• Title/Summary/Keyword: Co-stimulation

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Antioxidant and Antiatopic Effects of Refined Chaff Liquid Smoke (정제 왕겨초액의 항산화 및 항아토피 활성)

  • Son, Hyeong-U;Heo, Jin-Chul;Kim, Suk-Kyung;Kwon, Seon-Young;Han, Gil-Hwan;Lee, Sang-Han
    • Food Science and Preservation
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    • v.18 no.2
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    • pp.266-270
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    • 2011
  • The refined chaff liquid smoke purified by the second or third filtering, supplemented with Artemisia extract or Perillae folium extract was investigated by measuring antioxidant and antiatopic activities. In DPPH and FRAP assays, three kinds of chaff liquid smokes had potential in antioxidant activity. Additionally, we assessed for antiatopic activity by using C57BLl6 mice with stimulation of dinitrofluorobenzene and observed that their ear swelling was alleviated by various fractions. Overall, all of the chaff liquid smoke reduced the response of swelling on ear epidermis. Especially, refined chaff liquid smoke supplemented with Perillae folium extract was most active than any other fractions.

Expression of Co-stimulatory Molecules and STAT/SOCS Signaling Factors in the Splenocytes of Mice Tolerized against Arthritis by Oral Administration of Type II Collagen (제2형 콜라겐으로 경구관용을 유도한 관절염 모델 마우스의 비장림프구내의 보조자극인자 및 STAT/SOCS 신호전달 인자의 발현 양상조사)

  • Lee, Kang-Eun;Hwang, Sue-Yun;Min, So-Youn;Kim, Ho-Youn
    • IMMUNE NETWORK
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    • v.3 no.3
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    • pp.248-254
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    • 2003
  • Oral administration of antigen has long been used in the induction of immune tolerance in various animal models of autoimmune diseases including rheumatoid arthritis (RA). Alleveation of arthritogenic symptoms has been reported from RA patients who received oral administration of type II collagen (CII) without side effects, however its rather inconsistent therapeutic efficacy and variation among patients calls for more detailed investigation on the mechanism of oral tolerance to be settled as regular treatment for RA. In an attempt to understand the immunogenic processes underpinning tolerance induction by orally administered CII, we analyzed changes in the expression of costimulatory molecules and STAT/SOCS signaling messengers in the mouse model of collagen induced arthritis (CIA). We found thatin the spleen of CIA mice, that has been undergone repeated oral feeding of CII prior to the induction of arthritis, showed increased promortion of CTLA4 expressing lymphocytes than in the spleen of PBS fed control. On the other hand, cells expressing CD28 or ICOS were decreased in the spleen of tolerized mice. Tolerance induction by oral CII administration also enhanced the expression of STAT6 in both RNA and protein level, while not affecting the expression of STAT3. The expression of SOCS3, which hasbeen known to transmit STAT-mediated signals from Th2 type cytokines, remained unchanged in the spleen of tolerized mice. Interestingly transcript of SOCS1, which has been associated with Th1 related pathways, was only visible in the spleen of tolerized but not of control mice, suggesting that as in the case of IL-6 signaling, it may exert a feed back inhibition toward the Th1 type stimulation.

Theoretical Bases and Technical Application of Breathing Therapy in Stress Management (스트레스 관리 시 호흡치료의 이론적 근거와 기법 적용)

  • 이평숙
    • Journal of Korean Academy of Nursing
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    • v.29 no.6
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    • pp.1304-1313
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    • 1999
  • Breathing is essential for life and at the same time takes a role as a antidote for stress. In the Orient, it was recognized early that respiration, mind, and body have a relation that is inseparable and therefore proper breathing is so important. However, since the mechanism of therapeutic effect by breathing have not been verified, the treatment has been continued till recent years. From that which originated in the Orient, several techniques in the west have been developed to regulate breathing, and have been applying to the clinical situation and to studies, however scientific studies are still lacking. Recently, relaxed breathing has been used as an efficient strategy for breathing therapy as it has an effect on reducing physiological tension and arousal, and, therefore can be used as a basic technique to control or manage stress. In this study, in order to provide basic information and guidelines for clinical application, which will aid in the application of the theoretical basics of breathing therapy and its technique, a review of the literative was conducted. The findings are as follows: 1. Since proper breathing not only has, physically, the important function in supplying oxygen to the body but also gives a good emotional, or pleasant state of mind, it is the first step in controlling physical and mental health. 2. The basic types of breathing can be classified into two types; ‘diaphragmatic breathing(relaxed breathing)’ and ‘chest breathing(stress breathing)’. In yoga type breathing, there are four kinds of breathing, ‘upper breathing’, ‘mid breathing’, ‘down breathing’, and ‘complete breathing’. 3. The theoretical explanation of the positive thera peutic effect of breathing therapy techniques exemplifies good brain function, sufficient air flow through the nasal passages, diaphragmatic movement, light vagal stimulation, CO2 changes and cognitive diversion but in most studies, the hypothesis of CO2 is supported. 4. The technique of breathing is designated with many names according to the muscles and techniques used for breathing, and for control of stress, diaphragmatic breathing(relaxed breathing) is explained as a basic technique best used to manage of stress. 5. The relaxed-breathing includes slow diaphragmatic breathing, breath meditation, nasal breathing, yogic abdominal breathing, Benson's relaxed response, and quiet response.

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A comprehensive review of the comparative studies of Korean(Panax ginseng) and American(Panax quinquefolium) Ginsengs on the Thermogenic Response (고려인삼(Panax ginseng)과 미국삼(Panax quinquefolium)의 승열작용에 대한 비교 고찰)

  • Nam, Ki Yeul;Yang, Byung Wook;Shin, Wang Soo;Park, Jong Dae
    • Korean Journal of Pharmacognosy
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    • v.49 no.3
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    • pp.203-218
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    • 2018
  • It has been extensively known in the high temperature regions such as southern China and south east Asia that Korean ginseng(Panax ginseng) intake raises body heat, while American ginseng(Panax quinquefolium) lowers it, based upon oriental medicine. This is likely due to the misunderstanding of the conception that Korean ginseng shows a warm property, resulting in enhanced blood circulation and stimulation of metabolism. It's further supported by the fact that current scientific studies have shown no notable side effects. Moreover, it has been demonstrated in animal experimental test that Korean ginseng shows a complementary effect in a high temperature environment, together with scientific evidence of a preventive effect of ginseng saponin against cold conditions, suggesting it to exhibit an adaptogenic effect normalizing the body under abnormal body conditions. However, there is a shortage of studies published concerning comparative clinical and animal studies of Korean and American ginsengs, and also, on natives of high temperature countries. Therefore, a further placebo-controlled clinical study has to be considered to elucidate scientifically clinical evaluation of Korean and American ginsengs on the body heat. In this article, we summarize the scientific publications reporting the clinical comparative study of Korean and American ginsengs on body heat response.

Study of Producing Natural Gas From Gas Hydrate With Industrial Flue Gas (산업용 배기가스를 이용한 가스 하이드레이트로부터의 천연가스 생산 연구)

  • Seo, Yu-Taek;Kang, Seong-Pil;Lee, Jae-Goo;Cha, Min-Jun;Lee, Huen
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.10a
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    • pp.188-191
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    • 2008
  • There have been many methods for producing natural gas from gas hydrate reservoirs in permafrost and sea floor sediments. It is well knownthat the depressurization should be a best option for Class 1 gas hydrate deposit, which is composed of tow layers: hydrate bearing layer and an underlying free gas. However many of gas hydrate reservoirs in sea floor sediments are classified as Class 2 that is composed of gas hydrate layer and mobile water, and Class 3 that is a single gas hydrate layer. The most appropriate production methods among the present methods such as thermal stimulation, inhibitor injection, and controlled oxidation are still under development with considering the gas hydrate reservoir characteristics. In East Sea of Korea, it is presumed that the thick fractured shale deposits could be Class 2 or 3, which is similar to the gas hydrate discovered offshore India. Therefore it is needed to evaluate the possible production methods for economic production of natural gas from gas hydrate reservoir. Here we would like to present the production of natural gas from gas hydrate deposit in East Sea with industrial flue gases from steel company, refineries, and other sources. The existing industrial complex in Gyeongbuk province is not far from gas hydrate reservoir of East Sea, thus the carbon dioxide in flue gas could be used to replace methane in gas hydrate. This approach is attractive due to the suggestion of natural gas productionby use of industrial flue gas, which contribute to the reduction of carbon dioxide emission in industrial complex. As a feasibility study, we did the NMR experiments to study the replacement reaction of carbon dioxide with methane in gas hydrate cages. The in-situ NMR measurement suggeststhat 42% of methane in hydrate cages have been replaced by carbon dioxide and nitrogen in preliminary test. Further studies are presented to evaluate the replacement ratio of methane hydrate at corresponding flue gas concentration.

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Studies on Cellulolytic Enzymes Produced by Aspergillus saitoi -II. Influence of Various Media Condition on Cellulase Production- (Aspergillus saitoi가 생산(生産)하는 섬유소(纖維素) 분해(分解) 효소(酵素)에 관(關)한 연구(硏究) -제 2 보(第 2 報) 각종(各種) 배지조건(培地條件)이 Cellulase 생성(生成)에 미치는 영향에 관하여-)

  • Lee, Soon-Ae;Oh, Suk-Hen;Youn, Jung-Eui
    • Korean Journal of Food Science and Technology
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    • v.3 no.3
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    • pp.185-188
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    • 1971
  • This experiment was conducted to study on the productive conditions of cellulase by Aspergillus saitoi in the shaking culture medium. The results were as follows: 1. The production of enzyme required higher concentration of corn steep liquor than that of dextrin. 2. The concentration of 1.0% $NH_4H_2SO_4$ produced the enzyme excellently than 3.0%. 3. The cellulase was produced very slowly by adding $(NH_4)_2SO_4$, but the final concentration of the enzyme was higher than control. The production was suppressed by addition of $CaCO_3$. 4. The addition of $1.0{\sim}2.0%$ substrate caused an increase or stimulation in cellulase production.

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Cooperative stimulation of cisplatin-mediated apoptosis by hepatitis B virus X Protein and hepatitis C virus core Protein (B형 간염 바이러스 X 단백질과 C형 간염 바이러스의 코어 단백질에 의한 cisplatin-매개성 세포 예정사의 협조적 촉진)

  • Kwun, Hyun-Jin;Jang, Kyung-Lib
    • Journal of Life Science
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    • v.17 no.6 s.86
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    • pp.766-771
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    • 2007
  • The co-infection with hepatitis B virus (HBV) and hepatitis C Virus (HCV) is associated with a more severe liver disease and increased frequency in the development of hepatocellular carcinoma com-pared to those with single infection. Here, we demonstrated that HBV X protein (HBx) and HCV Core cooperatively up-regulated the level of p53 in human hepatoma HepG2 cells. The elevated p53 subsequently stimulated the expression of proapoptotic Bax whereas it repressed the expression of antiapoptotic Bcl2. These effects, however, were not observed in p53-negative Hep3B cells. Consistently to their cooperative regulation of apoptotic effectors, HBx and HCV Core additively stimulated cisplatin-mediated apoptotic cell death of HepG2 but not of Hep3B cells. These results may help to explain the development of a more severe liver disease in patients co-infection with HBV and HCV as well as some contradictory results on the roles of HBx and Core in apoptosis.

Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis

  • Mendoza-Oliveros, Tahali;Arana-Argaez, Victor;Alvarez-Sanchez, Leidi C.;Lara-Riegos, Julio;Alvarez-Sanchez, Maria Elizbeth;Torres-Romero, Julio C.
    • Parasites, Hosts and Diseases
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    • v.57 no.1
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    • pp.33-38
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    • 2019
  • Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis, which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis, which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTRPV), as a promising immunogen in BALB/c mice. First, TvTRPV was cloned and expressed as a recombinant protein in Escherichia coli BL21 cells and purified by nickel affinity. Next, BALB/c mice were immunized and the antibody levels in mice serum and cytokines from the supernatant of macrophages and from co-culture systems were evaluated. Recombinant TvTRPV triggered high levels of specific total IgG in sera from the immunized mice. Also, a statistically significant increase of cytokines: $IL-1{\beta}$, IL-6, and $TNF-{\alpha}$ after stimulation with the corresponding antigens in vitro, was identified. Moreover, co-cultures using $CD4^+$ T cells from immunized mice were able to identify higher levels of IL-10 and $IFN-{\gamma}$. These results were useful to validate the immunogenicity of TvTRPV in BALB/c mice, where IL-10-$IFN-{\gamma}$-secreting cells could play a role in infection control, supporting the potential of TvTRPV as a promising target for vaccine against T. vaginalis.

Ethanol Extracts from the Roots of Reed Prevent Skin Hyperpigmentation, Wrinkle Formation and Dryness

  • Sung Hyeok Kim;Sohee Jang;Hyun Jung Koo;Seung Namkoong;Sungsil Hong;Mi-Ja Kim;Chang Woo Ha;Hyosun Lim;Youn Kyu Kim;Eun-Hwa Sohn
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.49-49
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    • 2021
  • The roots of reed (Phragmites australis) were used in traditional medicine to treat respiratory problems, including symptoms such as high fever and cough. In this study, we identified the active ingredient from 70% EtOH reed root extract, and evaluated the whitening, wrinkle improvement and moisturizing effects. The content of p-coumaric acid, the active ingredient of the roots of P. australis, was slightly lower in 70% EtOH extract than in 100% EtOH extract. However, 70% EtOH reed root extract showed similar or higher effect in reducing power, DPPH, hydrogen peroxide scavenging, and nitric oxide scavenging activity compared to 100% EtOH extract. Moreover, 70% EtOH reed root extract markedly inhibited melanogenesis in B16F10 cells treated with α-melanocyte-stimulating hormone. 70% EtOH reed root extract significantly inhibited the mRNA expression of matrix metalloproteinase-1 (MMP-1) and reduced elastase activity in HDF human dermal fibroblasts. In addition, 70% EtOH reed root extract ameliorated hyaluronic acid synthase-2 (HAS-2) expression induced by ultraviolet B (UVB) stimulation in HaCaT keratinocytes. The results of this study suggest that 70% EtOH reed root extract has potential as a functional cosmetic material related to whitening, wrinkle improvement, and moisturizing.

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Anti-lipogenic Effects of Tannic Acid in 3T3-L1 Adipocytes and in High Fat Diet-fed Rats

  • Kim, Hyun-Ju;Yun, Ye-Rang;Song, Yeong-Bok;Song, Yeong-Ok
    • Food Science and Biotechnology
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    • v.17 no.2
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    • pp.362-366
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    • 2008
  • Anti-lipogenic effects of tannic acid on 3T3-L1 adipocytes as well as on rats fed high fat diet (HFD) were investigated. Tannic acid stimulated lipolysis through suppression of the leptin secretion and an increase of glycerol release in a dose dependent manner in 3T3-L1 adipocytes. For animal study, the rats were fed either HFD or HFD supplemented with 1%(w/w) tannic acid (HFDT) for 12 weeks, respectively. Body weight gain, liver weight, and visceral fat mass in rats fed HFDT were significantly decreased compared to those of rats fed HFD. The lipid profiles of HFDT group were significantly decreased compared with HFD group in the serum and liver, whereas fecal total cholesterol excretion was increased in HFDT group. These results suggest that anti-lipogenic effect of tannic acid in 3T3-L1 adipocytes and in rats fed HFD may be due to the stimulation of lipolysis and the reduction of lipid levels.