• Journal of Sericultural and Entomological Science
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• v.51 no.1
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• pp.73-77
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• 2013

Silkworm transgenesis scientists have done some genetic modification work on multivoltine silkworms, but that type of silkworms is less commercial feasible. They are easy to manipulate, because they breed all year round. But the commercial silkworm variety must undergo hydrochloric acid treatment at a high temperature to be artificially hatched. Hydrochloric acid penetrates through the holes in the silkworm eggs, fatally damaging their reproduction. So it had been thought that altering the properties of the commercial silkworm variety would be very difficult. So we have tried to make from diapause to non-diapause eggs using diapauses varieties, 'Backokjam' and 'Jam 124'. At present, our group has establishing the conditions for non-diapause eggs. Oviposited eggs after 40 ~ 60 hours were incubated for 24 hours at $15{\sim}20^{\circ}C$ with dark condition. Non-diapause eggs were completely induced. The hatching rate, molting rate and pupation rate of non-diapause 'Jam 124' and 'Backokjam' eggs showed no differences compared to diapause eggs. When transgenic silkworm using the non-diapause eggs, the hatching rate showed that non-diapause eggs induced from diapause were 40 ~ 70%, diapause eggs treated with artificial incubation were 10 ~ 30%, and polyvoltine strains, HM eggs were 30 ~ 50%. Therefore, we suggest that modification techniques of the commercial silkworm eggs adequate for silkworm transgenesis can be used to develop transgenic silkworms more easily.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.772-781
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• 2013

The objective of this study was to develop simple sequence repeat (SSR) markers from expressed sequence tags (EST) of lettuce (Lactuca sativa) and identify 9 germplasms from 3 wild species of lettuce and 61 commercial cultivars using the developed EST-SSR markers. A total of 81,330 lettuce ESTs from NCBI databases were used to search for SSR and 4,229 SSR loci were identified. The highest proportion (59.12%, 2500) was represented by trinucleotide, followed by dinucleotide (29.70%, 1256) and hexanucleotide (6.62%, 280) among SSR repeat motifs. Totally 474 EST-SSR primers were developed from EST and a random set of 267 primers was used to assess the genetic diversity among 9 germplasms and 61 cultivars. Out of 267 primers, 47 EST-SSR markers showed polymorphism between 7 cultivars. Twenty-six EST-SSR markers among 47 EST-SSR markers showed high polymorphism, reproducibility, and band clearance. The relationship between 26 markers genotypes and 70 accessions was analyzed. Totally 127 polymorphic amplified fragments were obtained by 26 EST-SSR markers and two to nine SSR alleles were detected for each locus with an average of 4.88 alleles per locus. Average polymorphism information content was 0.542, ranging from 0.269 to 0.768. Genetic distance of clusters ranged from 0.05 to 0.94 between 70 accessions and dendrogram at a similarity of 0.34 gave 7 main clusters. Analysis of genetic diversity revealed by these 26 EST-SSR markers showed that the 9 germplasms and 61 commercial cultivars were discriminated by marker genotypes. These newly developed EST-SSR markers will be useful for cultivar identification and distinctness, uniformity and stability test of lettuce.

• Journal of Bio-Environment Control
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• v.11 no.3
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• pp.144-148
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• 2002

The overall objective of this study was to determine which variety of carrot-10 Japan varieties or 6 domestic varieties- produces the optimum quality, when cultivated on Jeju Island. Plant height, leaf number and leaf weight were observed to be more superior in the purebred variety ‘Jungang’than other varieties. further studies, however, skewed the crossing variety ‘Bureoun’was superior to all others. Furthermore, when comparing root diameter, root length and root weight, the ‘Bibary’was superior in both the purebred and crossing variety, When researching Japan varieties, the crossing variety, ‘Jung’showed an increase in underground root. The purebred variety ‘Donghae’also showed an increase in underground root. Marketable yields in domestic varieties were greatest in ‘Hapa’, while in Japan varieties, ‘Kaneko’was the most productive. The percentage of root cracked was 15％ greater in ‘Bureon’. In the purebred variety, Jungang, the percentage was only 10％. The percentage of roots branched was highest in ‘Hidomi’which showed a rate of 9%, The roots branched rate was 2% for ‘Jung’and 6％ for‘Donghae’. Considering the percentage of roots clacked or branched, the marketability of carrots was higher in Japan varieties in comparison with domestic varieties. Therefore we conclude that, in the cropping patterns of carrots, the domestic variety has an advantage over the Japan variety.

• Korean Journal of Computational Design and Engineering
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• v.7 no.4
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• pp.211-218
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• 2002

Swept volumes have been used in a wide variety of applications, and the literature contains much discussion of methods for computing the swept volumes in many situations. However, the commercially available CAD systems do not support the operations of generating the swept volumes enough to satisfy a variety of users' needs. In this paper, we present a new, simple and efficient algorithm for computing the swept volume of moving a polyhedron in 3-D region. The screw motion is used to describe the sweep motion of a polyhedron, because of its simplicity and computational advantages. The boundary of a swept volume is the result of combining the envelope surfaces and the partial boundaries at the initial and final position of a polyhedron. Some portions of these boundaries are inside the swept volume. We develop the algorithm to remove these interior portions. Then, to implement our algorithm, it is performed to integrate our program with the commercial CAD software, CATIA.

• Journal of Mushroom
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• v.10 no.3
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• pp.109-114
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• 2012

To develop new variety of oyster mushroom, 63 intra-specific hybrids between the strain Suhan and #Nongi201 were developed using hyphal anastomosis technique in 2004. The Po2008-275 hybrid between the dikaryon strain 04-154(Suhan x #Nongi201) and the monokaryon strain derived from ASI2487 were developed using hyphal anastomosis in 2008. The Po2008-275 was shown the best cultural characteristics, selected to be a new variety and named as 'Guseol'. The new commercial strain, 'Guseol' had dark grey pilei and grows well under spring and autumn conditions in Korea. The fruiting bodies of 'Guseol' were of an excellent quality in that not only the stipe was thick and long but also the pileus was small and hard. The optimum temperatures for mycelial growth and fruiting body development were $25{\sim}30^{\circ}C$ and $10{\sim}16^{\circ}C$, respectively. Time period required for the initiation of the first fruiting body was about 3 to 5 days depending on the temperatures. The shape of fruiting body was thin funnel shape. Fruiting body production per box($43{\times}43{\times}12cm$) was about $1545{\pm}400.9g$ which was almost 137% quantity compared to that of parental strain 04-154. Relatively low temperature incubation ($11^{\circ}C$) resulted in the development of better quality of 'Guseol' mushrooms. When two different media including potato dextrose medium and mushroom complete medium were compared, the mycelial growth of this mushroom were much faster in mushroom complete medium. Similar results were observed with other variety '#Chunchu2'. Analysis of the genetic characteristics of the new commercial strain 'Guseol' showed a major DNA profile as that of the parental 04-154 when primer URP 1, primer URP 2 and primer URP 5 were used, but different to '#Chunchu2' that was used as a control. This new variety of the dark grey oyster mushroom had smart and high quality image that corresponds well to "health food". We therefore expect that this new strain will satisfy the consumers demand for variety and excellent mushrooms.

• Horticultural Science & Technology
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• v.35 no.1
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• pp.142-146
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• 2017

In Korea, kiwifruit is grown within a limited region on the southern coast where the climate is warm. Since the yellow - fleshed kiwifruit variety, 'Hort16A', first became commercially available, we have focused on breeding additional yellow - fleshed kiwifruits. Here, we describe the cultivar 'Goldone', which originated from 'Red Princess' and is characterized by red coloration around the fruit core. Conventional field crosses were performed in 2003, and permission for final release of 'Goldone' was obtained in 2011. This cultivar is very productive, with an average fruit weight of 129 g, which is 39% heavier than that of the cultivar 'Hayward'. 'Goldone' is harvested in late October, approximately 165 - 170 days after anthesis. In general, 'Goldone' has approximately eight flowers per fruiting shoot; these flowers must be thinned before blooming for commercial production. 'Goldone' was registered at the Korean Seed & Variety Service in 2014 for plant variety protection rights (grant no. 4835).

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.10a
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• pp.584-590
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• 2009

Since driving include compilcated actions that require a variety of abillity and cause extrme concentration or strain, divers tend to feel tired easiy. However, divers can't recognize fatigue degree by himself and accordingly the methods to measure quantitative fatigue degree exactly is quite difficult to be secured. In this study, the most efficient driving posture was suggested based on the analysis of quantitative muscular strength and fatigue degree according to posture. The driver whom we experimented included 9 commercial bus drivers. We also analyzed quantitative legs' muscular strength according te operating each pedal, left and right then we analyzed muscular strength and muscular fatigue degree according to driving pattern while driving commercial buses. And then we suggested the most efficient driving posture.

• New & Renewable Energy
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• v.6 no.2
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• pp.5-11
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• 2010

This paper reviews three commercial softwares for wind climate data analysis in wind resource assessment; WAsP/Observed Wind Climate, WindRose and Windographer. Windographer is evaluated as the best software because of its variety of input data format, analysis functions, easiness of user interface, etc. For a quantitative understanding of uncertainty depending on software selection, a benchmark is carried out with the met-mast observation dataset at the Gimnyeong Wind Turbine Performance Test Site. It is found that Weibull parameter calculation and air density correction have a dependency on the software so that such uncertainty should be considered when an analysis software is selected. It is confirmed that annual energy production calculated by WAsP using a statistical table of frequency of occurrence may have some error compared to a time-series calculation method used by the other softwares.

• Korean Institute of Interior Design Journal
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• no.38
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• pp.83-90
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• 2003

Contemporary culture of consumption has been transferred from physical satisfaction in the past to the "pleasure" culture of consumption that satisfies emotional needs, generating a new design category - emotional design. The current study analyzes various environmental design techniques of the theme parks, which recognized early the importance of the "pleasure" culture of consumption and used the techniques for sales and as profit-making tools. This study also examines a trend of emotional design in environmental design techniques of the theme park and proves that the design techniques can be applied to a variety of interior design areas, as well as commercial facilities.mmercial facilities.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.32-38
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• 1999

A biosurfactant produced by Tsukamurella sp. 26A was purified by procedures including acid precipitation, ethylacetate extraction, and adsorption chromatography. The purified biosurfactant reduced the surface tension of water from 72 mN/m to 30 mN/m at a concentration of 250 mg/l, whereas the minimum interfacial tension against n-hexadecane was lowered to 1.5 mN/m at a concentration of 40 mg/i. The compound stabilized oil-in-water emulsions with a variety of commercial oils and had strong emulsification and stabilization activities when compared to those of commercial emulsifiers and stabilizers. Surface tension was stable over a broad range of pH (2-12) and temperature ($100^{\circ}C$, 3h). The biosurfactant was identified as glycolipid having a hydrophilic moiety of trehalose.