungi. The vast majority of these fungi are saprophytic on the plant debris. Many species are significant in decomposing plant remains, as they are able to digest cellulose or lignin that occurs in plant cell walls. Many of these fungi have been involved in everyday human affairs. A few were used medicinally by the Greeks and Romans as a remedy for many complaints, including colic, fractured limbs and bruises. Other bracket fungi have been used as curry combs for horses, as snuff, as razor strops and as a source of dye for clothing. The texture of the basidiocarp may be similar to that of cork, wood, leather, paper, or cartilage. Unlike the basidiocarps of the Order Agaricales, the basidiocarps of the Aphyllophorales are not fleshly and moist. Division of the members of the Aphyllophorales into genera was originally made on the basis of gross morphology of the basidiocarp and hymenium and Donk(1964) recognizes 22 families in this order. The species and genus whose typical in Aphylloporales were listed in Table. with related information. The ITS region sequence of some genus were found by BLAST search. Sequences retrieved from GenBank were visually aligned by the program CLUSTAL G. As a result, the medicinal mushroom was separated in four groups. In this multiple alignment, the sequence analysis among Fomes group, Inonotus group and Phellinus group showed high genetic similarity except Hericium group and Sparassis group.

• Journal of the Korean Wood Science and Technology
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• v.37 no.1
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• pp.65-72
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• 2009

Variation of wood rays within a growth ring and along axial direction in Pinus koraiensis and Larix kaempferi, which are important Korean commercial softwoods, was examined to get some useful information for their effective utilization. Ray height in cell number and ray number per $0.25mm^2$ in tangential section were investigated by optical microscopy. The average heights of uniseriate rays in Pinus koraiensis and Larix kaempferi were 6.3 and 9.1 cells, respectively. The average numbers of uniseriate rays per $0.25mm^2$ in Pinus koraiensis and Larix kaempferi were 6.5 and 7.5, respectively. The average heights of fusiform rays were about 20 cells but were not significantly different in both species. From earlywood to latewood within a growth ring, the heights of uniseriate and fusiform rays decreased but their numbers remained almost constant. Along axial direction, the heights of uniseriate rays in both species increased with the increase of stem height, and the numbers of uniseriate rays per $0.25mm^2$ in Pinus koraiensis were nearly constant from 0.2 m to 5.2 m but increased up to 19.2 m beyond 5.2 m. However, a constant pattern in the numbers of uniseriate rays was not observed in Larix kaempferi. The heights of fusiform rays in both species were the highest in middle part of stems but were the lowest in base and top of trees. In conclusion, the ray variation pattern was comparatively clear within a growth ring but not clear along axial direction.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.3
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• pp.188-198
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• 2005

This study was conducted to survey the catches of vertical bottom longline fisheries in the sea mount of central northern Pacific($30^{\circ}-42^{\circ}N$, $170^{\circ}-175^{\circ}E$), during the period of July 1 to August 25,2004 by commercial fishing vessel. The number of 57 test fishing was carried out in the central northern Pacific during 43 days and the total catches were 21,092.4kg as 19 fish species, CPUE/day and catches/day were 185 baskets and 490.5kg, respectively. Main fish species caught from the experimental fishing were Squalus mitsukurii (66.3%), Coelorhyrchus asperocephaius (11.7%) and Helicolenus avius (9.8%) and, average inside diameter for fish mouth was 4.0cm over. Catch ratio according to each fishing ground was the order of F, D, J, B and C. Catch ratio fur water depth was the order of 450-500m, 350-400m, 300-350m, 400-450m, 1000-1100m and 500-550m and, main species by water depth was Squalus mitsukurii for 300-400m, Etmopterus lucifer for 300-550m, Coelorhyrchus asperocephaius far 1,000m over. Catch ratio according to the kind of hooks was higher at the hook no.6 for Squalus mitsukurii and no. 5 for Etmopterus lucifer and, catch ratio by baits was higher at squid for Squalus mitsukurii, saury and eel for Helicolemus avius and saury for Etmopterus lucifer. Accordingly, it is thought that the extension of fishing hours is needed with the reduction of damage and loss for fishing gears during fishing operation.