Browse > Article

A Drug Efflux Pump for Cationic Drugs including Disinfectants in Bacillus subtilis  

Yong Joon Chung (School of Natural Science, Jeonju University)
Publication Information
Microbiology and Biotechnology Letters / v.31, no.3, 2003 , pp. 230-234 More about this Journal
Abstract
The Bacillus subtilis YvaE protein, the small multidrug resistance (SMR) family (TC #2.A. 7.1), is shown to catalyze efflux of multiple cationic drugs including many disinfectants, when it was cloned and expressed in Escherichia coli. When the yvaD gene was coexpressed with yvaE gene, the yvaD protein, encoded within a single operon with the yvaE gene, is shown to counteract the action ofYvaE. By ethidium efflux analysis, the cells harvoring a vector with yvaE gene showed a rapid ethidium efflux, compared with the control cells. These results clearly suggest that YvaE mediates drug export from the cell cytoplasm.
Keywords
Efflux pump; cationic drugs; Bacillus subtilis;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Prevention of drug access to bacterial targets: Permeability barries and active efflux /
[ Nikaido,H. ] / Science   DOI
2 Tight regulation, modulation, and high-level expression vectors containing the arabinose pBAD promoter /
[ Guzman,L.M.;D.Belin;M.J.Carson;J.Beckwith ] / J. Bacteriol.
3 A functional/phylgenetic classification system for transmembrane solute transportes /
[ Saier Jr,M.H. ] / Microbiol. Mol. Biol. Rev.   DOI   ScienceOn
4 EmrE, an Escherichia coli 12-kDa multidrug transporter, exchanges toxic cations and <TEX>$H^+$</TEX> and is solube in organic solvents /
[ Yerushalmi, H.;M.Lebendiker;S.Schudiner ] / J. Biol. Chem.   DOI   ScienceOn
5 An essential glutamyl residue in EmrE, a multidrug antiporter from Escherichia coli /
[ Yerushalmi,H.;S.Schuldiner ] / J. Biol. Chem.   DOI   ScienceOn
6 A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis /
[ Masaoka,Y.;Y.Ueno;Y.Morita;T.Kuroda;T.Mizushima;T.Tsuchiya ] / J. Bacteriol.   DOI   ScienceOn
7 Bacterial multidrug resistance is due to a single membrane protein which functions as a drug pump /
[ Grinius,L.L.;E.B.Goldberg ] / J. Biol. Chem.
8 Microbial genome analysis; comparative transport capabilites in eighteen prokaryotes /
[ Paulsen,I.T.;L.Nguyen;M.K.Sliwinski;R.Rabus;M.H.Saier Jr ] / J. Mol. Biol.   DOI   ScienceOn
9 Molecular characterization of the staphylococcal multidrug resistance export protein QacC /
[ Paulsen,I.T.;M.H.Brown;S.J.Dunstan;R.A.Skurray ] / J. Bacteriol.
10 Scanning cysteine acessibility of EmrE, and <TEX>$H^+$</TEX> coupled multidrug transporter from Escherichia coli,reveals a hydrophobic pathway for solutes /
[ Mordoch,S.S.;D.Granot;M.Lebendiker;S.Schuldiner ] / J. Biol. Chem.   DOI
11 Microbial genome analysis: Global comparisons for transport capabilities based on phylogenies, bioenergetics and substrate specificities /
[ Paulsen,I.T.;M.K.Sliwinski;M.H.Saier Jr ] / J. Mol. Biol.   DOI   ScienceOn
12 Protondependent multidrug efflux systems /
[ Paulsen,I.T.;M.H.Brown;R.A.Skurray ] / Microbiol. Rev.
13 Multidrug resistance pumps provide broad defense /
[ Lewis,K.;D.C.Hooper;M.Ouellette ] / ASM News
14 Negative dominance studies demonstrate the oligomeric structure of EmrE, a multidrug antiporter from Escherichia coli /
[ Yerushalmi,H.;M.Lebendiker;S.Schuldiner ] / J. Biol. Chem.   DOI   ScienceOn
15 A membrane-enbedded glutamate in required for ligand binding to the multidrug transporter EmrE /
[ Muth,T.R.;S.Schuldiner ] / EMBO J.   DOI   ScienceOn
16 The CLUSTAL X windows interface: flexible strategies for multiple alignment aided by quality analysis tools /
[ Tompson,J.D.;T.J.Gibson;F.Plewniak;F.Jeanmougin;D.G.Higgins ] / Nucleic Acids Res.   DOI   ScienceOn
17 Proteon motive force-driven and ATP-dependent drug extrusion systems in multidrug-resistant Lactococcus lactis /
[ Bolhuis,H.;D.Molenaar;G.Poelarends;H.W.van Veen;B.Poolman;A.J.M.Driessen;W.N.Konings ] / J. Bacteriol.
18 TMPred-a database of membrane spanning protei segments /
[ Hofmann,K.;W.Stoffel ] / Biol. Chem.
19 A broad-specificity multidrug efflux pump requiring a pair of homologous SMR-type proteins /
[ Jack,D.L.;M.L.Storms;J.H.Tchieu;I.T.Paulsen;M.H.Saier Jr ] / J. Bacteriol.   DOI   ScienceOn