• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.1022-1030
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• 2004

The gene encoding Aquifex pyrophilus (Apy) DNA polymerase was cloned and sequenced. The Apy DNA polymerase gene consists of 1,725 bp coding for a protein with 574 amino acid residues. The deduced amino acid sequence of Apy DNA. polymerase showed a high sequence homology to Escherichia coli DNA polymerase I-like DNA polymerases. It was deduced by amino acid sequence alignment that Apy DNA polymerase, like the Klenow fragment, has only the two domains, the $3'{\rightarrow}5'$ exonuclease domain and the $5'{\rightarrow}3'$ polymerase domain, containing the characteristic motifs. The Apy DNA polymerase gene was expressed under the control of T7lac promoter on the expression vector pET-22b(+) in E. coli. The expressed enzyme was purified by heat treatment, and Cibacron blue 3GA and $UNO^{TM}$ Q column chromatographies. The optimum pH of the purified enzyme was 7.5, and the optimal concentrations of KCl and $Mg^{2+}$ were 20 mM and 3 mM, respectively. Apy DNA polymerase contained a double strand-dependent $3'{\rightarrow}5'$ proofreading exonuclease activity, but lacked any detectable $5'{\rightarrow}3'$ exonuclease activity, which is consistent with its amino acid sequence. The somewhat lower thermostability of Apy DNA polymerase than the growth temperature of A. pyrophilus was analyzed by the comparison of amino acid composition and pressure effect.

• Microbiology and Biotechnology Letters
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• v.31 no.3
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• pp.230-234
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• 2003

The Bacillus subtilis YvaE protein, the small multidrug resistance (SMR) family (TC #2.A. 7.1), is shown to catalyze efflux of multiple cationic drugs including many disinfectants, when it was cloned and expressed in Escherichia coli. When the yvaD gene was coexpressed with yvaE gene, the yvaD protein, encoded within a single operon with the yvaE gene, is shown to counteract the action ofYvaE. By ethidium efflux analysis, the cells harvoring a vector with yvaE gene showed a rapid ethidium efflux, compared with the control cells. These results clearly suggest that YvaE mediates drug export from the cell cytoplasm.

• Microbiology and Biotechnology Letters
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• v.31 no.3
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• pp.307-310
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• 2003

A gene encoding streptodornase(sdc) from Streptococcus equisimilis H46A was expressed in S. equisimilis H64H sdc under the control of the streptokinase gene promoter. Secretion of the streptodornase was directed by the signal sequences of streptokinase or streptodornase. The expressed streptodornase activity from S. equisimilis H46A sdc transformant with streptokinase promoter - streptodornase coding sequence fusion vector was 2.3 fold higher than that from wild type. Construct of signal sequence region replaced by streptokinase ones was similarly expressed as a wild type. But constructs of skc or lrp core regions of streptokinase promoter streptodornase fusion were similarly expressed as in sdc mutant. In conclusion, improved expression of streptodornase by use of streptokinase promoter required the full length of promoter.

• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.110-118
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• 2003

Pseudomonas syringae pv. actinidiae strains, which cause canker disease in kiwifruit, were collected from kiwifruit orchards in Korea and identified using biochemical and physiological tests. The nucleotide sequences of the 16s rDNA and 16s-23s internally transcribed spacer of the isolates were found to be Identical to those of' the pathotype strain, Kwl 1, of P syringae pv. actinidiae. Remarkably, no coding sequence for phaseolotoxin biosynthesis or phaseolotoxin- resistant ornithine carbamoyltransferase was found by PCR amplification in any of the new Korean isolates of pseudomonas syringae pv. actinidiae, although this was clearly identified in the control pathotype Kwl 1 reference strain. In contrast, three primer sets derived from the coronatine biosynthetic gene cluster and DNA from the Korean strains yielded amplified DNA fragments of the expected size. A sequence analysis of the PCR products revealed that P. syringae pv. actinidiae and the Korean strains of pv. actinidiae contain coronafncate ligase genes (cfl)with identical sequences, whereas their. corR genes exhibited 91% sequence similarity. The production of coronatine, instead of phaseolotoxin, by the Korean strains of P. syringae pv. actinidiae was confirmed by a bioassay using reference pathovars known to produce coronatine and phaseolotoxin. The genes for coronatine biosynthesis in the Korean strains of P. syringae pv. actinidiae were found to be present on plasmids.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.1230-1242
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• 1993

In a case of mushroom (Lentinus Edodes L.) , visual features are crucial for grading and the quantitative evaluation of the growth state. The extracted quantitative visual features can be used as a performance index for the drying process control or used for the automatic sorting and grading task. First, primary external features of the front and back sides of mushroom were analyzed. And computer vision based algorithm were developed for the extraction and measurement of those features. An automatic thresholding algorithm , which is the combined type of the window extension and maximum depth finding was developed. Freeman's chain coding was modified by gradually expanding the mask size from 3X3 to 9X9 to preserve the boundary connectivity. According to the side of mushroom determined from the automatic recognition algorithm size thickness, overall shape, and skin texture such as pattern, color (lightness) ,membrane state, and crack were quantified and measured. A portion of t e stalk was also identified and automatically removed , while reconstructing a new boundary using the Overhauser curve formulation . Algorithms applied and developed were coded using MS_C language Ver, 6.0, PC VISION Plus library functions, and VGA graphic function as a menu driven way.

• Journal of Microbiology and Biotechnology
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• v.9 no.2
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• pp.230-233
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• 1999

A plasmid vector was constructed for the expression and secretion of Bacillus macerans cyclodextrin glucanotransferase (CGTase) in Bacillus subtilis. The vector, pUBACGT, was composed of the ribosome-binding sequence, signal sequence, and cgt gene from B. macerans under the control of amyR2, the promoter of amyE gene coding for $\alpha$-amylase from B. subtilis var. natto. Bacillus subtilis LKS88, a mutant strain lacking genes for an amylase and two proteases, was used as a host for the transformation of the plasmid vector. The transformants were selected on kanamycin-containing Luria-Bertani plates. The starch hydrolyzing activity was observed on the starch-containing plates by the iodine method and cyclodextrin-forming activity was detected in the culture medium. A SDS-PAGE analysis showed that most of the expressed CGTase in the recombinant B. subtilis was secreted into the medium at a high expression level.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.2A
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• pp.168-175
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• 2006

To cope with channel variation, wireless networks such as IEEE 802.11 WLAN provide multiple transmission rates by employing different channel modulation and coding schemes. However, the coexistence of different transmission rates degrades the total system performance of the network. In order to eliminate this performance abnormality and improve protocol capacity, we propose a new Packet transmission algorithm, the RAT(Rate-Adapted Transmission) scheme. The RAT scheme distributes the wireless channel fairly based on the channel occupancy time. Moreover, it efficiently transmits packets even in a single station using rate-based queue management. Therefore, the RAT scheme obtains not only the inter-rate contention gain among stations but also the intra-rate contention gain among connections in a single station.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.2485-2489
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• 2005

This paper introduces a new preprocessing scheme to replace original data of gray scale images with particular ordered data so that performance of lossless compression can be improved more efficiently. As a kind of preprocessing technique to maximize performance of entropy encoder, the proposed method converts the input image data into more compressible form. Before encoding a stream of the input image, the proposed preprocessor counts co-occurrence frequencies for neighboring pixel pairs. Then, it replaces each pair of adjacent gray values with particular ordered numbers based on the investigated co-occurrence frequencies. When compressing ordered image using entropy encoder, we can expect to raise compression rate more highly because of enhanced statistical feature of the input image. In this paper, we show that lossless compression rate increased by up to 37.85% when comparing results from compressing preprocessed and non-preprocessed image data using entropy encoder such as Huffman, Arithmetic encoder.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2014.05a
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• pp.52-53
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• 2014

The Work Breakdown Structure (WBS) is a primary tool which provides a framework that defines clear scope of all deliverables throughout the project life cycle. Once the WBS is established in projects, it should allow project team members to measure and manage work performances by the WBS; further, it should provide a reference point when any work scope needs to be redefined. Based on the project information in the Progress and Performance Measurement System (PPMS) of UAE's Barakha Nuclear Power Plant (BNPP) projects, an attempt was made to develop a new WBS which provides hierarchical and systematical decomposition of the total work scope of NPP construction projects while avoiding from the preexistence concept in Korean NPP projects that the WBS is a combination of Physical Breakdown Structure (PBS) and Functional Breakdown Structure (FBS). The unique features of the new WBS are as follows: (1) defined the definition of each level of the WBS, (2) subdivided the WBS into 5 hierarchical levels, and (3) adopted globally used general coding structure. The new WBS provides a basic hierarchical structure for the project scope and can be used as a basic tool for schedule control, performance measurement, project status monitoring, and communication among project participants. In addition, by putting the Work Package (WP) under the WBS, the Earned Value Management System (EVMS) per WP can be utilized for the project.

• Speech Sciences
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• v.10 no.2
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• pp.99-109
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• 2003

The purpose of this study was to analyze and compare head register with chest register of singers acoustically. Fifteen healthy tenor major students were participated. Fifteen healthy untrained adults were chosen as the control group for this study. Long term average (LTA) power spectrum using the Fast Fourier transform (FFT) algorithm and Linear predictive coding (LPC) filter response were made with /a/ sustained in both head (G4, 392 Hz) and chest registers (C3, 131 Hz). Statistical analysis was performed using the Mann-Whitney test. In the LTA power spectrum, head register of singers increased in the level of energy gain within the frequency of 2.2-3.4 kHz (p<0.01), and 7.5-8.4 kHz (p<0.01, p<0.05). Chest register of singers increased in the frequency of 2.2-3.1 kHz (p<0.01), 7.8-8.4 kHz (p<0.05) and around 9.6 kHz (p<0.01). The LTA power spectrum revealed a peak of acoustic energy around 2,500 Hz, known as the singer's formant and another peak of acoustic energy around 8,000 Hz in the singer's voice.