• BMB Reports
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• v.53 no.11
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• pp.576-581
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• 2020

Dimethylation of the histone H3 protein at lysine residue 9 (H3K9) is mediated by euchromatin histone methyltransferase II (EHMT2) and results in transcriptional repression of target genes. Recently, chemical inhibition of EHMT2 was shown to induce various physiological outcomes, including endoplasmic reticulum stress-associated genes transcription in cancer cells. To identify genes that are transcriptionally repressed by EHMT2 during apoptosis, and cell stress responses, we screened genes that are upregulated by BIX-01294, a chemical inhibitor of EHMT2. RNA sequencing analyses revealed 77 genes that were upregulated by BIX-01294 in all four hepatic cell carcinoma (HCC) cell lines. These included genes that have been implicated in apoptosis, the unfolded protein response (UPR), and others. Among these genes, the one encoding the stress-response protein Ras-related GTPase C (RRAGC) was upregulated in all BIX-01294-treated HCC cell lines. We confirmed the regulatory roles of EHMT2 in RRAGC expression in HCC cell lines using proteomic analyses, chromatin immune precipitation (ChIP) assay, and small guide RNA-mediated loss-of-function experiments. Upregulation of RRAGC was limited by the reactive oxygen species (ROS) scavenger N-acetyl cysteine (NAC), suggesting that ROS are involved in EHMT2-mediated transcriptional regulation of stress-response genes in HCC cells. Finally, combined treatment of cells with BIX-01294 and 5-Aza-cytidine induced greater upregulation of RRAGC protein expression. These findings suggest that EHMT2 suppresses expression of the RRAGC gene in a ROS-dependent manner and imply that EHMT2 is a key regulator of stress-responsive gene expression in liver cancer cells.

• Archives of Reconstructive Microsurgery
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• v.19 no.1
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• pp.50-55
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• 2010

Injury of the musculocutanous nerve can be associated with a proximal humeral fracture or shoulder dislocation, and injury of the brachial plexus. However, injury of this nerve associated with a humeral shaft fracture has rarely been reported. Diagnosis of the musculocutaneous nerve injury is difficult because its sensory loss is ill-defined, and examination of elbow flexion is difficult when it is associated with fractures. We report an unusual case of musculocutaneous nerve injury in a 27 years old woman who had multiple injuries including a humerus shaft fracture, an ipsilateral radius shaft fracture, and an associated radial nerve laceration. Diagnosis of the musculocutaneous nerve injury was delayed because combined fractures of the humerus and radius prevented proper examination of the elbow motion and nerve grafting of the radial nerve delayed early elbow motion exercise. Delayed exploration of the musculocutaneous nerve 6 months after trauma showed complete rupture of the nerve at its entry into the coracobrachialis muscle and the defect was successfully managed by sural nerve graft.

• The Journal of the Acoustical Society of Korea
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• v.15 no.5
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• pp.44-52
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• 1996

Acoustical characteristics of internal pipe structures and a loudspeaker of the thermoacoustic refrigerator are analyzed by using the transfer matrix method. The resonator system is dismantled into verious basic acoustic elements, and then linearized transfer matrices are serially combined with the dynamical system of linearized loudspeaker model, that the total system of thermoacoustic refrigerator can be analyzed in terms of frequency characteristics and acoustic field shape. Additionally, by using equations for energy flow through the capillary stack, the temperature distribution over the stack is numerically estimated. After expressing the acoustic work flow, thermoacoustic flow, and energy loss per unit length in a single capillary duct by using the transverse functional variations, overall energy flow rate and energy balance are obtained for the whole capillary stack. The final expression for energy flow through the stack is numerically evaluated by varying physical parameters obtained from the sound field analysis. After confirming good agreements between predicted and experimental results for the interior sound field of a refrigerator model, the thermoacoustic characteristics of Hofler's apparatus is analyzed by the proposed method and it is observed that the results agree well with Hofler's experimental results.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.30 no.1
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• pp.17-22
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• 2017

In this study, we designed the metallic reception part of a patch antenna using the phase field design method. The design object function is formulated with the S-parameter value which represent the return loss so that it is targeted to maximize radiation efficiency at a target frequency. The initial model of a patch antenna was designed via the ordinary theory based approach and its performance was enhanced by changing the structural configuration of the metallic part using the phase field design method combined with the double well potential functions. The final shape was proposed by removing the gray scale area along the structural boundary by employing a cut-off method. The proposed shape shows that the radiation efficiency at target frequency is significantly improved compared with the initial patch shape. The finite element analysis and optimization precess was performed using the commercial package COMSOL and Matlab programming.

• Journal of Korean Medicine for Obesity Research
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• v.7 no.1
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• pp.39-54
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• 2007

Objectives : This study has been done to know which herbs and herbal formulae are frequently used for the treatment of obesity in Korea and overseas since 2000. Methods : We conducted a computerized literature search in KISS (Korean studies Information Service System), Pubmed, Journal of Oriental Association for Study of Obesity ,and Journal of Oriental Rehabilitation Medicine(2000 -Apr. 2006) using search key words obesity, obese, weight loss, weight control combined with herb, herbal formula. Result and conclusion : Seventy-two studies have been identified(50 Korean papers and 22 pubmed papers) and 81 herbs have been found. In USA it was mainly used as single herb for food, but in Korea it was mainly used as herbal formula. For single herb, ephedra(麻黃) was the most studied single herb both in Korea and the USA. For herbal formulae, Sasang Constitutional Medicine(SCM, 19times) was the most frequently used herbal formula, in which the herbs for Tae-eum type person like Taeyeumjowui-tang(太陰調胃湯, 7times) and Choweseungcheng-tang(調胃升淸湯, 5times) were mainly prescribed. The next was Chegameuiin-tang(體減薏苡仁湯, 11times) and Bangpungtongsungsan( 防風通聖散, 3times). Calculating each herb consisted in each herbal formula, Coicis Semen(薏苡仁), Angelicae gigantis Radix(當歸), and Glycyrrhizae Radix(甘草), Raspberry Ketone, Cnidii Rhizoma(川芎), and Ephedra Herba(麻黃) turned out to be the most frequently used herb.

• Food Science and Preservation
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• v.8 no.3
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• pp.274-278
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• 2001

The antimicrobial extracts of Rheum palmatum and Coptis chinensis root as well as grapefruit seed extract were applied to dipping treatment for keeping qualities of zucchinis, respectively, which were then packed in low density polyethylene films incorporated with 1% antimicrobial extracts and stored at 10$\^{C}$. Dipping and packaging in the antimicrobial agents suppressed the growth of putrefactive microorganisms and the decay ratio of zucchinis. In addition, the loss ratio of ascorbic acid content of zucchinis and their weight were decreased during the storage of zucchinis. Consequently, the combined method of dipping and packaging in antimicmbial agents tumid out to be superior to dipping treatment or film-packaging in the view point of decay ratio and the quality control of zucchinis.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.319-319
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• 2017

Methane is the main greenhouse gas released from rice paddy field. Methane from paddy fields accounts for 11 % of the global total methane emission. The global warming potential (GWP) of methane is 25 times more than that of carbon dioxide on a mass basis. It is well known that most effective practice to mitigate methane in paddy is related to the water management during rice growing season and the use of organic matters. This study was conducted to investigate the effects of tillage and cultivation method on methane emission in paddy. Tillage (tillage and no-tillage) and cultivation methods (transplanting and direct seeding) were combined tillage-transplanting (T-T), tillage-wet hill seeding (T-W), tillage-dry seeding (T-D) and no-till dry seeding (NT-D) to evaluate methane mitigation efficiency. Daily methane emission was decreased on seeding treatments (T-W, T-D, NT-D) than transplanting treatment (T-T). Amount of methane emission during rice growing season is highest in T-T ($411.7CH_4\;kg\;ha^{-1}y^{-1}$) and lowest in NT-D treatment (89.7). In T-W and T-D treatments, methane emissions were significantly decreased by 36 and 51 % respectively compared with T-T. Methane emissions were highly correlated with the dry weight of whole rice plant ($R^2=0.62{\sim}0.93$). T-T treatment showed highest $R^2$ (0.93) among the four treatments. Rice grain yields did not significantly differ with the tillage and cultivation methods used. These results suggest that direct seeding practice in rice production could mitigate the methane emissions without loss in grain yield.

• Korean Journal of Veterinary Service
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• v.30 no.1
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• pp.175-181
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• 2007

Parasites cause great economic loss in livestock in Korea, and can be categorized as either or indirect losses. In order to monitor the parasites from October 2005 to January 2006, 401 samples were randomly collected from cattle farms and examined the prevalence of gastro-intestinal (GI) parasites by using the sucrose flotation method and formalin-ether centrifugation method. The overall infection rate of GI parasites was about 43.4%. Of these parasites, nematodes were identified as Capillaria bovis (5.5%), Strongyloides papillosus (2.5%), Trichuris discolor (1.2%). Cestoda was observed only one species, Moniezia benedeni (2.2%). The rate of Eimeria spp was 39.2%. A total of five species of Eimeria was identified; among the species identified as above E bovis and E zuernii appeared with relatively higher infection rates. The combined infection of parasites was 35.2% in single species, 29.0% in double and 2.0% in triple infections. No cases of clinical symptoms were observed in this survey. The EPG levels of each species of parasites were very low in all infected cases.

• Food Science of Animal Resources
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• v.31 no.3
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• pp.339-348
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• 2011

Ground pork containing 0, 1, 2, or 3% rice bran fiber was prepared. pH increased as the amount of rice bran fiber added increased (p<0.05) but decreased during storage. The lightness and redness values of the raw ground pork decreased with the addition of rice bran fiber, and ground pork containing 3% rice bran fiber had the highest yellowness value during early storage (p<0.05). Moreover, adding rice bran fiber was not associated with color stability during storage. Samples containing 2 or 3% rice bran fiber had improved cooking loss, hardness, gumminess, and chewiness. Furthermore, rice bran fiber enhanced tenderness, juiciness, and overall acceptability on a sensory evaluation. These physico-chemical properties were maintained to the final storage period. The best results were obtained with ground pork containing 2 or 3% rice bran fiber. Rice bran fiber combined with useful antioxidants may be a more effective approach to increase the stability of ground pork during cold storage.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.63 no.12
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• pp.1655-1660
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• 2014

In this paper, we propose a design of a triple-band microstrip circular patch antenna. The proposed antenna generates the triple frequency resonance at 1.85GHz(LTE), 2.45GHz(ISM) and 5.5GHz(WLAN). Firstly, we design the dual-band antenna. The dual-band antenna consist of the circular patch, slits, and the slot. The circular patch and slot are designed for dual frequency of 2.45GHz and 5.5GHz, respectively. And then the dual-band antenna is combined with additional arm-shaped structure for the triple-band characteristic. The arm-shaped structure is operated as the dipole. It is designed for lowest frequency of 1.85GHz. Each part of the antenna unites to a new structure. In order to design the proposed antenna automatically and optimally, APSO algorithm is adopted. During APSO, the mismatch of the proposed antenna is resolved. The optimal designed antenna has an acceptable return loss(-10dB) at each bands(i.e, 1.85GHz, 2.45GHz and 5.5GHz).