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http://dx.doi.org/10.5352/JLS.2007.17.6.766

Cooperative stimulation of cisplatin-mediated apoptosis by hepatitis B virus X Protein and hepatitis C virus core Protein  

Kwun, Hyun-Jin (Division of Biological Sciences, College of Natural Sciences, Pusan National University)
Jang, Kyung-Lib (Division of Biological Sciences, College of Natural Sciences, Pusan National University)
Publication Information
Journal of Life Science / v.17, no.6, 2007 , pp. 766-771 More about this Journal
Abstract
The co-infection with hepatitis B virus (HBV) and hepatitis C Virus (HCV) is associated with a more severe liver disease and increased frequency in the development of hepatocellular carcinoma com-pared to those with single infection. Here, we demonstrated that HBV X protein (HBx) and HCV Core cooperatively up-regulated the level of p53 in human hepatoma HepG2 cells. The elevated p53 subsequently stimulated the expression of proapoptotic Bax whereas it repressed the expression of antiapoptotic Bcl2. These effects, however, were not observed in p53-negative Hep3B cells. Consistently to their cooperative regulation of apoptotic effectors, HBx and HCV Core additively stimulated cisplatin-mediated apoptotic cell death of HepG2 but not of Hep3B cells. These results may help to explain the development of a more severe liver disease in patients co-infection with HBV and HCV as well as some contradictory results on the roles of HBx and Core in apoptosis.
Keywords
Apoptosis; Cisplatin; Core; HBV; HBx; HCV;
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