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Expression of Streptodornase by Use of Streptokinase Promoter in Streptococcus equismilis H46A.  

Son, Hyun-Jung (Department of Biological Sciences, Chonnam National University)
Jin, Jong-Eon (Department of Cosmetology, Dongkang College)
Kim, Il-Chul (Department of Biological Science, KAIST)
Bae, Seok (Department of Biological Sciences, Chonnam National University)
Lee, Hwang-Hee (Department of Biological Sciences, Chonnam National University)
Publication Information
Microbiology and Biotechnology Letters / v.31, no.3, 2003 , pp. 307-310 More about this Journal
Abstract
A gene encoding streptodornase(sdc) from Streptococcus equisimilis H46A was expressed in S. equisimilis H64H sdc under the control of the streptokinase gene promoter. Secretion of the streptodornase was directed by the signal sequences of streptokinase or streptodornase. The expressed streptodornase activity from S. equisimilis H46A sdc transformant with streptokinase promoter - streptodornase coding sequence fusion vector was 2.3 fold higher than that from wild type. Construct of signal sequence region replaced by streptokinase ones was similarly expressed as a wild type. But constructs of skc or lrp core regions of streptokinase promoter streptodornase fusion were similarly expressed as in sdc mutant. In conclusion, improved expression of streptodornase by use of streptokinase promoter required the full length of promoter.
Keywords
Streptodornase; streptokinase; Streptococcus equisimilis H46A; streptokinase promoter;
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1 Colorimetric determination of DNase I activity with a DNA-methyl green substrate /
[ Sinicropi,D.;D.L.Baker;W.S.Prince;K.Shiffer;S.Shak ] / Anal. Biochem.   DOI   ScienceOn
2 Complex transcriptional control of the streptokinase gene of Streptococcus equisimilis H46A /
[ Gase,K.;T.Ellinger;H.Malke ] / Mol. Gen. Genet.   DOI   ScienceOn
3 Mutants of Diplococcus pnumoniae that lack deoxyribonuclase and other activities possibly pertinent to genetic transformation /
[ Lacks,S, ] / J. of Bacteriol.
4 Streptokinase:cloning,expression, and excretion by Escherichia coli /
[ Malke,H.;J.J.Ferretti ] / Proc. Natl. Acad. Sci. U.S.A.   DOI   ScienceOn
5 Isolation,sequence and expression in Escherichia coli,Bacillus subtilis and Lactococcus lactis of teh DNase(Streptodornase)encoding gene from Streptococcus equismilis H46A /
[ Wolinowska,R.;K.J.Ceglowskip;G.Venema ] / Gene   DOI   ScienceOn
6 Staphylococcal nuclease,chemical properties and catalysis /
[ Anfinsen,C.B.;P.Cuatrecasas;H,Taniuchi;P.Boyer(ed.); ] / The Enzymes   DOI
7 Classical mutagenisis techniques /
[ Lawrence,C.W. ] / Methods in Enzymol.
8 Expression and regulation of the streptokinase gene /
[ Malke,H.;K.Stenier;K.Gase;C.Frank ] / Methods   DOI   ScienceOn
9 Methylgreen.A DNA major groove binding drug /
[ Kim,S.K.;B.Norden ] / FEBS.   DOI   ScienceOn
10 Nucleotide sequence of the streptokinase gene from Streptococcus equisimilis H46A /
[ Malke,H.;B.Roe;J.J.Ferretti ] / Gene   DOI
11 Cloning of the hexA mismatch-repair gene of Streptococcus pneumoniae and identification of the product /
[ Martin,B.;H.Prats;J.P.Claverys ] / Gene   DOI
12 High-level expression and secretion of Streptokinase in Escherichia coli /
[ Ko,J.H.;D.D.Park;I.C.Kim;S.H.Lee;S.M.Byan ] / Biotechnol. Lett.   DOI   ScienceOn
13 The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli /
[ Ball,T.K.;P.N.Saurugger;M.J.Benedik ] / Gene   DOI
14 Streptococcal deoxyribonuclease: significance in lysis of purulent exudates and production by strains of hemolytic Streptococci /
[ Tillet,W.S.;S.Scherry;L.R.Christensen ] / Proc. Soc. Exp. Biol. Med.   DOI
15 A procedure for the isolation of deoxyribonucleic acid from microorganisms /
[ Marmur,J. ] / J. Mol. Biol.   DOI
16 Formation of competent Bacillus subtilis cells /
[ Sadaie,Y.;T.Kada ] / J. Bacteriol.
17 High level expression of Streptokinase in Escherichia coli /
[ Estrada,M.P.;L.Hernandez;A.Perez;Rodriguex;R.Serano;R.Rubiera;A.Pedraza;G.Padron;W.Antuch;J.de la Fuente;L.Herrera ] / Biotechnol.   DOI   ScienceOn
18 A genetic system for analysis of staphylococcal nuclase /
[ Sortle,D. ] / Gene   DOI